##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

Acc65I      AflII       AgeI        AscI        AseI        
AsnI        Asp718I     BbrPI       BclI        BfrI        
BsiWI       Bsp1407I    BspDI       BspHI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
DrdI        EcoRI       FbaI        FseI        HpaI        
KpnI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
PvuI        RcaI        SalI        SbfI        SexAI       
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          168  GTCTAC               GT^[AC][GT]AC
AcsI          287  GAATTT               [GA]^AATT[CT]
AhdI         2691  GACTAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2486  GTGCAC               G^TGCAC
AocI         3482  CCTTAGG              CC^T[ACGT]AGG
Aor51HI       145  AGCGCT               AGC^GCT
ApaI         3581  GGGCCC               GGGCC^C
ApaLI        2486  GTGCAC               G^TGCAC
ApoI          287  GAATTT               [GA]^AATT[CT]
AspEI        2691  GACTAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3818  TGCGCA               TGC^GCA
AvrII        1482  CCTAGG               C^CTAGG
BalI         3854  TGGCCA               TGG^CCA
BamHI        4502  GGATCC               G^GATCC
BbvCI        1927  CCTCAGC              CCTCAGC(-5/-2)
BcgI           23  GCAGCGGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2246  GGATAC               GTATCC(6/5)
BglII        3260  AGATCT               A^GATCT
BlnI         1482  CCTAGG               C^CTAGG
BsaBI         270  GATTTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         620  CGGCCG               CG[GA][CT]^CG
BssSI        1128  CTCGTG               CACGAG(-5/-1)
BstAPI       1628  GCAGGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3673  GGTGACC              G^GT[ACGT]ACC
BstPI        3673  GGTGACC              G^GT[ACGT]ACC
BstXI        4616  CCAAGGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3482  CCTTAGG              CC^T[ACGT]AGG
CpoI          624  CGGACCG              CG^G[AT]CCG
EagI          620  CGGCCG               C^GGCCG
Eam1105I     2691  GACTAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         620  CGGCCG               C^GGCCG
Eco47III      145  AGCGCT               AGC^GCT
Eco52I        620  CGGCCG               C^GGCCG
Eco81I       3482  CCTTAGG              CC^T[ACGT]AGG
EcoNI         711  CCTCCCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3673  GGTGACC              G^GT[ACGT]ACC
EcoRV        1435  GATATC               GAT^ATC
FspI         3818  TGCGCA               TGC^GCA
MamI          270  GATTTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        3854  TGGCCA               TGG^CCA
MscI         3854  TGGCCA               TGG^CCA
NaeI          129  GCCGGC               GCC^GGC
NcoI         1249  CCATGG               C^CATGG
NgoMIV        129  GCCGGC               G^CCGGC
PaeR7I       1197  CTCGAG               C^TCGAG
PshAI        2473  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         624  CGGACCG              CG^G[AT]CCG
SacI         1194  GAGCTC               GAGCT^C
SapI          704  GAAGAGC              GCTCTTC(1/4)
ScaI         3151  AGTACT               AGT^ACT
SfiI         4459  GGCCATGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SwaI         4941  ATTTAAAT             ATTT^AAAT
XcmI         3585  CCAGGCTGGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1197  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII         564  GACGTC               GACGT^C
             2417  GACGTC              
AccIII       1291  TCCGGA               T^CCGGA
             1336  TCCGGA              
AclI         3519  AACGTT               AA^CGTT
             4640  AACGTT              
AflIII       1384  ACATGT               A^C[GA][CT]GT
             2745  ACATGT              
Asp700I      4220  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4405  GAAGTATTTC          
AspI         3072  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4955  GACAATGTC           
BbeI         2519  GGCGCC               GGCGC^C
             4909  GGCGCC              
BseAI        1291  TCCGGA               T^CCGGA
             1336  TCCGGA              
BspEI        1291  TCCGGA               T^CCGGA
             1336  TCCGGA              
BspLU11I     1384  ACATGT               A^CATGT
             2745  ACATGT              
HincII       4916  GTTGAC               GT[CT]^[GA]AC
             4969  GTTGAC              
HindII       4916  GTTGAC               GT[CT]^[GA]AC
             4969  GTTGAC              
KasI         2519  GGCGCC               G^GCGCC
             4909  GGCGCC              
KspI          113  CCGCGG               CCGC^GG
             3569  CCGCGG              
MroI         1291  TCCGGA               T^CCGGA
             1336  TCCGGA              
NarI         2519  GGCGCC               GG^CGCC
             4909  GGCGCC              
PflFI        3072  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4955  GACAATGTC           
PflMI        1843  CCATCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1879  CCAAGCCCTGG         
Psp1406I     3519  AACGTT               AA^CGTT
             4640  AACGTT              
SacII         113  CCGCGG               CCGC^GG
             3569  CCGCGG              
SfoI         2519  GGCGCC               GGC^GCC
             4909  GGCGCC              
TaqI         1198  TCGA                 T^CGA
             4403  TCGA                
Tth111I      3072  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4955  GACAATGTC           
TthHB8I      1198  TCGA                 T^CGA
             4403  TCGA                
Van91I       1843  CCATCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1879  CCAAGCCCTGG         
XbaI          893  TCTAGA               T^CTAGA
             2315  TCTAGA              
XmnI         4220  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4405  GAAGTATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII