(Linear) (Six-base) MAPSORT of: KIBB9715.seq Check: 1454 from: 1 to: 1176 KIBB9715.orf With 182 enzymes: SgfI * March 22, 2012 15:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1176 Size: 901 275 AflIII A'CryG_T Cuts at: 0 835 1176 Size: 835 341 AgeI A'CCGG_T Cuts at: 0 667 1176 Size: 667 509 AlwNI CAG_nnn'CTG Cuts at: 0 378 496 1005 1128 1176 Size: 378 118 509 123 48 Fragments arranged by size: 509 378 123 118 48 ApaI G_GGCC'C Cuts at: 0 779 1176 Size: 779 397 AvaI C'yCGr_G Cuts at: 0 482 1176 Size: 482 694 BanI G'GyrC_C Cuts at: 0 100 183 261 478 486 715 1176 Size: 100 83 78 217 8 229 461 Fragments arranged by size: 461 229 217 100 83 78 8 BanII G_rGCy'C Cuts at: 0 703 763 779 1176 Size: 703 60 16 397 Fragments arranged by size: 703 397 60 16 BbeI G_GCGC'C Cuts at: 0 104 187 265 490 1176 Size: 104 83 78 225 686 Fragments arranged by size: 686 225 104 83 78 BbvCI CC'TCA_GC Cuts at: 0 79 248 1176 Size: 79 169 928 Fragments arranged by size: 928 169 79 BglI GCCn_nnn'nGGC Cuts at: 0 619 1176 Size: 619 557 BglII A'GATC_T Cuts at: 0 1043 1176 Size: 1043 133 BlpI GC'TnA_GC Cuts at: 0 762 1176 Size: 762 414 Bme1580I G_kGCm'C Cuts at: 0 481 718 779 1176 Size: 481 237 61 397 Fragments arranged by size: 481 397 237 61 Bpu10I CC'TnA_GC Cuts at: 0 79 85 248 1176 Size: 79 6 163 928 Fragments arranged by size: 928 163 79 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 937 1176 Size: 937 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 452 1176 Size: 452 724 BsaBI GATnn'nnATC Cuts at: 0 1042 1176 Size: 1042 134 BsaHI Gr'CG_yC Cuts at: 0 101 184 262 487 1176 Size: 101 83 78 225 689 Fragments arranged by size: 689 225 101 83 78 BsaWI w'CCGG_w Cuts at: 0 667 859 1176 Size: 667 192 317 Fragments arranged by size: 667 317 192 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 125 155 407 437 1123 1153 1176 Size: 125 30 252 30 686 30 23 Fragments arranged by size: 686 252 125 30 30 30 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 413 425 428 1176 Size: 413 12 3 748 Fragments arranged by size: 748 413 12 3 BseYI C'CCAG_C Cuts at: 0 290 548 583 1176 Size: 290 258 35 593 Fragments arranged by size: 593 290 258 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 816 1093 1176 Size: 280 536 277 83 Fragments arranged by size: 536 280 277 83 BsiEI CG_ry'CG Cuts at: 0 614 644 916 935 1176 Size: 614 30 272 19 241 Fragments arranged by size: 614 272 241 30 19 BsiHKAI G_wGCw'C Cuts at: 0 734 1176 Size: 734 442 Bsp1286I G_dGCh'C Cuts at: 0 481 703 718 734 763 779 1176 Size: 481 222 15 16 29 16 397 Fragments arranged by size: 481 397 222 29 16 16 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 442 1176 Size: 442 734 BsrFI r'CCGG_y Cuts at: 0 144 562 667 1176 Size: 144 418 105 509 Fragments arranged by size: 509 418 144 105 BsrGI T'GTAC_A Cuts at: 0 1130 1176 Size: 1130 46 BssHII G'CGCG_C Cuts at: 0 1062 1176 Size: 1062 114 BstYI r'GATC_y Cuts at: 0 1043 1176 Size: 1043 133 Bsu36I CC'TnA_GG Cuts at: 0 331 1176 Size: 331 845 BtgI C'CryG_G Cuts at: 0 179 614 779 916 1148 1176 Size: 179 435 165 137 232 28 Fragments arranged by size: 435 232 179 165 137 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 143 1176 Size: 143 1033 BtsI GCAGTG_nn' Cuts at: 0 629 687 1176 Size: 629 58 489 Fragments arranged by size: 629 489 58 EaeI y'GGCC_r Cuts at: 0 392 611 641 913 932 1176 Size: 392 219 30 272 19 244 Fragments arranged by size: 392 272 244 219 30 19 EagI C'GGCC_G Cuts at: 0 611 641 913 932 1176 Size: 611 30 272 19 244 Fragments arranged by size: 611 272 244 30 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 973 1176 Size: 973 203 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 1176 Size: 901 275 EcoNI CCTnn'n_nnAGG Cuts at: 0 1009 1176 Size: 1009 167 HaeII r_GCGC'y Cuts at: 0 104 187 265 490 497 1108 1176 Size: 104 83 78 225 7 611 68 Fragments arranged by size: 611 225 104 83 78 68 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 78 110 407 439 449 481 838 870 Size: 78 32 297 32 10 32 357 32 Cuts at: 870 1176 Size: 306 Fragments arranged by size: 357 306 297 78 32 32 32 32 10 KasI G'GCGC_C Cuts at: 0 100 183 261 486 1176 Size: 100 83 78 225 690 Fragments arranged by size: 690 225 100 83 78 MspA1I CmG'CkG Cuts at: 0 107 116 122 181 286 294 616 918 Size: 107 9 6 59 105 8 322 302 Cuts at: 918 942 993 1176 Size: 24 51 183 Fragments arranged by size: 322 302 183 107 105 59 51 24 9 8 6 NaeI GCC'GGC Cuts at: 0 564 1176 Size: 564 612 NarI GG'CG_CC Cuts at: 0 101 184 262 487 1176 Size: 101 83 78 225 689 Fragments arranged by size: 689 225 101 83 78 NgoMIV G'CCGG_C Cuts at: 0 562 1176 Size: 562 614 NotI GC'GGCC_GC Cuts at: 0 611 641 913 1176 Size: 611 30 272 263 Fragments arranged by size: 611 272 263 30 PasI CC'CwG_GG Cuts at: 0 1122 1176 Size: 1122 54 PflMI CCAn_nnn'nTGG Cuts at: 0 474 1176 Size: 474 702 PfoI T'CCnGG_A Cuts at: 0 1154 1176 Size: 1154 22 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1007 1039 1176 Size: 1007 32 137 Fragments arranged by size: 1007 137 32 PspOMI G'GGCC_C Cuts at: 0 775 1176 Size: 775 401 PstI C_TGCA'G Cuts at: 0 947 1176 Size: 947 229 PvuII CAG'CTG Cuts at: 0 294 942 1176 Size: 294 648 234 Fragments arranged by size: 648 294 234 RsrII CG'GwC_CG Cuts at: 0 862 920 1176 Size: 862 58 256 Fragments arranged by size: 862 256 58 SacII CC_GC'GG Cuts at: 0 182 617 919 1176 Size: 182 435 302 257 Fragments arranged by size: 435 302 257 182 SfcI C'TryA_G Cuts at: 0 943 1176 Size: 943 233 SfoI GGC'GCC Cuts at: 0 102 185 263 488 1176 Size: 102 83 78 225 688 Fragments arranged by size: 688 225 102 83 78 SgrAI Cr'CCGG_yG Cuts at: 0 562 667 1176 Size: 562 105 509 Fragments arranged by size: 562 509 105 SmaI CCC'GGG Cuts at: 0 484 1176 Size: 484 692 SmlI C'TyrA_G Cuts at: 0 952 1176 Size: 952 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 813 1176 Size: 813 363 TatI w'GTAC_w Cuts at: 0 827 1130 1176 Size: 827 303 46 Fragments arranged by size: 827 303 46 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 125 157 1176 Size: 125 32 1019 Fragments arranged by size: 1019 125 32 Tth111I GACn'n_nGTC Cuts at: 0 463 1176 Size: 463 713 XmaI C'CCGG_G Cuts at: 0 482 1176 Size: 482 694 Enzymes that do cut: AcuI AflIII AgeI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV NotI PasI PflMI PfoI PpiI PspOMI PstI PvuII RsrII SacII SfcI SfoI SgrAI SmaI SmlI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BpmI BsaAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI XbaI XcmI XhoI XmnI ZraI