Restriction map for
pFN21AE3478
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BlnI BlpI
Bpu1102I BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssHII BssSI
BstAPI BstBI BstEII BstPI CelII
CfoI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 2259 CGCG CG^CG
AflII 3300 CTTAAG C^TTAAG
Alw44I 2081 GTGCAC G^TGCAC
ApaLI 2081 GTGCAC G^TGCAC
AseI 3112 ATTAAT AT^TAAT
AsnI 3112 ATTAAT AT^TAAT
Asp700I 1666 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 2798 CGACTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3300 CTTAAG C^TTAAG
BglI 1841 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4199 CACGTA [CT]AC^GT[GA]
BsaI 3236 GGTCTC GGTCTC(1/5)
BsaWI 3404 ACCGGA [AT]^CCGG[AT]
BsmI 123 GAATGC GAATGC(1/-1)
Bsp1407I 4825 TGTACA T^GTACA
BsrBI 53 GAGCGG CCGCTC(-3/-3)
BsrFI 4565 ACCGGC [GA]^CCGG[CT]
BsrGI 4825 TGTACA T^GTACA
BstUI 2259 CGCG CG^CG
Cfr10I 4565 ACCGGC [GA]^CCGG[CT]
DrdI 2632 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2101 GAATTC G^AATTC
EcoRV 1921 GATATC GAT^ATC
FauI 2373 GCGGG CCCGC(4/6)
HincII 1453 GTTAAC GT[CT]^[GA]AC
HindII 1453 GTTAAC GT[CT]^[GA]AC
HpaI 1453 GTTAAC GTT^AAC
MvnI 2259 CGCG CG^CG
PflMI 1969 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3281 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1072 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3112 ATTAAT AT^TAAT
SapI 2808 GCTCTTC GCTCTTC(1/4)
ScaI 1575 AGTACT AGT^ACT
SexAI 3698 ACCTGGT A^CC[AT]GGT
SmaI 1343 CCCGGG CCC^GGG
SpeI 1578 ACTAGT A^CTAGT
SspBI 4825 TGTACA T^GTACA
Van91I 1969 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1343 CCCGGG C^CCGGG
XmaI 1343 CCCGGG C^CCGGG
XmnI 1666 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AocI 12 CCTGAGG CC^T[ACGT]AGG
1819 CCTCAGG
BanI 976 GGTGCC G^G[CT][GA]CC
3907 GGTGCC
BciVI 1032 GGATAC GTATCC(6/5)
3788 GTATCC
BglII 1313 AGATCT A^GATCT
3363 AGATCT
BsgI 256 GTGCAG GTGCAG(16/14)
1685 GTGCAG
BsmBI 1017 GAGACG CGTCTC(1/5)
1569 GAGACG
BspMI 964 ACCTGC ACCTGC(4/8)
1876 ACCTGC
Bst1107I 756 GTATAC GTA^TAC
4168 GTATAC
BstXI 1209 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3692 CCATGCACCTGG
BstZ17I 756 GTATAC GTA^TAC
4168 GTATAC
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
1819 CCTCAGG
DraII 1823 AGGGCCT [GA]G^G[ACGT]CC[CT]
3281 AGGTCCT
DsaI 803 CCATGG C^C[GA][CT]GG
1364 CCATGG
EarI 2809 CTCTTC CTCTTC(1/4)
4304 CTCTTC
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
1819 CCTCAGG
EcoNI 1105 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2269 CCTGCCGAAGG
EcoO109I 1823 AGGGCCT [GA]G^G[ACGT]CC[CT]
3281 AGGTCCT
HgaI 1019 GACGC GACGC(5/10)
2555 GACGC
HindIII 583 AAGCTT A^AGCTT
1988 AAGCTT
Ksp632I 2809 CTCTTC CTCTTC(1/4)
4304 CTCTTC
MfeI 3311 CAATTG C^AATTG
3849 CAATTG
MunI 3311 CAATTG C^AATTG
3849 CAATTG
NcoI 803 CCATGG C^CATGG
1364 CCATGG
PaeR7I 3176 CTCGAG C^TCGAG
4319 CTCGAG
PvuII 158 CAGCTG CAG^CTG
1711 CAGCTG
SacI 2252 GAGCTC GAGCT^C
3012 GAGCTC
XcmI 2828 CCATCAACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3879 CCAGTGTGCCAGTGG
XhoI 3176 CTCGAG C^TCGAG
4319 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII