Restriction map for
pFN21AB3462
(Circular) (Six-base) MAPSORT of: pFN21AB3462.seq Check: 3706 from: 1 to: 10804
pFN21AB3462 10804 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3625 4317 7323 7801 7815 3625
Size: 692 3006 478 14 6614
Fragments arranged by size:
6614 3006 692 478 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6528 8614 278
Size: 53 83 186 936 4992 2086 2468
Fragments arranged by size:
4992 2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 8147 1937
Size: 517 5693 4594
Fragments arranged by size:
5693 4594 517
Acc65I G'GTAC_C
Cuts at: 8055 8125 8055
Size: 70 10734
AclI AA'CG_TT
Cuts at: 1563 8931 9304 1563
Size: 7368 373 3063
Fragments arranged by size:
7368 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 2669 2957 3488 3521 4112 4775 5192
Size: 653 384 288 531 33 591 663 417
Cuts at: 5192 5843 8867 10251 10519 1632
Size: 651 3024 1384 268 1917
Fragments arranged by size:
3024 1917 1384 663 653 651 591 531
417 384 288 268 33
AfeI AGC'GCT
Cuts at: 4455 7631 4455
Size: 3176 7628
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10607
AflIII A'CryG_T
Cuts at: 1203 2464 7092 9703 10680 1203
Size: 1261 4628 2611 977 1327
Fragments arranged by size:
4628 2611 1327 1261 977
AgeI A'CCGG_T
Cuts at: 9635 9635
Size: 10804
AhdI GACnn_n'nnGTC
Cuts at: 2450 9533 2450
Size: 7083 3721
AleI CACnn'nnGTG
Cuts at: 1166 3968 5066 1166
Size: 2802 1098 6904
Fragments arranged by size:
6904 2802 1098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4340 6095 6524 1184
Size: 3156 1755 429 5464
Fragments arranged by size:
5464 3156 1755 429
AlwNI CAG_nnn'CTG
Cuts at: 2119 2233 2590 2677 3049 3250 3267 3298 3419
Size: 114 357 87 372 201 17 31 121
Cuts at: 3419 3466 3640 3730 3739 3871 4444 4624 4636
Size: 47 174 90 9 132 573 180 12
Cuts at: 4636 5287 6238 6799 6835 7690 7822 7918 8547
Size: 651 951 561 36 855 132 96 629
Cuts at: 8547 10119 2119
Size: 1572 2804
Fragments arranged by size:
2804 1572 951 855 651 629 573 561
372 357 201 180 174 132 132 121
114 96 90 87 47 36 31 17
12 9
ApaI G_GGCC'C
Cuts at: 2913 3104 3362 3658 3806 5734 6616 6757 7197
Size: 191 258 296 148 1928 882 141 440
Cuts at: 7197 2913
Size: 6520
Fragments arranged by size:
6520 1928 882 440 296 258 191 148
141
ApaLI G'TGCA_C
Cuts at: 7640 8861 10017 7640
Size: 1221 1156 8427
Fragments arranged by size:
8427 1221 1156
ApoI r'AATT_y
Cuts at: 1493 8113 8339 8491 10600 1493
Size: 6620 226 152 2109 1697
Fragments arranged by size:
6620 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9358 160
Size: 872 8326 1606
Fragments arranged by size:
8326 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10804
AvaI C'yCGr_G
Cuts at: 1870 1943 4536 4791 5480 5795 6101 6350 6660
Size: 73 2593 255 689 315 306 249 310
Cuts at: 6660 6729 6756 7076 7160 7189 7457 7785 8129
Size: 69 27 320 84 29 268 328 344
Cuts at: 8129 1870
Size: 4545
Fragments arranged by size:
4545 2593 689 344 328 320 315 310
306 268 255 249 84 73 69 29
27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2953 2986 2953
Size: 33 10771
BamHI G'GATC_C
Cuts at: 5112 5611 6339 8134 8501 5112
Size: 499 728 1795 367 7415
Fragments arranged by size:
7415 1795 728 499 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2895 3110 6210 6678 8055
Size: 325 217 627 1108 215 3100 468 1377
Cuts at: 8055 8125 9580 618
Size: 70 1455 1842
Fragments arranged by size:
3100 1842 1455 1377 1108 627 468 325
217 215 70
BanII G_rGCy'C
Cuts at: 1391 2057 2913 3104 3362 3658 3806 4006 4183
Size: 666 856 191 258 296 148 200 177
Cuts at: 4183 4295 4473 4506 4674 4715 4724 5400 5448
Size: 112 178 33 168 41 9 676 48
Cuts at: 5448 5734 5795 5844 6351 6616 6757 6808 6918
Size: 286 61 49 507 265 141 51 110
Cuts at: 6918 7017 7161 7197 7464 7836 8123 1391
Size: 99 144 36 267 372 287 4072
Fragments arranged by size:
4072 856 676 666 507 372 296 287
286 267 265 258 200 191 178 177
168 148 144 141 112 110 99 61
51 49 48 41 36 33 9
BbeI G_GCGC'C
Cuts at: 3114 6214 6682 3114
Size: 3100 468 7236
Fragments arranged by size:
7236 3100 468
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5033 5373 6476 6560 6878 928
Size: 971 3134 340 1103 84 318 4854
Fragments arranged by size:
4854 3134 1103 971 340 318 84
BbvCI CC'TCA_GC
Cuts at: 2274 2340 3240 4838 5468 2274
Size: 66 900 1598 630 7610
Fragments arranged by size:
7610 1598 900 630 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4706 4740 8995 9029 1903
Size: 34 2769 34 4255 34 3678
Fragments arranged by size:
4255 3678 2769 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8696 9906 8696
Size: 1210 9594
BclI T'GATC_A
Cuts at: 1548 7893 1548
Size: 6345 4459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3108 3714 4448
Size: 107 122 71 631 605 1436 606 734
Cuts at: 4448 4757 6656 7415 7592 7706 9415 136
Size: 309 1899 759 177 114 1709 1525
Fragments arranged by size:
1899 1709 1525 1436 759 734 631 606
605 309 177 122 114 107 71
BglII A'GATC_T
Cuts at: 5598 6957 7059 10799 5598
Size: 1359 102 3740 5603
Fragments arranged by size:
5603 3740 1359 102
BlpI GC'TnA_GC
Cuts at: 2018 2720 2733 3662 5522 5829 6176 6272 6863
Size: 702 13 929 1860 307 347 96 591
Cuts at: 6863 7016 7619 7976 8221 2018
Size: 153 603 357 245 4601
Fragments arranged by size:
4601 1860 929 702 603 591 357 347
307 245 153 96 13
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2464 2913 3104 3362 3658 3719 3806
Size: 620 54 449 191 258 296 61 87
Cuts at: 3806 5734 6616 6757 7197 7644 8865 10021 1790
Size: 1928 882 141 440 447 1221 1156 2573
Fragments arranged by size:
2573 1928 1221 1156 882 620 449 447
440 296 258 191 141 87 61 54
BmgBI CAC'GTC
Cuts at: 1318 2305 2440 3862 5212 1318
Size: 987 135 1422 1350 6910
Fragments arranged by size:
6910 1422 1350 987 135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2467 3189 3283
Size: 416 53 83 399 24 1050 722 94
Cuts at: 3283 7180 7575 8535 9493 442
Size: 3897 395 960 958 1753
Fragments arranged by size:
3897 1753 1050 960 958 722 416 399
395 94 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10804
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3049 7651 7693 3049
Size: 4602 42 6160
Fragments arranged by size:
6160 4602 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2492 2549 2684 2785 2882 3158 3935
Size: 959 294 57 135 101 97 276 777
Cuts at: 3935 4322 4442 4457 4613 4658 4679 4829 4886
Size: 387 120 15 156 45 21 150 57
Cuts at: 4886 4928 5393 5435 5446 5564 5738 6002 6107
Size: 42 465 42 11 118 174 264 105
Cuts at: 6107 6428 6446 6515 6830 6947 7601 8069 9464
Size: 321 18 69 315 117 654 468 1395
Cuts at: 9464 1239
Size: 2579
Fragments arranged by size:
2579 1395 959 777 654 468 465 387
321 315 294 276 264 174 156 150
135 120 118 117 105 101 97 69
57 57 45 42 42 21 18 15
11
Bpu10I CC'TnA_GC
Cuts at: 2274 2340 3240 4289 4838 5108 5468 8018 2274
Size: 66 900 1049 549 270 360 2550 5060
Fragments arranged by size:
5060 2550 1049 900 549 360 270 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2309 5213 5627 6245 6355 8929 9794 10092
Size: 39 2904 414 618 110 2574 865 298
Cuts at: 10092 2270
Size: 2982
Fragments arranged by size:
2982 2904 2574 865 618 414 298 110
39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 2975 3365 3606 4525 5924 7019
Size: 617 1135 341 390 241 919 1399 1095
Cuts at: 7019 7034 7346 9467 882
Size: 15 312 2121 2219
Fragments arranged by size:
2219 2121 1399 1135 1095 919 617 390
341 312 241 15
BsaAI yAC'GTr
Cuts at: 493 1204 2998 3256 8595 493
Size: 711 1794 258 5339 2702
Fragments arranged by size:
5339 2702 1794 711 258
BsaBI GATnn'nnATC
Cuts at: 5117 7064 10798 5117
Size: 1947 3734 5123
Fragments arranged by size:
5123 3734 1947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 2657 3111 6211
Size: 53 83 186 936 1041 83 454 3100
Cuts at: 6211 6525 6679 8611 8993 9628 275
Size: 314 154 1932 382 635 1451
Fragments arranged by size:
3100 1932 1451 1041 936 635 454 382
314 186 154 83 83 53
BsaWI w'CCGG_w
Cuts at: 2625 5614 9237 9635 9909 10056 10467 2625
Size: 2989 3623 398 274 147 411 2962
Fragments arranged by size:
3623 2989 2962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4277 4307 5846 5876 7148
Size: 30 165 30 2874 30 1539 30 1272
Cuts at: 7148 7178 7946 7976 1178
Size: 30 768 30 4006
Fragments arranged by size:
4006 2874 1539 1272 768 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 4787 4834 4883 4958 4961 5318 5348
Size: 1171 2430 47 49 75 3 357 30
Cuts at: 5348 5438 5468 5485 5498 5501 5689 5803 6106
Size: 90 30 17 13 3 188 114 303
Cuts at: 6106 6119 6176 6209 6269 6680 6683 6749 6863
Size: 13 57 33 60 411 3 66 114
Cuts at: 6863 7025 7190 7220 7301 7357 7871 1186
Size: 162 165 30 81 56 514 4119
Fragments arranged by size:
4119 2430 1171 514 411 357 303 188
165 162 114 114 90 81 75 66
60 57 56 49 47 33 30 30
30 17 13 13 3 3 3
BseYI C'CCAG_C
Cuts at: 2087 2985 3406 3530 3723 4331 4343 4761 4845
Size: 898 421 124 193 608 12 418 84
Cuts at: 4845 4902 5103 6712 6900 7346 7445 7911 10007
Size: 57 201 1609 188 446 99 466 2096
Cuts at: 10007 10503 2087
Size: 496 2388
Fragments arranged by size:
2388 2096 1609 898 608 496 466 446
421 418 201 193 188 124 99 84
57 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2692 3075 3236 3311 3374 3884
Size: 573 618 338 383 161 75 63 510
Cuts at: 3884 3895 3992 4259 4817 5027 5516 5594 6140
Size: 11 97 267 558 210 489 78 546
Cuts at: 6140 6251 6392 6602 7540 7835 1163
Size: 111 141 210 938 295 4132
Fragments arranged by size:
4132 938 618 573 558 546 510 489
383 338 295 267 210 210 161 141
111 97 78 75 63 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4192 5818 7536 8254 9015 10043
Size: 275 186 2191 1626 1718 718 761 1028
Cuts at: 10043 1540
Size: 2301
Fragments arranged by size:
2301 2191 1718 1626 1028 761 718 275
186
BsiHKAI G_wGCw'C
Cuts at: 2550 2814 3385 4107 4295 4308 4473 4674 5844
Size: 264 571 722 188 13 165 201 1170
Cuts at: 5844 6168 6351 6918 7017 7161 7218 7430 7644
Size: 324 183 567 99 144 57 212 214
Cuts at: 7644 7800 8865 8950 10021 2550
Size: 156 1065 85 1071 3333
Fragments arranged by size:
3333 1170 1071 1065 722 571 567 324
264 214 212 201 188 183 165 156
144 99 85 57 13
BsmI GAATG_Cn'
Cuts at: 8315 8408 8315
Size: 93 10711
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2243 6701 7073 2174
Size: 69 4458 372 5905
Fragments arranged by size:
5905 4458 372 69
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2410 2464 2550 2814 2913 3104
Size: 399 267 353 54 86 264 99 191
Cuts at: 3104 3362 3385 3658 3719 3806 4006 4107 4183
Size: 258 23 273 61 87 200 101 76
Cuts at: 4183 4295 4308 4473 4506 4674 4715 4724 5400
Size: 112 13 165 33 168 41 9 676
Cuts at: 5400 5448 5734 5795 5844 6168 6351 6616 6757
Size: 48 286 61 49 324 183 265 141
Cuts at: 6757 6808 6918 7017 7161 7197 7218 7430 7464
Size: 51 110 99 144 36 21 212 34
Cuts at: 7464 7644 7800 7836 8123 8865 8950 10021 1391
Size: 180 156 36 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 676 399 353 324 287
286 273 267 265 264 258 212 200
191 183 180 168 165 156 144 141
112 110 101 99 99 87 86 85
76 61 61 54 51 49 48 41
36 36 34 33 23 21 13 9
BspEI T'CCGG_A
Cuts at: 2625 5614 10467 2625
Size: 2989 4853 2962
Fragments arranged by size:
4853 2989 2962
BspHI T'CATG_A
Cuts at: 8693 10423 8693
Size: 1730 9074
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 3195 3625 3856 4073 4161 4230 4317
Size: 1635 716 430 231 217 88 69 87
Cuts at: 4317 5538 6502 6516 7323 7623 7801 7815 8159
Size: 1221 964 14 807 300 178 14 344
Cuts at: 8159 844
Size: 3489
Fragments arranged by size:
3489 1635 1221 964 807 716 430 344
300 231 217 178 88 87 69 14
14
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 2377 3310 3919 4408 5377 5679
Size: 429 344 210 933 609 489 969 302
Cuts at: 5679 5917 5998 6180 6610 6973 7233 7405 8022
Size: 238 81 182 430 363 260 172 617
Cuts at: 8022 8070 8691 1394
Size: 48 621 3507
Fragments arranged by size:
3507 969 933 621 617 609 489 430
429 363 344 302 260 238 210 182
172 81 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 9299 9473 65
Size: 1183 8051 174 1396
Fragments arranged by size:
8051 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 3012 6171 6204 6245 7269 7715
Size: 110 395 807 3159 33 41 1024 446
Cuts at: 7715 9448 9635 1700
Size: 1733 187 2869
Fragments arranged by size:
3159 2869 1733 1024 807 446 395 187
110 41 33
BsrGI T'GTAC_A
Cuts at: 96 2688 7299 96
Size: 2592 4611 3601
Fragments arranged by size:
4611 3601 2592
BssHII G'CGCG_C
Cuts at: 1925 4086 1925
Size: 2161 8643
BssSI C'ACGA_G
Cuts at: 2372 2600 2825 5594 5963 8864 9876 2372
Size: 228 225 2769 369 2901 1012 3300
Fragments arranged by size:
3300 2901 2769 1012 369 228 225
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 2830 3250 3298 4115 4624 6238 6799
Size: 1796 240 420 48 817 509 1614 561
Cuts at: 6799 6835 794
Size: 36 4763
Fragments arranged by size:
4763 1796 1614 817 561 509 420 240
48 36
BstBI TT'CG_AA
Cuts at: 9608 9608
Size: 10804
BstEII G'GTnAC_C
Cuts at: 1184 2380 5059 5761 6133 6478 7180 1184
Size: 1196 2679 702 372 345 702 4808
Fragments arranged by size:
4808 2679 1196 702 702 372 345
BstXI CCAn_nnnn'nTGG
Cuts at: 3514 3679 6779 3514
Size: 165 3100 7539
Fragments arranged by size:
7539 3100 165
BstYI r'GATC_y
Cuts at: 1972 3910 3924 4392 5112 5118 5598 5611 6339
Size: 1938 14 468 720 6 480 13 728
Cuts at: 6339 6957 7059 7491 8134 8501 8886 8903 10355
Size: 618 102 432 643 367 385 17 1452
Cuts at: 10355 10441 10453 10799 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1938 1452 728 720 643 618 480
468 432 385 367 346 102 86 17
14 13 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2901 3798 5131 5742 5854 6042 6306
Size: 552 1836 897 1333 611 112 188 264
Cuts at: 6306 6366 6435 7536 7992 513
Size: 60 69 1101 456 3325
Fragments arranged by size:
3325 1836 1333 1101 897 611 552 456
264 188 112 69 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7664 487
Size: 683 56 6438 3627
Fragments arranged by size:
6438 3627 683 56
BtsI GCAGTG_nn'
Cuts at: 4521 6833 8320 9114 9134 4521
Size: 2312 1487 794 20 6191
Fragments arranged by size:
6191 2312 1487 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10769
DraI TTT'AAA
Cuts at: 8108 8108
Size: 10804
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5720 9811 809
Size: 507 4404 4091 1802
Fragments arranged by size:
4404 4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2778 3159 3429
Size: 54 1425 50 275 18 948 381 270
Cuts at: 3429 3480 3561 5343 5511 6303 6933 7309 7533
Size: 51 81 1782 168 792 630 376 224
Cuts at: 7533 7539 7593 8251 9140 10769 8
Size: 6 54 658 889 1629 43
Fragments arranged by size:
1782 1629 1425 948 889 792 658 630
381 376 275 270 224 168 81 54
54 51 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 7533 8251 1537
Size: 275 5721 718 4090
Fragments arranged by size:
5721 4090 718 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 2300 3018 3528 4370 4764 5192 6026
Size: 830 117 718 510 842 394 428 834
Cuts at: 6026 7736 7896 8734 1353
Size: 1710 160 838 3423
Fragments arranged by size:
3423 1710 842 838 834 830 718 510
428 394 160 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 5345 5870 5993 8062 9391
Size: 1064 138 948 2808 525 123 2069 1329
Cuts at: 9391 9765 9911 387
Size: 374 146 1280
Fragments arranged by size:
2808 2069 1329 1280 1064 948 525 374
146 138 123
EcoICRI GAG'CTC
Cuts at: 4293 4471 4672 5842 6349 6916 7015 7159 4293
Size: 178 201 1170 507 567 99 144 7938
Fragments arranged by size:
7938 1170 567 507 201 178 144 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2285 2492 2549 2669 2684 2785
Size: 393 566 87 207 57 120 15 101
Cuts at: 2785 2882 2957 3158 3488 3521 3935 4112 4322
Size: 97 75 201 330 33 414 177 210
Cuts at: 4322 4442 4457 4613 4658 4679 4775 4829 4886
Size: 120 15 156 45 21 96 54 57
Cuts at: 4886 4928 5192 5393 5435 5446 5564 5738 5843
Size: 42 264 201 42 11 118 174 105
Cuts at: 5843 6002 6107 6428 6446 6515 6830 6947 7601
Size: 159 105 321 18 69 315 117 654
Cuts at: 7601 8069 8867 9464 10251 10519 1239
Size: 468 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 654 597 566 468 414
393 330 321 315 268 264 210 207
201 201 177 174 159 156 120 120
118 117 105 105 101 97 96 87
75 69 57 57 54 45 42 42
33 21 18 15 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2561 2648 2756 3971 4436 4586 4858 5480
Size: 294 87 108 1215 465 150 272 622
Cuts at: 5480 5483 6191 6461 7114 2267
Size: 3 708 270 653 5957
Fragments arranged by size:
5957 1215 708 653 622 465 294 272
270 150 108 87 3
EcoO109I rG'GnC_Cy
Cuts at: 2043 2070 2230 2290 2444 2909 2932 3100 3101
Size: 27 160 60 154 465 23 168 1
Cuts at: 3101 3184 3358 3359 3435 3463 3655 3803 3979
Size: 83 174 1 76 28 192 148 176
Cuts at: 3979 4441 4692 4750 4842 4897 5077 5437 5722
Size: 462 251 58 92 55 180 360 285
Cuts at: 5722 5971 6145 6265 6415 6454 6753 6985 7193
Size: 249 174 120 150 39 299 232 208
Cuts at: 7193 7194 7660 7726 7876 2043
Size: 1 466 66 150 4971
Fragments arranged by size:
4971 466 465 462 360 299 285 251
249 232 208 192 180 176 174 174
168 160 154 150 150 148 120 92
83 76 66 60 58 55 39 28
27 23 1 1 1
EcoRI G'AATT_C
Cuts at: 8113 8491 8113
Size: 378 10426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 10804
FspI TGC'GCA
Cuts at: 2497 9310 2497
Size: 6813 3991
HaeII r_GCGC'y
Cuts at: 1678 2727 2746 3114 3730 4036 4171 4457 5863
Size: 1049 19 368 616 306 135 286 1406
Cuts at: 5863 6079 6214 6682 7633 9951 1678
Size: 216 135 468 951 2318 2531
Fragments arranged by size:
2531 2318 1406 1049 951 616 468 368
306 286 216 135 135 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 3263 3295 7946
Size: 32 135 32 864 32 795 32 4651
Cuts at: 7946 7978 9445 9477 9519 9551 1373
Size: 32 1467 32 42 32 2626
Fragments arranged by size:
4651 2626 1467 864 795 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 5848 8148 8394 669
Size: 988 281 517 3393 2300 246 3079
Fragments arranged by size:
3393 3079 2300 988 517 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10804
HpaI GTT'AAC
Cuts at: 8394 8394
Size: 10804
KasI G'GCGC_C
Cuts at: 3110 6210 6678 3110
Size: 3100 468 7236
Fragments arranged by size:
7236 3100 468
KpnI G_GTAC'C
Cuts at: 8059 8129 8059
Size: 70 10734
MfeI C'AATT_G
Cuts at: 8403 8403
Size: 10804
MluI A'CGCG_T
Cuts at: 2464 10680 2464
Size: 8216 2588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3090 9918 10102 1305
Size: 1785 6828 184 2007
Fragments arranged by size:
6828 2007 1785 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3431 3482 3563 5345 5513
Size: 54 1425 343 1599 51 81 1782 168
Cuts at: 5513 7541 10
Size: 2028 3273
Fragments arranged by size:
3273 2028 1782 1599 1425 343 168 81
54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 3708 3968 5066 8762 9121 9280 518
Size: 648 2542 260 1098 3696 359 159 2042
Fragments arranged by size:
3696 2542 2042 1098 648 359 260 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2590 2719 2860 2903 2989 3136 3193
Size: 762 222 129 141 43 86 147 57
Cuts at: 3193 3295 3661 3809 3820 4039 4216 4491 4624
Size: 102 366 148 11 219 177 275 133
Cuts at: 4624 4636 4765 4837 4849 4906 5011 5133 6044
Size: 12 129 72 12 57 105 122 911
Cuts at: 6044 6238 6328 6841 6904 7150 7266 8001 8042
Size: 194 90 513 63 246 116 735 41
Cuts at: 8042 8061 8220 8897 10045 10290 1606
Size: 19 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 762 735 677 513 366
275 246 245 222 219 194 177 159
148 147 141 133 129 129 122 116
105 102 90 86 72 63 57 57
43 41 19 12 12 11
NaeI GCC'GGC
Cuts at: 6173 6206 6247 7271 6173
Size: 33 41 1024 9706
Fragments arranged by size:
9706 1024 41 33
NarI GG'CG_CC
Cuts at: 3111 6211 6679 3111
Size: 3100 468 7236
Fragments arranged by size:
7236 3100 468
NcoI C'CATG_G
Cuts at: 513 1065 7992 513
Size: 552 6927 3325
Fragments arranged by size:
6927 3325 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10804
NgoMIV G'CCGG_C
Cuts at: 6171 6204 6245 7269 6171
Size: 33 41 1024 9706
Fragments arranged by size:
9706 1024 41 33
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10804
NotI GC'GGCC_GC
Cuts at: 8251 8251
Size: 10804
NspI r_CATG'y
Cuts at: 1132 1229 2143 4113 7096 7552 8162 9707 1132
Size: 97 914 1970 2983 456 610 1545 2229
Fragments arranged by size:
2983 2229 1970 1545 914 610 456 97
PasI CC'CwG_GG
Cuts at: 1348 2032 2448 5441 1348
Size: 684 416 2993 6711
Fragments arranged by size:
6711 2993 684 416
PciI A'CATG_T
Cuts at: 7092 9703 7092
Size: 2611 8193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 3049 4636 5773 1108
Size: 1011 930 1587 1137 6139
Fragments arranged by size:
6139 1587 1137 1011 930
PfoI T'CCnGG_A
Cuts at: 2175 2298 3018 3927 4134 4458 5451 5580 6279
Size: 123 720 909 207 324 993 129 699
Cuts at: 6279 6342 6597 6981 7068 7218 7886 2175
Size: 63 255 384 87 150 668 5093
Fragments arranged by size:
5093 993 909 720 699 668 384 324
255 207 150 129 123 87 63
PmeI GTTT'AAAC
Cuts at: 8108 8108
Size: 10804
PmlI CAC'GTG
Cuts at: 2998 3256 2998
Size: 258 10546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8873 8905 10386 10418 8873
Size: 32 1481 32 9259
Fragments arranged by size:
9259 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 2070 2290 2444 3184 4692 4842 4897 5077
Size: 27 220 154 740 1508 150 55 180
Cuts at: 5077 5437 5722 5971 6145 6415 6454 6985 7726
Size: 360 285 249 174 270 39 531 741
Cuts at: 7726 2043
Size: 5121
Fragments arranged by size:
5121 1508 741 740 531 360 285 270
249 220 180 174 154 150 55 39
27
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10804
PsiI TTA'TAA
Cuts at: 8374 8374
Size: 10804
PspOMI G'GGCC_C
Cuts at: 2909 3100 3358 3654 3802 5730 6612 6753 7193
Size: 191 258 296 148 1928 882 141 440
Cuts at: 7193 2909
Size: 6520
Fragments arranged by size:
6520 1928 882 440 296 258 191 148
141
PspXI vC'TCGA_Gb
Cuts at: 5480 7160 5480
Size: 1680 9124
PstI C_TGCA'G
Cuts at: 830 2199 2238 2562 2757 2994 3192 3303 3462
Size: 1369 39 324 195 237 198 111 159
Cuts at: 3462 3735 3819 4011 4032 4125 4368 4449 4836
Size: 273 84 192 21 93 243 81 387
Cuts at: 4836 4974 5298 5658 5889 6069 6513 6750 6792
Size: 138 324 360 231 180 444 237 42
Cuts at: 6792 6840 6846 7155 7812 7923 8156 830
Size: 48 6 309 657 111 233 3478
Fragments arranged by size:
3478 1369 657 444 387 360 324 324
309 273 243 237 237 233 231 198
195 192 180 159 138 111 111 93
84 81 48 42 39 21 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10804
PvuII CAG'CTG
Cuts at: 2368 2590 2719 2860 2989 3136 3295 3820 4216
Size: 222 129 141 129 147 159 525 396
Cuts at: 4216 4624 4636 4765 4837 4906 5011 6238 6328
Size: 408 12 129 72 69 105 1227 90
Cuts at: 6328 6841 7150 2368
Size: 513 309 6022
Fragments arranged by size:
6022 1227 525 513 408 396 309 222
159 147 141 129 129 129 105 90
72 69 12
SacI G_AGCT'C
Cuts at: 4295 4473 4674 5844 6351 6918 7017 7161 4295
Size: 178 201 1170 507 567 99 144 7938
Fragments arranged by size:
7938 1170 567 507 201 178 144 99
SacII CC_GC'GG
Cuts at: 2904 5134 6045 2904
Size: 2230 911 7663
Fragments arranged by size:
7663 2230 911
SalI G'TCGA_C
Cuts at: 1936 2453 8146 1936
Size: 517 5693 4594
Fragments arranged by size:
5693 4594 517
SanDI GG'GwC_CC
Cuts at: 2043 2070 2444 4897 5077 2043
Size: 27 374 2453 180 7770
Fragments arranged by size:
7770 2453 374 180 27
SapI GCTCTTCn'nnn_
Cuts at: 7896 7896
Size: 10804
SbfI CC_TGCA'GG
Cuts at: 4449 4974 5889 6513 7812 7923 8156 4449
Size: 525 915 624 1299 111 233 7097
Fragments arranged by size:
7097 1299 915 624 525 233 111
ScaI AGT'ACT
Cuts at: 7142 7244 9052 10764 7142
Size: 102 1808 1712 7182
Fragments arranged by size:
7182 1808 1712 102
SexAI A'CCwGG_T
Cuts at: 2157 6722 2157
Size: 4565 6239
SfcI C'TryA_G
Cuts at: 826 1045 2195 2234 2558 2753 2990 3188 3299
Size: 219 1150 39 324 195 237 198 111
Cuts at: 3299 3458 3731 3815 4007 4028 4121 4364 4445
Size: 159 273 84 192 21 93 243 81
Cuts at: 4445 4832 4970 5294 5351 5654 5885 6065 6509
Size: 387 138 324 57 303 231 180 444
Cuts at: 6509 6746 6788 6836 6842 7151 7808 7919 8152
Size: 237 42 48 6 309 657 111 233
Cuts at: 8152 9287 9968 10159 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1150 1135 681 657 444 387 324
324 309 303 273 243 237 237 233
231 219 198 195 192 191 180 159
138 111 111 93 84 81 57 48
42 39 21 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 4757 6656 7592 4757
Size: 1899 936 7969
Fragments arranged by size:
7969 1899 936
SfoI GGC'GCC
Cuts at: 3112 6212 6680 3112
Size: 3100 468 7236
Fragments arranged by size:
7236 3100 468
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10804
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10804
SmaI CCC'GGG
Cuts at: 1872 4793 6662 6731 6758 7787 8131 1872
Size: 2921 1869 69 27 1029 344 4545
Fragments arranged by size:
4545 2921 1869 1029 344 69 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 2324 5192 5480 5642 6224
Size: 197 926 342 39 2868 288 162 582
Cuts at: 6224 6334 6350 7160 8908 9809 10071 820
Size: 110 16 810 1748 901 262 1553
Fragments arranged by size:
2868 1748 1553 926 901 810 582 342
288 262 197 162 110 39 16
SnaBI TAC'GTA
Cuts at: 493 8595 493
Size: 8102 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10804
SphI G_CATG'C
Cuts at: 1132 2143 4113 8162 1132
Size: 1011 1970 4049 3774
Fragments arranged by size:
4049 3774 1970 1011
SspI AAT'ATT
Cuts at: 5 52 8728 5
Size: 47 8676 2081
Fragments arranged by size:
8676 2081 47
StuI AGG'CCT
Cuts at: 1517 4097 4592 7109 7709 1517
Size: 2580 495 2517 600 4612
Fragments arranged by size:
4612 2580 2517 600 495
StyI C'CwwG_G
Cuts at: 513 1065 2049 2064 3678 3897 4854 5052 5400
Size: 552 984 15 1614 219 957 198 348
Cuts at: 5400 5922 6996 7965 7992 8243 513
Size: 522 1074 969 27 251 3074
Fragments arranged by size:
3074 1614 1074 984 969 957 552 522
348 251 219 198 27 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 5078 6152 8831 8848 9001 9186
Size: 1255 546 2952 1074 2679 17 153 185
Cuts at: 9186 9631 325
Size: 445 1498
Fragments arranged by size:
2952 2679 1498 1255 1074 546 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 3081 5541
Size: 274 80 33 51 1443 711 393 2460
Cuts at: 5541 5751 7140 7242 7299 9050 10762 96
Size: 210 1389 102 57 1751 1712 138
Fragments arranged by size:
2460 1751 1712 1443 1389 711 393 274
210 138 102 80 57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5846 5878 7148 7180 7882 7914 10764 10796 5846
Size: 32 1270 32 702 32 2850 32 5854
Fragments arranged by size:
5854 2850 1270 702 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3035 3941 6593 7730 1316
Size: 1719 906 2652 1137 4390
Fragments arranged by size:
4390 2652 1719 1137 906
XbaI T'CTAG_A
Cuts at: 8140 8140
Size: 10804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 3796 3904 7549 2116
Size: 1680 108 3645 5371
Fragments arranged by size:
5371 3645 1680 108
XhoI C'TCGA_G
Cuts at: 1943 5480 6350 7160 1943
Size: 3537 870 810 5587
Fragments arranged by size:
5587 3537 870 810
XmaI C'CCGG_G
Cuts at: 1870 4791 6660 6729 6756 7785 8129 1870
Size: 2921 1869 69 27 1029 344 4545
Fragments arranged by size:
4545 2921 1869 1029 344 69 27
XmnI GAAnn'nnTTC
Cuts at: 3851 4130 7709 8933 3851
Size: 279 3579 1224 5722
Fragments arranged by size:
5722 3579 1224 279
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6526 8612 276
Size: 53 83 186 936 4992 2086 2468
Fragments arranged by size:
4992 2468 2086 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII
SrfI SwaI