Restriction map for
pFN21AE0757
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaHI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI BspEI BspLU11I BspMI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI DraII DraIII
Eam1105I EarI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I MfeI MluI MluNI
MroI MscI MseI MslI MunI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 550 GTCGAC GT^[AC][GT]AC
Alw44I 762 GTGCAC G^TGCAC
Aor51HI 505 AGCGCT AGC^GCT
ApaLI 762 GTGCAC G^TGCAC
AvaII 93 GGTCC G^G[AT]CC
BamHI 302 GGATCC G^GATCC
BfaI 652 CTAG C^TAG
BglI 47 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 735 GCTGAGC GC^T[ACGT]AGC
BpmI 187 CTGGAG CTGGAG(16/14)
Bpu1102I 735 GCTGAGC GC^T[ACGT]AGC
BsaBI 303 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 137 GAGACC GGTCTC(1/5)
BsgI 840 GTGCAG GTGCAG(16/14)
BsiEI 57 CGGCCG CG[GA][CT]^CG
Bsp1407I 569 TGTACA T^GTACA
BspDI 310 ATCGAT AT^CGAT
BspHI 176 TCATGA T^CATGA
BsrFI 658 GCCGGC [GA]^CCGG[CT]
BsrGI 569 TGTACA T^GTACA
BstXI 716 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 735 GCTGAGC GC^T[ACGT]AGC
Cfr10I 658 GCCGGC [GA]^CCGG[CT]
ClaI 310 ATCGAT AT^CGAT
DrdI 541 GACTGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 57 CGGCCG C^GGCCG
EclXI 57 CGGCCG C^GGCCG
Eco47III 505 AGCGCT AGC^GCT
Eco52I 57 CGGCCG C^GGCCG
EcoNI 141 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 550 GTCGAC GT[CT]^[GA]AC
HindII 550 GTCGAC GT[CT]^[GA]AC
HinfI 548 GAGTC G^A[ACGT]TC
MaeI 652 CTAG C^TAG
MaeII 950 ACGT A^CGT
MamI 303 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 379 GAAGA GAAGA(8/7)
NaeI 658 GCCGGC GCC^GGC
NdeI 407 CATATG CA^TATG
NgoMIV 658 GCCGGC G^CCGGC
PflMI 376 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 548 GAGTC GAGTC(4/5)
RcaI 176 TCATGA T^CATGA
SacI 172 GAGCTC GAGCT^C
SalI 550 GTCGAC G^TCGAC
SbfI 744 CCTGCAGG CCTGCA^GG
SmaI 679 CCCGGG CCC^GGG
SphI 647 GCATGC GCATG^C
Sse8387I 744 CCTGCAGG CCTGCA^GG
SspBI 569 TGTACA T^GTACA
Van91I 376 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 679 CCCGGG C^CCGGG
XmaI 679 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 570 GTAC GT^AC
963 GTAC
AocI 247 CCTGAGG CC^T[ACGT]AGG
356 CCTCAGG
BanI 361 GGCACC G^G[CT][GA]CC
790 GGCACC
BsmAI 137 GAGAC GTCTC(1/5)
492 GTCTC
BsrBI 738 GAGCGG CCGCTC(-3/-3)
892 GAGCGG
BssHII 71 GCGCGC G^CGCGC
922 GCGCGC
BstF5I 823 CATCC GGATG(2/0)
575 GGATG
Bsu36I 247 CCTGAGG CC^T[ACGT]AGG
356 CCTCAGG
Eco81I 247 CCTGAGG CC^T[ACGT]AGG
356 CCTCAGG
EcoT14I 917 CCAAGG C^C[AT][AT]GG
974 CCAAGG
FokI 823 CATCC GGATG(9/13)
575 GGATG
HaeII 505 AGCGCT [GA]GCGC^[CT]
727 GGCGCT
KspI 529 CCGCGG CCGC^GG
605 CCGCGG
NspI 486 GCATGT [GA]CATG^[CT]
647 GCATGC
PstI 543 CTGCAG CTGCA^G
745 CTGCAG
RsaI 570 GTAC GT^AC
963 GTAC
SacII 529 CCGCGG CCGC^GG
605 CCGCGG
SfcI 543 CTGCAG C^T[GA][CT]AG
745 CTGCAG
SmlI 703 CTCAAG C^T[CT][GA]AG
862 CTCAAG
StuI 244 AGGCCT AGG^CCT
522 AGGCCT
StyI 917 CCAAGG C^C[AT][AT]GG
974 CCAAGG
TaqI 311 TCGA T^CGA
551 TCGA
Tsp45I 13 GTGAC ^GT[GC]AC
845 GTGAC
TthHB8I 311 TCGA T^CGA
551 TCGA
XcmI 147 CCAGGCCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
968 CCAATGCCAAGGTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaI BanII BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TseI TspRI
XhoII