ARHGEF7 ORF clone FHC00426

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Halotag ORF Clone FHC00426

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Clone info.

Species

human

Clone Name

pFN21ASDA0142 Sequence (with vector) Restriction Map

Product ID

FHC00426

Flexi Clone PID

FXC00426

ORF Sequence

DNA sequence (1938 bp)
Protein sequence (646 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383765]

Gene

KIAA0142

Symbol

ARHGEF7

Alias : BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB

Description

Rho guanine nucleotide exchange factor (GEF) 7, transcript variant 1

Original Clone ID

ha01169

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1938 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 646 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9521.1 1.3e-123 100.0 ARHGEF7 CCDS45069.1 2.3e-106 99.8 ARHGEF7 CCDS32009.1 2.4e-106 99.8 ARHGEF7 CCDS45068.1 2.4e-106 99.8 ARHGEF7 CCDS81781.1 5.5e-87 99.8 ARHGEF7 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001452 23 38 PR00452 Src homology-3 domain IPR001452 40 49 PR00452 Src homology-3 domain IPR001452 51 63 PR00452 Src homology-3 domain HMMPfam IPR011511 11 63 PF07653 Variant SH3 IPR000219 97 272 PF00621 Dbl homology (DH) domain IPR001849 311 400 PF00169 Pleckstrin homology domain HMMSmart IPR001452 9 64 SM00326 Src homology-3 domain IPR000219 97 272 SM00325 Dbl homology (DH) domain IPR001849 302 402 SM00233 Pleckstrin homology domain ProfileScan IPR001452 6 65 PS50002 Src homology-3 domain IPR000219 93 273 PS50010 Dbl homology (DH) domain IPR001849 295 400 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003890 646 605477 100.0 100.0 NP_001307780 646 605477 100.0 100.0 NP_001106985 646 605477 100.0 100.0 XP_016876314 646 605477 100.0 100.0 XP_016876313 646 605477 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0142 Download

>KIAA0142  1938 bp
ATGACCGACAATAGCAACAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACC
AATGAGGACGAGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAG
GGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTG
CGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAGCCCT
CCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAAT
ATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTA
CGGCCATTGCAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAAT
CTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAG
TTGCCCGAAGCTCAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAA
ACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACAC
AGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTG
CTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAA
GAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATG
GCTGCCTTCAAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAG
CTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGC
AACGTCACTTACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAA
AGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGT
GGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGAC
AGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGTG
TCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAG
GTCACGTCTGTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCC
TCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCC
GCCTACCACACGCTGCCCCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGG
GGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCG
CCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAG
ACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTC
GCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAA
GCTTACTGCACCAGCGCCAAAACAAGGCAAACACTCAATTCAAGTTCACGCAAAGAATCT
GCTCCACAAGTTTTGCTTCCAGAAGAAGAGAAAATTATAGTGGAAGAAACTAAAAGTAAT
GGTCAGACAGTGATAGAAGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAGGATGAA
GTTCAAGAATTAAGACAGGACAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGA
GCCCGCAAAGACCTGGAGAAGCTGGTGAGGAAAGTCCTGAAGAACATGAATGATCCTGCC
TGGGATGAGACCAATCTA

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0142 Download

>KIAA0142  646 aa
MTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYV
REVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYL
RPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMK
TLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLK
ELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLG
NVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLED
SENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLP
SHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPA
PPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIE
AYCTSAKTRQTLNSSSRKESAPQVLLPEEEKIIVEETKSNGQTVIEEKSLVDTVYALKDE
VQELRQDNKKMKKSLEEEQRARKDLEKLVRKVLKNMNDPAWDETNL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0142 Download

>pFN21ASDA0142  6667 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGACCGACAATAGCAA
CAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTC
CTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGG
CACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAG
CGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAGCCCTCCCAAAGGATTTGATAC
GACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAATATTTTAGAAACAGAAAA
TGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTGCAGACCAG
TGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTC
TTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTCAGCA
GAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTA
TTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGA
GTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTGCTGACCACGGGCCTGAG
CAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAAGAGCTCGAGAGACACAT
GGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTCAAAAACCT
TTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGA
AGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTC
CCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTT
CCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAGGG
AAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAATCATAGAAA
TGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGTGTCGTGCAACAACCAGCA
GGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCTGTGGGAAA
CCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCC
GTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCC
CCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCC
GAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCGCCTCCCCTCCGGCCCTC
AGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAGACCATGAAAAAGCTGCT
GCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTCGCGTCCCGGAAAAGCAC
AGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGCACCAGCGC
CAAAACAAGGCAAACACTCAATTCAAGTTCACGCAAAGAATCTGCTCCACAAGTTTTGCT
TCCAGAAGAAGAGAAAATTATAGTGGAAGAAACTAAAAGTAATGGTCAGACAGTGATAGA
AGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAGGATGAAGTTCAAGAATTAAGACA
GGACAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGAGCCCGCAAAGACCTGGA
GAAGCTGGTGAGGAAAGTCCTGAAGAACATGAATGATCCTGCCTGGGATGAGACCAATCT
ATACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT