Gene/Protein Characteristic Table for KIAA0142
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Product ID ORK00426
Accession No D63476
Description Rho guanine nucleotide exchange factor (GEF) 7, transcript variant 1
Clone name ha01169
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (5032 bp)
Predicted protein sequence (802 aa)
Flexi ORF Clone FXC00426
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0142 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5032 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2618 bp
Genome contig ID gi51511729f_110504086
PolyA signal sequence
(ATTAAA,-26)
+----*----+----*----+----*----+----
GTTGTAATTATTAAACTGATTATTTTTCTTATGTC
Flanking genome sequence
(251995 - 252044)
----+----*----+----*----+----*----+----*----+----*
ACAGAATGTGTCGCTTCGTCTTCTTTGATGTGTTTTCTGTGTGCTTGTTA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 13 f 110604086 110756079 20 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 802 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAX09148 0 99.5 Rho guanine nuc...
Homo sapiens
BAH13248 0 99.7 unnamed protein...
Homo sapiens
AAH50521 0 99.9 Rho guanine nuc...
Homo sapiens
XP_509742 0 97.0 Rho guanine nuc...
Pan troglodytes
XP_001926031 0 94.5 similar to Rho ...
Sus scrofa
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D25304 4.7e-59 59.4 KIAA0006
AB029035 1.9e-08 23.7 KIAA1112
AB033082 4.5e-07 24.5 KIAA1256
AB037836 1e-05 26.1 KIAA1415
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001452 166 216 PD000066 Src homology-3
FPrintScan IPR001452 179 194 PR00452 Src homology-3
IPR001452 196 205 PR00452 Src homology-3
IPR001452 207 219 PR00452 Src homology-3
HMMPfam IPR001452 165 219 PF00018 Src homology-3
IPR000219 253 428 PF00621 DH
IPR001849 458 556 PF00169 Pleckstrin-like
HMMSmart IPR001452 165 220 SM00326 Src homology-3
IPR000219 253 428 SM00325 DH
IPR001849 458 558 SM00233 Pleckstrin-like
ProfileScan IPR001452 162 221 PS50002 Src homology-3
IPR000219 249 429 PS50010 DH
IPR001849 451 556 PS50003 Pleckstrin-like
ScanRegExp IPR001331 377 402 PS00741 Guanine-nucleotide dissociation stimulator
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 13
Experimental conditions
Panel name Genebridge 4
Primer_f GGGTTACTCCTTTGCTCTCAC
Primer_r AACCAGGGCAGGCATTCAAGG
PCR product length 190 bp
PCR conditions 95 °C15 sec64 °C120 sec30 cycles
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