(Circular) (Six-base) MAPSORT of: pFN21AE3478.seq Check: 773 from: 1 to: 9544 pFN21AE3478 9544 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7354 278 Size: 53 83 186 936 5818 2468 Fragments arranged by size: 5818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2762 4140 6174 6887 1937 Size: 825 1378 2034 713 4594 Fragments arranged by size: 4594 2034 1378 825 713 Acc65I G'GTAC_C Cuts at: 6865 6865 Size: 9544 AclI AA'CG_TT Cuts at: 1563 7671 8044 1563 Size: 6108 373 3063 Fragments arranged by size: 6108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2809 4801 5216 6114 7607 8991 9259 1632 Size: 1177 1992 415 898 1493 1384 268 1917 Fragments arranged by size: 1992 1917 1493 1384 1177 898 415 268 AflII C'TTAA_G Cuts at: 820 1017 5305 820 Size: 197 4288 5059 Fragments arranged by size: 5059 4288 197 AflIII A'CryG_T Cuts at: 1203 3381 4096 5747 8443 9420 1203 Size: 2178 715 1651 2696 977 1327 Fragments arranged by size: 2696 2178 1651 1327 977 715 AgeI A'CCGG_T Cuts at: 8375 8375 Size: 9544 AhdI GACnn_n'nnGTC Cuts at: 8273 8273 Size: 9544 AleI CACnn'nnGTG Cuts at: 1166 5622 1166 Size: 4456 5088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2862 5640 1184 Size: 1678 2778 5088 Fragments arranged by size: 5088 2778 1678 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5271 5303 5271 Size: 32 9512 AlwNI CAG_nnn'CTG Cuts at: 2043 2345 3269 5038 6263 6701 7287 8859 2043 Size: 302 924 1769 1225 438 586 1572 2728 Fragments arranged by size: 2728 1769 1572 1225 924 586 438 302 ApaLI G'TGCA_C Cuts at: 4086 7601 8757 4086 Size: 3515 1156 4873 Fragments arranged by size: 4873 3515 1156 ApoI r'AATT_y Cuts at: 1493 2240 2544 3405 3597 3936 4029 4106 6506 Size: 747 304 861 192 339 93 77 2400 Cuts at: 6506 6853 7079 7231 9340 1493 Size: 347 226 152 2109 1697 Fragments arranged by size: 2400 2109 1697 861 747 347 339 304 226 192 152 93 77 AseI AT'TA_AT Cuts at: 160 1032 5118 8098 160 Size: 872 4086 2980 1606 Fragments arranged by size: 4086 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9544 AvaI C'yCGr_G Cuts at: 1870 1943 3348 5181 6324 6869 1870 Size: 73 1405 1833 1143 545 4545 Fragments arranged by size: 4545 1833 1405 1143 545 73 BamHI G'GATC_C Cuts at: 6874 7241 6874 Size: 367 9177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2981 5912 6865 8320 618 Size: 325 217 627 1194 2931 953 1455 1842 Fragments arranged by size: 2931 1842 1455 1194 953 627 325 217 BanII G_rGCy'C Cuts at: 1391 4261 4473 4868 5021 6394 6863 1391 Size: 2870 212 395 153 1373 469 4072 Fragments arranged by size: 4072 2870 1373 469 395 212 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3081 3597 3884 5430 5841 928 Size: 971 1182 516 287 1546 411 4631 Fragments arranged by size: 4631 1546 1182 971 516 411 287 BbvCI CC'TCA_GC Cuts at: 3783 5060 5757 3783 Size: 1277 697 7570 Fragments arranged by size: 7570 1277 697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4792 4826 7735 7769 1903 Size: 34 2855 34 2909 34 3678 Fragments arranged by size: 3678 2909 2855 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3031 5804 7436 8646 3031 Size: 2773 1632 1210 3929 Fragments arranged by size: 3929 2773 1632 1210 BclI T'GATC_A Cuts at: 1548 3705 5989 6040 6339 1548 Size: 2157 2284 51 299 4753 Fragments arranged by size: 4753 2284 2157 299 51 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3852 8155 Size: 107 122 71 631 605 337 1843 4303 Cuts at: 8155 136 Size: 1525 Fragments arranged by size: 4303 1843 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3318 5368 9539 3318 Size: 2050 4171 3323 Fragments arranged by size: 4171 3323 2050 BlpI GC'TnA_GC Cuts at: 6961 6961 Size: 9544 Bme1580I G_kGCm'C Cuts at: 1790 4090 4701 5917 6708 7605 8761 1790 Size: 2300 611 1216 791 897 1156 2573 Fragments arranged by size: 2573 2300 1216 1156 897 791 611 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2482 2949 3247 Size: 416 53 83 399 24 1065 467 298 Cuts at: 3247 3406 3529 3579 4174 5872 7275 8233 442 Size: 159 123 50 595 1698 1403 958 1753 Fragments arranged by size: 1753 1698 1403 1065 958 595 467 416 399 298 159 123 83 53 50 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9544 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6813 6813 Size: 9544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2224 2749 2889 2975 4573 8204 1239 Size: 985 525 140 86 1598 3631 2579 Fragments arranged by size: 3631 2579 1598 985 525 140 86 Bpu10I CC'TnA_GC Cuts at: 3783 5060 5628 5757 3783 Size: 1277 568 129 7570 Fragments arranged by size: 7570 1277 568 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3040 4037 7669 8534 8832 3040 Size: 997 3632 865 298 3752 Fragments arranged by size: 3752 3632 997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5247 8207 882 Size: 617 3748 2960 2219 Fragments arranged by size: 3748 2960 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6206 7335 493 Size: 711 5002 1129 2702 Fragments arranged by size: 5002 2702 1129 711 BsaBI GATnn'nnATC Cuts at: 3105 4637 5367 6084 9538 3105 Size: 1532 730 717 3454 3111 Fragments arranged by size: 3454 3111 1532 730 717 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7351 7733 8368 275 Size: 53 83 186 936 5818 382 635 1451 Fragments arranged by size: 5818 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5409 7977 8375 8649 8796 9207 5409 Size: 2568 398 274 147 411 5746 Fragments arranged by size: 5746 2568 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5737 5767 1178 Size: 30 165 30 4334 30 4955 Fragments arranged by size: 4955 4334 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 5745 6761 6802 6829 1186 Size: 849 3710 1016 41 27 3901 Fragments arranged by size: 3901 3710 1016 849 41 27 BseYI C'CCAG_C Cuts at: 5341 5562 6475 8747 9243 5341 Size: 221 913 2272 496 5642 Fragments arranged by size: 5642 2272 913 496 221 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 3711 1163 Size: 573 546 1429 6996 Fragments arranged by size: 6996 1429 573 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6994 7755 8783 1540 Size: 275 186 4993 761 1028 2301 Fragments arranged by size: 4993 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2735 4090 4261 4798 5021 5629 7605 7690 Size: 683 1355 171 537 223 608 1976 85 Cuts at: 7690 8761 2052 Size: 1071 2835 Fragments arranged by size: 2835 1976 1355 1071 683 608 537 223 171 85 BsmI GAATG_Cn' Cuts at: 2134 7055 7148 2134 Size: 4921 93 4530 Fragments arranged by size: 4921 4530 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3016 3568 3016 Size: 552 8992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2735 4090 4261 4473 4701 4798 Size: 399 262 683 1355 171 212 228 97 Cuts at: 4798 4868 5021 5629 5917 6394 6708 6863 7605 Size: 70 153 608 288 477 314 155 742 Cuts at: 7605 7690 8761 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1355 1071 742 683 608 477 399 314 288 262 228 212 171 155 153 97 85 70 BspEI T'CCGG_A Cuts at: 9207 9207 Size: 9544 BspHI T'CATG_A Cuts at: 7433 9163 7433 Size: 1730 7814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 3890 6899 844 Size: 2134 912 3009 3489 Fragments arranged by size: 3489 3009 2134 912 BsrBI CCG'CTC Cuts at: 1394 1823 2060 7431 1394 Size: 429 237 5371 3507 Fragments arranged by size: 5371 3507 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 3090 4341 4699 5932 8039 8213 65 Size: 1183 1842 1251 358 1233 2107 174 1396 Fragments arranged by size: 2107 1842 1396 1251 1233 1183 358 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6570 8188 8375 1700 Size: 110 4760 1618 187 2869 Fragments arranged by size: 4760 2869 1618 187 110 BsrGI T'GTAC_A Cuts at: 96 6830 96 Size: 6734 2810 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9544 BssSI C'ACGA_G Cuts at: 7604 8616 7604 Size: 1012 8532 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9544 BstBI TT'CG_AA Cuts at: 8348 8348 Size: 9544 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9544 BstXI CCAn_nnnn'nTGG Cuts at: 3221 5704 3221 Size: 2483 7061 BstYI r'GATC_y Cuts at: 1972 2381 2440 3176 3318 3364 5368 6305 6874 Size: 409 59 736 142 46 2004 937 569 Cuts at: 6874 7241 7626 7643 9095 9181 9193 9539 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2004 1977 1452 937 736 569 409 385 367 346 142 86 59 46 17 12 BstZ17I GTA'TAC Cuts at: 2763 6175 2763 Size: 3412 6132 Bsu36I CC'TnA_GG Cuts at: 2018 3825 2018 Size: 1807 7737 BtgI C'CryG_G Cuts at: 513 1065 2004 2808 3369 513 Size: 552 939 804 561 6688 Fragments arranged by size: 6688 939 804 561 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5829 487 Size: 683 56 4603 4202 Fragments arranged by size: 4603 4202 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3198 4090 4699 5395 5906 6064 6376 7060 Size: 534 892 609 696 511 158 312 684 Cuts at: 7060 7854 7874 2664 Size: 794 20 4334 Fragments arranged by size: 4334 892 794 696 684 609 534 511 312 158 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4570 4605 529 Size: 35 4006 35 5468 Fragments arranged by size: 5468 4006 35 35 DraI TTT'AAA Cuts at: 6848 6848 Size: 9544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4643 8551 809 Size: 507 3327 3908 1802 Fragments arranged by size: 3908 3327 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2552 3476 4591 Size: 54 1425 50 275 18 722 924 1115 Cuts at: 4591 6991 7880 9509 8 Size: 2400 889 1629 43 Fragments arranged by size: 2400 1629 1425 1115 924 889 722 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6991 1537 Size: 275 5179 4090 Fragments arranged by size: 5179 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4820 6315 7474 1353 Size: 3467 1495 1159 3423 Fragments arranged by size: 3467 3423 1495 1159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8131 8505 8651 387 Size: 1064 138 6542 374 146 1280 Fragments arranged by size: 6542 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4259 5019 4259 Size: 760 8784 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2749 2809 2889 2975 4573 4801 Size: 393 592 525 60 80 86 1598 228 Cuts at: 4801 5216 6114 7607 8204 8991 9259 1239 Size: 415 898 1493 597 787 268 1524 Fragments arranged by size: 1598 1524 1493 898 787 597 592 525 415 393 268 228 86 80 60 EcoNI CCTnn'n_nnAGG Cuts at: 3114 4278 3114 Size: 1164 8380 EcoO109I rG'GnC_Cy Cuts at: 3829 5287 3829 Size: 1458 8086 EcoRI G'AATT_C Cuts at: 4106 6853 7231 4106 Size: 2747 378 6419 Fragments arranged by size: 6419 2747 378 EcoRV GAT'ATC Cuts at: 3928 3928 Size: 9544 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2690 5706 6438 2690 Size: 3016 732 5796 Fragments arranged by size: 5796 3016 732 FspI TGC'GCA Cuts at: 8050 8050 Size: 9544 HaeII r_GCGC'y Cuts at: 1678 8691 1678 Size: 7013 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 3697 3729 3912 Size: 32 135 32 1214 32 879 32 183 Cuts at: 3912 3944 5126 5158 5711 5743 6184 6216 8185 Size: 32 1182 32 553 32 441 32 1969 Cuts at: 8185 8217 8259 8291 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1969 1214 1182 879 553 441 183 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3460 6888 7134 669 Size: 988 281 1522 3428 246 3079 Fragments arranged by size: 3428 3079 1522 988 281 246 HindIII A'AGCT_T Cuts at: 748 2588 3993 748 Size: 1840 1405 6299 Fragments arranged by size: 6299 1840 1405 HpaI GTT'AAC Cuts at: 3460 7134 3460 Size: 3674 5870 KpnI G_GTAC'C Cuts at: 6869 6869 Size: 9544 MfeI C'AATT_G Cuts at: 5316 5854 7143 5316 Size: 538 1289 7717 Fragments arranged by size: 7717 1289 538 MluI A'CGCG_T Cuts at: 9420 9420 Size: 9544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2125 2394 3795 4185 4248 5128 5768 6113 Size: 820 269 1401 390 63 880 640 345 Cuts at: 6113 6617 8658 8842 1305 Size: 504 2041 184 2007 Fragments arranged by size: 2041 2007 1401 880 820 640 504 390 345 269 184 63 MscI TGG'CCA Cuts at: 10 64 1489 1832 2554 3478 4593 10 Size: 54 1425 343 722 924 1115 4961 Fragments arranged by size: 4961 1425 1115 924 722 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3219 5120 5622 5844 6158 7502 7861 Size: 648 2053 1901 502 222 314 1344 359 Cuts at: 7861 8020 518 Size: 159 2042 Fragments arranged by size: 2053 2042 1901 1344 648 502 359 314 222 159 MspA1I CmG'CkG Cuts at: 1606 2165 3718 6000 6960 7637 8785 9030 1606 Size: 559 1553 2282 960 677 1148 245 2120 Fragments arranged by size: 2282 2120 1553 1148 960 677 559 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2808 3369 513 Size: 552 939 804 561 6688 Fragments arranged by size: 6688 939 804 561 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9544 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9544 NotI GC'GGCC_GC Cuts at: 6991 6991 Size: 9544 NspI r_CATG'y Cuts at: 1132 1229 2310 2822 3385 4100 5751 6902 8447 Size: 97 1081 512 563 715 1651 1151 1545 Cuts at: 8447 1132 Size: 2229 Fragments arranged by size: 2229 1651 1545 1151 1081 715 563 512 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9544 PciI A'CATG_T Cuts at: 3381 4096 5747 8443 3381 Size: 715 1651 2696 4482 Fragments arranged by size: 4482 2696 1651 715 PflMI CCAn_nnn'nTGG Cuts at: 1108 3980 1108 Size: 2872 6672 PfoI T'CCnGG_A Cuts at: 2952 6313 2952 Size: 3361 6183 PmeI GTTT'AAAC Cuts at: 6848 6848 Size: 9544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5864 5896 7613 7645 9126 9158 5864 Size: 32 1717 32 1481 32 6250 Fragments arranged by size: 6250 1717 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5287 5287 Size: 9544 PshAI GACnn'nnGTC Cuts at: 1613 3081 1613 Size: 1468 8076 PsiI TTA'TAA Cuts at: 2531 7114 2531 Size: 4583 4961 PspXI vC'TCGA_Gb Cuts at: 5181 5181 Size: 9544 PstI C_TGCA'G Cuts at: 830 2039 3069 6602 6896 830 Size: 1209 1030 3533 294 3478 Fragments arranged by size: 3533 3478 1209 1030 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9544 PvuII CAG'CTG Cuts at: 2165 3718 2165 Size: 1553 7991 SacI G_AGCT'C Cuts at: 4261 5021 4261 Size: 760 8784 SalI G'TCGA_C Cuts at: 1936 6886 1936 Size: 4950 4594 SapI GCTCTTCn'nnn_ Cuts at: 4820 4820 Size: 9544 SbfI CC_TGCA'GG Cuts at: 6896 6896 Size: 9544 ScaI AGT'ACT Cuts at: 3582 7792 9504 3582 Size: 4210 1712 3622 Fragments arranged by size: 4210 3622 1712 SexAI A'CCwGG_T Cuts at: 5703 5703 Size: 9544 SfcI C'TryA_G Cuts at: 826 1045 2035 3065 3817 6553 6598 6892 8027 Size: 219 990 1030 752 2736 45 294 1135 Cuts at: 8027 8708 8899 826 Size: 681 191 1471 Fragments arranged by size: 2736 1471 1135 1030 990 752 681 294 219 191 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9544 SmaI CCC'GGG Cuts at: 1872 3350 6871 1872 Size: 1478 3521 4545 Fragments arranged by size: 4545 3521 1478 SmlI C'TyrA_G Cuts at: 820 1017 1943 3019 4052 5181 5305 6324 7648 Size: 197 926 1076 1033 1129 124 1019 1324 Cuts at: 7648 8549 8811 820 Size: 901 262 1553 Fragments arranged by size: 1553 1324 1129 1076 1033 1019 926 901 262 197 124 SnaBI TAC'GTA Cuts at: 493 7335 493 Size: 6842 2702 SpeI A'CTAG_T Cuts at: 152 3583 152 Size: 3431 6113 SphI G_CATG'C Cuts at: 1132 6902 1132 Size: 5770 3774 SspI AAT'ATT Cuts at: 5 52 7468 5 Size: 47 7416 2081 Fragments arranged by size: 7416 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9544 StyI C'CwwG_G Cuts at: 513 1065 2004 2808 3369 3492 6663 6983 513 Size: 552 939 804 561 123 3171 320 3074 Fragments arranged by size: 3171 3074 939 804 561 552 320 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 6303 6402 7571 7588 7741 7926 Size: 1255 2037 2686 99 1169 17 153 185 Cuts at: 7926 8371 325 Size: 445 1498 Fragments arranged by size: 2686 2037 1498 1255 1169 445 185 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2331 2460 2715 Size: 274 80 33 51 1443 354 129 255 Cuts at: 2715 3580 4161 6830 7790 9502 96 Size: 865 581 2669 960 1712 138 Fragments arranged by size: 2669 1712 1443 960 865 581 354 274 255 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4666 4698 4777 4809 5442 5474 9504 9536 4666 Size: 32 79 32 633 32 4030 32 4674 Fragments arranged by size: 4674 4030 633 79 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9544 XbaI T'CTAG_A Cuts at: 6880 6880 Size: 9544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4840 5891 4840 Size: 1051 8493 XhoI C'TCGA_G Cuts at: 1943 5181 6324 1943 Size: 3238 1143 5163 Fragments arranged by size: 5163 3238 1143 XmaI C'CCGG_G Cuts at: 1870 3348 6869 1870 Size: 1478 3521 4545 Fragments arranged by size: 4545 3521 1478 XmnI GAAnn'nnTTC Cuts at: 3675 7673 3675 Size: 3998 5546 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7352 276 Size: 53 83 186 936 5818 2468 Fragments arranged by size: 5818 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI