Gene/Protein Characteristic Table for KIAA0812
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK06860
Accession No AB011536
Description cadherin, EGF LAG seven-pass G-type receptor 3
Clone name hg01044
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5832 bp)
Predicted protein sequence (1364 aa)
Source Human adult brain
Rouge ID mKIAA0812 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5832 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1736 bp
Genome contig ID gi89161205r_48548906
PolyA signal sequence
(AATAAA,-15)
+----*----+----*----+----*----+----
AAGCCGCGCTCTGTTTTGGGAATAAACTTCTATAG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAACAGTTGTTGCCTTTTCCTGTTGGGGGCTGGGGAGGTAGGATGGAAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 r 48648906 48664378 24 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 1364 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAA32464 0 100.0 MEGF2 [Homo sap...
Homo sapiens
EAW64914 0 99.6 cadherin, EGF L...
Homo sapiens
Q9NYQ7 0 99.6 Cadherin EGF LA...
Homo sapiens
NP_001398 0 99.6 cadherin EGF LA...
Homo sapiens
O88278 0 93.4 Cadherin EGF LA...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D87469 6.5e-42 43.5 KIAA0279
AB020628 2.4e-16 24.2 KIAA0821
AB018329 3.1e-15 26.2 KIAA0786
AB018311 4.5e-15 29.8 KIAA0768
AB011122 1.4e-09 22.0 KIAA0550
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000832 593 617 PR00249 GPCR
IPR000832 656 679 PR00249 GPCR
IPR000832 694 719 PR00249 GPCR
IPR000832 736 761 PR00249 GPCR
IPR000832 805 826 PR00249 GPCR
HMMPfam IPR006209 2 28 PF00008 EGF-like
IPR006209 32 66 PF00008 EGF-like
IPR002049 124 157 PF00053 EGF-like
IPR001879 173 235 PF02793 Hormone receptor
IPR000203 528 576 PF01825 GPS
IPR000832 588 830 PF00002 GPCR
HMMSmart IPR006210 1 29 SM00181 EGF
IPR006210 31 67 SM00181 EGF
IPR002049 124 169 SM00180 EGF-like
IPR001879 172 239 SM00008 Hormone receptor
IPR000203 528 581 SM00303 GPS
ProfileScan IPR000742 1 26 PS50026 EGF-like
IPR000742 29 67 PS50026 EGF-like
IPR002049 124 171 PS50027 EGF-like
IPR001879 156 234 PS50227 Hormone receptor
IPR000203 529 581 PS50221 GPS
IPR000832 591 827 PS50261 GPCR
ScanRegExp IPR000152 8 19 PS00010 Aspartic acid and asparagine hydroxylation site
IPR013032 17 28 PS00022 EGF-like region
IPR013032 17 32 PS01186 EGF-like region
IPR013032 55 66 PS00022 EGF-like region
IPR013032 55 66 PS01186 EGF-like region
IPR013032 143 154 PS00022 EGF-like region
IPR002049 143 176 PS01248 EGF-like
IPR000832 815 830 PS00650 GPCR

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 592 AVFTHVVVAVSVAALVLTAAILL 614 PRIMARY 23
2 625 GIHANVAAALGVAELLFLLGIHR 647 SECONDARY 23
3 656 AVAILLHYFFLSTFAWLFVQGLH 678 PRIMARY 23
4 694 MRFYHALGWGVPAVLLGLAVGL 715 PRIMARY 22
5 738 FAGPVVLVIVMNGTMFLLAART 759 PRIMARY 22
6 776 LRSSFLLLLLVSASWLFGLLAVN 798 PRIMARY 23
7 808 HAGLCGLQGLAVLLLFCVLNAD 829 PRIMARY 22
Experimental conditions
Primer_f
Primer_r
PCR conditions °C sec °C sec cycles

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name GeneBridge 4
Primer_f TCGGTAAGGAGAGTTTGGGTC
Primer_r CAGACACTCCTCACCCACCAC
PCR product length 124 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp