CERS3 ORF clone pFN21AB7656

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Halotag ORF Clone pFN21AB7656

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Clone info.

Species

human

Clone Name

pFN21AB7656 Sequence (with vector) Restriction Map

Product ID

FHC01940

Flexi Clone PID

FXC01940

ORF Sequence

DNA sequence (1182 bp)
Protein sequence (394 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527465]

Gene

KIBB7656

Symbol

CERS3

Alias : ARCI9, LASS3

Description

ceramide synthase 3, transcript variant 1

Original Clone ID

cs00156

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1182 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 394 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10384.1 2.7e-132 99.7 CERS3 CCDS973.1 1.4e-68 52.6 CERS2 CCDS12197.1 1.3e-55 46.4 CERS4 CCDS8801.1 7.7e-49 42.4 CERS5 CCDS2228.1 1.3e-47 41.5 CERS6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001356 92 136 PF00046 Homeobox IPR006634 142 335 PF03798 TRAM/LAG1/CLN8 homology domain HMMSmart IPR001356 84 142 SM00389 Homeobox IPR006634 141 342 SM00724 TRAM/LAG1/CLN8 homology domain ProfileScan IPR001356 94 138 PS50071 Homeobox IPR006634 141 342 PS50922 TRAM/LAG1/CLN8 homology domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 146 ACWRFAFYLMITVAGIAFLYDKP 168 PRIMARY 23 2 215 DFLAHIIHHLAAISLMSFSWCAN 237 SECONDARY 23 3 276 FIFSTIFFISRLIVFPFWILYCT 298 PRIMARY 23 4 307 EPFFSYIFLNLQLMILQVLHLYW 329 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011519659 394 615023,615276 99.7 100.0 XP_011519660 394 615023,615276 99.7 100.0 XP_016877491 394 615023,615276 99.7 100.0 XP_011519657 394 615023,615276 99.7 100.0 NP_001277270 394 615023,615276 99.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7656 Download

>KIBB7656  1182 bp
ATGCTTCACACCACTGCCAGAAGAGCTGCCAGAATGTTTTGGACGTTTAAAGAATGGTTC
TGGTTGGAAAGATTCTGGCTTCCTCCAACAATAAAGTGGTCAGATCTTGAGGATCACGAT
GGACTCGTCTTTGTAAAACCTTCTCATTTATACGTGACAATTCCATATGCTTTTCTCTTG
CTGATTATCAGGCGTGTATTTGAAAAATTTGTTGCTTCACCTCTAGCAAAATCATTTGGC
ATTAAAGAGACAGTTCGAAAGGTTACACCAAATACTGTCTTAGAGAATTTTTTCAAACAT
TCCACAAGGCAACCATTGCAAACTGATATTTATGGACTGGCAAAGAAGTGTAACTTGACG
GAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGGCGGAATCAAGAGAGGCCTTCCAGGCTG
AAGAAATTCCAGGAAGCTTGCTGGAGATTTGCATTTTACTTAATGATCACTGTTGCTGGA
ATTGCGTTTCTTTATGATAAACCTTGGCTATATGACTTATGGGAGGTTTGGAATGGCTAT
CCCAAACAGCCCCTGCTGCCATCCCAGTACTGGTACTACATTTTAGAAATGAGTTTTTAT
TGGTCTCTGTTATTTAGACTTGGCTTTGATGTCAAGAGAAAGGATTTTCTAGCTCATATC
ATCCACCACCTGGCTGCTATTAGTCTGATGAGCTTCTCTTGGTGTGCTAATTATATTCGC
AGTGGGACCCTCGTGATGATTGTACACGATGTGGCTGACATTTGGCTGGAGTCTGCTAAG
ATGTTTTCTTATGCTGGATGGACGCAGACCTGTAACACCCTGTTTTTCATCTTCTCCACC
ATATTTTTCATCAGCCGCCTCATTGTTTTTCCTTTCTGGATTTTATATTGCACGCTGATC
TTGCCTATGTATCACCTCGAGCCTTTCTTTTCATACATCTTCCTCAACCTACAGCTCATG
ATCTTGCAGGTTCTTCACCTTTACTGGGGTTATTACATCTTGAAGATGCTCAACAGATGT
ATATTCATGAAGAGCATCCAGGATGTGAGGAGTGATGACGAGGATTATGAAGAGGAAGAG
GAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAAGAGATGGATTGTTTAAAGAACGGCCTC
GGGGCTGAGAGGCACCTCATTCCCAATGGCCAGCATGGCCAT

ORF Protein

Cloned ORF protein sequence for pFN21AB7656 Download

>KIBB7656  394 aa
MLHTTARRAARMFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLL
LIIRRVFEKFVASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLT
ERQVERWFRSRRNQERPSRLKKFQEACWRFAFYLMITVAGIAFLYDKPWLYDLWEVWNGY
PKQPLLPSQYWYYILEMSFYWSLLFRLGFDVKRKDFLAHIIHHLAAISLMSFSWCANYIR
SGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRLIVFPFWILYCTLI
LPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSIQDVRSDDEDYEEEE
EEEEEEATKGKEMDCLKNGLGAERHLIPNGQHGH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7656 Download

>pFN21AB7656  5887 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTTCACACCACTGCCAGAAGAGCTGCCAGAAT
GTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATAAA
GTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATACGT
GACAATTCCATATGCTTTTCTCTTGCTGATTATCAGGCGTGTATTTGAAAAATTTGTTGC
TTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAATAC
TGTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTATGG
ACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGGCG
GAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCATT
TTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATATGA
CTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGGTA
CTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTCAA
GAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGCTT
CTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTGGC
TGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGTAA
CACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCTTT
CTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCATA
CATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTTCTTCACCTTTACTGGGGTTATTA
CATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGTGA
TGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAAGA
GATGGATTGTTTAAAGAACGGCCTCGGGGCTGAGAGGCACCTCATTCCCAATGGCCAGCA
TGGCCATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT