EN1 ORF clone pFN21AB9715

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9715

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Clone info.

Species

human

Clone Name

pFN21AB9715 Sequence (with vector) Restriction Map

Product ID

FHC04893

ORF Sequence

DNA sequence (1176 bp)
Protein sequence (392 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB9715

Symbol

EN1

Alias : ENDOVESLB

Description

engrailed homeobox 1

Original Clone ID

cp00298

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1176 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 392 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2123.1 7.9e-59 100.0 EN1 CCDS5940.1 1.4e-13 47.1 EN2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000747 302 319 PR00026 Homeobox engrailed IPR020479 325 336 PR00024 Homeobox IPR020479 340 350 PR00024 Homeobox IPR020479 350 359 PR00024 Homeobox IPR000747 365 382 PR00026 Homeobox engrailed HMMPfam IPR001356 304 360 PF00046 Homeobox IPR019549 361 392 PF10525 Homeobox engrailed HMMSmart IPR001356 303 365 SM00389 Homeobox ProfileScan IPR001356 301 361 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001417 392 131290 100.0 100.0 NP_001418 333 131310 47.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9715 Download

>KIBB9715  1176 bp
ATGGAAGAACAGCAGCCGGAACCTAAAAGTCAGCGCGACTCGGCCCTCGGCGCGGCGGCG
GCGGCGACTCCGGGCGGCCTCAGCCTGAGCCTCAGTCCGGGCGCCAGCGGCAGCAGCGGC
AGCGGCAGCGATGGAGACAGCGTGCCGGTGTCCCCGCAGCCTGCGCCCCCCTCGCCGCCC
GCGGCGCCTTGCCTGCCGCCCCTGGCCCACCACCCGCACCTCCCCCCACACCCCCCGCCC
CCGCCGCCTCAGCATCTCGCGGCGCCTGCTCACCAGCCGCAGCCAGCGGCCCAGCTGCAC
CGCACCACCAACTTTTTCATCGACAACATCCTGAGGCCGGACTTCGGCTGCAAAAAGGAG
CAGCCGCCACCGCAGCTTCTGGTGGCTGCGGCGGCCAGAGGAGGCGCAGGAGGAGGAGGC
CGGGTCGAGCGTGACAGAGGCCAGACTGCCGCAGGTAGAGACCCTGTCCACCCGTTGGGC
ACCCGGGCGCCAGGCGCTGCCTCGCTCCTGTGCGCCCCGGACGCGAACTGTGGCCCACCC
GACGGCTCCCAGCCAGCCGCCGCCGGCGCGGGCGCGTCTAAAGCTGGGAACCCGGCTGCG
GCGGCGGCGGCGGCCGCGGCGGCAGTGGCGGCGGCGGCGGCGGCCGCAGCAGCCAAGCCC
TCGGACACCGGTGGCGGCGGCAGTGGAGGCGGCGCGGGGAGCCCCGGAGCGCAGGGCACC
AAATACCCGGAGCACGGCAACCCGGCTATCCTACTTATGGGCTCAGCCAACGGCGGGCCC
GTGGTCAAAACTGACTCGCAGCAGCCTCTCGTATGGCCCGCCTGGGTGTACTGCACACGT
TATTCGGATCGTCCATCCTCCGGTCCGCGCACCAGGAAGCTGAAGAAGAAGAAGAACGAG
AAGGAGGACAAGCGGCCGCGGACCGCGTTCACGGCCGAGCAGCTGCAGAGACTCAAGGCG
GAGTTCCAGGCAAACCGCTACATCACGGAGCAGCGGCGGCAGACCCTGGCCCAGGAACTC
AGCCTCAACGAGTCCCAGATCAAGATCTGGTTCCAGAACAAGCGCGCCAAGATCAAGAAA
GCCACAGGCATCAAGAACGGCCTGGCGCTGCACCTCATGGCCCAGGGACTGTACAACCAC
TCCACCACCACGGTCCAGGACAAAGACGAGAGCGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB9715 Download

>KIBB9715  392 aa
MEEQQPEPKSQRDSALGAAAAATPGGLSLSLSPGASGSSGSGSDGDSVPVSPQPAPPSPP
AAPCLPPLAHHPHLPPHPPPPPPQHLAAPAHQPQPAAQLHRTTNFFIDNILRPDFGCKKE
QPPPQLLVAAAARGGAGGGGRVERDRGQTAAGRDPVHPLGTRAPGAASLLCAPDANCGPP
DGSQPAAAGAGASKAGNPAAAAAAAAAAVAAAAAAAAAKPSDTGGGGSGGGAGSPGAQGT
KYPEHGNPAILLMGSANGGPVVKTDSQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNE
KEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKK
ATGIKNGLALHLMAQGLYNHSTTTVQDKDESE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9715 Download

>pFN21AB9715  5881 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAGAACAGCAGCCGGAACCTAAAAGTCAGCG
CGACTCGGCCCTCGGCGCGGCGGCGGCGGCGACTCCGGGCGGCCTCAGCCTGAGCCTCAG
TCCGGGCGCCAGCGGCAGCAGCGGCAGCGGCAGCGATGGAGACAGCGTGCCGGTGTCCCC
GCAGCCTGCGCCCCCCTCGCCGCCCGCGGCGCCTTGCCTGCCGCCCCTGGCCCACCACCC
GCACCTCCCCCCACACCCCCCGCCCCCGCCGCCTCAGCATCTCGCGGCGCCTGCTCACCA
GCCGCAGCCAGCGGCCCAGCTGCACCGCACCACCAACTTTTTCATCGACAACATCCTGAG
GCCGGACTTCGGCTGCAAAAAGGAGCAGCCGCCACCGCAGCTTCTGGTGGCTGCGGCGGC
CAGAGGAGGCGCAGGAGGAGGAGGCCGGGTCGAGCGTGACAGAGGCCAGACTGCCGCAGG
TAGAGACCCTGTCCACCCGTTGGGCACCCGGGCGCCAGGCGCTGCCTCGCTCCTGTGCGC
CCCGGACGCGAACTGTGGCCCACCCGACGGCTCCCAGCCAGCCGCCGCCGGCGCGGGCGC
GTCTAAAGCTGGGAACCCGGCTGCGGCGGCGGCGGCGGCCGCGGCGGCAGTGGCGGCGGC
GGCGGCGGCCGCAGCAGCCAAGCCCTCGGACACCGGTGGCGGCGGCAGTGGAGGCGGCGC
GGGGAGCCCCGGAGCGCAGGGCACCAAATACCCGGAGCACGGCAACCCGGCTATCCTACT
TATGGGCTCAGCCAACGGCGGGCCCGTGGTCAAAACTGACTCGCAGCAGCCTCTCGTATG
GCCCGCCTGGGTGTACTGCACACGTTATTCGGATCGTCCATCCTCCGGTCCGCGCACCAG
GAAGCTGAAGAAGAAGAAGAACGAGAAGGAGGACAAGCGGCCGCGGACCGCGTTCACGGC
CGAGCAGCTGCAGAGACTCAAGGCGGAGTTCCAGGCAAACCGCTACATCACGGAGCAGCG
GCGGCAGACCCTGGCCCAGGAACTCAGCCTCAACGAGTCCCAGATCAAGATCTGGTTCCA
GAACAAGCGCGCCAAGATCAAGAAAGCCACAGGCATCAAGAACGGCCTGGCGCTGCACCT
CATGGCCCAGGGACTGTACAACCACTCCACCACCACGGTCCAGGACAAAGACGAGAGCGA
GGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
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