Restriction map for
pFN21AB3462
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# NO CUT ENZYMES ( 57 enzymes) #
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AclI AcsI AflII AgeI AocI
ApoI AscI AseI AsnI AvrII
BciVI BfrI BlnI BsiWI BsmI
BspDI BspHI BsrDI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eco81I EcoRI EcoRV EcoT22I
FseI HindIII HpaI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AatII 4519 GACGTC GACGT^C
Acc65I 6050 GGTACC G^GTACC
AccI 448 GTCGAC GT^[AC][GT]AC
AhdI 440 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5635 GTGCAC G^TGCAC
ApaLI 5635 GTGCAC G^TGCAC
Asp718I 6050 GGTACC G^GTACC
AspEI 440 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 490 TGCGCA TGC^GCA
BcgI 2712 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5888 TGATCA T^GATCA
BspLU11I 5087 ACATGT A^CATGT
BssHII 2081 GCGCGC G^CGCGC
DrdI 3709 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5528 CGGCCG C^GGCCG
Eam1105I 440 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5528 CGGCCG C^GGCCG
Eco52I 5528 CGGCCG C^GGCCG
FbaI 5888 TGATCA T^GATCA
FspI 490 TGCGCA TGC^GCA
KpnI 6050 GGTACC GGTAC^C
MluI 459 ACGCGT A^CGCGT
MseI 5690 TTAA T^TAA
NcoI 5987 CCATGG C^CATGG
SalI 448 GTCGAC G^TCGAC
SapI 5896 GAAGAGC GCTCTTC(1/4)
Tru9I 5690 TTAA T^TAA
Tsp509I 1472 AATT ^AATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 620 TCCGGA T^CCGGA
3609 TCCGGA
AflIII 459 ACGCGT A^C[GA][CT]GT
5087 ACATGT
Aor51HI 2448 AGCGCT AGC^GCT
5624 AGCGCT
BbrPI 991 CACGTG CAC^GTG
1249 CACGTG
BfaI 2642 CTAG C^TAG
4213 CTAG
BsaAI 991 CACGTG [CT]AC^GT[GA]
1249 CACGTG
BsaBI 3108 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5055 GATCTCCATC
BsaWI 620 TCCGGA [AT]^CCGG[AT]
3609 TCCGGA
BseAI 620 TCCGGA T^CCGGA
3609 TCCGGA
Bsp1407I 683 TGTACA T^GTACA
5294 TGTACA
BspEI 620 TCCGGA T^CCGGA
3609 TCCGGA
BsrGI 683 TGTACA T^GTACA
5294 TGTACA
Eco47III 2448 AGCGCT AGC^GCT
5624 AGCGCT
HincII 448 GTCGAC GT[CT]^[GA]AC
3841 GTCAAC
HindII 448 GTCGAC GT[CT]^[GA]AC
3841 GTCAAC
MaeI 2642 CTAG C^TAG
4213 CTAG
MamI 3108 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5055 GATCTCCATC
MroI 620 TCCGGA T^CCGGA
3609 TCCGGA
PmaCI 991 CACGTG CAC^GTG
1249 CACGTG
PmlI 991 CACGTG CAC^GTG
1249 CACGTG
ScaI 5135 AGTACT AGT^ACT
5237 AGTACT
SexAI 152 ACCAGGT A^CC[AT]GGT
4717 ACCTGGT
SphI 134 GCATGC GCATG^C
2104 GCATGC
SspBI 683 TGTACA T^GTACA
5294 TGTACA
TfiI 3252 GAATC G^A[AT]TC
4485 GAATC
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# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI Sse8387I StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI