Restriction map for
pFN21AE0757
(Linear) (Six-base) MAPSORT of: KIEE0757.seq Check: 5562 from: 1 to: 990
KIEE0757.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 551 990
Size: 551 439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 990
Size: 51 939
AfeI AGC'GCT
Cuts at: 0 507 990
Size: 507 483
AlwNI CAG_nnn'CTG
Cuts at: 0 260 753 848 990
Size: 260 493 95 142
Fragments arranged by size:
493 260 142 95
ApaLI G'TGCA_C
Cuts at: 0 762 990
Size: 762 228
AvaI C'yCGr_G
Cuts at: 0 679 757 826 937 990
Size: 679 78 69 111 53
Fragments arranged by size:
679 111 78 69 53
BamHI G'GATC_C
Cuts at: 0 302 990
Size: 302 688
BanI G'GyrC_C
Cuts at: 0 361 790 990
Size: 361 429 200
Fragments arranged by size:
429 361 200
BanII G_rGCy'C
Cuts at: 0 176 562 599 614 990
Size: 176 386 37 15 376
Fragments arranged by size:
386 376 176 37 15
BglI GCCn_nnn'nGGC
Cuts at: 0 53 990
Size: 53 937
BlpI GC'TnA_GC
Cuts at: 0 736 990
Size: 736 254
Bme1580I G_kGCm'C
Cuts at: 0 133 273 766 793 990
Size: 133 140 493 27 197
Fragments arranged by size:
493 197 140 133 27
BmgBI CAC'GTC
Cuts at: 0 951 990
Size: 951 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 380 990
Size: 260 120 610
Fragments arranged by size:
610 260 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 990
Size: 208 782
Bpu10I CC'TnA_GC
Cuts at: 0 290 990
Size: 290 700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 847 990
Size: 688 159 143
Fragments arranged by size:
688 159 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 131 990
Size: 131 859
BsaBI GATnn'nnATC
Cuts at: 0 307 990
Size: 307 683
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 990
Size: 544 30 416
Fragments arranged by size:
544 416 30
BseYI C'CCAG_C
Cuts at: 0 502 513 723 990
Size: 502 11 210 267
Fragments arranged by size:
502 267 210 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 990
Size: 861 129
BsiEI CG_ry'CG
Cuts at: 0 60 990
Size: 60 930
BsiHKAI G_wGCw'C
Cuts at: 0 169 176 481 766 990
Size: 169 7 305 285 224
Fragments arranged by size:
305 285 224 169 7
Bsp1286I G_dGCh'C
Cuts at: 0 133 169 176 273 481 562 599 614
Size: 133 36 7 97 208 81 37 15
Cuts at: 614 766 793 990
Size: 152 27 197
Fragments arranged by size:
208 197 152 133 97 81 37 36
27 15 7
BspHI T'CATG_A
Cuts at: 0 176 990
Size: 176 814
BsrBI CCG'CTC
Cuts at: 0 740 894 990
Size: 740 154 96
Fragments arranged by size:
740 154 96
BsrFI r'CCGG_y
Cuts at: 0 658 990
Size: 658 332
BsrGI T'GTAC_A
Cuts at: 0 569 990
Size: 569 421
BssHII G'CGCG_C
Cuts at: 0 71 922 990
Size: 71 851 68
Fragments arranged by size:
851 71 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 121 648 753 770 848 990
Size: 121 527 105 17 78 142
Fragments arranged by size:
527 142 121 105 78 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 990
Size: 723 267
BstYI r'GATC_y
Cuts at: 0 302 320 400 990
Size: 302 18 80 590
Fragments arranged by size:
590 302 80 18
Bsu36I CC'TnA_GG
Cuts at: 0 248 357 990
Size: 248 109 633
Fragments arranged by size:
633 248 109
BtgI C'CryG_G
Cuts at: 0 529 599 605 710 777 990
Size: 529 70 6 105 67 213
Fragments arranged by size:
529 213 105 70 67 6
BtsI GCAGTG_nn'
Cuts at: 0 849 990
Size: 849 141
ClaI AT'CG_AT
Cuts at: 0 311 990
Size: 311 679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 852 887 990
Size: 852 35 103
Fragments arranged by size:
852 103 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 990
Size: 547 443
EaeI y'GGCC_r
Cuts at: 0 57 602 713 990
Size: 57 545 111 277
Fragments arranged by size:
545 277 111 57
EagI C'GGCC_G
Cuts at: 0 57 990
Size: 57 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 990
Size: 34 956
EcoICRI GAG'CTC
Cuts at: 0 174 990
Size: 174 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 208 990
Size: 51 157 782
Fragments arranged by size:
782 157 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 990
Size: 145 845
HaeII r_GCGC'y
Cuts at: 0 509 731 990
Size: 509 222 259
Fragments arranged by size:
509 259 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 544 576 990
Size: 128 32 384 32 414
Fragments arranged by size:
414 384 128 32 32
HincII GTy'rAC
Cuts at: 0 552 990
Size: 552 438
MspA1I CmG'CkG
Cuts at: 0 531 607 735 990
Size: 531 76 128 255
Fragments arranged by size:
531 255 128 76
NaeI GCC'GGC
Cuts at: 0 660 990
Size: 660 330
NdeI CA'TA_TG
Cuts at: 0 408 990
Size: 408 582
NgoMIV G'CCGG_C
Cuts at: 0 658 990
Size: 658 332
NspI r_CATG'y
Cuts at: 0 490 651 990
Size: 490 161 339
Fragments arranged by size:
490 339 161
PasI CC'CwG_GG
Cuts at: 0 35 990
Size: 35 955
PflMI CCAn_nnn'nTGG
Cuts at: 0 382 990
Size: 382 608
PfoI T'CCnGG_A
Cuts at: 0 323 990
Size: 323 667
PstI C_TGCA'G
Cuts at: 0 547 749 990
Size: 547 202 241
Fragments arranged by size:
547 241 202
SacI G_AGCT'C
Cuts at: 0 176 990
Size: 176 814
SacII CC_GC'GG
Cuts at: 0 532 608 990
Size: 532 76 382
Fragments arranged by size:
532 382 76
SalI G'TCGA_C
Cuts at: 0 550 990
Size: 550 440
SbfI CC_TGCA'GG
Cuts at: 0 749 990
Size: 749 241
SfcI C'TryA_G
Cuts at: 0 543 745 990
Size: 543 202 245
Fragments arranged by size:
543 245 202
SmaI CCC'GGG
Cuts at: 0 681 990
Size: 681 309
SmlI C'TyrA_G
Cuts at: 0 703 862 990
Size: 703 159 128
Fragments arranged by size:
703 159 128
SphI G_CATG'C
Cuts at: 0 651 990
Size: 651 339
SrfI GCCC'GGGC
Cuts at: 0 681 990
Size: 681 309
StuI AGG'CCT
Cuts at: 0 246 524 990
Size: 246 278 466
Fragments arranged by size:
466 278 246
StyI C'CwwG_G
Cuts at: 0 917 974 990
Size: 917 57 16
Fragments arranged by size:
917 57 16
TatI w'GTAC_w
Cuts at: 0 569 990
Size: 569 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 287 319 990
Size: 287 32 671
Fragments arranged by size:
671 287 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 975 990
Size: 154 821 15
Fragments arranged by size:
821 154 15
XmaI C'CCGG_G
Cuts at: 0 679 990
Size: 679 311
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaLI AvaI BamHI BanI
BanII BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI HaeII Hin4I HincII
MspA1I NaeI NdeI NgoMIV NspI PasI PflMI PfoI
PstI SacI SacII SalI SbfI SfcI SmaI SmlI
SphI SrfI StuI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BplI BsaAI BsaHI BsaWI BseRI
BsiWI BsmI BsmBI BspEI BspMI BsrDI BssSI BstBI
BstEII BstZ17I BtgZI DraI DraIII EarI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NcoI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SspI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI