(Circular) (Six-base) MAPSORT of: pFN21AE0757.seq Check: 9081 from: 1 to: 5695 pFN21AE0757 5695 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 3038 1937 Size: 619 482 4594 Fragments arranged by size: 4594 619 482 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2056 3758 5142 5410 1632 Size: 424 1702 1384 268 1917 Fragments arranged by size: 1917 1702 1384 424 268 AfeI AGC'GCT Cuts at: 2512 2512 Size: 5695 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2265 2758 2853 3438 5010 2265 Size: 493 95 585 1572 2950 Fragments arranged by size: 2950 1572 585 493 95 ApaLI G'TGCA_C Cuts at: 2767 3752 4908 2767 Size: 985 1156 3554 Fragments arranged by size: 3554 1156 985 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2684 2762 2831 2942 3020 1870 Size: 73 741 78 69 111 78 4545 Fragments arranged by size: 4545 741 111 78 78 73 69 BamHI G'GATC_C Cuts at: 2307 3025 3392 2307 Size: 718 367 4610 Fragments arranged by size: 4610 718 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2795 3016 4471 618 Size: 325 217 627 579 429 221 1455 1842 Fragments arranged by size: 1842 1455 627 579 429 325 221 217 BanII G_rGCy'C Cuts at: 1391 2181 2567 2604 2619 3014 1391 Size: 790 386 37 15 395 4072 Fragments arranged by size: 4072 790 395 386 37 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3886 3920 1903 Size: 34 50 34 1865 34 3678 Fragments arranged by size: 3678 1865 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 4306 136 Size: 107 122 71 631 605 386 2248 1525 Fragments arranged by size: 2248 1525 631 605 386 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 2741 3112 2741 Size: 371 5324 Bme1580I G_kGCm'C Cuts at: 1790 2138 2278 2771 2798 3756 4912 1790 Size: 348 140 493 27 958 1156 2573 Fragments arranged by size: 2573 1156 958 493 348 140 27 BmgBI CAC'GTC Cuts at: 1318 2956 1318 Size: 1638 4057 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2265 2385 3426 Size: 416 53 83 399 24 848 120 1041 Cuts at: 3426 4384 442 Size: 958 1753 Fragments arranged by size: 1753 1041 958 848 416 399 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 4355 1239 Size: 974 2142 2579 Fragments arranged by size: 2579 2142 974 Bpu10I CC'TnA_GC Cuts at: 2295 2295 Size: 5695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 2852 3820 4685 4983 2693 Size: 159 968 865 298 3405 Fragments arranged by size: 3405 968 865 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2136 4358 882 Size: 617 637 2222 2219 Fragments arranged by size: 2222 2219 637 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 2312 5689 2312 Size: 3377 2318 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 4294 Fragments arranged by size: 4294 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 2507 2518 2728 4898 5394 2507 Size: 11 210 2170 496 2808 Fragments arranged by size: 2808 2170 496 210 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2866 1163 Size: 573 1130 3992 Fragments arranged by size: 3992 1130 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2065 3145 3906 4934 1540 Size: 275 186 64 1080 761 1028 2301 Fragments arranged by size: 2301 1080 1028 761 275 186 64 BsiHKAI G_wGCw'C Cuts at: 2174 2181 2486 2771 3756 3841 4912 2174 Size: 7 305 285 985 85 1071 2957 Fragments arranged by size: 2957 1071 985 305 285 85 7 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2174 2181 2278 2486 2567 2604 Size: 399 348 36 7 97 208 81 37 Cuts at: 2604 2619 2771 2798 3014 3756 3841 4912 1391 Size: 15 152 27 216 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 216 208 152 97 85 81 37 36 27 15 7 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 2181 3584 5314 2181 Size: 1403 1730 2562 Fragments arranged by size: 2562 1730 1403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3050 844 Size: 2206 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2745 2899 3582 1394 Size: 429 922 154 683 3507 Fragments arranged by size: 3507 922 683 429 154 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2663 4339 4526 1700 Size: 110 853 1676 187 2869 Fragments arranged by size: 2869 1676 853 187 110 BsrGI T'GTAC_A Cuts at: 96 2574 96 Size: 2478 3217 BssHII G'CGCG_C Cuts at: 1925 2076 2927 1925 Size: 151 851 4693 Fragments arranged by size: 4693 851 151 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 2126 2653 2758 2775 2853 794 Size: 1332 527 105 17 78 3636 Fragments arranged by size: 3636 1332 527 105 78 17 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2307 2325 2405 3025 3392 3777 3794 5246 Size: 335 18 80 620 367 385 17 1452 Cuts at: 5246 5332 5344 5690 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 620 385 367 346 335 86 80 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2253 2362 2253 Size: 109 5586 BtgI C'CryG_G Cuts at: 513 1065 2534 2604 2610 2715 2782 513 Size: 552 1469 70 6 105 67 3426 Fragments arranged by size: 3426 1469 552 105 70 67 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2854 3211 4005 4025 2854 Size: 357 794 20 4524 Fragments arranged by size: 4524 794 357 20 ClaI AT'CG_AT Cuts at: 1554 2316 1554 Size: 762 4933 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2857 2892 529 Size: 35 2293 35 3332 Fragments arranged by size: 3332 2293 35 35 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2552 4702 809 Size: 507 1236 2150 1802 Fragments arranged by size: 2150 1802 1236 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2607 2718 Size: 54 1425 50 275 18 232 545 111 Cuts at: 2718 3142 4031 5660 8 Size: 424 889 1629 43 Fragments arranged by size: 1629 1425 889 545 424 275 232 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2062 3142 1537 Size: 275 250 1080 4090 Fragments arranged by size: 4090 1080 275 250 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 4282 4656 4802 387 Size: 1064 138 450 2243 374 146 1280 Fragments arranged by size: 2243 1280 1064 450 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 2179 Size: 5695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2213 3758 4355 5142 5410 1239 Size: 393 424 157 1545 597 787 268 1524 Fragments arranged by size: 1545 1524 787 597 424 393 268 157 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2150 Size: 5695 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2514 2736 4842 1678 Size: 836 222 2106 2531 Fragments arranged by size: 2531 2106 836 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2549 2581 4336 Size: 32 135 32 561 32 384 32 1755 Cuts at: 4336 4368 4410 4442 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1755 561 384 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2557 3039 3285 669 Size: 988 281 619 482 246 3079 Fragments arranged by size: 3079 988 619 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3653 4012 4171 518 Size: 648 2487 359 159 2042 Fragments arranged by size: 2487 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2536 2612 2740 3111 3788 4936 5181 1606 Size: 930 76 128 371 677 1148 245 2120 Fragments arranged by size: 2120 1148 930 677 371 245 128 76 NaeI GCC'GGC Cuts at: 2665 2665 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5143 NdeI CA'TA_TG Cuts at: 387 2413 387 Size: 2026 3669 NgoMIV G'CCGG_C Cuts at: 2663 2663 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2495 2656 3053 4598 1132 Size: 97 1266 161 397 1545 2229 Fragments arranged by size: 2229 1545 1266 397 161 97 PasI CC'CwG_GG Cuts at: 1348 2040 1348 Size: 692 5003 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2387 1108 Size: 1279 4416 PfoI T'CCnGG_A Cuts at: 2328 2328 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PstI C_TGCA'G Cuts at: 830 2552 2754 3047 830 Size: 1722 202 293 3478 Fragments arranged by size: 3478 1722 293 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SacI G_AGCT'C Cuts at: 2181 2181 Size: 5695 SacII CC_GC'GG Cuts at: 2537 2613 2537 Size: 76 5619 SalI G'TCGA_C Cuts at: 1936 2555 3037 1936 Size: 619 482 4594 Fragments arranged by size: 4594 619 482 SbfI CC_TGCA'GG Cuts at: 2754 3047 2754 Size: 293 5402 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2548 2750 3043 4178 4859 5050 826 Size: 219 1503 202 293 1135 681 191 1471 Fragments arranged by size: 1503 1471 1135 681 293 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2686 3022 1872 Size: 814 336 4545 Fragments arranged by size: 4545 814 336 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 2867 3799 4700 4962 820 Size: 197 926 765 159 932 901 262 1553 Fragments arranged by size: 1553 932 926 901 765 262 197 159 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 2656 3053 1132 Size: 1524 397 3774 Fragments arranged by size: 3774 1524 397 SrfI GCCC'GGGC Cuts at: 2686 2686 Size: 5695 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2251 2529 1517 Size: 734 278 4683 Fragments arranged by size: 4683 734 278 StyI C'CwwG_G Cuts at: 513 1065 2922 2979 3134 513 Size: 552 1857 57 155 3074 Fragments arranged by size: 3074 1857 552 155 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3941 5653 Size: 274 80 33 51 1443 597 1367 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1443 1367 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2292 2324 5655 5687 2292 Size: 32 3331 32 2300 Fragments arranged by size: 3331 2300 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2159 2980 2159 Size: 821 4874 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2684 3020 1870 Size: 814 336 4545 Fragments arranged by size: 4545 814 336 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SwaI