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SpecieshumanProduct IDFHC36555Cloning SiteSgfI-PmeIGenBank AccessionGeneKIEE3478SymbolLRP6
Alias : ADCAD2, STHAG7Descriptionlow density lipoprotein receptor-related protein 6Original Clone IDcp93478NoteWe obtained this clone using the E.coli strain DH10B at 25 deg C.
Characterization of the cloned ORF
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Length: 4839 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1613 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS8647.1 0 99.9 LRP6 CCDS8181.1 0 70.3 LRP5 CCDS31478.1 1.2e-106 47.1 LRP4 CCDS2182.1 2.3e-45 32.3 LRP1B CCDS33626.1 1.5e-34 62.6 LRP5L
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan NULL 1261 1282 PR00261 NULL NULL 1298 1319 PR00261 NULL NULL 1336 1357 PR00261 NULL HMMPfam IPR000033 108 147 PF00058 LDLR class B repeat IPR000033 150 191 PF00058 LDLR class B repeat IPR000033 194 233 PF00058 LDLR class B repeat IPR000033 372 413 PF00058 LDLR class B repeat IPR000033 415 455 PF00058 LDLR class B repeat IPR000033 458 499 PF00058 LDLR class B repeat IPR000033 674 714 PF00058 LDLR class B repeat IPR000033 718 757 PF00058 LDLR class B repeat IPR000033 760 800 PF00058 LDLR class B repeat IPR000033 843 882 PF00058 LDLR class B repeat IPR000033 1069 1110 PF00058 LDLR class B repeat IPR000033 1114 1154 PF00058 LDLR class B repeat IPR002172 1247 1285 PF00057 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1286 1322 PF00057 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1326 1360 PF00057 Low-density lipoprotein (LDL) receptor class A repeat HMMSmart IPR000033 43 85 SM00135 LDLR class B repeat IPR000033 87 129 SM00135 LDLR class B repeat IPR000033 130 173 SM00135 LDLR class B repeat IPR000033 174 216 SM00135 LDLR class B repeat IPR000033 217 258 SM00135 LDLR class B repeat IPR006210 285 324 SM00181 Epidermal growth factor-like IPR000033 352 394 SM00135 LDLR class B repeat IPR000033 395 437 SM00135 LDLR class B repeat IPR000033 438 481 SM00135 LDLR class B repeat IPR000033 482 524 SM00135 LDLR class B repeat IPR000033 525 565 SM00135 LDLR class B repeat IPR006210 591 628 SM00181 Epidermal growth factor-like IPR000033 654 696 SM00135 LDLR class B repeat IPR000033 697 739 SM00135 LDLR class B repeat IPR000033 740 783 SM00135 LDLR class B repeat IPR000033 784 825 SM00135 LDLR class B repeat IPR000033 827 865 SM00135 LDLR class B repeat IPR006210 892 930 SM00181 Epidermal growth factor-like IPR000033 957 999 SM00135 LDLR class B repeat IPR000033 1001 1048 SM00135 LDLR class B repeat IPR000033 1049 1093 SM00135 LDLR class B repeat IPR000033 1094 1136 SM00135 LDLR class B repeat IPR000033 1137 1177 SM00135 LDLR class B repeat IPR006210 1206 1250 SM00181 Epidermal growth factor-like IPR002172 1248 1287 SM00192 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1288 1324 SM00192 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1325 1362 SM00192 Low-density lipoprotein (LDL) receptor class A repeat ProfileScan IPR000033 63 106 PS51120 LDLR class B repeat IPR000033 107 149 PS51120 LDLR class B repeat IPR000033 150 193 PS51120 LDLR class B repeat IPR000033 194 236 PS51120 LDLR class B repeat IPR000033 372 414 PS51120 LDLR class B repeat IPR000033 415 457 PS51120 LDLR class B repeat IPR000033 458 501 PS51120 LDLR class B repeat IPR000033 502 544 PS51120 LDLR class B repeat IPR000033 545 584 PS51120 LDLR class B repeat IPR000033 674 716 PS51120 LDLR class B repeat IPR000033 717 759 PS51120 LDLR class B repeat IPR000033 760 802 PS51120 LDLR class B repeat IPR000033 803 839 PS51120 LDLR class B repeat IPR000033 843 885 PS51120 LDLR class B repeat IPR000033 977 1025 PS51120 LDLR class B repeat IPR000033 1026 1068 PS51120 LDLR class B repeat IPR000033 1069 1113 PS51120 LDLR class B repeat IPR000033 1114 1156 PS51120 LDLR class B repeat IPR000033 1157 1198 PS51120 LDLR class B repeat IPR002172 1248 1286 PS50068 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1287 1323 PS50068 Low-density lipoprotein (LDL) receptor class A repeat IPR002172 1325 1361 PS50068 Low-density lipoprotein (LDL) receptor class A repeat
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 AVLRSLLACSFCVLLRAAPLLLY 25 PRIMARY 23 2 1374 VGSVIGVIVTIFVSGTVYFICQR 1396 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_006719141 1613 603507,610947,616724 99.9 100.0 NP_002327 1613 603507,610947,616724 99.9 100.0 XP_011518973 1462 603507,610947,616724 99.9 90.6 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21AE3478 Download>KIEE3478 4839 bp ATGGGGGCCGTCCTGAGGAGCCTCCTGGCCTGCAGCTTCTGTGTGCTCCTGAGAGCGGCC CCTTTGTTGCTTTATGCAAACAGACGGGACTTGCGATTGGTTGATGCTACAAATGGCAAA GAGAATGCTACGATTGTAGTTGGAGGCTTGGAGGATGCAGCTGCGGTGGACTTTGTGTTT AGTCATGGCTTGATATACTGGAGTGATGTCAGCGAAGAAGCCATTAAACGAACAGAATTT AACAAAACTGAGAGTGTGCAGAATGTTGTTGTTTCTGGATTATTGTCCCCCGATGGGCTG GCATGTGATTGGCTTGGAGAAAAATTGTACTGGACAGATTCTGAAACTAATCGGATTGAA GTTTCTAATTTAGATGGATCTTTACGAAAAGTTTTATTTTGGCAAGAGTTGGATCAACCC AGAGCTATTGCCTTAGATCCTTCAAGTGGGTTCATGTACTGGACAGACTGGGGAGAAGTG CCAAAGATAGAACGTGCTGGAATGGATGGTTCAAGTCGCTTCATTATAATAAACAGTGAA ATTTACTGGCCAAATGGACTGACTTTGGATTATGAAGAACAAAAGCTTTATTGGGCAGAT GCAAAACTTAATTTCATCCACAAATCAAATCTGGATGGAACAAATCGGCAGGCAGTGGTT AAAGGTTCCCTTCCACATCCTTTTGCCTTGACGTTATTTGAGGACATATTGTACTGGACT GACTGGAGCACACACTCCATTTTGGCTTGCAACAAGTATACTGGTGAGGGTCTGCGTGAA ATCCATTCTGACATCTTCTCTCCCATGGATATACATGCCTTCAGCCAACAGAGGCAGCCA AATGCCACAAATCCATGTGGAATTGACAATGGGGGTTGTTCCCATTTGTGTTTGATGTCT CCAGTCAAGCCTTTTTATCAGTGTGCTTGCCCCACTGGGGTCAAACTCCTGGAGAATGGA AAAACCTGCAAAGATGGTGCCACAGAATTATTGCTTTTAGCTCGAAGGACAGACTTGAGA CGCATTTCTTTGGATACACCAGATTTTACAGACATTGTTCTGCAGTTAGAAGACATCCGT CATGCCATTGCCATAGATTACGATCCTGTGGAAGGCTACATCTACTGGACTGATGATGAA GTGAGGGCCATACGCCGTTCATTTATAGATGGATCTGGCAGTCAGTTTGTGGTCACTGCT CAAATTGCCCATCCTGATGGTATTGCTGTGGACTGGGTTGCACGAAATCTTTATTGGACA GACACTGGCACTGATCGAATAGAAGTGACAAGGCTCAATGGGACCATGAGGAAGATCTTG ATTTCAGAGGACTTAGAGGAACCCCGGGCTATTGTGTTAGATCCCATGGTTGGGTACATG TATTGGACTGACTGGGGAGAAATTCCGAAAATTGAGCGAGCAGCTCTGGATGGTTCTGAC CGTGTAGTATTGGTTAACACTTCTCTTGGTTGGCCAAATGGTTTAGCCTTGGATTATGAT GAAGGCAAAATATACTGGGGAGATGCCAAAACAGACAAGATTGAGGTTATGAATACTGAT GGCACTGGGAGACGAGTACTAGTGGAAGACAAAATTCCTCACATATTTGGATTTACTTTG TTGGGTGACTATGTTTACTGGACTGACTGGCAGAGGCGTAGCATTGAAAGAGTTCATAAA CGAAGTGCAGAGAGGGAAGTGATCATAGATCAGCTGCCTGACCTCATGGGCCTAAAGGCT ACAAATGTTCATCGAGTGATTGGTTCCAACCCCTGTGCTGAGGAAAACGGGGGATGTAGC CATCTCTGCCTCTATAGACCTCAGGGCCTTCGCTGTGCTTGCCCTATTGGCTTTGAACTC ATCAGTGACATGAAGACCTGCATTGTCCCAGAGGCTTTCCTTTTGTTTTCACGGAGAGCA GATATCAGACGAATTTCTCTGGAAACAAACAATAATAATGTGGCTATTCCACTCACTGGT GTCAAAGAAGCTTCTGCTTTGGATTTTGATGTGACAGACAACCGAATTTATTGGACTGAT ATATCACTCAAGACCATCAGCAGAGCCTTTATGAATGGCAGTGCACTGGAACATGTGGTA GAATTCGGCTTAGATTATCCAGAAGGCATGGCAGTAGACTGGCTTGGGAAGAACTTGTAC TGGGCAGACACAGGAACGAATCGAATTGAGGTGTCAAAGTTGGATGGGCAGCACCGACAA GTTTTGGTGTGGAAAGACCTAGATAGTCCCAGAGCTCTCGCGTTGGACCCTGCCGAAGGA TTTATGTATTGGACTGAATGGGGTGGAAAACCTAAGATAGACAGAGCTGCAATGGATGGA AGTGAACGTACTACCTTAGTTCCAAATGTGGGGCGGGCAAACGGCCTAACTATTGATTAT GCTAAAAGGAGGCTTTATTGGACAGACCTGGACACCAACTTAATAGAATCTTCAAATATG CTTGGGCTCAACCGTGAAGTTATAGCAGATGACTTGCCTCATCCTTTTGGCTTAACTCAG TACCAAGATTATATCTACTGGACGGACTGGAGCCGACGCAGCATTGAGCGTGCCAACAAA ACCAGTGGCCAAAACCGCACCATCATTCAGGGCCATTTGGATTATGTGATGGACATCCTC GTCTTTCACTCATCTCGACAGTCAGGGTGGAATGAATGTGCTTCCAGCAATGGGCACTGC TCCCACCTCTGCTTGGCTGTGCCAGTTGGGGGTTTTGTTTGTGGATGCCCTGCCCACTAC TCTCTTAATGCTGACAACAGGACTTGTAGTGCTCCTACGACTTTCCTGCTCTTCAGTCAA AAGAGTGCCATCAACCGCATGGTGATTGATGAACAACAGAGCCCCGACATCATCCTTCCC ATCCACAGCCTTCGGAATGTCCGGGCCATTGACTATGACCCACTGGACAAGCAACTCTAT TGGATTGACTCACGACAAAACATGATCCGAAAGGCACAAGAAGATGGCAGCCAGGGCTTT ACTGTGGTTGTGAGCTCAGTTCCGAGTCAGAACCTGGAAATACAACCCTATGACCTCAGC ATTGATATTTACAGCCGCTACATCTACTGGACTTGTGAGGCTACCAATGTCATTAATGTG ACAAGATTAGATGGGAGATCAGTTGGAGTGGTGCTGAAAGGCGAGCAGGACAGACCTCGA GCCATTGTGGTAAACCCAGAGAAAGGGTATATGTATTTTACCAATCTTCAGGAAAGGTCT CCTAAAATTGAACGGGCTGCTTTGGATGGGACAGAACGGGAGGTCCTCTTTTTCAGTGGC TTAAGTAAACCAATTGCTTTAGCCCTTGATAGCAGGCTGGGCAAGCTCTTTTGGGCTGAT TCAGATCTCCGGCGAATTGAAAGCAGTGATCTCTCAGGTGCTAACCGGATAGTATTAGAA GACTCCAATATCTTGCAGCCTGTGGGACTTACTGTGTTTGAAAACTGGCTCTATTGGATT GATAAACAGCAGCAAATGATTGAAAAAATTGACATGACAGGTCGAGAGGGTAGAACCAAA GTCCAAGCTCGAATTGCCCAGCTTAGTGACATTCATGCAGTAAAGGAGCTGAACCTTCAA GAATACAGACAGCACCCTTGTGCTCAGGATAATGGTGGCTGTTCACATATTTGTCTTGTA AAGGGGGATGGTACTACAAGGTGTTCTTGCCCCATGCACCTGGTTCTACTTCAAGATGAG CTATCATGTGGAGAACCTCCAACATGTTCTCCTCAGCAGTTTACTTGTTTCACGGGGGAA ATTGACTGTATCCCTGTGGCTTGGCGGTGCGATGGGTTTACTGAATGTGAAGACCACAGT GATGAACTCAATTGTCCTGTATGCTCAGAGTCCCAGTTCCAGTGTGCCAGTGGGCAGTGT ATTGATGGTGCCCTCCGATGCAATGGAGATGCAAACTGCCAGGACAAATCAGATGAGAAG AACTGTGAAGTGCTTTGTTTAATTGATCAGTTCCGCTGTGCCAATGGTCAGTGCATTGGA AAGCACAAGAAGTGTGATCATAATGTGGATTGCAGTGACAAGTCAGATGAACTGGATTGT TATCCGACTGAAGAACCAGCACCACAGGCCACCAATACAGTTGGTTCTGTTATTGGCGTA ATTGTCACCATTTTTGTGTCTGGAACTGTATACTTTATCTGCCAGAGGATGTTGTGTCCA CGTATGAAGGGAGATGGGGAAACTATGACTAATGACTATGTAGTTCATGGACCAGCTTCT GTGCCTCTTGGTTATGTGCCACACCCAAGTTCTTTGTCAGGATCTCTTCCAGGAATGTCT CGAGGTAAATCAATGATCAGCTCCCTCAGTATCATGGGGGGAAGCAGTGGACCCCCCTAT GACCGAGCCCATGTTACAGGAGCATCATCAAGTAGTTCTTCAAGCACCAAAGGCACTTAC TTCCCTGCAATTTTGAACCCTCCACCATCCCCAGCCACAGAGCGATCACATTACACTATG GAATTTGGATATTCTTCAAACAGTCCTTCCACTCATAGGTCATACAGCTACAGGCCATAT AGCTACCGGCACTTTGCACCCCCCACCACACCCTGCAGCACAGATGTTTGTGACAGTGAC TATGCTCCTAGTCGGAGAATGACCTCAGTGGCAACAGCCAAGGGCTATACCAGTGACTTG AACTATGATTCAGAACCTGTGCCCCCACCTCCCACACCCCGAAGCCAATACTTGTCAGCA GAGGAGAACTATGAAAGCTGCCCACCTTCTCCATACACAGAGAGGAGCTATTCTCATCAC CTCTACCCACCGCCACCCTCTCCCTGTACAGACTCCTCC
Cloned ORF protein sequence for pFN21AE3478 Download>KIEE3478 1613 aa MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVF SHGLIYWSDVSEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIE VSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSE IYWPNGLTLDYEEQKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDILYWT DWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMS PVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIR HAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWT DTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSD RVVLVNTSLGWPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTL LGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCS HLCLYRPQGLRCACPIGFELISDMKTCIVPEAFLLFSRRADIRRISLETNNNNVAIPLTG VKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLY WADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDG SERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQ YQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHC SHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILP IHSLRNVRAIDYDPLDKQLYWIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLS IDIYSRYIYWTCEATNVINVTRLDGRSVGVVLKGEQDRPRAIVVNPEKGYMYFTNLQERS PKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWADSDLRRIESSDLSGANRIVLE DSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQ EYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGE IDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEK NCEVLCLIDQFRCANGQCIGKHKKCDHNVDCSDKSDELDCYPTEEPAPQATNTVGSVIGV IVTIFVSGTVYFICQRMLCPRMKGDGETMTNDYVVHGPASVPLGYVPHPSSLSGSLPGMS RGKSMISSLSIMGGSSGPPYDRAHVTGASSSSSSSTKGTYFPAILNPPPSPATERSHYTM EFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSDYAPSRRMTSVATAKGYTSDL NYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCTDSS
Nucleotide Sequence (with vector) for pFN21AE3478 Download>pFN21AE3478 9544 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCCATGGGGGCCGTCCTGAGGAGCCTCCTGGCCTGCAG CTTCTGTGTGCTCCTGAGAGCGGCCCCTTTGTTGCTTTATGCAAACAGACGGGACTTGCG ATTGGTTGATGCTACAAATGGCAAAGAGAATGCTACGATTGTAGTTGGAGGCTTGGAGGA TGCAGCTGCGGTGGACTTTGTGTTTAGTCATGGCTTGATATACTGGAGTGATGTCAGCGA AGAAGCCATTAAACGAACAGAATTTAACAAAACTGAGAGTGTGCAGAATGTTGTTGTTTC TGGATTATTGTCCCCCGATGGGCTGGCATGTGATTGGCTTGGAGAAAAATTGTACTGGAC AGATTCTGAAACTAATCGGATTGAAGTTTCTAATTTAGATGGATCTTTACGAAAAGTTTT ATTTTGGCAAGAGTTGGATCAACCCAGAGCTATTGCCTTAGATCCTTCAAGTGGGTTCAT GTACTGGACAGACTGGGGAGAAGTGCCAAAGATAGAACGTGCTGGAATGGATGGTTCAAG TCGCTTCATTATAATAAACAGTGAAATTTACTGGCCAAATGGACTGACTTTGGATTATGA AGAACAAAAGCTTTATTGGGCAGATGCAAAACTTAATTTCATCCACAAATCAAATCTGGA TGGAACAAATCGGCAGGCAGTGGTTAAAGGTTCCCTTCCACATCCTTTTGCCTTGACGTT ATTTGAGGACATATTGTACTGGACTGACTGGAGCACACACTCCATTTTGGCTTGCAACAA GTATACTGGTGAGGGTCTGCGTGAAATCCATTCTGACATCTTCTCTCCCATGGATATACA TGCCTTCAGCCAACAGAGGCAGCCAAATGCCACAAATCCATGTGGAATTGACAATGGGGG TTGTTCCCATTTGTGTTTGATGTCTCCAGTCAAGCCTTTTTATCAGTGTGCTTGCCCCAC TGGGGTCAAACTCCTGGAGAATGGAAAAACCTGCAAAGATGGTGCCACAGAATTATTGCT TTTAGCTCGAAGGACAGACTTGAGACGCATTTCTTTGGATACACCAGATTTTACAGACAT TGTTCTGCAGTTAGAAGACATCCGTCATGCCATTGCCATAGATTACGATCCTGTGGAAGG CTACATCTACTGGACTGATGATGAAGTGAGGGCCATACGCCGTTCATTTATAGATGGATC TGGCAGTCAGTTTGTGGTCACTGCTCAAATTGCCCATCCTGATGGTATTGCTGTGGACTG GGTTGCACGAAATCTTTATTGGACAGACACTGGCACTGATCGAATAGAAGTGACAAGGCT CAATGGGACCATGAGGAAGATCTTGATTTCAGAGGACTTAGAGGAACCCCGGGCTATTGT GTTAGATCCCATGGTTGGGTACATGTATTGGACTGACTGGGGAGAAATTCCGAAAATTGA GCGAGCAGCTCTGGATGGTTCTGACCGTGTAGTATTGGTTAACACTTCTCTTGGTTGGCC AAATGGTTTAGCCTTGGATTATGATGAAGGCAAAATATACTGGGGAGATGCCAAAACAGA CAAGATTGAGGTTATGAATACTGATGGCACTGGGAGACGAGTACTAGTGGAAGACAAAAT TCCTCACATATTTGGATTTACTTTGTTGGGTGACTATGTTTACTGGACTGACTGGCAGAG GCGTAGCATTGAAAGAGTTCATAAACGAAGTGCAGAGAGGGAAGTGATCATAGATCAGCT GCCTGACCTCATGGGCCTAAAGGCTACAAATGTTCATCGAGTGATTGGTTCCAACCCCTG TGCTGAGGAAAACGGGGGATGTAGCCATCTCTGCCTCTATAGACCTCAGGGCCTTCGCTG TGCTTGCCCTATTGGCTTTGAACTCATCAGTGACATGAAGACCTGCATTGTCCCAGAGGC TTTCCTTTTGTTTTCACGGAGAGCAGATATCAGACGAATTTCTCTGGAAACAAACAATAA TAATGTGGCTATTCCACTCACTGGTGTCAAAGAAGCTTCTGCTTTGGATTTTGATGTGAC AGACAACCGAATTTATTGGACTGATATATCACTCAAGACCATCAGCAGAGCCTTTATGAA TGGCAGTGCACTGGAACATGTGGTAGAATTCGGCTTAGATTATCCAGAAGGCATGGCAGT AGACTGGCTTGGGAAGAACTTGTACTGGGCAGACACAGGAACGAATCGAATTGAGGTGTC AAAGTTGGATGGGCAGCACCGACAAGTTTTGGTGTGGAAAGACCTAGATAGTCCCAGAGC TCTCGCGTTGGACCCTGCCGAAGGATTTATGTATTGGACTGAATGGGGTGGAAAACCTAA GATAGACAGAGCTGCAATGGATGGAAGTGAACGTACTACCTTAGTTCCAAATGTGGGGCG GGCAAACGGCCTAACTATTGATTATGCTAAAAGGAGGCTTTATTGGACAGACCTGGACAC CAACTTAATAGAATCTTCAAATATGCTTGGGCTCAACCGTGAAGTTATAGCAGATGACTT GCCTCATCCTTTTGGCTTAACTCAGTACCAAGATTATATCTACTGGACGGACTGGAGCCG ACGCAGCATTGAGCGTGCCAACAAAACCAGTGGCCAAAACCGCACCATCATTCAGGGCCA TTTGGATTATGTGATGGACATCCTCGTCTTTCACTCATCTCGACAGTCAGGGTGGAATGA ATGTGCTTCCAGCAATGGGCACTGCTCCCACCTCTGCTTGGCTGTGCCAGTTGGGGGTTT TGTTTGTGGATGCCCTGCCCACTACTCTCTTAATGCTGACAACAGGACTTGTAGTGCTCC TACGACTTTCCTGCTCTTCAGTCAAAAGAGTGCCATCAACCGCATGGTGATTGATGAACA ACAGAGCCCCGACATCATCCTTCCCATCCACAGCCTTCGGAATGTCCGGGCCATTGACTA TGACCCACTGGACAAGCAACTCTATTGGATTGACTCACGACAAAACATGATCCGAAAGGC ACAAGAAGATGGCAGCCAGGGCTTTACTGTGGTTGTGAGCTCAGTTCCGAGTCAGAACCT GGAAATACAACCCTATGACCTCAGCATTGATATTTACAGCCGCTACATCTACTGGACTTG TGAGGCTACCAATGTCATTAATGTGACAAGATTAGATGGGAGATCAGTTGGAGTGGTGCT GAAAGGCGAGCAGGACAGACCTCGAGCCATTGTGGTAAACCCAGAGAAAGGGTATATGTA TTTTACCAATCTTCAGGAAAGGTCTCCTAAAATTGAACGGGCTGCTTTGGATGGGACAGA ACGGGAGGTCCTCTTTTTCAGTGGCTTAAGTAAACCAATTGCTTTAGCCCTTGATAGCAG GCTGGGCAAGCTCTTTTGGGCTGATTCAGATCTCCGGCGAATTGAAAGCAGTGATCTCTC AGGTGCTAACCGGATAGTATTAGAAGACTCCAATATCTTGCAGCCTGTGGGACTTACTGT GTTTGAAAACTGGCTCTATTGGATTGATAAACAGCAGCAAATGATTGAAAAAATTGACAT GACAGGTCGAGAGGGTAGAACCAAAGTCCAAGCTCGAATTGCCCAGCTTAGTGACATTCA TGCAGTAAAGGAGCTGAACCTTCAAGAATACAGACAGCACCCTTGTGCTCAGGATAATGG TGGCTGTTCACATATTTGTCTTGTAAAGGGGGATGGTACTACAAGGTGTTCTTGCCCCAT GCACCTGGTTCTACTTCAAGATGAGCTATCATGTGGAGAACCTCCAACATGTTCTCCTCA GCAGTTTACTTGTTTCACGGGGGAAATTGACTGTATCCCTGTGGCTTGGCGGTGCGATGG GTTTACTGAATGTGAAGACCACAGTGATGAACTCAATTGTCCTGTATGCTCAGAGTCCCA GTTCCAGTGTGCCAGTGGGCAGTGTATTGATGGTGCCCTCCGATGCAATGGAGATGCAAA CTGCCAGGACAAATCAGATGAGAAGAACTGTGAAGTGCTTTGTTTAATTGATCAGTTCCG CTGTGCCAATGGTCAGTGCATTGGAAAGCACAAGAAGTGTGATCATAATGTGGATTGCAG TGACAAGTCAGATGAACTGGATTGTTATCCGACTGAAGAACCAGCACCACAGGCCACCAA TACAGTTGGTTCTGTTATTGGCGTAATTGTCACCATTTTTGTGTCTGGAACTGTATACTT TATCTGCCAGAGGATGTTGTGTCCACGTATGAAGGGAGATGGGGAAACTATGACTAATGA CTATGTAGTTCATGGACCAGCTTCTGTGCCTCTTGGTTATGTGCCACACCCAAGTTCTTT GTCAGGATCTCTTCCAGGAATGTCTCGAGGTAAATCAATGATCAGCTCCCTCAGTATCAT GGGGGGAAGCAGTGGACCCCCCTATGACCGAGCCCATGTTACAGGAGCATCATCAAGTAG TTCTTCAAGCACCAAAGGCACTTACTTCCCTGCAATTTTGAACCCTCCACCATCCCCAGC CACAGAGCGATCACATTACACTATGGAATTTGGATATTCTTCAAACAGTCCTTCCACTCA TAGGTCATACAGCTACAGGCCATATAGCTACCGGCACTTTGCACCCCCCACCACACCCTG CAGCACAGATGTTTGTGACAGTGACTATGCTCCTAGTCGGAGAATGACCTCAGTGGCAAC AGCCAAGGGCTATACCAGTGACTTGAACTATGATTCAGAACCTGTGCCCCCACCTCCCAC ACCCCGAAGCCAATACTTGTCAGCAGAGGAGAACTATGAAAGCTGCCCACCTTCTCCATA CACAGAGAGGAGCTATTCTCATCACCTCTACCCACCGCCACCCTCTCCCTGTACAGACTC CTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG ATCT
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