Restriction map for
pFN21AB3462
(Linear) (Six-base) MAPSORT of: KIBB3462.seq Check: 7987 from: 1 to: 6099
KIBB3462.orf
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1620 2312 5318 5796 5810 6099
Size: 1620 692 3006 478 14 289
Fragments arranged by size:
3006 1620 692 478 289 14
AatII G_ACGT'C
Cuts at: 0 4523 6099
Size: 4523 1576
AccI GT'mk_AC
Cuts at: 0 449 6099
Size: 449 5650
Acc65I G'GTAC_C
Cuts at: 0 6050 6099
Size: 6050 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 664 952 1483 1516 2107 2770 3187
Size: 280 384 288 531 33 591 663 417
Cuts at: 3187 3838 6099
Size: 651 2261
Fragments arranged by size:
2261 663 651 591 531 417 384 288
280 33
AfeI AGC'GCT
Cuts at: 0 2450 5626 6099
Size: 2450 3176 473
Fragments arranged by size:
3176 2450 473
AflIII A'CryG_T
Cuts at: 0 459 5087 6099
Size: 459 4628 1012
Fragments arranged by size:
4628 1012 459
AhdI GACnn_n'nnGTC
Cuts at: 0 445 6099
Size: 445 5654
AleI CACnn'nnGTG
Cuts at: 0 1963 3061 6099
Size: 1963 1098 3038
Fragments arranged by size:
3038 1963 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2335 4090 4519 6099
Size: 2335 1755 429 1580
Fragments arranged by size:
2335 1755 1580 429
AlwNI CAG_nnn'CTG
Cuts at: 0 114 228 585 672 1044 1245 1262 1293
Size: 114 114 357 87 372 201 17 31
Cuts at: 1293 1414 1461 1635 1725 1734 1866 2439 2619
Size: 121 47 174 90 9 132 573 180
Cuts at: 2619 2631 3282 4233 4794 4830 5685 5817 5913
Size: 12 651 951 561 36 855 132 96
Cuts at: 5913 6099
Size: 186
Fragments arranged by size:
951 855 651 573 561 372 357 201
186 180 174 132 132 121 114 114
96 90 87 47 36 31 17 12
9
ApaI G_GGCC'C
Cuts at: 0 908 1099 1357 1653 1801 3729 4611 4752
Size: 908 191 258 296 148 1928 882 141
Cuts at: 4752 5192 6099
Size: 440 907
Fragments arranged by size:
1928 908 907 882 440 296 258 191
148 141
ApaLI G'TGCA_C
Cuts at: 0 5635 6099
Size: 5635 464
AvaI C'yCGr_G
Cuts at: 0 2531 2786 3475 3790 4096 4345 4655 4724
Size: 2531 255 689 315 306 249 310 69
Cuts at: 4724 4751 5071 5155 5184 5452 5780 6099
Size: 27 320 84 29 268 328 319
Fragments arranged by size:
2531 689 328 320 319 315 310 306
268 255 249 84 69 29 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 948 981 6099
Size: 948 33 5118
Fragments arranged by size:
5118 948 33
BamHI G'GATC_C
Cuts at: 0 3107 3606 4334 6099
Size: 3107 499 728 1765
Fragments arranged by size:
3107 1765 728 499
BanI G'GyrC_C
Cuts at: 0 890 1105 4205 4673 6050 6099
Size: 890 215 3100 468 1377 49
Fragments arranged by size:
3100 1377 890 468 215 49
BanII G_rGCy'C
Cuts at: 0 52 908 1099 1357 1653 1801 2001 2178
Size: 52 856 191 258 296 148 200 177
Cuts at: 2178 2290 2468 2501 2669 2710 2719 3395 3443
Size: 112 178 33 168 41 9 676 48
Cuts at: 3443 3729 3790 3839 4346 4611 4752 4803 4913
Size: 286 61 49 507 265 141 51 110
Cuts at: 4913 5012 5156 5192 5459 5831 6099
Size: 99 144 36 267 372 268
Fragments arranged by size:
856 676 507 372 296 286 268 267
265 258 200 191 178 177 168 148
144 141 112 110 99 61 52 51
49 48 41 36 33 9
BbeI G_GCGC'C
Cuts at: 0 1109 4209 4677 6099
Size: 1109 3100 468 1422
Fragments arranged by size:
3100 1422 1109 468
BbsI GAAGACnn'nnnn_
Cuts at: 0 3028 3368 4471 4555 4873 6099
Size: 3028 340 1103 84 318 1226
Fragments arranged by size:
3028 1226 1103 340 318 84
BbvCI CC'TCA_GC
Cuts at: 0 269 335 1235 2833 3463 6099
Size: 269 66 900 1598 630 2636
Fragments arranged by size:
2636 1598 900 630 269 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2701 2735 6099
Size: 2701 34 3364
Fragments arranged by size:
3364 2701 34
BclI T'GATC_A
Cuts at: 0 5888 6099
Size: 5888 211
BglI GCCn_nnn'nGGC
Cuts at: 0 1103 1709 2443 2752 4651 5410 5587 5701
Size: 1103 606 734 309 1899 759 177 114
Cuts at: 5701 6099
Size: 398
Fragments arranged by size:
1899 1103 759 734 606 398 309 177
114
BglII A'GATC_T
Cuts at: 0 3593 4952 5054 6099
Size: 3593 1359 102 1045
Fragments arranged by size:
3593 1359 1045 102
BlpI GC'TnA_GC
Cuts at: 0 13 715 728 1657 3517 3824 4171 4267
Size: 13 702 13 929 1860 307 347 96
Cuts at: 4267 4858 5011 5614 5971 6099
Size: 591 153 603 357 128
Fragments arranged by size:
1860 929 702 603 591 357 347 307
153 128 96 13 13
Bme1580I G_kGCm'C
Cuts at: 0 405 459 908 1099 1357 1653 1714 1801
Size: 405 54 449 191 258 296 61 87
Cuts at: 1801 3729 4611 4752 5192 5639 6099
Size: 1928 882 141 440 447 460
Fragments arranged by size:
1928 882 460 449 447 440 405 296
258 191 141 87 61 54
BmgBI CAC'GTC
Cuts at: 0 300 435 1857 3207 6099
Size: 300 135 1422 1350 2892
Fragments arranged by size:
2892 1422 1350 300 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 462 1184 1278 5175 5570 6099
Size: 462 722 94 3897 395 529
Fragments arranged by size:
3897 722 529 462 395 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1044 5646 5688 6099
Size: 1044 4602 42 411
Fragments arranged by size:
4602 1044 411 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 487 544 679 780 877 1153 1930
Size: 193 294 57 135 101 97 276 777
Cuts at: 1930 2317 2437 2452 2608 2653 2674 2824 2881
Size: 387 120 15 156 45 21 150 57
Cuts at: 2881 2923 3388 3430 3441 3559 3733 3997 4102
Size: 42 465 42 11 118 174 264 105
Cuts at: 4102 4423 4441 4510 4825 4942 5596 6064 6099
Size: 321 18 69 315 117 654 468 35
Fragments arranged by size:
777 654 468 465 387 321 315 294
276 264 193 174 156 150 135 120
118 117 105 101 97 69 57 57
45 42 42 35 21 18 15 11
Bpu10I CC'TnA_GC
Cuts at: 0 269 335 1235 2284 2833 3103 3463 6013
Size: 269 66 900 1049 549 270 360 2550
Cuts at: 6013 6099
Size: 86
Fragments arranged by size:
2550 1049 900 549 360 270 269 86
66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 304 3208 3622 4240 4350 6099
Size: 265 39 2904 414 618 110 1749
Fragments arranged by size:
2904 1749 618 414 265 110 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 629 970 1360 1601 2520 3919 5014 5029
Size: 629 341 390 241 919 1399 1095 15
Cuts at: 5029 5341 6099
Size: 312 758
Fragments arranged by size:
1399 1095 919 758 629 390 341 312
241 15
BsaAI yAC'GTr
Cuts at: 0 993 1251 6099
Size: 993 258 4848
Fragments arranged by size:
4848 993 258
BsaBI GATnn'nnATC
Cuts at: 0 3112 5059 6099
Size: 3112 1947 1040
Fragments arranged by size:
3112 1947 1040
BsaHI Gr'CG_yC
Cuts at: 0 569 652 1106 4206 4520 4674 6099
Size: 569 83 454 3100 314 154 1425
Fragments arranged by size:
3100 1425 569 454 314 154 83
BsaWI w'CCGG_w
Cuts at: 0 620 3609 6099
Size: 620 2989 2490
Fragments arranged by size:
2989 2490 620
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2272 2302 3841 3871 5143 5173 5941 5971
Size: 2272 30 1539 30 1272 30 768 30
Cuts at: 5971 6099
Size: 128
Fragments arranged by size:
2272 1539 1272 768 128 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 2782 2829 2878 2953 2956 3313 3343
Size: 352 2430 47 49 75 3 357 30
Cuts at: 3343 3433 3463 3480 3493 3496 3684 3798 4101
Size: 90 30 17 13 3 188 114 303
Cuts at: 4101 4114 4171 4204 4264 4675 4678 4744 4858
Size: 13 57 33 60 411 3 66 114
Cuts at: 4858 5020 5185 5215 5296 5352 5866 6099
Size: 162 165 30 81 56 514 233
Fragments arranged by size:
2430 514 411 357 352 303 233 188
165 162 114 114 90 81 75 66
60 57 56 49 47 33 30 30
30 17 13 13 3 3 3
BseYI C'CCAG_C
Cuts at: 0 82 980 1401 1525 1718 2326 2338 2756
Size: 82 898 421 124 193 608 12 418
Cuts at: 2756 2840 2897 3098 4707 4895 5341 5440 5906
Size: 84 57 201 1609 188 446 99 466
Cuts at: 5906 6099
Size: 193
Fragments arranged by size:
1609 898 608 466 446 421 418 201
193 193 188 124 99 84 82 57
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 687 1070 1231 1306 1369 1879 1890
Size: 349 338 383 161 75 63 510 11
Cuts at: 1890 1987 2254 2812 3022 3511 3589 4135 4246
Size: 97 267 558 210 489 78 546 111
Cuts at: 4246 4387 4597 5535 5830 6099
Size: 141 210 938 295 269
Fragments arranged by size:
938 558 546 510 489 383 349 338
295 269 267 210 210 161 141 111
97 78 75 63 11
BsiEI CG_ry'CG
Cuts at: 0 2187 3813 5531 6099
Size: 2187 1626 1718 568
Fragments arranged by size:
2187 1718 1626 568
BsiHKAI G_wGCw'C
Cuts at: 0 545 809 1380 2102 2290 2303 2468 2669
Size: 545 264 571 722 188 13 165 201
Cuts at: 2669 3839 4163 4346 4913 5012 5156 5213 5425
Size: 1170 324 183 567 99 144 57 212
Cuts at: 5425 5639 5795 6099
Size: 214 156 304
Fragments arranged by size:
1170 722 571 567 545 324 304 264
214 212 201 188 183 165 156 144
99 57 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 238 4696 5068 6099
Size: 169 69 4458 372 1031
Fragments arranged by size:
4458 1031 372 169 69
Bsp1286I G_dGCh'C
Cuts at: 0 52 405 459 545 809 908 1099 1357
Size: 52 353 54 86 264 99 191 258
Cuts at: 1357 1380 1653 1714 1801 2001 2102 2178 2290
Size: 23 273 61 87 200 101 76 112
Cuts at: 2290 2303 2468 2501 2669 2710 2719 3395 3443
Size: 13 165 33 168 41 9 676 48
Cuts at: 3443 3729 3790 3839 4163 4346 4611 4752 4803
Size: 286 61 49 324 183 265 141 51
Cuts at: 4803 4913 5012 5156 5192 5213 5425 5459 5639
Size: 110 99 144 36 21 212 34 180
Cuts at: 5639 5795 5831 6099
Size: 156 36 268
Fragments arranged by size:
676 353 324 286 273 268 265 264
258 212 200 191 183 180 168 165
156 144 141 112 110 101 99 99
87 86 76 61 61 54 52 51
49 48 41 36 36 34 33 23
21 13 9
BspEI T'CCGG_A
Cuts at: 0 620 3609 6099
Size: 620 2989 2490
Fragments arranged by size:
2989 2490 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 1190 1620 1851 2068 2156 2225 2312
Size: 474 716 430 231 217 88 69 87
Cuts at: 2312 3533 4497 4511 5318 5618 5796 5810 6099
Size: 1221 964 14 807 300 178 14 289
Fragments arranged by size:
1221 964 807 716 474 430 300 289
231 217 178 88 87 69 14 14
BsrBI CCG'CTC
Cuts at: 0 162 372 1305 1914 2403 3372 3674 3912
Size: 162 210 933 609 489 969 302 238
Cuts at: 3912 3993 4175 4605 4968 5228 5400 6017 6065
Size: 81 182 430 363 260 172 617 48
Cuts at: 6065 6099
Size: 34
Fragments arranged by size:
969 933 617 609 489 430 363 302
260 238 210 182 172 162 81 48
34
BsrFI r'CCGG_y
Cuts at: 0 200 1007 4166 4199 4240 5264 5710 6099
Size: 200 807 3159 33 41 1024 446 389
Fragments arranged by size:
3159 1024 807 446 389 200 41 33
BsrGI T'GTAC_A
Cuts at: 0 683 5294 6099
Size: 683 4611 805
Fragments arranged by size:
4611 805 683
BssHII G'CGCG_C
Cuts at: 0 2081 6099
Size: 2081 4018
BssSI C'ACGA_G
Cuts at: 0 367 595 820 3589 3958 6099
Size: 367 228 225 2769 369 2141
Fragments arranged by size:
2769 2141 369 367 228 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 825 1245 1293 2110 2619 4233 4794
Size: 585 240 420 48 817 509 1614 561
Cuts at: 4794 4830 6099
Size: 36 1269
Fragments arranged by size:
1614 1269 817 585 561 509 420 240
48 36
BstEII G'GTnAC_C
Cuts at: 0 375 3054 3756 4128 4473 5175 6099
Size: 375 2679 702 372 345 702 924
Fragments arranged by size:
2679 924 702 702 375 372 345
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1509 1674 4774 6099
Size: 1509 165 3100 1325
Fragments arranged by size:
3100 1509 1325 165
BstYI r'GATC_y
Cuts at: 0 1905 1919 2387 3107 3113 3593 3606 4334
Size: 1905 14 468 720 6 480 13 728
Cuts at: 4334 4952 5054 5486 6099
Size: 618 102 432 613
Fragments arranged by size:
1905 728 720 618 613 480 468 432
102 14 13 6
BtgI C'CryG_G
Cuts at: 0 896 1793 3126 3737 3849 4037 4301 4361
Size: 896 897 1333 611 112 188 264 60
Cuts at: 4361 4430 5531 5987 6099
Size: 69 1101 456 112
Fragments arranged by size:
1333 1101 897 896 611 456 264 188
112 112 69 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5659 6099
Size: 5659 440
BtsI GCAGTG_nn'
Cuts at: 0 2516 4828 6099
Size: 2516 2312 1271
Fragments arranged by size:
2516 2312 1271
DrdI GACnn_nn'nnGTC
Cuts at: 0 3715 6099
Size: 3715 2384
EaeI y'GGCC_r
Cuts at: 0 773 1154 1424 1475 1556 3338 3506 4298
Size: 773 381 270 51 81 1782 168 792
Cuts at: 4298 4928 5304 5528 5534 5588 6099
Size: 630 376 224 6 54 511
Fragments arranged by size:
1782 792 773 630 511 381 376 270
224 168 81 54 51 6
EagI C'GGCC_G
Cuts at: 0 5528 6099
Size: 5528 571
EarI CTCTTCn'nnn_
Cuts at: 0 178 295 1013 1523 2365 2759 3187 4021
Size: 178 117 718 510 842 394 428 834
Cuts at: 4021 5731 5891 6099
Size: 1710 160 208
Fragments arranged by size:
1710 842 834 718 510 428 394 208
178 160 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 3340 3865 3988 6057 6099
Size: 532 2808 525 123 2069 42
Fragments arranged by size:
2808 2069 532 525 123 42
EcoICRI GAG'CTC
Cuts at: 0 2288 2466 2667 3837 4344 4911 5010 5154
Size: 2288 178 201 1170 507 567 99 144
Cuts at: 5154 6099
Size: 945
Fragments arranged by size:
2288 1170 945 567 507 201 178 144
99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 280 487 544 664 679 780 877
Size: 193 87 207 57 120 15 101 97
Cuts at: 877 952 1153 1483 1516 1930 2107 2317 2437
Size: 75 201 330 33 414 177 210 120
Cuts at: 2437 2452 2608 2653 2674 2770 2824 2881 2923
Size: 15 156 45 21 96 54 57 42
Cuts at: 2923 3187 3388 3430 3441 3559 3733 3838 3997
Size: 264 201 42 11 118 174 105 159
Cuts at: 3997 4102 4423 4441 4510 4825 4942 5596 6064
Size: 105 321 18 69 315 117 654 468
Cuts at: 6064 6099
Size: 35
Fragments arranged by size:
654 468 414 330 321 315 264 210
207 201 201 193 177 174 159 156
120 120 118 117 105 105 101 97
96 87 75 69 57 57 54 45
42 42 35 33 21 18 15 15
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 556 643 751 1966 2431 2581 2853
Size: 262 294 87 108 1215 465 150 272
Cuts at: 2853 3475 3478 4186 4456 5109 6099
Size: 622 3 708 270 653 990
Fragments arranged by size:
1215 990 708 653 622 465 294 272
270 262 150 108 87 3
EcoO109I rG'GnC_Cy
Cuts at: 0 38 65 225 285 439 904 927 1095
Size: 38 27 160 60 154 465 23 168
Cuts at: 1095 1096 1179 1353 1354 1430 1458 1650 1798
Size: 1 83 174 1 76 28 192 148
Cuts at: 1798 1974 2436 2687 2745 2837 2892 3072 3432
Size: 176 462 251 58 92 55 180 360
Cuts at: 3432 3717 3966 4140 4260 4410 4449 4748 4980
Size: 285 249 174 120 150 39 299 232
Cuts at: 4980 5188 5189 5655 5721 5871 6099
Size: 208 1 466 66 150 228
Fragments arranged by size:
466 465 462 360 299 285 251 249
232 228 208 192 180 176 174 174
168 160 154 150 150 148 120 92
83 76 66 60 58 55 39 38
28 27 23 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 6099
Size: 306 5793
FspI TGC'GCA
Cuts at: 0 492 6099
Size: 492 5607
HaeII r_GCGC'y
Cuts at: 0 722 741 1109 1725 2031 2166 2452 3858
Size: 722 19 368 616 306 135 286 1406
Cuts at: 3858 4074 4209 4677 5628 6099
Size: 216 135 468 951 471
Fragments arranged by size:
1406 951 722 616 471 468 368 306
286 216 135 135 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 1258 1290 5941 5973 6099
Size: 431 32 795 32 4651 32 126
Fragments arranged by size:
4651 795 431 126 32 32 32
HincII GTy'rAC
Cuts at: 0 450 3843 6099
Size: 450 3393 2256
Fragments arranged by size:
3393 2256 450
KasI G'GCGC_C
Cuts at: 0 1105 4205 4673 6099
Size: 1105 3100 468 1426
Fragments arranged by size:
3100 1426 1105 468
KpnI G_GTAC'C
Cuts at: 0 6054 6099
Size: 6054 45
MluI A'CGCG_T
Cuts at: 0 459 6099
Size: 459 5640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 6099
Size: 1085 5014
MscI TGG'CCA
Cuts at: 0 1426 1477 1558 3340 3508 5536 6099
Size: 1426 51 81 1782 168 2028 563
Fragments arranged by size:
2028 1782 1426 563 168 81 51
MslI CAynn'nnrTG
Cuts at: 0 1703 1963 3061 6099
Size: 1703 260 1098 3038
Fragments arranged by size:
3038 1703 1098 260
MspA1I CmG'CkG
Cuts at: 0 363 585 714 855 898 984 1131 1188
Size: 363 222 129 141 43 86 147 57
Cuts at: 1188 1290 1656 1804 1815 2034 2211 2486 2619
Size: 102 366 148 11 219 177 275 133
Cuts at: 2619 2631 2760 2832 2844 2901 3006 3128 4039
Size: 12 129 72 12 57 105 122 911
Cuts at: 4039 4233 4323 4836 4899 5145 5261 5996 6037
Size: 194 90 513 63 246 116 735 41
Cuts at: 6037 6056 6099
Size: 19 43
Fragments arranged by size:
911 735 513 366 363 275 246 222
219 194 177 148 147 141 133 129
129 122 116 105 102 90 86 72
63 57 57 43 43 41 19 12
12 11
NaeI GCC'GGC
Cuts at: 0 4168 4201 4242 5266 6099
Size: 4168 33 41 1024 833
Fragments arranged by size:
4168 1024 833 41 33
NarI GG'CG_CC
Cuts at: 0 1106 4206 4674 6099
Size: 1106 3100 468 1425
Fragments arranged by size:
3100 1425 1106 468
NcoI C'CATG_G
Cuts at: 0 5987 6099
Size: 5987 112
NgoMIV G'CCGG_C
Cuts at: 0 4166 4199 4240 5264 6099
Size: 4166 33 41 1024 835
Fragments arranged by size:
4166 1024 835 41 33
NspI r_CATG'y
Cuts at: 0 138 2108 5091 5547 6099
Size: 138 1970 2983 456 552
Fragments arranged by size:
2983 1970 552 456 138
PasI CC'CwG_GG
Cuts at: 0 27 443 3436 6099
Size: 27 416 2993 2663
Fragments arranged by size:
2993 2663 416 27
PciI A'CATG_T
Cuts at: 0 5087 6099
Size: 5087 1012
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 1044 2631 3768 6099
Size: 114 930 1587 1137 2331
Fragments arranged by size:
2331 1587 1137 930 114
PfoI T'CCnGG_A
Cuts at: 0 170 293 1013 1922 2129 2453 3446 3575
Size: 170 123 720 909 207 324 993 129
Cuts at: 3575 4274 4337 4592 4976 5063 5213 5881 6099
Size: 699 63 255 384 87 150 668 218
Fragments arranged by size:
993 909 720 699 668 384 324 255
218 207 170 150 129 123 87 63
PmlI CAC'GTG
Cuts at: 0 993 1251 6099
Size: 993 258 4848
Fragments arranged by size:
4848 993 258
PpuMI rG'GwC_Cy
Cuts at: 0 38 65 285 439 1179 2687 2837 2892
Size: 38 27 220 154 740 1508 150 55
Cuts at: 2892 3072 3432 3717 3966 4140 4410 4449 4980
Size: 180 360 285 249 174 270 39 531
Cuts at: 4980 5721 6099
Size: 741 378
Fragments arranged by size:
1508 741 740 531 378 360 285 270
249 220 180 174 154 150 55 39
38 27
PspOMI G'GGCC_C
Cuts at: 0 904 1095 1353 1649 1797 3725 4607 4748
Size: 904 191 258 296 148 1928 882 141
Cuts at: 4748 5188 6099
Size: 440 911
Fragments arranged by size:
1928 911 904 882 440 296 258 191
148 141
PspXI vC'TCGA_Gb
Cuts at: 0 3475 5155 6099
Size: 3475 1680 944
Fragments arranged by size:
3475 1680 944
PstI C_TGCA'G
Cuts at: 0 194 233 557 752 989 1187 1298 1457
Size: 194 39 324 195 237 198 111 159
Cuts at: 1457 1730 1814 2006 2027 2120 2363 2444 2831
Size: 273 84 192 21 93 243 81 387
Cuts at: 2831 2969 3293 3653 3884 4064 4508 4745 4787
Size: 138 324 360 231 180 444 237 42
Cuts at: 4787 4835 4841 5150 5807 5918 6099
Size: 48 6 309 657 111 181
Fragments arranged by size:
657 444 387 360 324 324 309 273
243 237 237 231 198 195 194 192
181 180 159 138 111 111 93 84
81 48 42 39 21 6
PvuII CAG'CTG
Cuts at: 0 363 585 714 855 984 1131 1290 1815
Size: 363 222 129 141 129 147 159 525
Cuts at: 1815 2211 2619 2631 2760 2832 2901 3006 4233
Size: 396 408 12 129 72 69 105 1227
Cuts at: 4233 4323 4836 5145 6099
Size: 90 513 309 954
Fragments arranged by size:
1227 954 525 513 408 396 363 309
222 159 147 141 129 129 129 105
90 72 69 12
SacI G_AGCT'C
Cuts at: 0 2290 2468 2669 3839 4346 4913 5012 5156
Size: 2290 178 201 1170 507 567 99 144
Cuts at: 5156 6099
Size: 943
Fragments arranged by size:
2290 1170 943 567 507 201 178 144
99
SacII CC_GC'GG
Cuts at: 0 899 3129 4040 6099
Size: 899 2230 911 2059
Fragments arranged by size:
2230 2059 911 899
SalI G'TCGA_C
Cuts at: 0 448 6099
Size: 448 5651
SanDI GG'GwC_CC
Cuts at: 0 38 65 439 2892 3072 6099
Size: 38 27 374 2453 180 3027
Fragments arranged by size:
3027 2453 374 180 38 27
SapI GCTCTTCn'nnn_
Cuts at: 0 5891 6099
Size: 5891 208
SbfI CC_TGCA'GG
Cuts at: 0 2444 2969 3884 4508 5807 5918 6099
Size: 2444 525 915 624 1299 111 181
Fragments arranged by size:
2444 1299 915 624 525 181 111
ScaI AGT'ACT
Cuts at: 0 5137 5239 6099
Size: 5137 102 860
Fragments arranged by size:
5137 860 102
SexAI A'CCwGG_T
Cuts at: 0 152 4717 6099
Size: 152 4565 1382
Fragments arranged by size:
4565 1382 152
SfcI C'TryA_G
Cuts at: 0 190 229 553 748 985 1183 1294 1453
Size: 190 39 324 195 237 198 111 159
Cuts at: 1453 1726 1810 2002 2023 2116 2359 2440 2827
Size: 273 84 192 21 93 243 81 387
Cuts at: 2827 2965 3289 3346 3649 3880 4060 4504 4741
Size: 138 324 57 303 231 180 444 237
Cuts at: 4741 4783 4831 4837 5146 5803 5914 6099
Size: 42 48 6 309 657 111 185
Fragments arranged by size:
657 444 387 324 324 309 303 273
243 237 237 231 198 195 192 190
185 180 159 138 111 111 93 84
81 57 48 42 39 21 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2752 4651 5587 6099
Size: 2752 1899 936 512
Fragments arranged by size:
2752 1899 936 512
SfoI GGC'GCC
Cuts at: 0 1107 4207 4675 6099
Size: 1107 3100 468 1424
Fragments arranged by size:
3100 1424 1107 468
SmaI CCC'GGG
Cuts at: 0 2788 4657 4726 4753 5782 6099
Size: 2788 1869 69 27 1029 317
Fragments arranged by size:
2788 1869 1029 317 69 27
SmlI C'TyrA_G
Cuts at: 0 280 319 3187 3475 3637 4219 4329 4345
Size: 280 39 2868 288 162 582 110 16
Cuts at: 4345 5155 6099
Size: 810 944
Fragments arranged by size:
2868 944 810 582 288 280 162 110
39 16
SphI G_CATG'C
Cuts at: 0 138 2108 6099
Size: 138 1970 3991
Fragments arranged by size:
3991 1970 138
StuI AGG'CCT
Cuts at: 0 2092 2587 5104 5704 6099
Size: 2092 495 2517 600 395
Fragments arranged by size:
2517 2092 600 495 395
StyI C'CwwG_G
Cuts at: 0 44 59 1673 1892 2849 3047 3395 3917
Size: 44 15 1614 219 957 198 348 522
Cuts at: 3917 4991 5960 5987 6099
Size: 1074 969 27 112
Fragments arranged by size:
1614 1074 969 957 522 348 219 198
112 44 27 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 3073 4147 6099
Size: 121 2952 1074 1952
Fragments arranged by size:
2952 1952 1074 121
TatI w'GTAC_w
Cuts at: 0 683 1076 3536 3746 5135 5237 5294 6099
Size: 683 393 2460 210 1389 102 57 805
Fragments arranged by size:
2460 1389 805 683 393 210 102 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3841 3873 5143 5175 5877 5909 6099
Size: 3841 32 1270 32 702 32 190
Fragments arranged by size:
3841 1270 702 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1030 1936 4588 5725 6099
Size: 1030 906 2652 1137 374
Fragments arranged by size:
2652 1137 1030 906 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 1791 1899 5544 6099
Size: 111 1680 108 3645 555
Fragments arranged by size:
3645 1680 555 111 108
XhoI C'TCGA_G
Cuts at: 0 3475 4345 5155 6099
Size: 3475 870 810 944
Fragments arranged by size:
3475 944 870 810
XmaI C'CCGG_G
Cuts at: 0 2786 4655 4724 4751 5780 6099
Size: 2786 1869 69 27 1029 319
Fragments arranged by size:
2786 1869 1029 319 69 27
XmnI GAAnn'nnTTC
Cuts at: 0 1846 2125 5704 6099
Size: 1846 279 3579 395
Fragments arranged by size:
3579 1846 395 279
ZraI GAC'GTC
Cuts at: 0 4521 6099
Size: 4521 1578
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI ApoI AscI AseI AsiSI
AvrII BciVI BfrBI BmtI BsiWI BsmI BspHI BsrDI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI