PDLIM4 ORF clone FHC11262

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Halotag ORF Clone FHC11262

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Clone info.

Species

human

Clone Name

pFN21AE0757 Sequence (with vector) Restriction Map

Product ID

FHC11262

Flexi Clone PID

FXC11262

ORF Sequence

DNA sequence (990 bp)
Protein sequence (330 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528934]

Gene

KIEE0757

Symbol

PDLIM4

Alias : RIL

Description

PDZ and LIM domain 4, transcript variant 1

Original Clone ID

cs02257

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 990 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 330 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4152.1 1.4e-75 99.7 PDLIM4 CCDS47261.1 8.8e-50 95.0 PDLIM4 CCDS7441.1 1.7e-26 44.6 PDLIM1 CCDS75218.1 4.5e-14 43.6 PDLIM3 CCDS75219.1 5.8e-14 45.0 PDLIM3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001478 9 80 PF00595 PDZ/DHR/GLGF IPR001781 255 305 PF00412 Zinc finger HMMSmart IPR001478 11 84 SM00228 PDZ/DHR/GLGF IPR001781 254 305 SM00132 Zinc finger ProfileScan IPR001478 8 84 PS50106 PDZ/DHR/GLGF IPR001781 253 312 PS50023 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003678 330 166710,603422 99.7 100.0 XP_016865491 252 166710,603422 76.1 100.0 NP_066272 329 605900 44.6 97.3 NP_001244891 276 605889 45.0 98.5 NP_001107579 316 605889 44.3 98.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0757 Download

>KIEE0757  990 bp
ATGCCCCATTCCGTGACCCTGCGCGGGCCTTCGCCCTGGGGCTTCCGCCTGGTGGGCGGC
CGGGACTTCAGCGCGCCCCTCACCATCTCACGGGTCCATGCTGGCAGCAAGGCTGCATTG
GCTGCCCTGTGCCCAGGAGACCTGATCCAGGCCATCAATGGTGAGAGCACAGAGCTCATG
ACACACCTGGAGGCACAGAACCGCATCAAGGGCTGCCACGATCACCTCACACTGTCTGTG
AGCAGGCCTGAGGGCAGGAGCTGGCCCAGTGCCCCTGATGACAGCAAGGCTCAGGCACAC
AGGATCCACATCGATCCTGAGATCCAGGACGGCAGCCCAACAACCAGCAGGCGGCCCTCA
GGCACCGGGACTGGGCCAGAAGATGGCAGACCAAGCCTGGGATCTCCATATGGACAACCC
CCTCGCTTTCCAGTCCCTCACAATGGCAGCAGCGAGGCCACCCTGCCAGCCCAGATGAGC
ACCCTGCATGTGTCTCCACCCCCCAGCGCTGACCCAGCCAGAGGCCTCCCGCGGAGCCGG
GACTGCAGAGTCGACCTGGGCTCCGAGGTGTACAGGATGCTGCGGGAGCCAGCCGAGCCC
GTGGCCGCGGAGCCCAAGCAGTCAGGCTCCTTCCGCTACTTGCAGGGCATGCTAGAGGCC
GGCGAGGGCGGGGATTGGCCCGGGCCTGGCGGCCCCCGGAACCTCAAGCCCACGGCCAGC
AAGCTGGGCGCTCCGCTGAGCGGCCTGCAGGGGCTGCCCGAGTGCACGCGCTGCTGCCAC
GGCATCGTGGGCACCATCGTCAAGGCACGGGACAAGCTCTACCATCCCGAGTGCTTCATG
TGCAGTGACTGCGGCCTGAACCTCAAGCAGCGTGGTTACTTCTTTCTGGACGAGCGGCTC
TACTGTGAGAGCCACGCCAAGGCGCGCGTGAAGCCGCCCGAGGGCTACGACGTGGTGGCG
GTGTACCCCAATGCCAAGGTGGAACTCGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE0757 Download

>KIEE0757  330 aa
MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELM
THLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRRPS
GTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSR
DCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTAS
KLGAPLSGLQGLPECTRCCHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERL
YCESHAKARVKPPEGYDVVAVYPNAKVELV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0757 Download

>pFN21AE0757  5695 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCCATTCCGTGACCCTGCGCGGGCCTTCGCC
CTGGGGCTTCCGCCTGGTGGGCGGCCGGGACTTCAGCGCGCCCCTCACCATCTCACGGGT
CCATGCTGGCAGCAAGGCTGCATTGGCTGCCCTGTGCCCAGGAGACCTGATCCAGGCCAT
CAATGGTGAGAGCACAGAGCTCATGACACACCTGGAGGCACAGAACCGCATCAAGGGCTG
CCACGATCACCTCACACTGTCTGTGAGCAGGCCTGAGGGCAGGAGCTGGCCCAGTGCCCC
TGATGACAGCAAGGCTCAGGCACACAGGATCCACATCGATCCTGAGATCCAGGACGGCAG
CCCAACAACCAGCAGGCGGCCCTCAGGCACCGGGACTGGGCCAGAAGATGGCAGACCAAG
CCTGGGATCTCCATATGGACAACCCCCTCGCTTTCCAGTCCCTCACAATGGCAGCAGCGA
GGCCACCCTGCCAGCCCAGATGAGCACCCTGCATGTGTCTCCACCCCCCAGCGCTGACCC
AGCCAGAGGCCTCCCGCGGAGCCGGGACTGCAGAGTCGACCTGGGCTCCGAGGTGTACAG
GATGCTGCGGGAGCCAGCCGAGCCCGTGGCCGCGGAGCCCAAGCAGTCAGGCTCCTTCCG
CTACTTGCAGGGCATGCTAGAGGCCGGCGAGGGCGGGGATTGGCCCGGGCCTGGCGGCCC
CCGGAACCTCAAGCCCACGGCCAGCAAGCTGGGCGCTCCGCTGAGCGGCCTGCAGGGGCT
GCCCGAGTGCACGCGCTGCTGCCACGGCATCGTGGGCACCATCGTCAAGGCACGGGACAA
GCTCTACCATCCCGAGTGCTTCATGTGCAGTGACTGCGGCCTGAACCTCAAGCAGCGTGG
TTACTTCTTTCTGGACGAGCGGCTCTACTGTGAGAGCCACGCCAAGGCGCGCGTGAAGCC
GCCCGAGGGCTACGACGTGGTGGCGGTGTACCCCAATGCCAAGGTGGAACTCGTCGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT