Restriction map for
pFN21AB8657
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI MamI
MfeI MluI MroI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
SacI SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1055 GGTACC G^GTACC
AcsI 160 AAATTT [GA]^AATT[CT]
AflIII 1064 ACATGT A^C[GA][CT]GT
Alw44I 814 GTGCAC G^TGCAC
AlwI 1023 GATCC GGATC(4/5)
AlwNI 379 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 814 GTGCAC G^TGCAC
ApoI 160 AAATTT [GA]^AATT[CT]
AseI 568 ATTAAT AT^TAAT
AsnI 568 ATTAAT AT^TAAT
Asp718I 1055 GGTACC G^GTACC
AvaI 829 CTCGAG C^[CT]CG[GA]G
BalI 263 TGGCCA TGG^CCA
BanII 859 GAGCCC G[GA]GC[CT]^C
BbsI 556 GTCTTC GAAGAC(2/6)
BcnI 875 CCGGG CC^[GC]GG
BpuAI 556 GTCTTC GAAGAC(2/6)
BsaAI 1225 TACGTG [CT]AC^GT[GA]
BsaI 808 GAGACC GGTCTC(1/5)
BsaWI 151 TCCGGT [AT]^CCGG[AT]
BsgI 550 GTGCAG GTGCAG(16/14)
BsmBI 457 GAGACG CGTCTC(1/5)
BsmI 205 GAATGC GAATGC(1/-1)
BsoBI 829 CTCGAG C^[CT]CG[GA]G
BspLU11I 1064 ACATGT A^CATGT
BsrBI 18 GAGCGG CCGCTC(-3/-3)
BsrI 621 ACTGG ACTGG(1/-1)
CpoI 965 CGGTCCG CG^G[AT]CCG
EarI 46 CTCTTC CTCTTC(1/4)
EcoNI 1317 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 286 CCAAGG C^C[AT][AT]GG
KpnI 1055 GGTACC GGTAC^C
Ksp632I 46 CTCTTC CTCTTC(1/4)
KspI 1027 CCGCGG CCGC^GG
MaeII 1226 ACGT A^CGT
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
NciI 875 CCGGG CC^[GC]GG
NheI 1005 GCTAGC G^CTAGC
PaeR7I 829 CTCGAG C^TCGAG
PflMI 863 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1126 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 568 ATTAAT AT^TAAT
RsrII 965 CGGTCCG CG^G[AT]CCG
SacII 1027 CCGCGG CCGC^GG
SapI 45 GCTCTTC GCTCTTC(1/4)
SbfI 472 CCTGCAGG CCTGCA^GG
SmlI 829 CTCGAG C^T[CT][GA]AG
Sse8387I 472 CCTGCAGG CCTGCA^GG
StuI 477 AGGCCT AGG^CCT
StyI 286 CCAAGG C^C[AT][AT]GG
Van91I 863 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 829 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbeI 31 GGCGCC GGCGC^C
1282 GGCGCC
BcgI 1263 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
950 GCAGAGCCTTCG
BspMI 914 ACCTGC ACCTGC(4/8)
552 GCAGGT
HgaI 459 GACGC GACGC(5/10)
631 GACGC
KasI 31 GGCGCC G^GCGCC
1282 GGCGCC
MaeIII 928 GTGAC ^GT[ACGT]AC
1186 GTCAC
MslI 72 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CATGAAGGTG
NaeI 28 GCCGGC GCC^GGC
34 GCCGGC
NarI 31 GGCGCC GG^CGCC
1282 GGCGCC
NgoMIV 28 GCCGGC G^CCGGC
34 GCCGGC
NspI 779 GCATGT [GA]CATG^[CT]
1064 ACATGT
PleI 1159 GACTC GAGTC(4/5)
403 GAGTC
PstI 473 CTGCAG CTGCA^G
1208 CTGCAG
SfaNI 88 GCATC GCATC(5/9)
749 GCATC
SfoI 31 GGCGCC GGC^GCC
1282 GGCGCC
TaqI 560 TCGA T^CGA
830 TCGA
Tsp45I 928 GTGAC ^GT[GC]AC
1186 GTCAC
TspRI 620 CACTG CA[GC]TG[ACGT][ACGT]^
804 CACTG
TthHB8I 560 TCGA T^CGA
830 TCGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BbvI BfaI
BglII BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrFI BsrGI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SspBI TfiI Tru9I TseI
Tsp509I XcmI XhoII