##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AflII AgeI AocI AscI
AseI AsnI BciVI BfrI BglII
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII BstBI
BstEII BstPI Bsu36I ClaI CpoI
DraI Eco81I EcoO65I EcoRV FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1048 GACGTC GACGT^C
AccI 731 GTATAC GT^[AC][GT]AC
AclI 2731 AACGTT AA^CGTT
AflIII 2435 ACATGT A^C[GA][CT]GT
AhdI 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5562 CCTAGG C^CTAGG
BamHI 807 GGATCC G^GATCC
BbeI 5046 GGCGCC GGCGC^C
BcgI 1561 CGAAAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 5562 CCTAGG C^CTAGG
BsaWI 31 ACCGGA [AT]^CCGG[AT]
BsmI 2346 GCATTC GAATGC(1/-1)
BspLU11I 2435 ACATGT A^CATGT
BsrFI 1447 GCCGGT [GA]^CCGG[CT]
BssSI 410 CTCGTG CACGAG(-5/-1)
Bst1107I 731 GTATAC GTA^TAC
BstZ17I 731 GTATAC GTA^TAC
Cfr10I 1447 GCCGGT [GA]^CCGG[CT]
DrdI 1549 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 278 CGGCCG C^GGCCG
Eam1105I 2238 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 278 CGGCCG C^GGCCG
Eco52I 278 CGGCCG C^GGCCG
EcoRI 1531 GAATTC G^AATTC
EcoT22I 757 ATGCAT ATGCA^T
KasI 5046 GGCGCC G^GCGCC
NarI 5046 GGCGCC GG^CGCC
NheI 3697 GCTAGC G^CTAGC
NsiI 757 ATGCAT ATGCA^T
NspI 2435 ACATGT [GA]CATG^[CT]
PshAI 4411 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2731 AACGTT AA^CGTT
SacI 1216 GAGCTC GAGCT^C
SfoI 5046 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 864 GGTACC G^GTACC
3660 GGTACC
Aor51HI 1203 AGCGCT AGC^GCT
1287 AGCGCT
Asp700I 1201 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1285 GAAGCGCTTC
Asp718I 864 GGTACC G^GTACC
3660 GGTACC
AviII 288 TGCGCA TGC^GCA
4180 TGCGCA
BglI 4387 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5516 GCCAGGCTGGC
BsiEI 278 CGGCCG CG[GA][CT]^CG
1129 CGACCG
BsmBI 158 GAGACG CGTCTC(1/5)
1952 GAGACG
BstAPI 1272 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1652 GCACTGGGTGC
DraIII 1273 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1653 CACTGGGTG
Eco47III 1203 AGCGCT AGC^GCT
1287 AGCGCT
FspI 288 TGCGCA TGC^GCA
4180 TGCGCA
KpnI 864 GGTACC GGTAC^C
3660 GGTACC
MslI 1331 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3931 CACTCACGTG
NcoI 994 CCATGG C^CATGG
1590 CCATGG
SmaI 231 CCCGGG CCC^GGG
2332 CCCGGG
TaqI 562 TCGA T^CGA
3152 TCGA
TthHB8I 562 TCGA T^CGA
3152 TCGA
XbaI 3625 TCTAGA T^CTAGA
5400 TCTAGA
XmaCI 231 CCCGGG C^CCGGG
2332 CCCGGG
XmaI 231 CCCGGG C^CCGGG
2332 CCCGGG
XmnI 1201 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1285 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XcmI
XhoII