(Linear) (Six-base) MAPSORT of: KIBB7846.seq Check: 9642 from: 1 to: 1860 KIBB7846.orf With 182 enzymes: SgfI * November 30, 2011 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 697 1044 1860 Size: 697 347 816 Fragments arranged by size: 816 697 347 AatII G_ACGT'C Cuts at: 0 257 286 1100 1860 Size: 257 29 814 760 Fragments arranged by size: 814 760 257 29 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 454 891 1417 1781 1860 Size: 205 249 437 526 364 79 Fragments arranged by size: 526 437 364 249 205 79 AfeI AGC'GCT Cuts at: 0 994 1742 1860 Size: 994 748 118 Fragments arranged by size: 994 748 118 AflIII A'CryG_T Cuts at: 0 1133 1860 Size: 1133 727 AhdI GACnn_n'nnGTC Cuts at: 0 1109 1860 Size: 1109 751 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1708 1860 Size: 1708 152 AlwNI CAG_nnn'CTG Cuts at: 0 489 1191 1860 Size: 489 702 669 Fragments arranged by size: 702 669 489 ApaI G_GGCC'C Cuts at: 0 43 346 360 1081 1367 1860 Size: 43 303 14 721 286 493 Fragments arranged by size: 721 493 303 286 43 14 ApaLI G'TGCA_C Cuts at: 0 763 1860 Size: 763 1097 AvaI C'yCGr_G Cuts at: 0 78 157 172 768 888 1349 1783 1860 Size: 78 79 15 596 120 461 434 77 Fragments arranged by size: 596 461 434 120 79 78 77 15 BanI G'GyrC_C Cuts at: 0 1280 1860 Size: 1280 580 BanII G_rGCy'C Cuts at: 0 43 319 346 360 467 1081 1367 1507 Size: 43 276 27 14 107 614 286 140 Cuts at: 1507 1860 Size: 353 Fragments arranged by size: 614 353 286 276 140 107 43 27 14 BbvCI CC'TCA_GC Cuts at: 0 1681 1721 1860 Size: 1681 40 139 Fragments arranged by size: 1681 139 40 BciVI GTATCCnnnnn_n' Cuts at: 0 1217 1860 Size: 1217 643 BglI GCCn_nnn'nGGC Cuts at: 0 392 539 560 860 1860 Size: 392 147 21 300 1000 Fragments arranged by size: 1000 392 300 147 21 BglII A'GATC_T Cuts at: 0 333 1860 Size: 333 1527 Bme1580I G_kGCm'C Cuts at: 0 43 346 360 767 1081 1367 1860 Size: 43 303 14 407 314 286 493 Fragments arranged by size: 493 407 314 303 286 43 14 BmgBI CAC'GTC Cuts at: 0 1026 1136 1860 Size: 1026 110 724 Fragments arranged by size: 1026 724 110 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 338 1460 1860 Size: 338 1122 400 Fragments arranged by size: 1122 400 338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 847 900 1860 Size: 136 711 53 960 Fragments arranged by size: 960 711 136 53 Bpu10I CC'TnA_GC Cuts at: 0 1681 1721 1860 Size: 1681 40 139 Fragments arranged by size: 1681 139 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 1860 Size: 820 1040 BsaI GGTCTCn'nnnn_ Cuts at: 0 154 1630 1860 Size: 154 1476 230 Fragments arranged by size: 1476 230 154 BsaHI Gr'CG_yC Cuts at: 0 254 283 1097 1860 Size: 254 29 814 763 Fragments arranged by size: 814 763 254 29 BsaWI w'CCGG_w Cuts at: 0 311 1592 1860 Size: 311 1281 268 Fragments arranged by size: 1281 311 268 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 244 274 994 1024 1825 1855 1860 Size: 244 30 720 30 801 30 5 Fragments arranged by size: 801 720 244 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 219 222 237 264 643 864 1363 1860 Size: 219 3 15 27 379 221 499 497 Fragments arranged by size: 499 497 379 221 219 27 15 3 BseYI C'CCAG_C Cuts at: 0 133 222 549 593 1006 1257 1404 1474 Size: 133 89 327 44 413 251 147 70 Cuts at: 1474 1525 1860 Size: 51 335 Fragments arranged by size: 413 335 327 251 147 133 89 70 51 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 629 1860 Size: 43 586 1231 Fragments arranged by size: 1231 586 43 BsiEI CG_ry'CG Cuts at: 0 924 1860 Size: 924 936 BsiHKAI G_wGCw'C Cuts at: 0 467 767 1348 1860 Size: 467 300 581 512 Fragments arranged by size: 581 512 467 300 BsiWI C'GTAC_G Cuts at: 0 962 1860 Size: 962 898 BsmBI CGTCTCn'nnnn_ Cuts at: 0 290 1860 Size: 290 1570 Bsp1286I G_dGCh'C Cuts at: 0 43 319 346 360 467 767 1081 1348 Size: 43 276 27 14 107 300 314 267 Cuts at: 1348 1367 1507 1860 Size: 19 140 353 Fragments arranged by size: 353 314 300 276 267 140 107 43 27 19 14 BspEI T'CCGG_A Cuts at: 0 311 1860 Size: 311 1549 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 697 1044 1860 Size: 697 347 816 Fragments arranged by size: 816 697 347 BsrBI CCG'CTC Cuts at: 0 272 1860 Size: 272 1588 BsrFI r'CCGG_y Cuts at: 0 1612 1860 Size: 1612 248 BstAPI GCAn_nnn'nTGC Cuts at: 0 489 1056 1860 Size: 489 567 804 Fragments arranged by size: 804 567 489 BstEII G'GTnAC_C Cuts at: 0 291 1537 1860 Size: 291 1246 323 Fragments arranged by size: 1246 323 291 BstXI CCAn_nnnn'nTGG Cuts at: 0 1146 1860 Size: 1146 714 BstYI r'GATC_y Cuts at: 0 333 839 974 1860 Size: 333 506 135 886 Fragments arranged by size: 886 506 333 135 Bsu36I CC'TnA_GG Cuts at: 0 847 1860 Size: 847 1013 BtgI C'CryG_G Cuts at: 0 446 1334 1641 1860 Size: 446 888 307 219 Fragments arranged by size: 888 446 307 219 BtsI GCAGTG_nn' Cuts at: 0 1821 1860 Size: 1821 39 DraIII CAC_nnn'GTG Cuts at: 0 241 1860 Size: 241 1619 DrdI GACnn_nn'nnGTC Cuts at: 0 1627 1860 Size: 1627 233 EaeI y'GGCC_r Cuts at: 0 47 572 1860 Size: 47 525 1288 Fragments arranged by size: 1288 525 47 EarI CTCTTCn'nnn_ Cuts at: 0 646 1860 Size: 646 1214 EcoICRI GAG'CTC Cuts at: 0 465 1860 Size: 465 1395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 205 454 847 891 900 1417 1781 Size: 136 69 249 393 44 9 517 364 Cuts at: 1781 1860 Size: 79 Fragments arranged by size: 517 393 364 249 136 79 69 44 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 172 436 1183 1700 1720 1860 Size: 172 264 747 517 20 140 Fragments arranged by size: 747 517 264 172 140 20 EcoO109I rG'GnC_Cy Cuts at: 0 39 342 343 486 1078 1363 1713 1860 Size: 39 303 1 143 592 285 350 147 Fragments arranged by size: 592 350 303 285 147 143 39 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 444 1860 Size: 444 1416 HaeII r_GCGC'y Cuts at: 0 996 1744 1860 Size: 996 748 116 Fragments arranged by size: 996 748 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 244 276 318 350 994 1026 1095 1127 Size: 244 32 42 32 644 32 69 32 Cuts at: 1127 1756 1788 1860 Size: 629 32 72 Fragments arranged by size: 644 629 244 72 69 42 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 275 1860 Size: 275 1585 MscI TGG'CCA Cuts at: 0 49 1860 Size: 49 1811 MslI CAynn'nnrTG Cuts at: 0 754 760 1860 Size: 754 6 1100 Fragments arranged by size: 1100 754 6 MspA1I CmG'CkG Cuts at: 0 45 448 593 1146 1290 1419 1778 1860 Size: 45 403 145 553 144 129 359 82 Fragments arranged by size: 553 403 359 145 144 129 82 45 NcoI C'CATG_G Cuts at: 0 1334 1860 Size: 1334 526 NspI r_CATG'y Cuts at: 0 759 795 1075 1860 Size: 759 36 280 785 Fragments arranged by size: 785 759 280 36 PflMI CCAn_nnn'nTGG Cuts at: 0 535 1290 1827 1860 Size: 535 755 537 33 Fragments arranged by size: 755 537 535 33 PspOMI G'GGCC_C Cuts at: 0 39 342 356 1077 1363 1860 Size: 39 303 14 721 286 497 Fragments arranged by size: 721 497 303 286 39 14 PstI C_TGCA'G Cuts at: 0 808 1124 1860 Size: 808 316 736 Fragments arranged by size: 808 736 316 PvuII CAG'CTG Cuts at: 0 1146 1860 Size: 1146 714 SacI G_AGCT'C Cuts at: 0 467 1860 Size: 467 1393 SacII CC_GC'GG Cuts at: 0 449 1860 Size: 449 1411 SapI GCTCTTCn'nnn_ Cuts at: 0 646 1860 Size: 646 1214 SfcI C'TryA_G Cuts at: 0 804 1120 1860 Size: 804 316 740 Fragments arranged by size: 804 740 316 SmaI CCC'GGG Cuts at: 0 80 890 1351 1860 Size: 80 810 461 509 Fragments arranged by size: 810 509 461 80 SmlI C'TyrA_G Cuts at: 0 835 1860 Size: 835 1025 SphI G_CATG'C Cuts at: 0 1075 1860 Size: 1075 785 StuI AGG'CCT Cuts at: 0 542 814 904 1726 1860 Size: 542 272 90 822 134 Fragments arranged by size: 822 542 272 134 90 StyI C'CwwG_G Cuts at: 0 534 1334 1860 Size: 534 800 526 Fragments arranged by size: 800 534 526 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 77 585 958 1120 1860 Size: 77 508 373 162 740 Fragments arranged by size: 740 508 373 162 77 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1306 1338 1825 1857 1860 Size: 1306 32 487 32 3 Fragments arranged by size: 1306 487 32 32 3 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 141 1860 Size: 141 1719 XmaI C'CCGG_G Cuts at: 0 78 888 1349 1860 Size: 78 810 461 511 Fragments arranged by size: 810 511 461 78 XmnI GAAnn'nnTTC Cuts at: 0 904 1860 Size: 904 956 ZraI GAC'GTC Cuts at: 0 255 284 1098 1860 Size: 255 29 814 762 Fragments arranged by size: 814 762 255 29 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbvCI BciVI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I NcoI NspI PflMI PspOMI PstI PvuII SacI SacII SapI SfcI SmaI SmlI SphI StuI StyI TaqII TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI BlpI BmrI BmtI BsaAI BsaBI BsmI BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI