##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Aor51HI AscI AseI
AsnI Asp718I BclI BfrI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI Eco47III EcoO65I EcoRV
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AgeI 3195 ACCGGT A^CCGGT
Asp700I 4944 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1848 TGCGCA TGC^GCA
BbrPI 1787 CACGTG CAC^GTG
BciVI 757 GGATAC GTATCC(6/5)
BglII 542 AGATCT A^GATCT
BsaAI 1787 CACGTG [CT]AC^GT[GA]
BsaI 5405 GGTCTC GGTCTC(1/5)
BsaWI 3195 ACCGGT [AT]^CCGG[AT]
BspHI 1470 TCATGA T^CATGA
BsrBI 2659 CCGCTC CCGCTC(-3/-3)
BsrDI 2991 GCAATG GCAATG(2/0)
DraI 5194 TTTAAA TTT^AAA
EcoNI 5109 CCTTAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4998 GAATTC G^AATTC
FspI 1848 TGCGCA TGC^GCA
HincII 2390 GTCAAC GT[CT]^[GA]AC
HindII 2390 GTCAAC GT[CT]^[GA]AC
PaeR7I 3603 CTCGAG C^TCGAG
PinAI 3195 ACCGGT A^CCGGT
PmaCI 1787 CACGTG CAC^GTG
PmlI 1787 CACGTG CAC^GTG
RcaI 1470 TCATGA T^CATGA
SfiI 2438 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 3359 TCTAGA T^CTAGA
XhoI 3603 CTCGAG C^TCGAG
XmnI 4944 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3731 GACTCTCTGTC
AspEI 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3731 GACTCTCTGTC
AspI 2714 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3842 GACTCGGTC
BsmI 3464 GAATGC GAATGC(1/-1)
4592 GAATGC
BssSI 2192 CACGAG CACGAG(-5/-1)
3795 CACGAG
EagI 2665 CGGCCG C^GGCCG
3722 CGGCCG
Eam1105I 2996 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3731 GACTCTCTGTC
EclXI 2665 CGGCCG C^GGCCG
3722 CGGCCG
Eco52I 2665 CGGCCG C^GGCCG
3722 CGGCCG
EcoT22I 3466 ATGCAT ATGCA^T
5551 ATGCAT
KspI 344 CCGCGG CCGC^GG
2071 CCGCGG
NsiI 3466 ATGCAT ATGCA^T
5551 ATGCAT
PflFI 2714 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3842 GACTCGGTC
SacII 344 CCGCGG CCGC^GG
2071 CCGCGG
SapI 2014 GCTCTTC GCTCTTC(1/4)
2464 GCTCTTC
SexAI 3771 ACCAGGT A^CC[AT]GGT
4224 ACCTGGT
SphI 2043 GCATGC GCATG^C
4264 GCATGC
Tth111I 2714 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3842 GACTCGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI