(Circular) (Six-base) MAPSORT of: pFN21AB9715.seq Check: 6278 from: 1 to: 5881 pFN21AB9715 5881 bp With 182 enzymes: SgfI * March 28, 2012 12:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 3944 5328 5596 1632 Size: 1274 1038 1384 268 1917 Fragments arranged by size: 1917 1384 1274 1038 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 2840 4780 5757 1203 Size: 1637 1940 977 1327 Fragments arranged by size: 1940 1637 1327 977 AgeI A'CCGG_T Cuts at: 2672 4712 2672 Size: 2040 3841 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2383 2501 3010 3133 3624 5196 2383 Size: 118 509 123 491 1572 3068 Fragments arranged by size: 3068 1572 509 491 123 118 ApaI G_GGCC'C Cuts at: 2784 2784 Size: 5881 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 3190 3416 3568 5677 1493 Size: 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2487 3206 1870 Size: 73 544 719 4545 Fragments arranged by size: 4545 719 544 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2188 2266 2483 2491 Size: 325 217 627 318 83 78 217 8 Cuts at: 2491 2720 3202 4657 618 Size: 229 482 1455 1842 Fragments arranged by size: 1842 1455 627 482 325 318 229 217 217 83 78 8 BanII G_rGCy'C Cuts at: 1391 2708 2768 2784 3200 1391 Size: 1317 60 16 416 4072 Fragments arranged by size: 4072 1317 416 60 16 BbeI G_GCGC'C Cuts at: 2109 2192 2270 2495 2109 Size: 83 78 225 5495 Fragments arranged by size: 5495 225 83 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BbvCI CC'TCA_GC Cuts at: 2084 2253 2084 Size: 169 5712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2624 4492 136 Size: 107 122 71 631 605 952 1868 1525 Fragments arranged by size: 1868 1525 952 631 605 122 107 71 BglII A'GATC_T Cuts at: 3048 5876 3048 Size: 2828 3053 BlpI GC'TnA_GC Cuts at: 2767 3298 2767 Size: 531 5350 Bme1580I G_kGCm'C Cuts at: 1790 2486 2723 2784 3942 5098 1790 Size: 696 237 61 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 696 237 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4541 1239 Size: 3302 2579 Bpu10I CC'TnA_GC Cuts at: 2084 2090 2253 2084 Size: 6 163 5712 Fragments arranged by size: 5712 163 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2942 4006 4871 5169 2942 Size: 1064 865 298 3654 Fragments arranged by size: 3654 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 4544 882 Size: 617 958 2087 2219 Fragments arranged by size: 2219 2087 958 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 3047 5875 3047 Size: 2828 3053 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2189 2267 2492 Size: 53 83 186 936 573 83 78 225 Cuts at: 2492 3688 4070 4705 275 Size: 1196 382 635 1451 Fragments arranged by size: 1451 1196 936 635 573 382 225 186 83 83 78 53 BsaWI w'CCGG_w Cuts at: 2672 2864 4314 4712 4986 5133 5544 2672 Size: 192 1450 398 274 147 411 3009 Fragments arranged by size: 3009 1450 411 398 274 192 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2130 2160 2412 2442 3128 Size: 30 165 30 727 30 252 30 686 Cuts at: 3128 3158 1178 Size: 30 3901 Fragments arranged by size: 3901 727 686 252 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2418 2430 2433 1186 Size: 1232 12 3 4634 Fragments arranged by size: 4634 1232 12 3 BseYI C'CCAG_C Cuts at: 2295 2553 2588 5084 5580 2295 Size: 258 35 2496 496 2596 Fragments arranged by size: 2596 2496 496 258 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2821 3098 1163 Size: 573 549 536 277 3946 Fragments arranged by size: 3946 573 549 536 277 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2619 2649 2921 2940 3331 4092 Size: 275 186 618 30 272 19 391 761 Cuts at: 4092 5120 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 618 391 275 272 186 30 19 BsiHKAI G_wGCw'C Cuts at: 2739 3942 4027 5098 2739 Size: 1203 85 1071 3522 Fragments arranged by size: 3522 1203 1071 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2486 2708 2723 2739 2768 2784 3200 Size: 399 696 222 15 16 29 16 416 Cuts at: 3200 3942 4027 5098 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 696 416 399 222 85 29 16 16 15 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2447 3236 844 Size: 1603 789 3489 Fragments arranged by size: 3489 1603 789 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 2567 2672 4525 4712 1700 Size: 110 339 418 105 1853 187 2869 Fragments arranged by size: 2869 1853 418 339 187 110 105 BsrGI T'GTAC_A Cuts at: 96 3135 96 Size: 3039 2842 BssHII G'CGCG_C Cuts at: 1925 3067 1925 Size: 1142 4739 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstYI r'GATC_y Cuts at: 1972 3048 3211 3578 3963 3980 5432 5518 5530 Size: 1076 163 367 385 17 1452 86 12 Cuts at: 5530 5876 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 385 367 346 163 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2336 Size: 5881 BtgI C'CryG_G Cuts at: 513 1065 2004 2184 2619 2784 2921 3153 513 Size: 552 939 180 435 165 137 232 3241 Fragments arranged by size: 3241 939 552 435 232 180 165 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2148 487 Size: 683 56 922 4220 Fragments arranged by size: 4220 922 683 56 BtsI GCAGTG_nn' Cuts at: 2634 2692 3397 4191 4211 2634 Size: 58 705 794 20 4304 Fragments arranged by size: 4304 794 705 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2616 2646 Size: 54 1425 50 275 18 567 219 30 Cuts at: 2646 2918 2937 3328 4217 5846 8 Size: 272 19 391 889 1629 43 Fragments arranged by size: 1629 1425 889 567 391 275 272 219 54 50 43 30 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2616 2646 2918 2937 3328 1537 Size: 275 804 30 272 19 391 4090 Fragments arranged by size: 4090 804 391 275 272 30 19 EarI CTCTTCn'nnn_ Cuts at: 1353 3811 1353 Size: 2458 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2978 4468 4842 4988 387 Size: 1064 138 1389 1490 374 146 1280 Fragments arranged by size: 1490 1389 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2906 3944 4541 5328 5596 1239 Size: 393 1274 1038 597 787 268 1524 Fragments arranged by size: 1524 1274 1038 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3014 3014 Size: 5881 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2109 2192 2270 2495 2502 3113 5028 1678 Size: 431 83 78 225 7 611 1915 2531 Fragments arranged by size: 2531 1915 611 431 225 83 78 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 2412 2444 2454 Size: 32 135 32 511 32 297 32 10 Cuts at: 2454 2486 2843 2875 4522 4554 4596 4628 1373 Size: 32 357 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 511 357 297 135 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KasI G'GCGC_C Cuts at: 2105 2188 2266 2491 2105 Size: 83 78 225 5495 Fragments arranged by size: 5495 225 83 78 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 4198 4357 518 Size: 648 2673 359 159 2042 Fragments arranged by size: 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2121 2127 2186 2291 2299 2621 2923 Size: 506 9 6 59 105 8 322 302 Cuts at: 2923 2947 2998 3297 3974 5122 5367 1606 Size: 24 51 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 506 322 302 299 245 105 59 51 24 9 8 6 NaeI GCC'GGC Cuts at: 2569 2569 Size: 5881 NarI GG'CG_CC Cuts at: 2106 2189 2267 2492 2106 Size: 83 78 225 5495 Fragments arranged by size: 5495 225 83 78 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4390 Fragments arranged by size: 4390 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2567 2567 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 2616 2646 2918 3328 2616 Size: 30 272 410 5169 Fragments arranged by size: 5169 410 272 30 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 3127 1348 Size: 1779 4102 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4510 PfoI T'CCnGG_A Cuts at: 3159 3159 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3012 3044 3950 3982 5463 5495 3012 Size: 32 906 32 1481 32 3398 Fragments arranged by size: 3398 1481 906 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PspOMI G'GGCC_C Cuts at: 2780 2780 Size: 5881 PstI C_TGCA'G Cuts at: 830 2952 3233 830 Size: 2122 281 3478 Fragments arranged by size: 3478 2122 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2299 2947 2299 Size: 648 5233 RsrII CG'GwC_CG Cuts at: 2867 2925 2867 Size: 58 5823 SacII CC_GC'GG Cuts at: 2187 2622 2924 2187 Size: 435 302 5144 Fragments arranged by size: 5144 435 302 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2948 3229 4364 5045 5236 826 Size: 219 1903 281 1135 681 191 1471 Fragments arranged by size: 1903 1471 1135 681 281 219 191 SfoI GGC'GCC Cuts at: 2107 2190 2268 2493 2107 Size: 83 78 225 5495 Fragments arranged by size: 5495 225 83 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 2567 2672 1700 Size: 867 105 4909 Fragments arranged by size: 4909 867 105 SmaI CCC'GGG Cuts at: 1872 2489 3208 1872 Size: 617 719 4545 Fragments arranged by size: 4545 719 617 SmlI C'TyrA_G Cuts at: 820 1017 1943 2957 3985 4886 5148 820 Size: 197 926 1014 1028 901 262 1553 Fragments arranged by size: 1553 1028 1014 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 3320 513 Size: 552 939 1316 3074 Fragments arranged by size: 3074 1316 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 3908 3925 4078 4263 4708 325 Size: 1255 1238 1090 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1238 1090 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 3135 4127 Size: 274 80 33 51 1443 855 303 992 Cuts at: 4127 5839 96 Size: 1712 138 Fragments arranged by size: 1712 1443 992 855 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 5841 5873 2130 Size: 32 3679 32 2138 Fragments arranged by size: 3679 2138 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2468 1316 Size: 1152 4729 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 2487 3206 1870 Size: 617 719 4545 Fragments arranged by size: 4545 719 617 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI