Restriction map for
pFN21AB7656
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HapII
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwI 111 GGATC GGATC(4/5)
AlwNI 547 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 725 GGACC G^G[AT]CC
BanI 1151 GGCACC G^G[CT][GA]CC
BclI 464 TGATCA T^GATCA
BglII 102 AGATCT A^GATCT
BsaAI 151 TACGTG [CT]AC^GT[GA]
BsaI 602 GGTCTC GGTCTC(1/5)
BseRI 1047 GAGGAG GAGGAG(10/8)
BsmFI 724 GGGAC GGGAC(10/14)
Bsp1407I 741 TGTACA T^GTACA
BspMI 966 GCAGGT ACCTGC(4/8)
BsrDI 314 CATTGC GCAATG(2/0)
BsrGI 741 TGTACA T^GTACA
BssSI 730 CTCGTG CACGAG(-5/-1)
BstAPI 309 GCAACCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 254 TTCGAA TT^CGAA
BstYI 102 AGATCT [GA]^GATC[CT]
CfoI 363 GCGC GCG^C
Cfr13I 725 GGACC G^G[ACGT]CC
DraII 724 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 745 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 724 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 502 CCTTGG C^C[AT][AT]GG
FbaI 464 TGATCA T^GATCA
HaeII 362 AGCGCC [GA]GCGC^[CT]
HgaI 801 GACGC GACGC(5/10)
HhaI 363 GCGC GCG^C
HinP1I 363 GCGC G^CGC
HindIII 434 AAGCTT A^AGCTT
MflI 102 AGATCT [GA]^GATC[CT]
NdeI 164 CATATG CA^TATG
NspV 254 TTCGAA TT^CGAA
PaeR7I 916 CTCGAG C^TCGAG
PflMI 663 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 724 GGGACCC [GA]G^G[AT]CC[CT]
Sau96I 725 GGACC G^G[ACGT]CC
ScaI 566 AGTACT AGT^ACT
SfcI 949 CTACAG C^T[GA][CT]AG
SfiI 1168 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 254 TTCGAA TT^CGAA
SspBI 741 TGTACA T^GTACA
StuI 406 AGGCCT AGG^CCT
StyI 502 CCTTGG C^C[AT][AT]GG
Tsp45I 154 GTGAC ^GT[GC]AC
Van91I 663 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 916 CTCGAG C^TCGAG
XhoII 102 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 855 CCGC CCGC(-3/-1)
393 GCGG
Asp700I 285 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
420 GAAGAAATTC
AvaI 916 CTCGAG C^[CT]CG[GA]G
1138 CTCGGG
BalI 1167 TGGCCA TGG^CCA
1176 TGGCCA
BfaI 223 CTAG C^TAG
649 CTAG
BglI 408 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1169 GCCAGCATGGC
BpmI 441 CTGGAG CTGGAG(16/14)
766 CTGGAG
BsaJI 502 CCTTGG C^C[ACGT][ACGT]GG
1137 CCTCGG
BsmAI 247 GAGAC GTCTC(1/5)
603 GTCTC
BsoBI 916 CTCGAG C^[CT]CG[GA]G
1138 CTCGGG
BspHI 956 TCATGA T^CATGA
1025 TCATGA
DraI 46 TTTAAA TTT^AAA
1125 TTTAAA
EaeI 1167 TGGCCA [CT]^GGCC[GA]
1176 TGGCCA
MaeI 223 CTAG C^TAG
649 CTAG
MaeII 43 ACGT A^CGT
152 ACGT
MluNI 1167 TGGCCA TGG^CCA
1176 TGGCCA
MscI 1167 TGGCCA TGG^CCA
1176 TGGCCA
RcaI 956 TCATGA T^CATGA
1025 TCATGA
SapI 20 GAAGAGC GCTCTTC(1/4)
1029 GAAGAGC
SfaNI 1005 GATGC GCATC(5/9)
1034 GCATC
SmlI 106 CTTGAG C^T[CT][GA]AG
916 CTCGAG
TaqI 255 TCGA T^CGA
917 TCGA
TfiI 71 GATTC G^A[AT]TC
396 GAATC
TthHB8I 255 TCGA T^CGA
917 TCGA
XcmI 85 CCAACAATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
559 CCATCCCAGTACTGG
XmnI 285 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
420 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI Tru9I TseI Tsp509I
TspRI