NPFFR1 ORF clone pFN21AE9540

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Halotag ORF Clone pFN21AE9540

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Clone info.

Species

human

Clone Name

pFN21AE9540 Sequence (with vector) Restriction Map

Product ID

FHC03177

ORF Sequence

DNA sequence (1290 bp)
Protein sequence (430 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9540

Symbol

NPFFR1

Alias : GPR147, NPFF1, NPFF1R1, OT7T022

Description

neuropeptide FF receptor 1

Original Clone ID

cp99540

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1290 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 430 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS53539.1 3e-141 100.0 NPFFR1 CCDS3552.1 1.7e-63 56.4 NPFFR2 CCDS47072.1 1.7e-63 56.4 NPFFR2 CCDS3551.1 1.9e-63 56.4 NPFFR2 CCDS34089.1 1.5e-22 33.4 NPY1R The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR005396 4 19 PR01571 Neuropeptide FF receptor IPR005395 29 42 PR01570 Neuropeptide FF receptor IPR000276 45 69 PR00237 GPCR IPR005395 54 66 PR01570 Neuropeptide FF receptor IPR000276 78 99 PR00237 GPCR IPR005395 93 110 PR01570 Neuropeptide FF receptor IPR000276 123 145 PR00237 GPCR IPR005395 142 156 PR01570 Neuropeptide FF receptor IPR000276 157 178 PR00237 GPCR IPR005396 178 201 PR01571 Neuropeptide FF receptor IPR000276 217 240 PR00237 GPCR IPR005396 240 256 PR01571 Neuropeptide FF receptor IPR005396 260 270 PR01571 Neuropeptide FF receptor IPR000276 268 292 PR00237 GPCR IPR005395 307 319 PR01570 Neuropeptide FF receptor IPR000276 311 337 PR00237 GPCR IPR005395 334 344 PR01570 Neuropeptide FF receptor IPR005396 356 372 PR01571 Neuropeptide FF receptor IPR005396 379 408 PR01571 Neuropeptide FF receptor IPR005396 414 428 PR01571 Neuropeptide FF receptor HMMPfam IPR000276 60 329 PF00001 GPCR ProfileScan IPR017452 60 329 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 45 MFIVAYALIFLLCMVGNTLVCFI 67 PRIMARY 23 2 78 TNMFILNLAVSDLLVGIFCMPTT 100 SECONDARY 23 3 119 SGLVQGMSVSASVFTLVAIAVER 141 SECONDARY 23 4 158 ALVTIAVIWALALLIMCPSAVTL 180 PRIMARY 23 5 215 VYTTVLFSHIYLAPLALIVVMYA 237 PRIMARY 23 6 269 VHMLVMVALFFTLSWLPLWALLL 291 PRIMARY 23 7 299 SAPQLHLVTVYAFPFAHWLAFFN 321 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071429 430 607448 100.0 100.0 NP_444264 420 607449 56.4 81.9 NP_001138228 423 607449 56.4 81.9 NP_004876 522 607449 56.4 81.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9540 Download

>KIEE9540  1290 bp
ATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAGTTGGCCCCTAAGTCAGAATGGGACT
AACACTGAGGCCACCCCGGCTACAAACCTCACCTTCTCCTCCTACTATCAGCACACCTCC
CCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCTCATCTTCCTGCTCTGCATGGTGGGC
AACACCCTGGTCTGTTTCATCGTGCTCAAGAACCGGCACATGCATACTGTCACCAACATG
TTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGTGGGCATCTTCTGCATGCCCACCACC
CTTGTGGACAACCTCATCACTGGGTGGCCCTTCGACAATGCCACATGCAAGATGAGCGGC
TTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTTCACACTGGTGGCCATTGCTGTGGAA
AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTGCGGAAGGCGCTCGTC
ACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCATCATGTGTCCCTCGGCCGTCACGCTG
ACCGTCACCCGTGAGGAGCACCACTTCATGGTGGACGCCCGCAACCGCTCCTACCCGCTC
TACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCATGCGCAGGGTCTACACCACTGTGCTC
TTCTCGCACATCTACCTGGCGCCGCTGGCGCTCATCGTGGTCATGTACGCCCGCATCGCG
CGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGGGGGCGAGGAGGCTGCGGACCCGCGA
GCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCTGGTCATGGTGGCGCTGTTCTTCACG
CTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCTCATCGACTACGGGCAGCTCAGCGCG
CCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCCCTTCGCGCACTGGCTGGCCTTCTTC
AACAGCAGCGCCAACCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTC
CAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCCGTCGGGGAGCCACAAGGAGGCCTAC
TCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGTCTTCGTGGTGGTGCGGCCCAGCGAC
TCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGGGGCCCCCAGGCCCGGCCGCCTCCCG
CTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCCCAGGGAAGGGCCTGGCTGCTCCCAC
CTGCCCCTCACCATTCCAGCCTGGGATATC

ORF Protein

Cloned ORF protein sequence for pFN21AE9540 Download

>KIEE9540  430 aa
MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVG
NTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSG
LVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTL
TVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIA
RKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSA
PQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAY
SERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSH
LPLTIPAWDI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9540 Download

>pFN21AE9540  5995 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGGGGAGCCCTCCCAGCCTCCCAACAGCAG
TTGGCCCCTAAGTCAGAATGGGACTAACACTGAGGCCACCCCGGCTACAAACCTCACCTT
CTCCTCCTACTATCAGCACACCTCCCCTGTGGCGGCCATGTTCATTGTGGCCTATGCGCT
CATCTTCCTGCTCTGCATGGTGGGCAACACCCTGGTCTGTTTCATCGTGCTCAAGAACCG
GCACATGCATACTGTCACCAACATGTTCATCCTCAACCTGGCTGTCAGTGACCTGCTGGT
GGGCATCTTCTGCATGCCCACCACCCTTGTGGACAACCTCATCACTGGGTGGCCCTTCGA
CAATGCCACATGCAAGATGAGCGGCTTGGTGCAGGGCATGTCTGTGTCGGCTTCCGTTTT
CACACTGGTGGCCATTGCTGTGGAAAGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAA
GCTGACCCTGCGGAAGGCGCTCGTCACCATCGCCGTCATCTGGGCCCTGGCGCTGCTCAT
CATGTGTCCCTCGGCCGTCACGCTGACCGTCACCCGTGAGGAGCACCACTTCATGGTGGA
CGCCCGCAACCGCTCCTACCCGCTCTACTCCTGCTGGGAGGCCTGGCCCGAGAAGGGCAT
GCGCAGGGTCTACACCACTGTGCTCTTCTCGCACATCTACCTGGCGCCGCTGGCGCTCAT
CGTGGTCATGTACGCCCGCATCGCGCGCAAGCTCTGCCAGGCCCCGGGCCCGGCCCCCGG
GGGCGAGGAGGCTGCGGACCCGCGAGCATCGCGGCGCAGAGCGCGCGTGGTGCACATGCT
GGTCATGGTGGCGCTGTTCTTCACGCTGTCCTGGCTGCCGCTCTGGGCGCTGCTGCTGCT
CATCGACTACGGGCAGCTCAGCGCGCCGCAGCTGCACCTGGTCACCGTCTACGCCTTCCC
CTTCGCGCACTGGCTGGCCTTCTTCAACAGCAGCGCCAACCCCATCATCTACGGCTACTT
CAACGAGAACTTCCGCCGCGGCTTCCAGGCCGCCTTCCGCGCCCGCCTCTGCCCGCGCCC
GTCGGGGAGCCACAAGGAGGCCTACTCCGAGCGGCCCGGCGGGCTTCTGCACAGGCGGGT
CTTCGTGGTGGTGCGGCCCAGCGACTCCGGGCTGCCCTCTGAGTCGGGCCCTAGCAGTGG
GGCCCCCAGGCCCGGCCGCCTCCCGCTGCGGAATGGGCGGGTGGCTCACCACGGCTTGCC
CAGGGAAGGGCCTGGCTGCTCCCACCTGCCCCTCACCATTCCAGCCTGGGATATCGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT