################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DrdI Eam1105I Eco47III Eco81I EcoRI EcoT14I FbaI FseI HincII HindII HindIII HpaI KpnI MaeII MamI MboI MfeI MflI MluI MroI MseI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI Sau3AI SbfI ScaI SfcI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 705 GTAC GT^AC AflIII 236 ACATGT A^C[GA][CT]GT AvaII 771 GGACC G^G[AT]CC AviII 635 TGCGCA TGC^GCA BalI 404 TGGCCA TGG^CCA BanI 678 GGCGCC G^G[CT][GA]CC BbeI 678 GGCGCC GGCGC^C BbsI 1114 GTCTTC GAAGAC(2/6) BcgI 759 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1166 CTAG C^TAG BlpI 891 GCTCAGC GC^T[ACGT]AGC Bpu1102I 891 GCTCAGC GC^T[ACGT]AGC BpuAI 1114 GTCTTC GAAGAC(2/6) BspLU11I 236 ACATGT A^CATGT BsrDI 408 CATTGC GCAATG(2/0) BsrFI 212 ACCGGC [GA]^CCGG[CT] BstEII 915 GGTCACC G^GT[ACGT]ACC BstF5I 243 CATCC GGATG(2/0) BstPI 915 GGTCACC G^GT[ACGT]ACC CelII 891 GCTCAGC GC^T[ACGT]AGC Cfr10I 212 ACCGGC [GA]^CCGG[CT] DraIII 318 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 658 CTCTTC CTCTTC(1/4) EcoNI 462 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 915 GGTCACC G^GT[ACGT]ACC EcoRV 1285 GATATC GAT^ATC EcoT22I 220 ATGCAT ATGCA^T FokI 243 CATCC GGATG(9/13) FspI 635 TGCGCA TGC^GCA HgaI 574 GACGC GACGC(5/10) KasI 678 GGCGCC G^GCGCC Ksp632I 658 CTCTTC CTCTTC(1/4) KspI 1011 CCGCGG CCGC^GG MaeI 1166 CTAG C^TAG MluNI 404 TGGCCA TGG^CCA MscI 404 TGGCCA TGG^CCA NarI 678 GGCGCC GG^CGCC NsiI 220 ATGCAT ATGCA^T PflMI 561 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 904 CAGCTG CAG^CTG RsaI 705 GTAC GT^AC SacII 1011 CCGCGG CCGC^GG SapI 657 GCTCTTC GCTCTTC(1/4) SexAI 911 ACCTGGT A^CC[AT]GGT SfoI 678 GGCGCC GGC^GCC SmlI 205 CTCAAG C^T[CT][GA]AG Van91I 561 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 643 GTCTAC GT^[AC][GT]AC 922 GTCTAC AcyI 574 GACGCC G[GA]^CG[CT]C 678 GGCGCC Alw44I 436 GTGCAC G^TGCAC 805 GTGCAC ApaLI 436 GTGCAC G^TGCAC 805 GTGCAC BglI 70 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1089 GCCCGGCGGGC BsaHI 574 GACGCC G[GA]^CG[CT]C 678 GGCGCC BsiEI 527 CGGCCG CG[GA][CT]^CG 1188 CGGCCG BsmFI 55 GGGAC GGGAC(10/14) 521 GTCCC BspMI 266 ACCTGC ACCTGC(4/8) 1259 ACCTGC BstAPI 278 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 722 GCAAGCTCTGC BstXI 296 CCACCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 407 CCATTGCTGTGG DsaI 1011 CCGCGG C^C[GA][CT]GG 1224 CCACGG EagI 527 CGGCCG C^GGCCG 1188 CGGCCG EclXI 527 CGGCCG C^GGCCG 1188 CGGCCG Eco52I 527 CGGCCG C^GGCCG 1188 CGGCCG Hin1I 574 GACGCC G[GA]^CG[CT]C 678 GGCGCC HinfI 1138 GACTC G^A[ACGT]TC 1156 GAGTC PleI 1138 GACTC GAGTC(4/5) 1156 GAGTC SmaI 738 CCCGGG CCC^GGG 751 CCCGGG SphI 287 GCATGC GCATG^C 632 GCATGC StuI 614 AGGCCT AGG^CCT 1073 AGGCCT TaqI 332 TCGA T^CGA 878 TCGA TthHB8I 332 TCGA T^CGA 878 TCGA XcmI 131 CCATGTTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 293 CCACCACCCTTGTGG XmaCI 738 CCCGGG C^CCGGG 751 CCCGGG XmaI 738 CCCGGG C^CCGGG 751 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI ApaI AspHI AvaI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrBI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI Sau96I ScrFI SfaNI TseI Tsp45I TspRI