Gene/Protein Characteristic Table for KIAA0557
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Product ID ORK00562
Accession No AB011129
Description zinc finger protein 500, transcript variant 1
Clone name hh01334
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5627 bp)
Predicted protein sequence (493 aa)
Flexi ORF Clone FXC00562
Source Human adult brain
Features of the cloned cDNA sequence
Description

Length: 5627 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 4144 bp
Genome contig ID gi51511732r_4638353
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CCAACCTGGGCAACACCGTGAGACCCCATCTCTAC
Flanking genome sequence
(99888 - 99839)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAAAAATTAGCTGGACATGGTGGTGTGCACCTGTAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 16 r 4738241 4756020 5 99.1 Perfect prediction
Features of the protein sequence
Description

Length: 493 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O60304 5.5e-179 100.0 Zinc finger pro...
Homo sapiens
XP_001098209 4e-167 94.2 zinc finger pro...
Macaca mulatta
BAG60301 5.8e-160 100.0 unnamed protein...
Homo sapiens
AAI21015 3e-101 100.0 ZNF500 protein ...
Homo sapiens
AAH04125 1.9e-84 100.0 ZNF500 protein ...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB051497 1.4e-20 41.0 KIAA1710
AB002324 2.4e-20 38.8 KIAA0326
AB075842 3.9e-19 32.4 KIAA1962
AB058777 7.6e-19 59.4 KIAA1874
AB075849 1.5e-18 55.5 KIAA1969
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR007087 338 361 PD000003 Zinc finger
IPR007087 366 389 PD000003 Zinc finger
IPR007087 394 417 PD000003 Zinc finger
IPR007087 422 445 PD000003 Zinc finger
HMMPfam IPR003309 57 152 PF02023 Transcriptional regulator SCAN
IPR001909 237 273 PF01352 KRAB box
IPR007087 338 360 PF00096 Zinc finger
IPR007087 366 388 PF00096 Zinc finger
IPR007087 394 416 PF00096 Zinc finger
IPR007087 422 444 PF00096 Zinc finger
IPR007087 450 472 PF00096 Zinc finger
HMMSmart IPR003309 59 165 SM00431 Transcriptional regulator SCAN
IPR015880 338 360 SM00355 Zinc finger
IPR015880 366 388 SM00355 Zinc finger
IPR015880 394 416 SM00355 Zinc finger
IPR015880 422 444 SM00355 Zinc finger
IPR015880 450 472 SM00355 Zinc finger
ProfileScan IPR003309 63 144 PS50804 Transcriptional regulator SCAN
IPR007087 338 365 PS50157 Zinc finger
IPR007087 366 393 PS50157 Zinc finger
IPR007087 394 421 PS50157 Zinc finger
IPR007087 422 449 PS50157 Zinc finger
IPR007087 450 477 PS50157 Zinc finger
ScanRegExp IPR007087 340 360 PS00028 Zinc finger
IPR007087 368 388 PS00028 Zinc finger
IPR007087 396 416 PS00028 Zinc finger
IPR007087 424 444 PS00028 Zinc finger
IPR007087 452 472 PS00028 Zinc finger
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f AGAGGAGTAACGAGAATTTGC
Primer_r GATTCATAAAGGTCAAAGGCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 16
Experimental conditions
Panel name GeneBridge 4
Primer_f AGAGGAGTAACGAGAATTTGC
Primer_r GATTCATAAAGGTCAAAGGCC
PCR product length 93 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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