WIPI2 ORF clone pFN21AB8657

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB8657

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Clone info.

Species

human

Clone Name

pFN21AB8657 Sequence (with vector) Restriction Map

Product ID

FHC02912

Flexi Clone PID

FXC02912

ORF Sequence

DNA sequence (1362 bp)
Protein sequence (454 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463348]

Gene

KIBB8657

Symbol

WIPI2

Alias : ATG18B, Atg21, CGI-50, IDDSSA, WIPI-2

Description

WD repeat domain, phosphoinositide interacting 2, transcript variant 1

Original Clone ID

oc00320

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1362 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 454 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5339.1 4.7e-191 100.0 WIPI2 CCDS34593.1 9.6e-173 96.0 WIPI2 CCDS47531.1 7.9e-170 97.6 WIPI2 CCDS47532.1 1.6e-151 93.6 WIPI2 CCDS47533.1 6.5e-140 97.1 WIPI2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 199 226 PF00400 WD40 repeat HMMSmart IPR001680 191 231 SM00320 WD40 repeat IPR001680 234 276 SM00320 WD40 repeat IPR001680 316 358 SM00320 WD40 repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056425 454 609225 100.0 100.0 NP_057087 436 609225 96.0 100.0 NP_001028690 443 609225 97.6 100.0 NP_001028691 425 609225 93.6 100.0 NP_001028692 384 609225 97.1 84.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8657 Download

>KIBB8657  1362 bp
ATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGCCGGCCAGCTGCTCTTCGCCAACTTC
AACCAGGACAACACAGAAGTGAAAGGGGCATCAAGAGCAGCTGGTCTTGGCCGTCGCGCT
GTTGTCTGGTCCCTAGCTGTTGGTAGTAAGTCCGGTTATAAATTTTTCTCCCTTTCTTCT
GTGGATAAGCTGGAACAGATCTATGAATGCACCGATACGGAAGATGTGTGCATTGTAGAG
AGATTGTTCTCCAGCAGCCTAGTGGCCATCGTGAGCCTTAAAGCACCAAGGAAGCTAAAG
GTTTGCCACTTTAAGAAGGGAACTGAGATCTGCAACTACAGCTACTCCAACACGATTCTG
GCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATGCCTGGAGGAGTCCCTGTACATCCAC
AACATTCGGGACATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCTGCAGGC
CTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACC
ATCGGAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCGGCT
CACGACAGTCCTTTAGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCT
TCGGAGAAGGGGACCGTGATTAGGGTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAG
TTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAGCATGGACGGC
ATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTTCAAACTCGAGACTGTG
AAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTGCTCATG
GCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTC
GCCACGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATTCAG
AAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGAC
CCCCAGGAGGGCGGCGAGTGTGCCCTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAA
ACGACCAATGAGATCTTGGACTCTGCCTCTCACGACTGCCCCTTAGTCACTCAGACATAC
GGCGCAGCTGCAGGAAAAGGTACTTACGTGCCTTCATCCCCAACGAGACTTGCCTACACA
GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTG
GATGAGGACAGCGAGCACCCGCCCATGATTCTTCGGACTGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB8657 Download

>KIBB8657  454 aa
MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSS
VDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTIL
AVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSAT
IGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFE
FRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKVLM
ASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLD
PQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYT
DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8657 Download

>pFN21AB8657  6067 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACCTGGCGAGCCAGAGCGGGGAGGCCGGCGC
CGGCCAGCTGCTCTTCGCCAACTTCAACCAGGACAACACAGAAGTGAAAGGGGCATCAAG
AGCAGCTGGTCTTGGCCGTCGCGCTGTTGTCTGGTCCCTAGCTGTTGGTAGTAAGTCCGG
TTATAAATTTTTCTCCCTTTCTTCTGTGGATAAGCTGGAACAGATCTATGAATGCACCGA
TACGGAAGATGTGTGCATTGTAGAGAGATTGTTCTCCAGCAGCCTAGTGGCCATCGTGAG
CCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAAGGGAACTGAGATCTGCAA
CTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTGATAGTATG
CCTGGAGGAGTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAG
GGAGACGCCTCCAAACCCTGCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTA
CTTGGCGTACCCAGGGAGCGCGACCATCGGAGAGGTGCAGGTCTTCGATACCATTAATTT
GAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTTAGCGGCACTGGCCTTTGACGC
AAGTGGAACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGGGTATTTTCCAT
TCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCTG
CTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACTGAGACCGT
GCACATCTTCAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGAC
CGGGTACTTCGGGAAAGTGCTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGA
AATGTTCAACCAGGGCAGAGCCTTCGCCACGGTCCGCCTGCCATTCTGCGGCCACAAAAA
CATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGG
GTACCTGTACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCCCTGATGAAGCA
GCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACGA
CTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGGTACTTACGTGCCTTC
ATCCCCAACGAGACTTGCCTACACAGACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGA
GGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACAGCGAGCACCCGCCCATGATTCTTCG
GACTGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT