(Circular) (Six-base) MAPSORT of: pFN21AB7656.seq Check: 6432 from: 1 to: 5887 pFN21AB7656 5887 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 3950 5334 5602 1632 Size: 812 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 812 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 2557 3630 5202 2557 Size: 1073 1572 3242 Fragments arranged by size: 3242 1572 1073 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2210 2290 2429 3196 3422 3574 5683 1493 Size: 717 80 139 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 717 226 152 139 80 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2921 3143 3212 1870 Size: 73 978 222 69 4545 Fragments arranged by size: 4545 978 222 73 69 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3156 3208 4663 618 Size: 325 217 627 1369 52 1455 1842 Fragments arranged by size: 1842 1455 1369 627 325 217 52 BanII G_rGCy'C Cuts at: 1391 3206 1391 Size: 1815 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4078 4112 1903 Size: 34 50 34 2057 34 3678 Fragments arranged by size: 3678 2057 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 2469 1548 Size: 921 4966 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2419 3180 4498 Size: 107 122 71 631 605 747 761 1318 Cuts at: 4498 136 Size: 1525 Fragments arranged by size: 1525 1318 761 747 631 605 122 107 71 BglII A'GATC_T Cuts at: 2107 5882 2107 Size: 3775 2112 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 3948 5104 1790 Size: 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 2998 3618 Size: 416 53 83 399 24 1146 435 620 Cuts at: 3618 4576 442 Size: 958 1753 Fragments arranged by size: 1753 1146 958 620 435 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2467 2792 4547 1239 Size: 1228 325 1755 2579 Fragments arranged by size: 2579 1755 1228 325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 4012 4877 5175 2132 Size: 1880 865 298 2844 Fragments arranged by size: 2844 1880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2613 4550 882 Size: 617 1114 1937 2219 Fragments arranged by size: 2219 1937 1114 617 BsaAI yAC'GTr Cuts at: 493 1204 2158 3678 493 Size: 711 954 1520 2702 Fragments arranged by size: 2702 1520 954 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3067 1186 Size: 1881 4006 BseYI C'CCAG_C Cuts at: 5090 5586 5090 Size: 496 5391 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3206 3948 4033 5104 1391 Size: 399 1416 742 85 1071 2174 Fragments arranged by size: 2174 1416 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 2961 3030 3776 5506 2961 Size: 69 746 1730 3342 Fragments arranged by size: 3342 1730 746 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2962 3242 844 Size: 2118 280 3489 Fragments arranged by size: 3489 2118 280 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2318 4382 4556 65 Size: 1183 1070 2064 174 1396 Fragments arranged by size: 2064 1396 1183 1070 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 2746 96 Size: 2650 3237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 2735 3947 4959 2735 Size: 1212 1012 3663 Fragments arranged by size: 3663 1212 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2320 794 Size: 1526 4361 BstBI TT'CG_AA Cuts at: 2260 4691 2260 Size: 2431 3456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2107 3217 3584 3969 3986 5438 5524 5536 Size: 135 1110 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5335 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2016 2731 3403 4197 4217 2016 Size: 715 672 794 20 3686 Fragments arranged by size: 3686 794 715 672 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 2053 3132 3191 2053 Size: 1079 59 4749 Fragments arranged by size: 4749 1079 59 DraIII CAC_nnn'GTG Cuts at: 2755 2755 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3172 3181 3334 Size: 54 1425 50 275 18 1342 9 153 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1342 889 275 153 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 3029 3069 3075 3081 3093 3817 1353 Size: 667 1009 40 6 6 12 724 3423 Fragments arranged by size: 3423 1009 724 667 40 12 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2413 4474 4848 4994 387 Size: 1064 138 824 2061 374 146 1280 Fragments arranged by size: 2061 1280 1064 824 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2467 2792 3950 4547 5334 5602 Size: 393 812 23 325 1158 597 787 268 Cuts at: 5602 1239 Size: 1524 Fragments arranged by size: 1524 1158 812 787 597 393 325 268 23 EcoO109I rG'GnC_Cy Cuts at: 2730 2730 Size: 5887 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5887 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2371 5034 1678 Size: 693 2663 2531 Fragments arranged by size: 2663 2531 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373 Size: 32 135 32 2956 32 42 32 2626 Fragments arranged by size: 2956 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 2439 748 Size: 1691 4196 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 2114 5001 5185 1305 Size: 744 65 2887 184 2007 Fragments arranged by size: 2887 2007 744 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 3174 3183 10 Size: 54 1425 343 1342 9 2714 Fragments arranged by size: 2714 1425 1342 343 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 3183 3845 4204 4363 518 Size: 648 2017 662 359 159 2042 Fragments arranged by size: 2042 2017 662 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3303 3980 5128 5373 1606 Size: 1697 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5335 NdeI CA'TA_TG Cuts at: 387 2170 387 Size: 1783 4104 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 3245 4790 1132 Size: 97 2016 1545 2229 Fragments arranged by size: 2229 2016 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 1108 Size: 1566 4321 PfoI T'CCnGG_A Cuts at: 2433 3042 2433 Size: 609 5278 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2730 2730 Size: 5887 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PspXI vC'TCGA_Gb Cuts at: 2921 2921 Size: 5887 PstI C_TGCA'G Cuts at: 830 3239 830 Size: 2409 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SanDI GG'GwC_CC Cuts at: 2730 2730 Size: 5887 SapI GCTCTTCn'nnn_ Cuts at: 2020 3029 2020 Size: 1009 4878 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 2573 4135 5847 2573 Size: 1562 1712 2613 Fragments arranged by size: 2613 1712 1562 SfcI C'TryA_G Cuts at: 826 1045 2954 3235 4370 5051 5242 826 Size: 219 1909 281 1135 681 191 1471 Fragments arranged by size: 1909 1471 1135 681 281 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3180 3180 Size: 5887 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 2921 3991 4892 5154 820 Size: 197 926 168 810 1070 901 262 1553 Fragments arranged by size: 1553 1070 926 901 810 262 197 168 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2413 1517 Size: 896 4991 StyI C'CwwG_G Cuts at: 513 1065 2507 3326 513 Size: 552 1442 819 3074 Fragments arranged by size: 3074 1442 819 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3914 3931 4084 4269 4714 325 Size: 1255 2334 17 153 185 445 1498 Fragments arranged by size: 2334 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2571 2746 4133 Size: 274 80 33 51 1443 594 175 1387 Cuts at: 4133 5845 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1387 594 274 175 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2097 2571 2097 Size: 474 5413 XhoI C'TCGA_G Cuts at: 1943 2921 1943 Size: 978 4909 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2294 2429 4016 2294 Size: 135 1587 4165 Fragments arranged by size: 4165 1587 135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SexAI SfoI SrfI SwaI