EVPL ORF clone pFN21AB3462

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB3462

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Clone info.

Species

human

Clone Name

pFN21AB3462 Sequence (with vector) Restriction Map

Product ID

FHC26196

Flexi Clone PID

FXC26196

ORF Sequence

DNA sequence (6099 bp)
Protein sequence (2033 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB3462

Symbol

EVPL

Alias : EVPK

Description

envoplakin

Original Clone ID

cp01547

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 6099 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 2033 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11737.1 0 100.0 EVPL CCDS82207.1 0 98.9 EVPL CCDS10526.1 4.8e-54 30.8 PPL CCDS45626.1 2.3e-29 70.9 EVPLL CCDS47936.1 4.3e-18 24.9 PLEC The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001101 1681 1719 PF00681 Plectin repeat IPR001101 1828 1862 PF00681 Plectin repeat IPR001101 1856 1900 PF00681 Plectin repeat IPR001101 1894 1929 PF00681 Plectin repeat IPR001101 1933 1975 PF00681 Plectin repeat HMMSmart IPR018159 229 330 SM00150 Spectrin/alpha-actinin IPR001101 1185 1226 SM00250 Plectin repeat IPR001101 1678 1713 SM00250 Plectin repeat IPR001101 1729 1763 SM00250 Plectin repeat IPR001101 1818 1855 SM00250 Plectin repeat IPR001101 1856 1893 SM00250 Plectin repeat IPR001101 1894 1931 SM00250 Plectin repeat IPR001101 1932 1969 SM00250 Plectin repeat IPR001101 1970 2007 SM00250 Plectin repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001979 2033 601590 100.0 100.0 NP_001307676 2055 601590 98.9 100.0 XP_016878864 1772 602871 30.5 86.7 XP_016878863 1785 602871 30.7 86.0 XP_006720965 1769 602871 31.0 86.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3462 Download

>KIBB3462  6099 bp
ATGTTCAAGGGGCTGAGCAAAGGCTCCCAGGGGAAGGGGTCCCCCAAGGGCTCCCCCGCC
AAGGGGTCCCCCAAAGGCTCCCCCAGCAGGCACAGCCGGGCTGCCACCCAGGAGCTGGCC
CTTCTCATCTCCCGCATGCAAGCCAACGCCGACCAGGTGGAGCGGGACATCCTGGAGACG
CAGAAGAGGCTGCAGCAGGACCGGCTGAACAGTGAGCAGAGCCAGGCCCTGCAGCACCAG
CAGGAGACGGGCCGCAGCCTGAAGGAGGCTGAGGTGCTGCTCAAGGACCTCTTCCTGGAC
GTGGACAAGGCCCGGCGGCTCAAGCACCCGCAGGCTGAGGAGATTGAGAAGGACATCAAG
CAGCTGCACGAGCGGGTGACCCAGGAGTGTGCGGAGTACCGTGCCCTGTACGAGAAGATG
GTGCTGCCCCCCGACGTGGGACCCAGGGTCGACTGGGCACGCGTGCTGGAGCAGAAACAG
AAGCAGGTCTGCGCAGGCCAGTACGGGCCGGGCATGGCGGAGCTGGAGCAACAGATCGCC
GAGCACAACATCCTGCAGAAGGAGATCGACGCCTATGGGCAGCAGCTGCGGAGCCTCGTG
GGGCCGGATGCAGCCACCATCCGGAGCCAATACCGAGACCTACTGAAGGCGGCGTCGTGG
CGTGGGCAGAGCCTGGGCAGCCTGTACACGCACCTCCAGGGCTGCACGCGGCAGCTGAGC
GCCCTGGCTGAGCAGCAGCGCCGCATCCTGCAGCAGGACTGGAGCGACCTCATGGCCGAC
CCTGCGGGCGTGCGGCGGGAGTACGAGCACTTCAAGCAGCACGAGCTGCTGAGTCAGGAG
CAGAGCGTGAACCAGCTGGAGGACGACGGCGAGCGCATGGTGGAGCTGCGGCACCCCGCG
GTGGGGCCCATCCAGGCCCACCAGGAGGCCCTGAAGATGGAGTGGCAGAACTTCCTGAAC
CTGTGTATCTGCCAGGAGACCCAGCTGCAGCACGTGGAGGACTACCGCCGGTTCCAGGAA
GAGGCCGACTCAGTCAGCCAGACCCTGGCGAAGCTCAACTCCAACTTGGATGCCAAGTAC
AGCCCTGCACCTGGGGGCCCCCCTGGCGCCCCCACAGAGCTGCTGCAACAGCTGGAGGCA
GAGGAAAAACGGCTGGCCGTCACCGAGAGGGCCACTGGGGACCTGCAGCGGCGAAGCCGG
GATGTGGCCCCTCTGCCACAGCGAAGAAACCCCCCTCAGCAGCCCCTGCACGTGGACAGC
ATCTGCGACTGGGACTCAGGAGAAGTGCAGCTGCTGCAGGGTGAGCGGTATAAGCTGGTA
GATAACACTGACCCGCACGCCTGGGTCGTGCAGGGCCCTGGCGGGGAGACCAAGCGTGCT
CCCGCCGCCTGCTTCTGCATCCCAGCACCAGACCCTGATGCTGTGGCCAGGGCCTCCCGG
CTGGCCTCAGAGCTGCAGGCCCTGAAGCAGAAATTGGCCACAGTCCAGAGCCGCCTGAAG
GCCAGTGCTGTGGAGTCTCTTCGGCCCAGCCAGCAGGCTCCATCTGGCTCAGACCTGGCC
AACCCACAGGCCCAGAAGCTCCTGACACAGATGACCCGGCTGGATGGAGACCTGGGACAG
ATAGAGAGGCAGGTGCTGGCCTGGGCGCGGGCCCCGCTGAGCCGCCCCACACCCTTGGAG
GACTTGGAGGGCCGCATCCACAGCCATGAGGGCACAGCCCAGCGCCTGCAGAGCCTGGGA
ACGGAGAAGGAGACAGCCCAGAAGGAGTGCGAGGCGTTTCTGTCCACGCGGCCCGTGGGC
CCCGCTGCCCTGCAGCTGCCCGTAGCCCTCAACAGCGTGAAGAACAAGTTCAGTGACGTG
CAGGTTCTGTGCAGCCTCTACGGGGAGAAAGCCAAGGCTGCCCTGGATCTGGAGCGGCAG
ATCCAGGATGCGGACAGGGTCATCCGAGGCTTCGAGGCCACCCTGGTGCAGGAGGCCCCC
ATCCCTGCTGAACCGGGGGCTCTGCAGGAGAGGGTCAGCGAGCTGCAGCGCCAGCGGAGG
GAGCTGCTGGAACAGCAGACCTGCGTGCTGCGGCTACACCGCGCGCTGAAGGCCTCGGAG
CACGCATGCGCTGCCCTGCAGAACAACTTCCAGGAGTTCTGCCAAGACCTGCCTCGCCAG
CAGCGCCAGGTGCGAGCCCTCACCGACCGCTACCACGCCGTAGGGGACCAGCTGGACCTG
CGGGAGAAGGTGGTGCAGGATGCCGCCCTCACCTACCAGCAGTTCAAGAACTGCAAGGAT
AACCTGAGCTCCTGGCTGGAGCACCTGCCCCGCAGCCAGGTGCGGCCCAGCGACGGCCCC
AGCCAGATCGCCTACAAGCTGCAGGCGCAGAAGAGGCTGACGCAGGAGATCCAGAGCCGA
GAGCGGGACAGGGCCACAGCATCCCACCTCTCCCAGGCCCTGCAGGCAGCGCTCCAGGAC
TATGAGCTCCAGGCAGACACCTACCGCTGCTCTTTGGAGCCCACCCTGGCAGTGTCAGCC
CCCAAGAGACCCCGAGTGGCTCCCCTGCAAGAGAGCATCCAAGCCCAGGAGAAGAACCTT
GCAAAGGCCTATACTGAGGTTGCAGCAGCACAGCAGCAGCTGCTCCAGCAGCTGGAGTTT
GCTAGAAAAATGCTGGAGAAGAAGGAGCTCAGTGAGGACATCCGAAGGACCCATGATGCA
AAGCAGGGCTCCGAGAGCCCTGCCCAAGCAGGGAGAGAGTCAGAGGCCCTGAAGGCCCAG
CTGGAAGAGGAGAGGAAGCGGGTGGCCCGGGTGCAGCATGAGCTGGAGGCGCAGAGGAGC
CAACTGCTGCAGCTGAGGACCCAGCGGCCCTTGGAGAGGCTGGAGGAGAAGGAAGTGGTA
GAGTTCTACCGGGACCCCCAGCTGGAGGGCAGCCTGTCCAGGGTGAAGGCCCAGGTGGAG
GAGGAGGGCAAGCGGCGGGCTGGCCTGCAGGCAGACCTGGAAGTGGCAGCCCAGAAGGTC
GTGCAGCTGGAAAGCAAGAGGAAGACCATGCAGCCTCATCTGCTGACCAAGGAGGTCACC
CAGGTGGAGAGGGACCCCGGCCTGGACAGCCAGGCGGCCCAGCTCAGGATCCAGATCCAG
CAGCTCCGCGGGGAGGATGCCGTCATCTCGGCCCGGCTGGAAGGGCTGAAGAAGGAGCTA
CTGGCCCTTGAGAAGAGGGAGGTGGACGTGAAGGAGAAGGTCGTGGTGAAAGAGGTAGTC
AAGGTGGAGAAGAATCTGGAAATGGTCAAGGCAGCCCAGGCTCTGAGGCTGCAGATGGAG
GAGGATGCTGCGCGGAGGAAGCAGGCGGAGGAGGCTGTGGCCAAGCTACAGGCTCGCATC
GAAGACCTGGAGCGGGCTATCAGCTCGGTGGAGCCCAAGGTCATCGTGAAGGAGGTGAAG
AAGGTGGAGCAGGACCCAGGGCTCCTCCAGGAGTCCTCCAGGCTGAGGAGCCTCCTCGAG
GAGGAGAGGACCAAGAACGCGACGCTGGCCAGGGAGCTGAGCGACCTGCACAGCAAGTAC
AGCGTGGTGGAGAAGCAGAGGCCCAAAGTGCAGCTCCAGGAGCGCGTCCACGAGATCTTC
CAGGTGGATCCGGAGACAGAGCAGGAGATCACTCGGCTCAAGGCCAAGCTGCAGGAGATG
GCGGGCAAGAGGAGCGGTGTGGAGAAGGAGGTGGAGAAGCTGCTGCCCGACCTGGAGGTC
CTGCGGGCCCAGAAGCCCACGGTGGAGTACAAGGAGGTGACCCAGGAGGTGGTGAGGCAT
GAGAGGAGCCCCGAGGTGCTGCGTGAGATCGACCGCCTGAAGGCTCAGCTCAACGAGCTC
GTCAACAGCCACGGGCGCTCCCAGGAGCAGCTCATCCGCCTGCAGGGTGAGCGCGACGAG
TGGAGGCGCGAGCGGGCCAAGGTGGAGACCAAGACGGTGAGCAAGGAGGTGGTGCGCCAC
GAGAAGGACCCGGTGCTGGAGAAAGAAGCAGAGCGGCTCCGCCAGGAGGTGCGGGAGGCG
GCCCAGAAGAGGCGGGCCGCGGAGGACGCGGTGTACGAGCTGCAGAGCAAGCGCCTGCTG
CTGGAGAGGAGGAAGCCCGAGGAGAAGGTGGTGGTGCAGGAGGTGGTGGTCACCCAGAAG
GACCCGAAGCTGCGCGAGGAGCACAGCCGGCTGAGCGGGAGCCTGGATGAGGAGGTGGGC
CGGCGGCGCCAGCTAGAGCTTGAGGTGCAGCAGCTGCGGGCCGGCGTGGAGGAGCAGGAG
GGCCTGCTCAGCTTCCAGGAGGACCGCAGCAAGAAGCTGGCCGTGGAGAGGGAGCTGCGG
CAGCTGACCTTGAGGATCCAGGAGCTCGAGAAGCGGCCTCCCACGGTGCAGGAGAAGATC
ATCATGGAGGAAGTGGTCAAGCTGGAGAAGGACCCGGACCTGGAGAAGTCCACGGAAGCC
CTGCGGTGGGACCTGGACCAGGAGAAGACCCAGGTAACCGAGCTGAATCGGGAGTGCAAG
AACCTGCAGGTCCAGATTGACGTCCTCCAGAAAGCCAAATCGCAGGAGAAGACCATCTAC
AAGGAAGTGATCCGGGTGCAGAAGGACCGCGTCCTGGAAGATGAGCGGGCCCGCGTGTGG
GAGATGCTCAACAGGGAGCGCACGGCCCGGCAGGCCCGGGAGGAGGAGGCACGGCGCCTG
CGGGAGCGCATTGACCGGGCCGAGACGCTGGGGAGAACCTGGTCCCGGGAGGAGTCCGAG
CTGCAGAGGGCCCGGGACCAGGCCGACCAGGAGTGTGGGCGGCTGCAGCAGGAGCTGCGG
GCTCTGGAGAGGCAGAAGCAGCAGCAGACACTGCAGCTGCAGGAGGAGTCGAAGCTGCTC
AGCCAGAAGACGGAGAGCGAGCGACAGAAGGCGGCCCAGCGGGGCCAGGAGCTCTCGCGG
CTGGAGGCGGCCATCCTCCGCGAGAAGGACCAGATCTACGAGAAGGAGCGGACGCTCCGG
GACCTCCACGCCAAGGTGAGCCGGGAGGAGCTCAGCCAGGAGACCCAGACGCGAGAGACC
AACCTTTCCACCAAGATCTCCATCCTGGAACCCGAGACGGGGAAGGACATGTCCCCATAC
GAGGCCTACAAGAGGGGCATCATCGACAGGGGCCAGTACTTGCAGCTGCAGGAGCTCGAG
TGTGACTGGGAGGAGGTCACCACCTCGGGGCCCTGTGGGGAGGAGTCTGTGCTCCTGGAC
CGCAAGAGCGGGAAGCAGTACTCCATCGAGGCCGCCCTCCGCTGCCGGCGCATCTCTAAG
GAGGAGTACCATCTGTACAAGGACGGCCACCTGCCCATCTCCGAGTTTGCGCTGCTTGTA
GCTGGGGAGACCAAGCCAAGCTCCTCACTCTCCATCGGCTCTATCATCTCCAAGTCCCCG
CTCGCCTCCCCGGCCCCCCAGAGCACCAGTTTCTTTTCTCCCAGCTTCTCTCTCGGGCTC
GGTGATGACAGCTTCCCTATCGCCGGGATCTATGACACAACCACAGACAACAAGTGCAGC
ATCAAGACGGCCGTGGCCAAGAACATGCTGGACCCCATCACTGGGCAGAAGCTACTGGAG
GCCCAGGCGGCCACAGGGGGCATCGTGGACCTGCTCAGCCGTGAGCGCTACTCTGTGCAC
AAGGCGATGGAGAGGGGCCTGATCGAGAACACCTCCACACAGAGGCTGCTTAACGCCCAG
AAGGCCTTCACCGGCATCGAGGACCCCGTCACCAAGAAGAGGCTCTCGGTGGGCGAGGCC
GTCCAGAAGGGCTGGATGCCCCGGGAGAGCGTGCTCCCACACCTGCAGGTGCAGCACCTG
ACCGGGGGGCTCATCGACCCCAAGAGGACAGGCCGCATCCCCATCCAGCAGGCCCTCCTC
TCCGGGATGATCAGTGAAGAGCTGGCCCAGCTCCTGCAGGACGAGTCCAGCTACGAGAAG
GATTTGACAGACCCCATCTCCAAGGAACGGCTGAGCTACAAGGAGGCCATGGGCCGCTGC
CGCAAAGACCCCCTGAGCGGCCTGCTGCTCCTGCCAGCGGCACTGGAGGGGTACCGCTGC
TACCGCTCCGCCTCCCCCACCGTCCCGCGCTCCCTTCGC

ORF Protein

Cloned ORF protein sequence for pFN21AB3462 Download

>KIBB3462  2033 aa
MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILET
QKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIK
QLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIA
EHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLS
ALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPA
VGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKY
SPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDS
ICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASR
LASELQALKQKLATVQSRLKASAVESLRPSQQAPSGSDLANPQAQKLLTQMTRLDGDLGQ
IERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVG
PAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAP
IPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQ
QRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGP
SQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSA
PKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDA
KQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVV
EFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVT
QVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVV
KVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVK
KVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIF
QVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRH
ERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRH
EKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQK
DPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELR
QLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECK
NLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRL
RERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLL
SQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRET
NLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLD
RKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSP
LASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLE
AQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEA
VQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEK
DLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3462 Download

>pFN21AB3462  10804 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCAAGGGGCTGAGCAAAGGCTCCCAGGGGAA
GGGGTCCCCCAAGGGCTCCCCCGCCAAGGGGTCCCCCAAAGGCTCCCCCAGCAGGCACAG
CCGGGCTGCCACCCAGGAGCTGGCCCTTCTCATCTCCCGCATGCAAGCCAACGCCGACCA
GGTGGAGCGGGACATCCTGGAGACGCAGAAGAGGCTGCAGCAGGACCGGCTGAACAGTGA
GCAGAGCCAGGCCCTGCAGCACCAGCAGGAGACGGGCCGCAGCCTGAAGGAGGCTGAGGT
GCTGCTCAAGGACCTCTTCCTGGACGTGGACAAGGCCCGGCGGCTCAAGCACCCGCAGGC
TGAGGAGATTGAGAAGGACATCAAGCAGCTGCACGAGCGGGTGACCCAGGAGTGTGCGGA
GTACCGTGCCCTGTACGAGAAGATGGTGCTGCCCCCCGACGTGGGACCCAGGGTCGACTG
GGCACGCGTGCTGGAGCAGAAACAGAAGCAGGTCTGCGCAGGCCAGTACGGGCCGGGCAT
GGCGGAGCTGGAGCAACAGATCGCCGAGCACAACATCCTGCAGAAGGAGATCGACGCCTA
TGGGCAGCAGCTGCGGAGCCTCGTGGGGCCGGATGCAGCCACCATCCGGAGCCAATACCG
AGACCTACTGAAGGCGGCGTCGTGGCGTGGGCAGAGCCTGGGCAGCCTGTACACGCACCT
CCAGGGCTGCACGCGGCAGCTGAGCGCCCTGGCTGAGCAGCAGCGCCGCATCCTGCAGCA
GGACTGGAGCGACCTCATGGCCGACCCTGCGGGCGTGCGGCGGGAGTACGAGCACTTCAA
GCAGCACGAGCTGCTGAGTCAGGAGCAGAGCGTGAACCAGCTGGAGGACGACGGCGAGCG
CATGGTGGAGCTGCGGCACCCCGCGGTGGGGCCCATCCAGGCCCACCAGGAGGCCCTGAA
GATGGAGTGGCAGAACTTCCTGAACCTGTGTATCTGCCAGGAGACCCAGCTGCAGCACGT
GGAGGACTACCGCCGGTTCCAGGAAGAGGCCGACTCAGTCAGCCAGACCCTGGCGAAGCT
CAACTCCAACTTGGATGCCAAGTACAGCCCTGCACCTGGGGGCCCCCCTGGCGCCCCCAC
AGAGCTGCTGCAACAGCTGGAGGCAGAGGAAAAACGGCTGGCCGTCACCGAGAGGGCCAC
TGGGGACCTGCAGCGGCGAAGCCGGGATGTGGCCCCTCTGCCACAGCGAAGAAACCCCCC
TCAGCAGCCCCTGCACGTGGACAGCATCTGCGACTGGGACTCAGGAGAAGTGCAGCTGCT
GCAGGGTGAGCGGTATAAGCTGGTAGATAACACTGACCCGCACGCCTGGGTCGTGCAGGG
CCCTGGCGGGGAGACCAAGCGTGCTCCCGCCGCCTGCTTCTGCATCCCAGCACCAGACCC
TGATGCTGTGGCCAGGGCCTCCCGGCTGGCCTCAGAGCTGCAGGCCCTGAAGCAGAAATT
GGCCACAGTCCAGAGCCGCCTGAAGGCCAGTGCTGTGGAGTCTCTTCGGCCCAGCCAGCA
GGCTCCATCTGGCTCAGACCTGGCCAACCCACAGGCCCAGAAGCTCCTGACACAGATGAC
CCGGCTGGATGGAGACCTGGGACAGATAGAGAGGCAGGTGCTGGCCTGGGCGCGGGCCCC
GCTGAGCCGCCCCACACCCTTGGAGGACTTGGAGGGCCGCATCCACAGCCATGAGGGCAC
AGCCCAGCGCCTGCAGAGCCTGGGAACGGAGAAGGAGACAGCCCAGAAGGAGTGCGAGGC
GTTTCTGTCCACGCGGCCCGTGGGCCCCGCTGCCCTGCAGCTGCCCGTAGCCCTCAACAG
CGTGAAGAACAAGTTCAGTGACGTGCAGGTTCTGTGCAGCCTCTACGGGGAGAAAGCCAA
GGCTGCCCTGGATCTGGAGCGGCAGATCCAGGATGCGGACAGGGTCATCCGAGGCTTCGA
GGCCACCCTGGTGCAGGAGGCCCCCATCCCTGCTGAACCGGGGGCTCTGCAGGAGAGGGT
CAGCGAGCTGCAGCGCCAGCGGAGGGAGCTGCTGGAACAGCAGACCTGCGTGCTGCGGCT
ACACCGCGCGCTGAAGGCCTCGGAGCACGCATGCGCTGCCCTGCAGAACAACTTCCAGGA
GTTCTGCCAAGACCTGCCTCGCCAGCAGCGCCAGGTGCGAGCCCTCACCGACCGCTACCA
CGCCGTAGGGGACCAGCTGGACCTGCGGGAGAAGGTGGTGCAGGATGCCGCCCTCACCTA
CCAGCAGTTCAAGAACTGCAAGGATAACCTGAGCTCCTGGCTGGAGCACCTGCCCCGCAG
CCAGGTGCGGCCCAGCGACGGCCCCAGCCAGATCGCCTACAAGCTGCAGGCGCAGAAGAG
GCTGACGCAGGAGATCCAGAGCCGAGAGCGGGACAGGGCCACAGCATCCCACCTCTCCCA
GGCCCTGCAGGCAGCGCTCCAGGACTATGAGCTCCAGGCAGACACCTACCGCTGCTCTTT
GGAGCCCACCCTGGCAGTGTCAGCCCCCAAGAGACCCCGAGTGGCTCCCCTGCAAGAGAG
CATCCAAGCCCAGGAGAAGAACCTTGCAAAGGCCTATACTGAGGTTGCAGCAGCACAGCA
GCAGCTGCTCCAGCAGCTGGAGTTTGCTAGAAAAATGCTGGAGAAGAAGGAGCTCAGTGA
GGACATCCGAAGGACCCATGATGCAAAGCAGGGCTCCGAGAGCCCTGCCCAAGCAGGGAG
AGAGTCAGAGGCCCTGAAGGCCCAGCTGGAAGAGGAGAGGAAGCGGGTGGCCCGGGTGCA
GCATGAGCTGGAGGCGCAGAGGAGCCAACTGCTGCAGCTGAGGACCCAGCGGCCCTTGGA
GAGGCTGGAGGAGAAGGAAGTGGTAGAGTTCTACCGGGACCCCCAGCTGGAGGGCAGCCT
GTCCAGGGTGAAGGCCCAGGTGGAGGAGGAGGGCAAGCGGCGGGCTGGCCTGCAGGCAGA
CCTGGAAGTGGCAGCCCAGAAGGTCGTGCAGCTGGAAAGCAAGAGGAAGACCATGCAGCC
TCATCTGCTGACCAAGGAGGTCACCCAGGTGGAGAGGGACCCCGGCCTGGACAGCCAGGC
GGCCCAGCTCAGGATCCAGATCCAGCAGCTCCGCGGGGAGGATGCCGTCATCTCGGCCCG
GCTGGAAGGGCTGAAGAAGGAGCTACTGGCCCTTGAGAAGAGGGAGGTGGACGTGAAGGA
GAAGGTCGTGGTGAAAGAGGTAGTCAAGGTGGAGAAGAATCTGGAAATGGTCAAGGCAGC
CCAGGCTCTGAGGCTGCAGATGGAGGAGGATGCTGCGCGGAGGAAGCAGGCGGAGGAGGC
TGTGGCCAAGCTACAGGCTCGCATCGAAGACCTGGAGCGGGCTATCAGCTCGGTGGAGCC
CAAGGTCATCGTGAAGGAGGTGAAGAAGGTGGAGCAGGACCCAGGGCTCCTCCAGGAGTC
CTCCAGGCTGAGGAGCCTCCTCGAGGAGGAGAGGACCAAGAACGCGACGCTGGCCAGGGA
GCTGAGCGACCTGCACAGCAAGTACAGCGTGGTGGAGAAGCAGAGGCCCAAAGTGCAGCT
CCAGGAGCGCGTCCACGAGATCTTCCAGGTGGATCCGGAGACAGAGCAGGAGATCACTCG
GCTCAAGGCCAAGCTGCAGGAGATGGCGGGCAAGAGGAGCGGTGTGGAGAAGGAGGTGGA
GAAGCTGCTGCCCGACCTGGAGGTCCTGCGGGCCCAGAAGCCCACGGTGGAGTACAAGGA
GGTGACCCAGGAGGTGGTGAGGCATGAGAGGAGCCCCGAGGTGCTGCGTGAGATCGACCG
CCTGAAGGCTCAGCTCAACGAGCTCGTCAACAGCCACGGGCGCTCCCAGGAGCAGCTCAT
CCGCCTGCAGGGTGAGCGCGACGAGTGGAGGCGCGAGCGGGCCAAGGTGGAGACCAAGAC
GGTGAGCAAGGAGGTGGTGCGCCACGAGAAGGACCCGGTGCTGGAGAAAGAAGCAGAGCG
GCTCCGCCAGGAGGTGCGGGAGGCGGCCCAGAAGAGGCGGGCCGCGGAGGACGCGGTGTA
CGAGCTGCAGAGCAAGCGCCTGCTGCTGGAGAGGAGGAAGCCCGAGGAGAAGGTGGTGGT
GCAGGAGGTGGTGGTCACCCAGAAGGACCCGAAGCTGCGCGAGGAGCACAGCCGGCTGAG
CGGGAGCCTGGATGAGGAGGTGGGCCGGCGGCGCCAGCTAGAGCTTGAGGTGCAGCAGCT
GCGGGCCGGCGTGGAGGAGCAGGAGGGCCTGCTCAGCTTCCAGGAGGACCGCAGCAAGAA
GCTGGCCGTGGAGAGGGAGCTGCGGCAGCTGACCTTGAGGATCCAGGAGCTCGAGAAGCG
GCCTCCCACGGTGCAGGAGAAGATCATCATGGAGGAAGTGGTCAAGCTGGAGAAGGACCC
GGACCTGGAGAAGTCCACGGAAGCCCTGCGGTGGGACCTGGACCAGGAGAAGACCCAGGT
AACCGAGCTGAATCGGGAGTGCAAGAACCTGCAGGTCCAGATTGACGTCCTCCAGAAAGC
CAAATCGCAGGAGAAGACCATCTACAAGGAAGTGATCCGGGTGCAGAAGGACCGCGTCCT
GGAAGATGAGCGGGCCCGCGTGTGGGAGATGCTCAACAGGGAGCGCACGGCCCGGCAGGC
CCGGGAGGAGGAGGCACGGCGCCTGCGGGAGCGCATTGACCGGGCCGAGACGCTGGGGAG
AACCTGGTCCCGGGAGGAGTCCGAGCTGCAGAGGGCCCGGGACCAGGCCGACCAGGAGTG
TGGGCGGCTGCAGCAGGAGCTGCGGGCTCTGGAGAGGCAGAAGCAGCAGCAGACACTGCA
GCTGCAGGAGGAGTCGAAGCTGCTCAGCCAGAAGACGGAGAGCGAGCGACAGAAGGCGGC
CCAGCGGGGCCAGGAGCTCTCGCGGCTGGAGGCGGCCATCCTCCGCGAGAAGGACCAGAT
CTACGAGAAGGAGCGGACGCTCCGGGACCTCCACGCCAAGGTGAGCCGGGAGGAGCTCAG
CCAGGAGACCCAGACGCGAGAGACCAACCTTTCCACCAAGATCTCCATCCTGGAACCCGA
GACGGGGAAGGACATGTCCCCATACGAGGCCTACAAGAGGGGCATCATCGACAGGGGCCA
GTACTTGCAGCTGCAGGAGCTCGAGTGTGACTGGGAGGAGGTCACCACCTCGGGGCCCTG
TGGGGAGGAGTCTGTGCTCCTGGACCGCAAGAGCGGGAAGCAGTACTCCATCGAGGCCGC
CCTCCGCTGCCGGCGCATCTCTAAGGAGGAGTACCATCTGTACAAGGACGGCCACCTGCC
CATCTCCGAGTTTGCGCTGCTTGTAGCTGGGGAGACCAAGCCAAGCTCCTCACTCTCCAT
CGGCTCTATCATCTCCAAGTCCCCGCTCGCCTCCCCGGCCCCCCAGAGCACCAGTTTCTT
TTCTCCCAGCTTCTCTCTCGGGCTCGGTGATGACAGCTTCCCTATCGCCGGGATCTATGA
CACAACCACAGACAACAAGTGCAGCATCAAGACGGCCGTGGCCAAGAACATGCTGGACCC
CATCACTGGGCAGAAGCTACTGGAGGCCCAGGCGGCCACAGGGGGCATCGTGGACCTGCT
CAGCCGTGAGCGCTACTCTGTGCACAAGGCGATGGAGAGGGGCCTGATCGAGAACACCTC
CACACAGAGGCTGCTTAACGCCCAGAAGGCCTTCACCGGCATCGAGGACCCCGTCACCAA
GAAGAGGCTCTCGGTGGGCGAGGCCGTCCAGAAGGGCTGGATGCCCCGGGAGAGCGTGCT
CCCACACCTGCAGGTGCAGCACCTGACCGGGGGGCTCATCGACCCCAAGAGGACAGGCCG
CATCCCCATCCAGCAGGCCCTCCTCTCCGGGATGATCAGTGAAGAGCTGGCCCAGCTCCT
GCAGGACGAGTCCAGCTACGAGAAGGATTTGACAGACCCCATCTCCAAGGAACGGCTGAG
CTACAAGGAGGCCATGGGCCGCTGCCGCAAAGACCCCCTGAGCGGCCTGCTGCTCCTGCC
AGCGGCACTGGAGGGGTACCGCTGCTACCGCTCCGCCTCCCCCACCGTCCCGCGCTCCCT
TCGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT