Restriction map for
pFN21AB7846
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 311 TCCGGA T^CCGGA
AflIII 1133 ACACGT A^C[GA][CT]GT
AhdI 1104 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 763 GTGCAC G^TGCAC
AocI 846 CCTGAGG CC^T[ACGT]AGG
ApaLI 763 GTGCAC G^TGCAC
Asp700I 900 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1104 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 47 TGGCCA TGG^CCA
BanI 1280 GGCACC G^G[CT][GA]CC
BciVI 1206 GTATCC GTATCC(6/5)
BfaI 949 CTAG C^TAG
BglII 333 AGATCT A^GATCT
BseAI 311 TCCGGA T^CCGGA
BsiEI 921 CGACCG CG[GA][CT]^CG
BsiWI 962 CGTACG C^GTACG
BsmBI 284 CGTCTC CGTCTC(1/5)
BspEI 311 TCCGGA T^CCGGA
BsrBI 270 CCGCTC CCGCTC(-3/-3)
BsrFI 1612 GCCGGT [GA]^CCGG[CT]
BstXI 1139 CCACACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 846 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1612 GCCGGT [GA]^CCGG[CT]
DraIII 236 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1621 GACGGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1104 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 651 GAAGAG CTCTTC(1/4)
Eco81I 846 CCTGAGG CC^T[ACGT]AGG
HgaI 197 GCGTC GACGC(5/10)
HinfI 308 GACTC G^A[ACGT]TC
Ksp632I 651 GAAGAG CTCTTC(1/4)
KspI 446 CCGCGG CCGC^GG
MaeI 949 CTAG C^TAG
MluNI 47 TGGCCA TGG^CCA
MroI 311 TCCGGA T^CCGGA
MscI 47 TGGCCA TGG^CCA
NcoI 1334 CCATGG C^CATGG
PleI 308 GACTC GAGTC(4/5)
PvuII 1144 CAGCTG CAG^CTG
SacI 463 GAGCTC GAGCT^C
SacII 446 CCGCGG CCGC^GG
SapI 651 GAAGAGC GCTCTTC(1/4)
SmlI 835 CTCAAG C^T[CT][GA]AG
SphI 1071 GCATGC GCATG^C
XcmI 134 CCAGCTCTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 900 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 447 CGCG CG^CG
925 CGCG
AlwI 840 GATCC GGATC(4/5)
975 GATCC
AlwNI 484 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1186 CAGGAGCTG
Aor51HI 992 AGCGCT AGC^GCT
1740 AGCGCT
AvaII 72 GGACC G^G[AT]CC
1116 GGTCC
BbvCI 1680 CCTCAGC CCTCAGC(-5/-2)
1720 GCTGAGG
BsaI 160 GAGACC GGTCTC(1/5)
1636 GAGACC
BsaWI 311 TCCGGA [AT]^CCGG[AT]
1592 ACCGGA
BsgI 58 CTGCAC GTGCAG(16/14)
608 GTGCAG
BsmFI 1619 GGGAC GGGAC(10/14)
1323 GTCCC
BspMI 688 ACCTGC ACCTGC(4/8)
1053 GCAGGT
BstAPI 483 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1050 GCAGCAGGTGC
BstEII 291 GGTCACC G^GT[ACGT]ACC
1537 GGTTACC
BstPI 291 GGTCACC G^GT[ACGT]ACC
1537 GGTTACC
BstUI 447 CGCG CG^CG
925 CGCG
EaeI 47 TGGCCA [CT]^GGCC[GA]
572 TGGCCG
Eco47III 992 AGCGCT AGC^GCT
1740 AGCGCT
EcoO65I 291 GGTCACC G^GT[ACGT]ACC
1537 GGTTACC
EcoT14I 534 CCTTGG C^C[AT][AT]GG
1334 CCATGG
HaeII 992 AGCGCT [GA]GCGC^[CT]
1740 AGCGCT
MslI 750 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATGCGAGTG
MvnI 447 CGCG CG^CG
925 CGCG
PstI 804 CTGCAG CTGCA^G
1120 CTGCAG
SfcI 804 CTGCAG C^T[GA][CT]AG
1120 CTGCAG
StyI 534 CCTTGG C^C[AT][AT]GG
1334 CCATGG
Tsp45I 256 GTCAC ^GT[GC]AC
292 GTCAC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
ApaI AspHI AvaI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI StuI TaqI TseI TspRI
TthHB8I Van91I XhoII XmaCI XmaI