##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AccIII      AflII       AgeI        AocI        AscI        
AseI        AsnI        BciVI       BfrI        BglII       
BsaBI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      BstBI       
BstEII      BstPI       Bsu36I      ClaI        CpoI        
DraI        Eco81I      EcoO65I     EcoRV       FseI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        1048  GACGTC               GACGT^C
AccI          731  GTATAC               GT^[AC][GT]AC
AclI         2731  AACGTT               AA^CGTT
AflIII       2435  ACATGT               A^C[GA][CT]GT
AhdI         2238  GACCCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        2238  GACCCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        5562  CCTAGG               C^CTAGG
BamHI         807  GGATCC               G^GATCC
BbeI         5046  GGCGCC               GGCGC^C
BcgI         1561  CGAAAGCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         5562  CCTAGG               C^CTAGG
BsaWI          31  ACCGGA               [AT]^CCGG[AT]
BsmI         2346  GCATTC               GAATGC(1/-1)
BspLU11I     2435  ACATGT               A^CATGT
BsrFI        1447  GCCGGT               [GA]^CCGG[CT]
BssSI         410  CTCGTG               CACGAG(-5/-1)
Bst1107I      731  GTATAC               GTA^TAC
BstZ17I       731  GTATAC               GTA^TAC
Cfr10I       1447  GCCGGT               [GA]^CCGG[CT]
DrdI         1549  GACAGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          278  CGGCCG               C^GGCCG
Eam1105I     2238  GACCCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         278  CGGCCG               C^GGCCG
Eco52I        278  CGGCCG               C^GGCCG
EcoRI        1531  GAATTC               G^AATTC
EcoT22I       757  ATGCAT               ATGCA^T
KasI         5046  GGCGCC               G^GCGCC
NarI         5046  GGCGCC               GG^CGCC
NheI         3697  GCTAGC               G^CTAGC
NsiI          757  ATGCAT               ATGCA^T
NspI         2435  ACATGT               [GA]CATG^[CT]
PshAI        4411  GACGCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2731  AACGTT               AA^CGTT
SacI         1216  GAGCTC               GAGCT^C
SfoI         5046  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I        864  GGTACC               G^GTACC
             3660  GGTACC              
Aor51HI      1203  AGCGCT               AGC^GCT
             1287  AGCGCT              
Asp700I      1201  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1285  GAAGCGCTTC          
Asp718I       864  GGTACC               G^GTACC
             3660  GGTACC              
AviII         288  TGCGCA               TGC^GCA
             4180  TGCGCA              
BglI         4387  GCCCAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5516  GCCAGGCTGGC         
BsiEI         278  CGGCCG               CG[GA][CT]^CG
             1129  CGACCG              
BsmBI         158  GAGACG               CGTCTC(1/5)
             1952  GAGACG              
BstAPI       1272  GCACCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1652  GCACTGGGTGC         
DraIII       1273  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1653  CACTGGGTG           
Eco47III     1203  AGCGCT               AGC^GCT
             1287  AGCGCT              
FspI          288  TGCGCA               TGC^GCA
             4180  TGCGCA              
KpnI          864  GGTACC               GGTAC^C
             3660  GGTACC              
MslI         1331  CACACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3931  CACTCACGTG          
NcoI          994  CCATGG               C^CATGG
             1590  CCATGG              
SmaI          231  CCCGGG               CCC^GGG
             2332  CCCGGG              
TaqI          562  TCGA                 T^CGA
             3152  TCGA                
TthHB8I       562  TCGA                 T^CGA
             3152  TCGA                
XbaI         3625  TCTAGA               T^CTAGA
             5400  TCTAGA              
XmaCI         231  CCCGGG               C^CCGGG
             2332  CCCGGG              
XmaI          231  CCCGGG               C^CCGGG
             2332  CCCGGG              
XmnI         1201  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1285  GAAGCGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       ApoI        AspHI       AspI        AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BclI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflFI       PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XcmI        
XhoII