Restriction map for
pFN21AB8657
(Linear) (Six-base) MAPSORT of: KIBB8657.seq Check: 2430 from: 1 to: 1362
KIBB8657.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1055 1362
Size: 1055 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1362
Size: 750 612
AflIII A'CryG_T
Cuts at: 0 1064 1362
Size: 1064 298
AleI CACnn'nnGTG
Cuts at: 0 76 1362
Size: 76 1286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 766 798 1362
Size: 766 32 564
Fragments arranged by size:
766 564 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 1362
Size: 384 978
ApaLI G'TGCA_C
Cuts at: 0 814 1362
Size: 814 548
ApoI r'AATT_y
Cuts at: 0 160 1362
Size: 160 1202
AseI AT'TA_AT
Cuts at: 0 569 1362
Size: 569 793
AvaI C'yCGr_G
Cuts at: 0 829 1362
Size: 829 533
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1211 1244 1362
Size: 1211 33 118
Fragments arranged by size:
1211 118 33
BanI G'GyrC_C
Cuts at: 0 31 1042 1055 1282 1362
Size: 31 1011 13 227 80
Fragments arranged by size:
1011 227 80 31 13
BanII G_rGCy'C
Cuts at: 0 863 1362
Size: 863 499
BbeI G_GCGC'C
Cuts at: 0 35 1286 1362
Size: 35 1251 76
Fragments arranged by size:
1251 76 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 549 1362
Size: 549 813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 939 973 1252 1286 1362
Size: 939 34 279 34 76
Fragments arranged by size:
939 279 76 34 34
BglII A'GATC_T
Cuts at: 0 197 326 1151 1362
Size: 197 129 825 211
Fragments arranged by size:
825 211 197 129
Bme1580I G_kGCm'C
Cuts at: 0 818 1104 1362
Size: 818 286 258
Fragments arranged by size:
818 286 258
BmtI G_CTAG'C
Cuts at: 0 1009 1362
Size: 1009 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 418 780 1312 1362
Size: 234 184 362 532 50
Fragments arranged by size:
532 362 234 184 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 1362
Size: 802 560
BsaAI yAC'GTr
Cuts at: 0 1227 1362
Size: 1227 135
BsaHI Gr'CG_yC
Cuts at: 0 32 460 1283 1362
Size: 32 428 823 79
Fragments arranged by size:
823 428 79 32
BsaWI w'CCGG_w
Cuts at: 0 151 1362
Size: 151 1211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 328 358 394 424 1362
Size: 328 30 36 30 938
Fragments arranged by size:
938 328 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 743 871 1362
Size: 415 328 128 491
Fragments arranged by size:
491 415 328 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1362
Size: 571 791
BsiHKAI G_wGCw'C
Cuts at: 0 818 896 1337 1362
Size: 818 78 441 25
Fragments arranged by size:
818 441 78 25
BsmI GAATG_Cn'
Cuts at: 0 211 1362
Size: 211 1151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 451 1362
Size: 451 911
Bsp1286I G_dGCh'C
Cuts at: 0 818 863 896 1104 1337 1362
Size: 818 45 33 208 233 25
Fragments arranged by size:
818 233 208 45 33 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 923 1362
Size: 543 380 439
Fragments arranged by size:
543 439 380
BsrBI CCG'CTC
Cuts at: 0 20 1362
Size: 20 1342
BsrFI r'CCGG_y
Cuts at: 0 28 34 1118 1362
Size: 28 6 1084 244
Fragments arranged by size:
1084 244 28 6
BsrGI T'GTAC_A
Cuts at: 0 410 1061 1067 1362
Size: 410 651 6 295
Fragments arranged by size:
651 410 295 6
BstYI r'GATC_y
Cuts at: 0 197 326 1022 1151 1362
Size: 197 129 696 129 211
Fragments arranged by size:
696 211 197 129 129
BtgI C'CryG_G
Cuts at: 0 653 962 1027 1362
Size: 653 309 65 335
Fragments arranged by size:
653 335 309 65
EaeI y'GGCC_r
Cuts at: 0 36 108 263 984 1362
Size: 36 72 155 721 378
Fragments arranged by size:
721 378 155 72 36
EarI CTCTTCn'nnn_
Cuts at: 0 52 1362
Size: 52 1310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 780 958 1362
Size: 780 178 404
Fragments arranged by size:
780 404 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 418 750 780 1312 1362
Size: 234 184 332 30 532 50
Fragments arranged by size:
532 332 234 184 50 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1321 1362
Size: 1321 41
HaeII r_GCGC'y
Cuts at: 0 35 1286 1310 1362
Size: 35 1251 24 52
Fragments arranged by size:
1251 52 35 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 582 614 695 727 1150 1182 1362
Size: 582 32 81 32 423 32 180
Fragments arranged by size:
582 423 180 81 32 32 32
KasI G'GCGC_C
Cuts at: 0 31 1282 1362
Size: 31 1251 80
Fragments arranged by size:
1251 80 31
KpnI G_GTAC'C
Cuts at: 0 1059 1362
Size: 1059 303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1362
Size: 371 991
MscI TGG'CCA
Cuts at: 0 265 1362
Size: 265 1097
MslI CAynn'nnrTG
Cuts at: 0 76 436 1362
Size: 76 360 926
Fragments arranged by size:
926 360 76
MspA1I CmG'CkG
Cuts at: 0 42 100 1029 1207 1362
Size: 42 58 929 178 155
Fragments arranged by size:
929 178 155 58 42
NaeI GCC'GGC
Cuts at: 0 30 36 1362
Size: 30 6 1326
Fragments arranged by size:
1326 30 6
NarI GG'CG_CC
Cuts at: 0 32 1283 1362
Size: 32 1251 79
Fragments arranged by size:
1251 79 32
NgoMIV G'CCGG_C
Cuts at: 0 28 34 1362
Size: 28 6 1328
Fragments arranged by size:
1328 28 6
NheI G'CTAG_C
Cuts at: 0 1005 1362
Size: 1005 357
NspI r_CATG'y
Cuts at: 0 783 1068 1362
Size: 783 285 294
Fragments arranged by size:
783 294 285
PasI CC'CwG_GG
Cuts at: 0 526 1362
Size: 526 836
PciI A'CATG_T
Cuts at: 0 1064 1362
Size: 1064 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 869 1362
Size: 869 493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 1362
Size: 229 32 1101
Fragments arranged by size:
1101 229 32
PshAI GACnn'nnGTC
Cuts at: 0 1130 1362
Size: 1130 232
PsiI TTA'TAA
Cuts at: 0 158 1362
Size: 158 1204
PstI C_TGCA'G
Cuts at: 0 477 1212 1362
Size: 477 735 150
Fragments arranged by size:
735 477 150
PvuII CAG'CTG
Cuts at: 0 42 100 1207 1362
Size: 42 58 1107 155
Fragments arranged by size:
1107 155 58 42
RsrII CG'GwC_CG
Cuts at: 0 966 1362
Size: 966 396
SacII CC_GC'GG
Cuts at: 0 1030 1362
Size: 1030 332
SapI GCTCTTCn'nnn_
Cuts at: 0 52 1362
Size: 52 1310
SbfI CC_TGCA'GG
Cuts at: 0 477 1362
Size: 477 885
SfcI C'TryA_G
Cuts at: 0 336 473 1208 1362
Size: 336 137 735 154
Fragments arranged by size:
735 336 154 137
SfoI GGC'GCC
Cuts at: 0 33 1284 1362
Size: 33 1251 78
Fragments arranged by size:
1251 78 33
SmlI C'TyrA_G
Cuts at: 0 829 1362
Size: 829 533
StuI AGG'CCT
Cuts at: 0 479 1362
Size: 479 883
StyI C'CwwG_G
Cuts at: 0 286 1362
Size: 286 1076
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 1258 1267 1362
Size: 1030 228 9 95
Fragments arranged by size:
1030 228 95 9
TatI w'GTAC_w
Cuts at: 0 410 1061 1067 1362
Size: 410 651 6 295
Fragments arranged by size:
651 410 295 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 1362
Size: 211 32 1119
Fragments arranged by size:
1119 211 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 354 1152 1362
Size: 258 96 798 210
Fragments arranged by size:
798 258 210 96
XhoI C'TCGA_G
Cuts at: 0 829 1362
Size: 829 533
Enzymes that do cut:
Acc65I AcuI AflIII AleI AloI AlwNI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbeI BbsI BcgI
BglII Bme1580I BmtI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BstYI BtgI EaeI EarI EciI
Eco57MI EcoNI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PciI PflMI PpiI PshAI PsiI PstI PvuII RsrII
SacII SapI SbfI SfcI SfoI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI ApaI AscI AsiSI AvrII BamHI BbvCI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BplI Bpu10I
BpuEI BsaBI BseYI BsiEI BsiWI BspEI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NcoI NdeI NotI
NruI NsiI PacI PfoI PmeI PmlI PpuMI PspOMI
PspXI PsrI PvuI SacI SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XmaI XmnI ZraI