Restriction map for
pFN21AE3478
(Linear) (Six-base) MAPSORT of: KIEE3478.seq Check: 4297 from: 1 to: 4839
KIEE3478.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 757 2135 4169 4839
Size: 757 1378 2034 670
Fragments arranged by size:
2034 1378 757 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 2796 3211 4109 4839
Size: 804 1992 415 898 730
Fragments arranged by size:
1992 898 804 730 415
AflII C'TTAA_G
Cuts at: 0 3300 4839
Size: 3300 1539
AflIII A'CryG_T
Cuts at: 0 1376 2091 3742 4839
Size: 1376 715 1651 1097
Fragments arranged by size:
1651 1376 1097 715
AleI CACnn'nnGTG
Cuts at: 0 3617 4839
Size: 3617 1222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 857 3635 4839
Size: 857 2778 1204
Fragments arranged by size:
2778 1204 857
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3266 3298 4839
Size: 3266 32 1541
Fragments arranged by size:
3266 1541 32
AlwNI CAG_nnn'CTG
Cuts at: 0 38 340 1264 3033 4258 4696 4839
Size: 38 302 924 1769 1225 438 143
Fragments arranged by size:
1769 1225 924 438 302 143 38
ApaLI G'TGCA_C
Cuts at: 0 2081 4839
Size: 2081 2758
ApoI r'AATT_y
Cuts at: 0 235 539 1400 1592 1931 2024 2101 4501
Size: 235 304 861 192 339 93 77 2400
Cuts at: 4501 4839
Size: 338
Fragments arranged by size:
2400 861 339 338 304 235 192 93
77
AseI AT'TA_AT
Cuts at: 0 3113 4839
Size: 3113 1726
AvaI C'yCGr_G
Cuts at: 0 1343 3176 4319 4839
Size: 1343 1833 1143 520
Fragments arranged by size:
1833 1343 1143 520
BanI G'GyrC_C
Cuts at: 0 976 3907 4839
Size: 976 2931 932
Fragments arranged by size:
2931 976 932
BanII G_rGCy'C
Cuts at: 0 2256 2468 2863 3016 4389 4839
Size: 2256 212 395 153 1373 450
Fragments arranged by size:
2256 1373 450 395 212 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 1076 1592 1879 3425 3836 4839
Size: 1076 516 287 1546 411 1003
Fragments arranged by size:
1546 1076 1003 516 411 287
BbvCI CC'TCA_GC
Cuts at: 0 1778 3055 3752 4839
Size: 1778 1277 697 1087
Fragments arranged by size:
1778 1277 1087 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2787 2821 4839
Size: 2787 34 2018
Fragments arranged by size:
2787 2018 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1026 3799 4839
Size: 1026 2773 1040
Fragments arranged by size:
2773 1040 1026
BclI T'GATC_A
Cuts at: 0 1700 3984 4035 4334 4839
Size: 1700 2284 51 299 505
Fragments arranged by size:
2284 1700 505 299 51
BglI GCCn_nnn'nGGC
Cuts at: 0 1847 4839
Size: 1847 2992
BglII A'GATC_T
Cuts at: 0 1313 3363 4839
Size: 1313 2050 1476
Fragments arranged by size:
2050 1476 1313
Bme1580I G_kGCm'C
Cuts at: 0 2085 2696 3912 4703 4839
Size: 2085 611 1216 791 136
Fragments arranged by size:
2085 1216 791 611 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 944 1242 1401 1524 1574 2169 3867
Size: 477 467 298 159 123 50 595 1698
Cuts at: 3867 4839
Size: 972
Fragments arranged by size:
1698 972 595 477 467 298 159 123
50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4808 4839
Size: 4808 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 744 884 970 2568 4839
Size: 219 525 140 86 1598 2271
Fragments arranged by size:
2271 1598 525 219 140 86
Bpu10I CC'TnA_GC
Cuts at: 0 1778 3055 3623 3752 4839
Size: 1778 1277 568 129 1087
Fragments arranged by size:
1778 1277 1087 568 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 2032 4839
Size: 1035 997 2807
Fragments arranged by size:
2807 1035 997
BsaI GGTCTCn'nnnn_
Cuts at: 0 3242 4839
Size: 3242 1597
BsaAI yAC'GTr
Cuts at: 0 4201 4839
Size: 4201 638
BsaBI GATnn'nnATC
Cuts at: 0 1100 2632 3362 4079 4839
Size: 1100 1532 730 717 760
Fragments arranged by size:
1532 1100 760 730 717
BsaWI w'CCGG_w
Cuts at: 0 3404 4839
Size: 3404 1435
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3732 3762 4839
Size: 3732 30 1077
Fragments arranged by size:
3732 1077 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 3740 4756 4797 4824 4839
Size: 30 3710 1016 41 27 15
Fragments arranged by size:
3710 1016 41 30 27 15
BseYI C'CCAG_C
Cuts at: 0 3336 3557 4470 4839
Size: 3336 221 913 369
Fragments arranged by size:
3336 913 369 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1706 4839
Size: 277 1429 3133
Fragments arranged by size:
3133 1429 277
BsiHKAI G_wGCw'C
Cuts at: 0 47 730 2085 2256 2793 3016 3624 4839
Size: 47 683 1355 171 537 223 608 1215
Fragments arranged by size:
1355 1215 683 608 537 223 171 47
BsmI GAATG_Cn'
Cuts at: 0 129 4839
Size: 129 4710
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1011 1563 4839
Size: 1011 552 3276
Fragments arranged by size:
3276 1011 552
Bsp1286I G_dGCh'C
Cuts at: 0 47 730 2085 2256 2468 2696 2793 2863
Size: 47 683 1355 171 212 228 97 70
Cuts at: 2863 3016 3624 3912 4389 4703 4839
Size: 153 608 288 477 314 136
Fragments arranged by size:
1355 683 608 477 314 288 228 212
171 153 136 97 70 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 973 1885 4839
Size: 973 912 2954
Fragments arranged by size:
2954 973 912
BsrBI CCG'CTC
Cuts at: 0 55 4839
Size: 55 4784
BsrDI GCAATG_nn'
Cuts at: 0 1085 2336 2694 3927 4839
Size: 1085 1251 358 1233 912
Fragments arranged by size:
1251 1233 1085 912 358
BsrFI r'CCGG_y
Cuts at: 0 4565 4839
Size: 4565 274
BsrGI T'GTAC_A
Cuts at: 0 4825 4839
Size: 4825 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 3699 4839
Size: 1216 2483 1140
Fragments arranged by size:
2483 1216 1140
BstYI r'GATC_y
Cuts at: 0 376 435 1171 1313 1359 3363 4300 4839
Size: 376 59 736 142 46 2004 937 539
Fragments arranged by size:
2004 937 736 539 376 142 59 46
BstZ17I GTA'TAC
Cuts at: 0 758 4170 4839
Size: 758 3412 669
Fragments arranged by size:
3412 758 669
Bsu36I CC'TnA_GG
Cuts at: 0 13 1820 4839
Size: 13 1807 3019
Fragments arranged by size:
3019 1807 13
BtgI C'CryG_G
Cuts at: 0 803 1364 4839
Size: 803 561 3475
Fragments arranged by size:
3475 803 561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3824 4839
Size: 3824 1015
BtsI GCAGTG_nn'
Cuts at: 0 659 1193 2085 2694 3390 3901 4059 4371
Size: 659 534 892 609 696 511 158 312
Cuts at: 4371 4839
Size: 468
Fragments arranged by size:
892 696 659 609 534 511 468 312
158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2565 2600 4839
Size: 2565 35 2239
Fragments arranged by size:
2565 2239 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2638 4839
Size: 2638 2201
EaeI y'GGCC_r
Cuts at: 0 547 1471 2586 4839
Size: 547 924 1115 2253
Fragments arranged by size:
2253 1115 924 547
EarI CTCTTCn'nnn_
Cuts at: 0 2815 4310 4839
Size: 2815 1495 529
Fragments arranged by size:
2815 1495 529
EcoICRI GAG'CTC
Cuts at: 0 2254 3014 4839
Size: 2254 760 1825
Fragments arranged by size:
2254 1825 760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 744 804 884 970 2568 2796 3211
Size: 219 525 60 80 86 1598 228 415
Cuts at: 3211 4109 4839
Size: 898 730
Fragments arranged by size:
1598 898 730 525 415 228 219 86
80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1109 2273 4839
Size: 1109 1164 2566
Fragments arranged by size:
2566 1164 1109
EcoO109I rG'GnC_Cy
Cuts at: 0 1824 3282 4839
Size: 1824 1458 1557
Fragments arranged by size:
1824 1557 1458
EcoRI G'AATT_C
Cuts at: 0 2101 4839
Size: 2101 2738
EcoRV GAT'ATC
Cuts at: 0 1923 4839
Size: 1923 2916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 685 3701 4433 4839
Size: 685 3016 732 406
Fragments arranged by size:
3016 732 685 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1692 1724 1907 1939 3121 3153
Size: 781 32 879 32 183 32 1182 32
Cuts at: 3153 3706 3738 4179 4211 4839
Size: 553 32 441 32 628
Fragments arranged by size:
1182 879 781 628 553 441 183 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1455 4839
Size: 1455 3384
HindIII A'AGCT_T
Cuts at: 0 583 1988 4839
Size: 583 1405 2851
Fragments arranged by size:
2851 1405 583
HpaI GTT'AAC
Cuts at: 0 1455 4839
Size: 1455 3384
MfeI C'AATT_G
Cuts at: 0 3311 3849 4839
Size: 3311 538 990
Fragments arranged by size:
3311 990 538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 389 1790 2180 2243 3123 3763 4108
Size: 120 269 1401 390 63 880 640 345
Cuts at: 4108 4612 4839
Size: 504 227
Fragments arranged by size:
1401 880 640 504 390 345 269 227
120 63
MscI TGG'CCA
Cuts at: 0 549 1473 2588 4839
Size: 549 924 1115 2251
Fragments arranged by size:
2251 1115 924 549
MslI CAynn'nnrTG
Cuts at: 0 1214 3115 3617 3839 4153 4839
Size: 1214 1901 502 222 314 686
Fragments arranged by size:
1901 1214 686 502 314 222
MspA1I CmG'CkG
Cuts at: 0 160 1713 3995 4839
Size: 160 1553 2282 844
Fragments arranged by size:
2282 1553 844 160
NcoI C'CATG_G
Cuts at: 0 803 1364 4839
Size: 803 561 3475
Fragments arranged by size:
3475 803 561
NspI r_CATG'y
Cuts at: 0 305 817 1380 2095 3746 4839
Size: 305 512 563 715 1651 1093
Fragments arranged by size:
1651 1093 715 563 512 305
PciI A'CATG_T
Cuts at: 0 1376 2091 3742 4839
Size: 1376 715 1651 1097
Fragments arranged by size:
1651 1376 1097 715
PflMI CCAn_nnn'nTGG
Cuts at: 0 1975 4839
Size: 1975 2864
PfoI T'CCnGG_A
Cuts at: 0 947 4308 4839
Size: 947 3361 531
Fragments arranged by size:
3361 947 531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3859 3891 4839
Size: 3859 32 948
Fragments arranged by size:
3859 948 32
PpuMI rG'GwC_Cy
Cuts at: 0 3282 4839
Size: 3282 1557
PshAI GACnn'nnGTC
Cuts at: 0 1076 4839
Size: 1076 3763
PsiI TTA'TAA
Cuts at: 0 526 4839
Size: 526 4313
PspXI vC'TCGA_Gb
Cuts at: 0 3176 4839
Size: 3176 1663
PstI C_TGCA'G
Cuts at: 0 34 1064 4597 4839
Size: 34 1030 3533 242
Fragments arranged by size:
3533 1030 242 34
PvuII CAG'CTG
Cuts at: 0 160 1713 4839
Size: 160 1553 3126
Fragments arranged by size:
3126 1553 160
SacI G_AGCT'C
Cuts at: 0 2256 3016 4839
Size: 2256 760 1823
Fragments arranged by size:
2256 1823 760
SapI GCTCTTCn'nnn_
Cuts at: 0 2815 4839
Size: 2815 2024
ScaI AGT'ACT
Cuts at: 0 1577 4839
Size: 1577 3262
SexAI A'CCwGG_T
Cuts at: 0 3698 4839
Size: 3698 1141
SfcI C'TryA_G
Cuts at: 0 30 1060 1812 4548 4593 4839
Size: 30 1030 752 2736 45 246
Fragments arranged by size:
2736 1030 752 246 45 30
SmaI CCC'GGG
Cuts at: 0 1345 4839
Size: 1345 3494
SmlI C'TyrA_G
Cuts at: 0 1014 2047 3176 3300 4319 4839
Size: 1014 1033 1129 124 1019 520
Fragments arranged by size:
1129 1033 1019 1014 520 124
SpeI A'CTAG_T
Cuts at: 0 1578 4839
Size: 1578 3261
StyI C'CwwG_G
Cuts at: 0 803 1364 1487 4658 4839
Size: 803 561 123 3171 181
Fragments arranged by size:
3171 803 561 181 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1612 4298 4397 4839
Size: 1612 2686 99 442
Fragments arranged by size:
2686 1612 442 99
TatI w'GTAC_w
Cuts at: 0 326 455 710 1575 2156 4825 4839
Size: 326 129 255 865 581 2669 14
Fragments arranged by size:
2669 865 581 326 255 129 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2661 2693 2772 2804 3437 3469 4839
Size: 2661 32 79 32 633 32 1370
Fragments arranged by size:
2661 1370 633 79 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2835 3886 4839
Size: 2835 1051 953
Fragments arranged by size:
2835 1051 953
XhoI C'TCGA_G
Cuts at: 0 3176 4319 4839
Size: 3176 1143 520
Fragments arranged by size:
3176 1143 520
XmaI C'CCGG_G
Cuts at: 0 1343 4839
Size: 1343 3496
XmnI GAAnn'nnTTC
Cuts at: 0 1670 4839
Size: 1670 3169
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI
NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI
PspXI PstI PvuII SacI SapI ScaI SexAI SfcI
SmaI SmlI SpeI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI ApaI
AscI AsiSI AvrII BaeI BamHI BbeI BfrBI BlpI
BmgBI BmtI BsaHI BsiEI BsiWI BspEI BspHI BssHII
BssSI BstAPI BstBI BstEII ClaI DraI DraIII EagI
EciI FseI FspI FspAI HaeII KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SspI StuI SwaI Tth111I XbaI
ZraI