(Circular) (Six-base) MAPSORT of: pFN21AB7846.seq Check: 9409 from: 1 to: 6565 pFN21AB7846 6565 bp With 182 enzymes: SgfI * November 30, 2011 11:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 3049 2702 Size: 347 6218 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2262 2291 3105 4375 Size: 53 83 186 936 726 29 814 1270 Cuts at: 4375 278 Size: 2468 Fragments arranged by size: 2468 1270 936 814 726 186 83 53 29 AccI GT'mk_AC Cuts at: 1937 3908 1937 Size: 1971 4594 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2459 2896 3422 3786 4628 6012 6280 Size: 578 249 437 526 364 842 1384 268 Cuts at: 6280 1632 Size: 1917 Fragments arranged by size: 1917 1384 842 578 526 437 364 268 249 AfeI AGC'GCT Cuts at: 2999 3747 2999 Size: 748 5817 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 3138 5464 6441 1203 Size: 1935 2326 977 1327 Fragments arranged by size: 2326 1935 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 3114 5294 3114 Size: 2180 4385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6565 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3713 1184 Size: 2529 4036 AlwNI CAG_nnn'CTG Cuts at: 2494 3196 4308 5880 2494 Size: 702 1112 1572 3179 Fragments arranged by size: 3179 1572 1112 702 ApaI G_GGCC'C Cuts at: 2048 2351 2365 3086 3372 2048 Size: 303 14 721 286 5241 Fragments arranged by size: 5241 721 303 286 14 ApaLI G'TGCA_C Cuts at: 2768 4622 5778 2768 Size: 1854 1156 3555 Fragments arranged by size: 3555 1854 1156 ApoI r'AATT_y Cuts at: 1493 3874 4100 4252 6361 1493 Size: 2381 226 152 2109 1697 Fragments arranged by size: 2381 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 2083 2162 2177 2773 2893 3354 3788 Size: 73 140 79 15 596 120 461 434 Cuts at: 3788 3890 1870 Size: 102 4545 Fragments arranged by size: 4545 596 461 434 140 120 102 79 73 15 BamHI G'GATC_C Cuts at: 3895 4262 3895 Size: 367 6198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3285 3886 5341 618 Size: 325 217 627 1498 601 1455 1842 Fragments arranged by size: 1842 1498 1455 627 601 325 217 BanII G_rGCy'C Cuts at: 1391 2048 2324 2351 2365 2472 3086 3372 3512 Size: 657 276 27 14 107 614 286 140 Cuts at: 3512 3884 1391 Size: 372 4072 Fragments arranged by size: 4072 657 614 372 286 276 140 107 27 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5594 BbvCI CC'TCA_GC Cuts at: 3686 3726 3686 Size: 40 6525 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4756 4790 1903 Size: 34 2819 34 3678 Fragments arranged by size: 3678 2819 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3222 4457 5667 3222 Size: 1235 1210 4120 Fragments arranged by size: 4120 1235 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2397 2544 2565 Size: 107 122 71 631 605 725 147 21 Cuts at: 2565 2865 5176 136 Size: 300 2311 1525 Fragments arranged by size: 2311 1525 725 631 605 300 147 122 107 71 21 BglII A'GATC_T Cuts at: 2338 6560 2338 Size: 4222 2343 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 2048 2351 2365 2772 3086 3372 4626 5782 Size: 258 303 14 407 314 286 1254 1156 Cuts at: 5782 1790 Size: 2573 Fragments arranged by size: 2573 1254 1156 407 314 303 286 258 14 BmgBI CAC'GTC Cuts at: 1318 3031 3141 1318 Size: 1713 110 4742 Fragments arranged by size: 4742 1713 110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4296 5254 442 Size: 416 53 83 399 24 2879 958 1753 Fragments arranged by size: 2879 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2343 3465 2343 Size: 1122 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2852 2905 5225 1239 Size: 902 711 53 2320 2579 Fragments arranged by size: 2579 2320 902 711 53 Bpu10I CC'TnA_GC Cuts at: 3686 3726 3686 Size: 40 6525 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 4690 5555 5853 2825 Size: 1865 865 298 3537 Fragments arranged by size: 3537 1865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 3635 5228 882 Size: 617 660 1476 1593 2219 Fragments arranged by size: 2219 1593 1476 660 617 BsaAI yAC'GTr Cuts at: 493 1204 4356 493 Size: 711 3152 2702 Fragments arranged by size: 3152 2702 711 BsaBI GATnn'nnATC Cuts at: 6559 6559 Size: 6565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 2288 3102 4372 Size: 53 83 186 936 726 29 814 1270 Cuts at: 4372 4754 5389 275 Size: 382 635 1451 Fragments arranged by size: 1451 1270 936 814 726 635 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 2316 3597 4998 5396 5670 5817 6228 2316 Size: 1281 1401 398 274 147 411 2653 Fragments arranged by size: 2653 1401 1281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2249 2279 2999 3029 3830 Size: 30 165 30 846 30 720 30 801 Cuts at: 3830 3860 1178 Size: 30 3883 Fragments arranged by size: 3883 846 801 720 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 2227 2242 2269 2648 2869 3368 1186 Size: 1038 3 15 27 379 221 499 4383 Fragments arranged by size: 4383 1038 499 379 221 27 15 3 BseYI C'CCAG_C Cuts at: 2138 2227 2554 2598 3011 3262 3409 3479 3530 Size: 89 327 44 413 251 147 70 51 Cuts at: 3530 5768 6264 2138 Size: 2238 496 2439 Fragments arranged by size: 2439 2238 496 413 327 251 147 89 70 51 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 2634 1163 Size: 573 312 586 5094 Fragments arranged by size: 5094 586 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 4015 4776 5804 1540 Size: 275 186 928 1086 761 1028 2301 Fragments arranged by size: 2301 1086 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2472 2772 3353 4626 4711 5782 2472 Size: 300 581 1273 85 1071 3255 Fragments arranged by size: 3255 1273 1071 581 300 85 BsiWI C'GTAC_G Cuts at: 2967 2967 Size: 6565 BsmI GAATG_Cn' Cuts at: 4076 4169 4076 Size: 93 6472 BsmBI CGTCTCn'nnnn_ Cuts at: 2295 2295 Size: 6565 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2324 2351 2365 2472 2772 3086 Size: 399 258 276 27 14 107 300 314 Cuts at: 3086 3353 3372 3512 3884 4626 4711 5782 1391 Size: 267 19 140 372 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 314 300 276 267 258 140 107 85 27 19 14 BspEI T'CCGG_A Cuts at: 2316 6228 2316 Size: 3912 2653 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 3049 3920 844 Size: 1858 347 871 3489 Fragments arranged by size: 3489 1858 871 347 BsrBI CCG'CTC Cuts at: 1394 1823 2277 4452 1394 Size: 429 454 2175 3507 Fragments arranged by size: 3507 2175 454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5060 5234 65 Size: 1183 3812 174 1396 Fragments arranged by size: 3812 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3617 5209 5396 1700 Size: 110 1807 1592 187 2869 Fragments arranged by size: 2869 1807 1592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 4625 5637 4625 Size: 1012 5553 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 3061 794 Size: 1700 567 4298 Fragments arranged by size: 4298 1700 567 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 2296 3542 1184 Size: 1112 1246 4207 Fragments arranged by size: 4207 1246 1112 BstXI CCAn_nnnn'nTGG Cuts at: 3151 3151 Size: 6565 BstYI r'GATC_y Cuts at: 1972 2338 2844 2979 3895 4262 4647 4664 6116 Size: 366 506 135 916 367 385 17 1452 Cuts at: 6116 6202 6214 6560 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 916 506 385 367 366 346 135 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2852 2852 Size: 6565 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 3339 3646 513 Size: 552 939 447 888 307 3432 Fragments arranged by size: 3432 939 888 552 447 307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5826 Fragments arranged by size: 5826 683 56 BtsI GCAGTG_nn' Cuts at: 3826 4081 4875 4895 3826 Size: 255 794 20 5496 Fragments arranged by size: 5496 794 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6530 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DraIII CAC_nnn'GTG Cuts at: 2246 2246 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3632 5572 809 Size: 507 2316 1940 1802 Fragments arranged by size: 2316 1940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2577 4012 Size: 54 1425 50 275 18 222 525 1435 Cuts at: 4012 4901 6530 8 Size: 889 1629 43 Fragments arranged by size: 1629 1435 1425 889 525 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 4495 1353 Size: 1298 1844 3423 Fragments arranged by size: 3423 1844 1298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5152 5526 5672 387 Size: 1064 138 3563 374 146 1280 Fragments arranged by size: 3563 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2470 2470 Size: 6565 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2210 2459 2852 2896 2905 3422 Size: 393 509 69 249 393 44 9 517 Cuts at: 3422 3786 4628 5225 6012 6280 1239 Size: 364 842 597 787 268 1524 Fragments arranged by size: 1524 842 787 597 517 509 393 393 364 268 249 69 44 9 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2441 3188 3705 3725 2177 Size: 264 747 517 20 5017 Fragments arranged by size: 5017 747 517 264 20 EcoO109I rG'GnC_Cy Cuts at: 2044 2347 2348 2491 3083 3368 3718 2044 Size: 303 1 143 592 285 350 4891 Fragments arranged by size: 4891 592 350 303 285 143 1 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 6565 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 3001 3749 5712 1678 Size: 1323 748 1963 2531 Fragments arranged by size: 2531 1963 1323 748 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2323 2355 2999 Size: 32 135 32 677 32 42 32 644 Cuts at: 2999 3031 3100 3132 3761 3793 5206 5238 5280 Size: 32 69 32 629 32 1413 32 42 Cuts at: 5280 5312 1373 Size: 32 2626 Fragments arranged by size: 2626 1413 677 644 629 135 69 42 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3909 4155 669 Size: 988 281 1971 246 3079 Fragments arranged by size: 3079 1971 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6565 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 5679 5863 1305 Size: 975 3399 184 2007 Fragments arranged by size: 3399 2007 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 10 Size: 54 1425 343 222 4521 Fragments arranged by size: 4521 1425 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 2765 4523 4882 5041 518 Size: 648 1593 6 1758 359 159 2042 Fragments arranged by size: 2042 1758 1593 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2050 2453 2598 3151 3295 3424 3783 3981 Size: 444 403 145 553 144 129 359 198 Cuts at: 3981 4658 5806 6051 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 553 444 403 359 245 198 145 144 129 NcoI C'CATG_G Cuts at: 513 1065 2004 3339 513 Size: 552 939 1335 3739 Fragments arranged by size: 3739 1335 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 2764 2800 3080 3923 5468 1132 Size: 97 1535 36 280 843 1545 2229 Fragments arranged by size: 2229 1545 1535 843 280 97 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6565 PciI A'CATG_T Cuts at: 5464 5464 Size: 6565 PflMI CCAn_nnn'nTGG Cuts at: 1108 2540 3295 3832 1108 Size: 1432 755 537 3841 Fragments arranged by size: 3841 1432 755 537 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PspOMI G'GGCC_C Cuts at: 2044 2347 2361 3082 3368 2044 Size: 303 14 721 286 5241 Fragments arranged by size: 5241 721 303 286 14 PstI C_TGCA'G Cuts at: 830 2813 3129 3917 830 Size: 1983 316 788 3478 Fragments arranged by size: 3478 1983 788 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 3151 3151 Size: 6565 SacI G_AGCT'C Cuts at: 2472 2472 Size: 6565 SacII CC_GC'GG Cuts at: 2454 2454 Size: 6565 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SapI GCTCTTCn'nnn_ Cuts at: 2651 2651 Size: 6565 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 4813 6525 4813 Size: 1712 4853 SfcI C'TryA_G Cuts at: 826 1045 2809 3125 3913 5048 5729 5920 826 Size: 219 1764 316 788 1135 681 191 1471 Fragments arranged by size: 1764 1471 1135 788 681 316 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 2085 2895 3356 3892 1872 Size: 213 810 461 536 4545 Fragments arranged by size: 4545 810 536 461 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 4669 5570 5832 820 Size: 197 926 897 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 897 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3080 3923 1132 Size: 1948 843 3774 Fragments arranged by size: 3774 1948 843 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 2547 2819 2909 3731 1517 Size: 1030 272 90 822 4351 Fragments arranged by size: 4351 1030 822 272 90 StyI C'CwwG_G Cuts at: 513 1065 2004 2539 3339 4004 513 Size: 552 939 535 800 665 3074 Fragments arranged by size: 3074 939 800 665 552 535 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2082 2590 2963 3125 4592 4609 4762 Size: 1255 502 508 373 162 1467 17 153 Cuts at: 4762 4947 5392 325 Size: 185 445 1498 Fragments arranged by size: 1498 1467 1255 508 502 445 373 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4811 6523 96 Size: 274 80 33 51 1443 2834 1712 138 Fragments arranged by size: 2834 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3311 3343 3830 3862 6525 6557 3311 Size: 32 487 32 2663 32 3319 Fragments arranged by size: 3319 2663 487 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 2146 Size: 6565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 2083 2893 3354 3890 1870 Size: 213 810 461 536 4545 Fragments arranged by size: 4545 810 536 461 213 XmnI GAAnn'nnTTC Cuts at: 2909 4694 2909 Size: 1785 4780 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2260 2289 3103 4373 Size: 53 83 186 936 726 29 814 1270 Cuts at: 4373 276 Size: 2468 Fragments arranged by size: 2468 1270 936 814 726 186 83 53 29 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI