Gene/Protein Characteristic Table for KIAA1256
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Product ID ORK00207
Accession No AB033082
Description intersectin 2, transcript variant 3
Clone name hh15293
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5938 bp)
Predicted protein sequence (1676 aa)
Flexi ORF Clone FXC00207
Source Human adult brain
Rouge ID mKIAA1256 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5938 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 760 bp
Genome contig ID gi89161199r_24179239
PolyA signal sequence
(AATAAA,-25)
+----*----+----*----+----*----+----
ACAGAAAGAGAATAAAGACATATGTTTATGGATGG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AACTTCGTCAAAGTCTGACTGATTTACTTTCTATATGAAAGCTGCTTTTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 r 24279239 24436808 39 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1676 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAF63600 0 100.0 intersectin 2 [...
Homo sapiens
BAG10443 0 100.0 intersectin-2 [...
synthetic construct
EAX00753 0 99.9 intersectin 2, ...
Homo sapiens
AAI46780 0 100.0 Intersectin 2 [...
Homo sapiens
XP_532890 0 94.4 similar to inte...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002380 2.2e-05 28.6 KIAA0382
AB002378 9.9e-05 26.5 KIAA0380
D63476 0.00063 24.5 KIAA0142
AB029035 0.00093 23.4 KIAA1112
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR002048 255 312 PD000012 Calcium-binding EF-hand
IPR001452 741 793 PD000066 Src homology-3
IPR001452 881 930 PD000066 Src homology-3
IPR001452 965 1013 PD000066 Src homology-3
IPR001452 1043 1091 PD000066 Src homology-3
IPR001452 1110 1160 PD000066 Src homology-3
FPrintScan IPR000108 1050 1069 PR00499 Neutrophil cytosol factor 2
IPR001452 1109 1119 PR00452 Src homology-3
IPR000108 1111 1131 PR00499 Neutrophil cytosol factor 2
IPR001452 1123 1138 PR00452 Src homology-3
IPR000108 1131 1147 PR00499 Neutrophil cytosol factor 2
IPR001452 1140 1149 PR00452 Src homology-3
IPR000108 1147 1160 PR00499 Neutrophil cytosol factor 2
IPR001452 1151 1163 PR00452 Src homology-3
IPR000008 1565 1577 PR00360 C2 calcium-dependent membrane targeting
IPR000008 1589 1602 PR00360 C2 calcium-dependent membrane targeting
HMMPfam IPR002048 64 92 PF00036 Calcium-binding EF-hand
IPR002048 287 315 PF00036 Calcium-binding EF-hand
IPR001452 741 795 PF00018 Src homology-3
IPR011511 881 933 PF07653 Variant SH3
IPR001452 963 1016 PF00018 Src homology-3
IPR001452 1035 1094 PF00018 Src homology-3
IPR001452 1109 1163 PF00018 Src homology-3
IPR000219 1192 1373 PF00621 DH
IPR001849 1414 1523 PF00169 Pleckstrin-like
IPR000008 1550 1631 PF00168 C2 calcium-dependent membrane targeting
HMMSmart IPR000261 21 115 SM00027 EPS15 homology (EH)
IPR002048 64 92 SM00054 Calcium-binding EF-hand
IPR000261 243 338 SM00027 EPS15 homology (EH)
IPR002048 287 315 SM00054 Calcium-binding EF-hand
IPR001452 739 796 SM00326 Src homology-3
IPR001452 880 934 SM00326 Src homology-3
IPR001452 963 1017 SM00326 Src homology-3
IPR001452 1035 1095 SM00326 Src homology-3
IPR001452 1109 1164 SM00326 Src homology-3
IPR000219 1192 1373 SM00325 DH
IPR001849 1414 1525 SM00233 Pleckstrin-like
IPR000008 1549 1646 SM00239 C2 calcium-dependent membrane targeting
ProfileScan IPR000261 28 116 PS50031 EPS15 homology (EH)
IPR002048 60 95 PS50222 Calcium-binding EF-hand
IPR000261 250 339 PS50031 EPS15 homology (EH)
IPR002048 283 318 PS50222 Calcium-binding EF-hand
IPR001452 736 797 PS50002 Src homology-3
IPR001452 877 935 PS50002 Src homology-3
IPR001452 960 1018 PS50002 Src homology-3
IPR001452 1032 1096 PS50002 Src homology-3
IPR001452 1106 1165 PS50002 Src homology-3
IPR000219 1188 1374 PS50010 DH
IPR001849 1413 1523 PS50003 Pleckstrin-like
IPR000008 1549 1631 PS50004 C2 calcium-dependent membrane targeting
ScanRegExp IPR002048 73 85 PS00018 Calcium-binding EF-hand
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CCCTCCGAACAGACAACATTA
Primer_r GTAGCTTCAATGACATGCACC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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