################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BpmI BpuAI BsaAI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI DraI DraII DraIII DrdI Eam1105I EarI Eco47III EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp509I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 835 ACACGT A^C[GA][CT]GT AgeI 667 ACCGGT A^CCGGT AlwI 846 GGATC GGATC(4/5) AocI 330 CCTGAGG CC^T[ACGT]AGG ApaI 775 GGGCCC GGGCC^C AspHI 730 GAGCAC G[AT]GC[AT]^C AspI 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 482 CCCGGG C^[CT]CG[GA]G BglI 613 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1043 AGATCT A^GATCT BlpI 761 GCTCAGC GC^T[ACGT]AGC Bpu1102I 761 GCTCAGC GC^T[ACGT]AGC BsaBI 1038 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 458 GAGACC GGTCTC(1/5) BsiHKAI 730 GAGCAC G[AT]GC[AT]^C BsoBI 482 CCCGGG C^[CT]CG[GA]G Bsp1407I 1130 TGTACA T^GTACA BspMI 451 GCAGGT ACCTGC(4/8) BsrGI 1130 TGTACA T^GTACA BssHII 1062 GCGCGC G^CGCGC BstYI 1043 AGATCT [GA]^GATC[CT] Bsu36I 330 CCTGAGG CC^T[ACGT]AGG CelII 761 GCTCAGC GC^T[ACGT]AGC Eco81I 330 CCTGAGG CC^T[ACGT]AGG EcoNI 1005 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HphI 270 TCACC GGTGA(8/7) MaeII 837 ACGT A^CGT MaeIII 431 GTGAC ^GT[ACGT]AC MamI 1038 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1043 AGATCT [GA]^GATC[CT] NaeI 562 GCCGGC GCC^GGC NgoMIV 562 GCCGGC G^CCGGC NlaIII 1116 CATG CATG^ PflFI 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 468 CCACCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 667 ACCGGT A^CCGGT PstI 943 CTGCAG CTGCA^G SfcI 943 CTGCAG C^T[GA][CT]AG SmaI 482 CCCGGG CCC^GGG SmlI 952 CTCAAG C^T[CT][GA]AG SspBI 1130 TGTACA T^GTACA Tsp45I 431 GTGAC ^GT[GC]AC Tth111I 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 468 CCACCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1043 AGATCT [GA]^GATC[CT] XmaCI 482 CCCGGG C^CCGGG XmaI 482 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 828 GTAC GT^AC 1131 GTAC BbvCI 78 CCTCAGC CCTCAGC(-5/-2) 247 CCTCAGC BsaWI 667 ACCGGT [AT]^CCGG[AT] 859 TCCGGT BstF5I 327 CATCC GGATG(2/0) 854 CATCC CpoI 861 CGGTCCG CG^G[AT]CCG 919 CGGACCG FokI 327 CATCC GGATG(9/13) 854 CATCC HgaI 520 GACGC GACGC(5/10) 574 GCGTC PvuII 292 CAGCTG CAG^CTG 940 CAGCTG RsaI 828 GTAC GT^AC 1131 GTAC RsrII 861 CGGTCCG CG^G[AT]CCG 919 CGGACCG SfaNI 252 GCATC GCATC(5/9) 1088 GCATC SgrAI 561 CGCCGGCG C[GA]^CCGG[CT]G 666 CACCGGTG TaqI 320 TCGA T^CGA 425 TCGA TspRI 623 CAGTG CA[GC]TG[ACGT][ACGT]^ 681 CAGTG TthHB8I 320 TCGA T^CGA 425 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV NotI PleI SacII Sau3AI Sau96I ScrFI SfoI TseI