(Circular) (Six-base) MAPSORT of: pFN21AB8657.seq Check: 5410 from: 1 to: 6067 pFN21AB8657 6067 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3060 3388 3060 Size: 328 5739 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2755 4130 5514 5782 1632 Size: 1123 1375 1384 268 1917 Fragments arranged by size: 1917 1384 1375 1123 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 3069 4966 5943 1203 Size: 1866 1897 977 1327 Fragments arranged by size: 1897 1866 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 2081 1166 Size: 915 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 2771 Size: 32 6035 AlwNI CAG_nnn'CTG Cuts at: 2389 3810 5382 2389 Size: 1421 1572 3074 Fragments arranged by size: 3074 1572 1421 ApaLI G'TGCA_C Cuts at: 2819 4124 5280 2819 Size: 1305 1156 3606 Fragments arranged by size: 3606 1305 1156 ApoI r'AATT_y Cuts at: 1493 2165 3376 3602 3754 5863 1493 Size: 672 1211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 2574 4621 160 Size: 872 1542 2047 1606 Fragments arranged by size: 2047 1606 1542 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2834 3392 1870 Size: 73 891 558 4545 Fragments arranged by size: 4545 891 558 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3216 3249 3216 Size: 33 6034 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 3047 3060 3287 3388 Size: 325 217 627 249 1011 13 227 101 Cuts at: 3388 4843 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1011 627 325 249 227 217 101 13 BanII G_rGCy'C Cuts at: 1391 2868 3386 1391 Size: 1477 518 4072 Fragments arranged by size: 4072 1477 518 BbeI G_GCGC'C Cuts at: 2040 3291 2040 Size: 1251 4816 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2554 928 Size: 971 655 4441 Fragments arranged by size: 4441 971 655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2944 2978 3257 3291 4258 4292 1903 Size: 34 1007 34 279 34 967 34 3678 Fragments arranged by size: 3678 1007 967 279 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2202 2331 3156 6062 2202 Size: 129 825 2906 2207 Fragments arranged by size: 2906 2207 825 129 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2823 3109 4128 5284 1790 Size: 1033 286 1019 1156 2573 Fragments arranged by size: 2573 1156 1033 1019 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3014 1056 Size: 1958 4109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2239 2423 2785 3317 4727 1239 Size: 1000 184 362 532 1410 2579 Fragments arranged by size: 2579 1410 1000 532 362 184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 4730 882 Size: 617 1308 1923 2219 Fragments arranged by size: 2219 1923 1308 617 BsaAI yAC'GTr Cuts at: 493 1204 3232 3858 493 Size: 711 2028 626 2702 Fragments arranged by size: 2702 2028 711 626 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2465 3288 3874 Size: 53 83 186 936 504 428 823 586 Cuts at: 3874 4256 4891 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 823 635 586 504 428 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2156 4500 4898 5172 5319 5730 2156 Size: 2344 398 274 147 411 2493 Fragments arranged by size: 2493 2344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2333 2363 2399 2429 1178 Size: 30 165 30 930 30 36 30 4816 Fragments arranged by size: 4816 930 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2748 2876 1186 Size: 1234 328 128 4377 Fragments arranged by size: 4377 1234 328 128 BseYI C'CCAG_C Cuts at: 5270 5766 5270 Size: 496 5571 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 1163 Size: 573 840 4654 Fragments arranged by size: 4654 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2823 2901 3342 4128 4213 5284 2823 Size: 78 441 786 85 1071 3606 Fragments arranged by size: 3606 1071 786 441 85 78 BsmI GAATG_Cn' Cuts at: 2216 3578 3671 2216 Size: 1362 93 4612 Fragments arranged by size: 4612 1362 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2456 2456 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2823 2868 2901 3109 3342 3386 4128 Size: 399 1033 45 33 208 233 44 742 Cuts at: 4128 4213 5284 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1033 742 399 233 208 85 45 44 33 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2548 2928 3422 844 Size: 1704 380 494 3489 Fragments arranged by size: 3489 1704 494 380 BsrBI CCG'CTC Cuts at: 1394 1823 2025 3954 1394 Size: 429 202 1929 3507 Fragments arranged by size: 3507 1929 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2039 3123 4711 4898 1700 Size: 110 223 6 1084 1588 187 2869 Fragments arranged by size: 2869 1588 1084 223 187 110 6 BsrGI T'GTAC_A Cuts at: 96 2415 3066 3072 96 Size: 2319 651 6 3091 Fragments arranged by size: 3091 2319 651 6 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6067 BstYI r'GATC_y Cuts at: 1972 2202 2331 3027 3156 3397 3764 4149 4166 Size: 230 129 696 129 241 367 385 17 Cuts at: 4166 5618 5704 5716 6062 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 696 385 367 346 241 230 129 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2658 2967 3032 513 Size: 552 1593 309 65 3548 Fragments arranged by size: 3548 1593 552 309 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2113 2268 Size: 54 1425 50 275 18 211 72 155 Cuts at: 2268 2989 3514 4403 6032 8 Size: 721 525 889 1629 43 Fragments arranged by size: 1629 1425 889 721 525 275 211 155 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2057 3997 1353 Size: 704 1940 3423 Fragments arranged by size: 3423 1940 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2785 2963 4654 5028 5174 387 Size: 1064 138 1196 178 1691 374 146 1280 Fragments arranged by size: 1691 1280 1196 1064 374 178 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2239 2423 2755 2785 3317 4130 4727 Size: 393 607 184 332 30 532 813 597 Cuts at: 4727 5514 5782 1239 Size: 787 268 1524 Fragments arranged by size: 1524 813 787 607 597 532 393 332 268 184 30 EcoNI CCTnn'n_nnAGG Cuts at: 3326 3326 Size: 6067 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2040 3291 3315 5214 1678 Size: 362 1251 24 1899 2531 Fragments arranged by size: 2531 1899 1251 362 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2587 2619 2700 2732 3155 Size: 32 135 32 1015 32 81 32 423 Cuts at: 3155 3187 4708 4740 4782 4814 1373 Size: 32 1521 32 42 32 2626 Fragments arranged by size: 2626 1521 1015 423 135 81 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 2036 3287 2036 Size: 1251 4816 KpnI G_GTAC'C Cuts at: 3064 3392 3064 Size: 328 5739 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 5181 5365 1305 Size: 1071 2805 184 2007 Fragments arranged by size: 2805 2007 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 3807 Fragments arranged by size: 3807 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2441 4025 4384 4543 518 Size: 648 915 360 1584 359 159 2042 Fragments arranged by size: 2042 1584 915 648 360 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2105 3034 3212 3483 4160 5308 5553 Size: 441 58 929 178 271 677 1148 245 Cuts at: 5553 1606 Size: 2120 Fragments arranged by size: 2120 1148 929 677 441 271 245 178 58 NaeI GCC'GGC Cuts at: 2035 2041 2035 Size: 6 6061 NarI GG'CG_CC Cuts at: 2037 3288 2037 Size: 1251 4816 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 2033 2039 2033 Size: 6 6061 NheI G'CTAG_C Cuts at: 1052 3010 1052 Size: 1958 4109 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 2788 3073 3425 4970 1132 Size: 97 1559 285 352 1545 2229 Fragments arranged by size: 2229 1559 1545 352 285 97 PasI CC'CwG_GG Cuts at: 1348 2531 1348 Size: 1183 4884 PciI A'CATG_T Cuts at: 3069 4966 3069 Size: 1897 4170 PflMI CCAn_nnn'nTGG Cuts at: 1108 2874 1108 Size: 1766 4301 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2234 2266 4136 4168 5649 5681 2234 Size: 32 1870 32 1481 32 2620 Fragments arranged by size: 2620 1870 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3135 1613 Size: 1522 4545 PsiI TTA'TAA Cuts at: 2163 3637 2163 Size: 1474 4593 PstI C_TGCA'G Cuts at: 830 2482 3217 3419 830 Size: 1652 735 202 3478 Fragments arranged by size: 3478 1652 735 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2047 2105 3212 2047 Size: 58 1107 4902 Fragments arranged by size: 4902 1107 58 RsrII CG'GwC_CG Cuts at: 2971 2971 Size: 6067 SacII CC_GC'GG Cuts at: 3035 3035 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SapI GCTCTTCn'nnn_ Cuts at: 2057 2057 Size: 6067 SbfI CC_TGCA'GG Cuts at: 2482 3419 2482 Size: 937 5130 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2341 2478 3213 3415 4550 5231 5422 Size: 219 1296 137 735 202 1135 681 191 Cuts at: 5422 826 Size: 1471 Fragments arranged by size: 1471 1296 1135 735 681 219 202 191 137 SfoI GGC'GCC Cuts at: 2038 3289 2038 Size: 1251 4816 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2834 4171 5072 5334 820 Size: 197 926 891 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 2484 1517 Size: 967 5100 StyI C'CwwG_G Cuts at: 513 1065 2291 3506 513 Size: 552 1226 1215 3074 Fragments arranged by size: 3074 1226 1215 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3035 3263 3272 4094 4111 4264 4449 Size: 1255 1455 228 9 822 17 153 185 Cuts at: 4449 4894 325 Size: 445 1498 Fragments arranged by size: 1498 1455 1255 822 445 228 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 3066 3072 Size: 274 80 33 51 1443 438 651 6 Cuts at: 3072 4313 6025 96 Size: 1241 1712 138 Fragments arranged by size: 1712 1443 1241 651 438 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 6027 6059 2216 Size: 32 3779 32 2224 Fragments arranged by size: 3779 2224 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2359 3157 2263 Size: 96 798 5173 Fragments arranged by size: 5173 798 96 XhoI C'TCGA_G Cuts at: 1943 2834 1943 Size: 891 5176 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SanDI SexAI SfiI SrfI SwaI