Gene/Protein Characteristic Table for KIAA1099
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Product ID ORK00741
Accession No AB029022
Description ArfGAP with GTPase domain, ankyrin repeat and PH domain 1, transcript variant 2
Clone name hk07834
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4112 bp)
Predicted protein sequence (864 aa)
Flexi ORF Clone FXC00741
Source Human adult brain
Rouge ID mKIAA1099 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4112 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1126 bp
Genome contig ID gi89161199f_235967595
PolyA signal sequence
(AATAAA,-16)
+----*----+----*----+----*----+----
ATGTGTGAAAATATATTTGAATAAAAGAAGTTCAT
Flanking genome sequence
(731037 - 731086)
----+----*----+----*----+----*----+----*----+----*
AAATATGCATTGATTTTTGTACAGACAAATGGCACCTCTCTTAATTTATA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 236067499 236698630 17 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 864 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAL04172 0 100.0 centaurin gamma...
Homo sapiens
EAW71079 0 99.9 centaurin, gamm...
Homo sapiens
NP_055729 0 99.8 centaurin, gamm...
Homo sapiens
XP_001082744 0 99.4 similar to cent...
Macaca mulatta
NP_001032213 0 97.4 centaurin, gamm...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB075855 2.2e-102 82.0 KIAA1975
D79989 4.6e-48 55.8 KIAA0167
AB051503 4.8e-12 39.8 KIAA1716
AB033075 5.7e-12 31.1 KIAA1249
D26069 3.8e-11 38.7 KIAA0041
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001806 132 153 PR00449 Ras GTPase
IPR001806 171 193 PR00449 Ras GTPase
IPR001806 229 242 PR00449 Ras GTPase
IPR001806 267 289 PR00449 Ras GTPase
IPR001164 628 647 PR00405 Arf GTPase activating protein
IPR001164 647 664 PR00405 Arf GTPase activating protein
IPR001164 668 689 PR00405 Arf GTPase activating protein
IPR002110 776 788 PR01415 Ankyrin
IPR002110 821 833 PR01415 Ankyrin
HMMPfam IPR013684 133 241 PF08477 Miro-like
IPR001849 407 595 PF00169 Pleckstrin-like
IPR001164 616 736 PF01412 Arf GTPase activating protein
IPR002110 775 807 PF00023 Ankyrin
IPR002110 808 833 PF00023 Ankyrin
HMMSmart IPR003577 129 292 SM00173 Ras small GTPase
IPR003579 132 292 SM00175 Ras small GTPase
IPR001849 407 597 SM00233 Pleckstrin-like
IPR001164 616 736 SM00105 Arf GTPase activating protein
IPR002110 775 804 SM00248 Ankyrin
IPR002110 808 839 SM00248 Ankyrin
HMMTigr IPR005225 129 287 TIGR00231 Small GTP-binding protein domain
ProfileScan IPR001849 406 595 PS50003 Pleckstrin-like
IPR001164 616 736 PS50115 Arf GTPase activating protein
IPR002110 743 832 PS50297 Ankyrin
IPR002110 775 807 PS50088 Ankyrin
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CAAAGAGAGGAAATCAGTCGC
Primer_r AGACTAACACATCCACCTCGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f CAAAGAGAGGAAATCAGTCGC
Primer_r AGACTAACACATCCACCTCGG
PCR product length 231 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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