GLIS1 ORF clone pFN21AB7846

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB7846

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Clone info.

Species

human

Clone Name

pFN21AB7846 Sequence (with vector) Restriction Map

Product ID

FHC20314

ORF Sequence

DNA sequence (1860 bp)
Protein sequence (620 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB7846

Symbol

GLIS1

Description

GLIS family zinc finger 1

Original Clone ID

cs00346

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1860 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 620 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS582.1 9e-103 100.0 GLIS1 CCDS6451.1 1.3e-27 41.6 GLIS3 CCDS43784.1 1.5e-27 41.6 GLIS3 CCDS33283.1 1.2e-13 33.1 GLI2 CCDS53806.1 1.4e-13 32.9 GLI1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMSmart IPR015880 195 220 SM00355 Zinc finger IPR015880 229 256 SM00355 Zinc finger IPR015880 262 286 SM00355 Zinc finger IPR015880 292 316 SM00355 Zinc finger IPR015880 322 346 SM00355 Zinc finger ProfileScan IPR007087 229 261 PS50157 Zinc finger IPR007087 262 291 PS50157 Zinc finger IPR007087 292 321 PS50157 Zinc finger IPR007087 322 351 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_671726 620 610378 100.0 100.0 XP_016855899 795 610378 100.0 100.0 XP_016855897 795 610378 100.0 100.0 XP_016855898 791 610378 99.4 100.0 XP_005251445 708 610192,610199 41.6 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7846 Download

>KIBB7846  1860 bp
ATGGCAGAGGCCCGCACATCCCTGTCTGCCCACTGTCGGGGCCCGCTGGCCACTGGCCTG
CACCCAGACCTGGACCTCCCGGGCCGAAGCCTCGCCACCCCTGCGCCTTCCTGCTACCTT
CTGGGCAGCGAACCCAGCTCTGGCCTGGGCCTCCAGCCCGAGACCCACCTCCCCGAGGGC
AGCCTGAAGCGGTGCTGCGTCTTGGGCCTACCCCCCACCTCCCCAGCCTCCTCCTCACCC
TGTGCCTCCTCCGACGTCACCTCCATCATCCGCTCCTCCCAGACGTCTCTGGTCACCTGT
GTAAATGGACTCCGGAGCCCCCCTCTGACGGGAGATCTGGGGGGCCCTTCCAAGCGGGCC
CGGCCTGGCCCTGCATCGACGGACAGCCATGAGGGCAGCTTGCAACTTGAAGCCTGCCGG
AAGGCGAGCTTCCTGAAGCAGGAACCCGCGGATGAGTTTTCAGAGCTCTTTGGGCCTCAC
CAGCAGGGCCTGCCGCCCCCCTATCCCCTGTCTCAGTTGCCGCCTGGCCCAAGCCTTGGA
GGCCTGGGGCTGGGCCTGGCAGGCAGGGTGGTGGCCGGGCGGCAGGCGTGCCGCTGGGTG
GACTGCTGTGCAGCCTATGAGCAGCAGGAGGAGCTGGTGCGGCACATCGAGAAGAGCCAC
ATCGACCAGCGCAAGGGCGAGGACTTCACCTGCTTCTGGGCTGGCTGCGTGCGCCGCTAC
AAGCCCTTCAACGCCCGCTACAAGCTGCTCATCCACATGCGAGTGCACTCGGGCGAGAAG
CCCAACAAGTGCATGTTTGAAGGCTGCAGCAAGGCCTTCTCACGGCTGGAGAACCTCAAG
ATCCACCTGAGGAGCCACACGGGCGAGAAGCCGTACCTGTGCCAGCACCCGGGTTGCCAG
AAGGCCTTCAGCAACTCCAGCGACCGCGCCAAGCACCAGCGCACCCACCTAGACACGAAG
CCGTACGCCTGTCAGATCCCTGGCTGCTCCAAGCGCTACACAGACCCCAGCTCCCTCCGC
AAGCACGTCAAGGCCCATTCAGCCAAAGAGCAGCAGGTGCGTAAGAAGCTGCATGCGGGC
CCTGACACCGAGGCCGACGTCCTGACCGAGTGTCTGGTCCTGCAGCAGCTCCACACGTCC
ACACAGCTGGCTGCCAGCGACGGCAAGGGTGGCTGTGGCCTGGGCCAGGAGCTGCTCCCA
GGTGTGTATCCTGGCTCCATCACCCCCCATAACGGACTTGCATCGGGCCTCCTGCCCCCA
GCGCACGACGTACCTTCCAGGCACCACCCGCTGGATGCCACCACCAGTTCCCACCACCAT
CTGTCCCCTCTGCCCATGGCTGAGAGCACCCGGGATGGGTTGGGGCCCGGCCTCCTCTCA
CCAATAGTCAGCCCCCTGAAGGGGCTGGGGCCACCGCCGCTGCCCCCATCCTCTCAGAGC
CATTCTCCGGGGGGCCAGCCCTTCCCCACACTCCCCAGCAAGCCGTCCTACCCACCCTTC
CAGAGCCCTCCACCCCCGCCTCTGCCCAGCCCACAAGGTTACCAGGGCAGTTTCCACTCC
ATCCAGAGTTGCTTCCCCTATGGCGACTGCTACCGGATGGCTGAACCAGCAGCCGGTGGG
GACGGACTGGTCGGGGAGACCCACGGTTTCAACCCCCTGCGGCCCAATGGCTACCACAGC
CTCAGCACGCCCTTGCCTGCCACAGGCTATGAGGCCCTGGCTGAGGCCTCATGCCCCACA
GCGCTGCCACAGCAGCCATCTGAAGATGTGGTGTCCAGCGGCCCCGAGGACTGTGGCTTC
TTCCCCAATGGAGCCTTTGACCACTGCCTGGGCCACATCCCCTCCATCTACACAGACACC

ORF Protein

Cloned ORF protein sequence for pFN21AB7846 Download

>KIBB7846  620 aa
MAEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEG
SLKRCCVLGLPPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRA
RPGPASTDSHEGSLQLEACRKASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLG
GLGLGLAGRVVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRY
KPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQ
KAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHAG
PDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPP
AHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQS
HSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGG
DGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGF
FPNGAFDHCLGHIPSIYTDT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7846 Download

>pFN21AB7846  6565 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGAGGCCCGCACATCCCTGTCTGCCCACTG
TCGGGGCCCGCTGGCCACTGGCCTGCACCCAGACCTGGACCTCCCGGGCCGAAGCCTCGC
CACCCCTGCGCCTTCCTGCTACCTTCTGGGCAGCGAACCCAGCTCTGGCCTGGGCCTCCA
GCCCGAGACCCACCTCCCCGAGGGCAGCCTGAAGCGGTGCTGCGTCTTGGGCCTACCCCC
CACCTCCCCAGCCTCCTCCTCACCCTGTGCCTCCTCCGACGTCACCTCCATCATCCGCTC
CTCCCAGACGTCTCTGGTCACCTGTGTAAATGGACTCCGGAGCCCCCCTCTGACGGGAGA
TCTGGGGGGCCCTTCCAAGCGGGCCCGGCCTGGCCCTGCATCGACGGACAGCCATGAGGG
CAGCTTGCAACTTGAAGCCTGCCGGAAGGCGAGCTTCCTGAAGCAGGAACCCGCGGATGA
GTTTTCAGAGCTCTTTGGGCCTCACCAGCAGGGCCTGCCGCCCCCCTATCCCCTGTCTCA
GTTGCCGCCTGGCCCAAGCCTTGGAGGCCTGGGGCTGGGCCTGGCAGGCAGGGTGGTGGC
CGGGCGGCAGGCGTGCCGCTGGGTGGACTGCTGTGCAGCCTATGAGCAGCAGGAGGAGCT
GGTGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAGGACTTCACCTGCTT
CTGGGCTGGCTGCGTGCGCCGCTACAAGCCCTTCAACGCCCGCTACAAGCTGCTCATCCA
CATGCGAGTGCACTCGGGCGAGAAGCCCAACAAGTGCATGTTTGAAGGCTGCAGCAAGGC
CTTCTCACGGCTGGAGAACCTCAAGATCCACCTGAGGAGCCACACGGGCGAGAAGCCGTA
CCTGTGCCAGCACCCGGGTTGCCAGAAGGCCTTCAGCAACTCCAGCGACCGCGCCAAGCA
CCAGCGCACCCACCTAGACACGAAGCCGTACGCCTGTCAGATCCCTGGCTGCTCCAAGCG
CTACACAGACCCCAGCTCCCTCCGCAAGCACGTCAAGGCCCATTCAGCCAAAGAGCAGCA
GGTGCGTAAGAAGCTGCATGCGGGCCCTGACACCGAGGCCGACGTCCTGACCGAGTGTCT
GGTCCTGCAGCAGCTCCACACGTCCACACAGCTGGCTGCCAGCGACGGCAAGGGTGGCTG
TGGCCTGGGCCAGGAGCTGCTCCCAGGTGTGTATCCTGGCTCCATCACCCCCCATAACGG
ACTTGCATCGGGCCTCCTGCCCCCAGCGCACGACGTACCTTCCAGGCACCACCCGCTGGA
TGCCACCACCAGTTCCCACCACCATCTGTCCCCTCTGCCCATGGCTGAGAGCACCCGGGA
TGGGTTGGGGCCCGGCCTCCTCTCACCAATAGTCAGCCCCCTGAAGGGGCTGGGGCCACC
GCCGCTGCCCCCATCCTCTCAGAGCCATTCTCCGGGGGGCCAGCCCTTCCCCACACTCCC
CAGCAAGCCGTCCTACCCACCCTTCCAGAGCCCTCCACCCCCGCCTCTGCCCAGCCCACA
AGGTTACCAGGGCAGTTTCCACTCCATCCAGAGTTGCTTCCCCTATGGCGACTGCTACCG
GATGGCTGAACCAGCAGCCGGTGGGGACGGACTGGTCGGGGAGACCCACGGTTTCAACCC
CCTGCGGCCCAATGGCTACCACAGCCTCAGCACGCCCTTGCCTGCCACAGGCTATGAGGC
CCTGGCTGAGGCCTCATGCCCCACAGCGCTGCCACAGCAGCCATCTGAAGATGTGGTGTC
CAGCGGCCCCGAGGACTGTGGCTTCTTCCCCAATGGAGCCTTTGACCACTGCCTGGGCCA
CATCCCCTCCATCTACACAGACACCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT