(Circular) (Six-base) MAPSORT of: pFN21AE9540.seq Check: 8964 from: 1 to: 5995 pFN21AE9540 5995 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3273 3273 Size: 5995 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2649 2928 3338 1937 Size: 712 279 410 4594 Fragments arranged by size: 4594 712 410 279 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 5442 5710 1632 Size: 2426 1384 268 1917 Fragments arranged by size: 2426 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 2241 4894 5871 1203 Size: 1038 2653 977 1327 Fragments arranged by size: 2653 1327 1038 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 2306 2405 2657 1166 Size: 1140 99 252 4504 Fragments arranged by size: 4504 1140 252 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 3738 5310 3738 Size: 1572 4423 ApaI G_GGCC'C Cuts at: 2506 2750 3170 3184 2506 Size: 244 420 14 5317 Fragments arranged by size: 5317 420 244 14 ApaLI G'TGCA_C Cuts at: 2441 2810 4052 5208 2441 Size: 369 1242 1156 3228 Fragments arranged by size: 3228 1242 1156 369 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2627 2743 2756 3320 1870 Size: 73 684 116 13 564 4545 Fragments arranged by size: 4545 684 564 116 73 13 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 3316 4771 618 Size: 325 217 627 896 633 1455 1842 Fragments arranged by size: 1842 1455 896 633 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2506 2750 3170 3184 3314 1391 Size: 628 487 244 420 14 130 4072 Fragments arranged by size: 4072 628 487 420 244 130 14 BbeI G_GCGC'C Cuts at: 2687 2687 Size: 5995 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3112 928 Size: 971 1213 3811 Fragments arranged by size: 3811 1213 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2753 2787 4186 4220 1903 Size: 34 816 34 1399 34 3678 Fragments arranged by size: 3678 1399 816 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BfrBI ATG'CAT Cuts at: 2227 2227 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 3100 4606 Size: 107 122 71 631 605 409 1019 1506 Cuts at: 4606 136 Size: 1525 Fragments arranged by size: 1525 1506 1019 631 605 409 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 2897 3412 2897 Size: 515 5480 Bme1580I G_kGCm'C Cuts at: 1790 2445 2506 2750 2814 3170 3184 4056 5212 Size: 655 61 244 64 356 14 872 1156 Cuts at: 5212 1790 Size: 2573 Fragments arranged by size: 2573 1156 872 655 356 244 64 61 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 3726 4684 Size: 416 53 83 399 24 917 1392 958 Cuts at: 4684 442 Size: 1753 Fragments arranged by size: 1753 1392 958 917 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3100 3100 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4655 1239 Size: 3416 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 4120 4985 5283 2195 Size: 1925 865 298 2907 Fragments arranged by size: 2907 1925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4658 882 Size: 617 3159 2219 Fragments arranged by size: 3159 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2580 2684 3802 4184 Size: 53 83 186 936 1047 104 1118 382 Cuts at: 4184 4819 275 Size: 635 1451 Fragments arranged by size: 1451 1118 1047 936 635 382 186 104 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2573 2780 1186 Size: 906 481 207 4401 Fragments arranged by size: 4401 906 481 207 BseYI C'CCAG_C Cuts at: 2022 2613 3137 5198 5694 2022 Size: 591 524 2061 496 2323 Fragments arranged by size: 2323 2061 591 524 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 2897 3092 1163 Size: 573 654 507 195 4066 Fragments arranged by size: 4066 654 573 507 195 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2535 3196 3445 4206 5234 1540 Size: 275 186 534 661 249 761 1028 2301 Fragments arranged by size: 2301 1028 761 661 534 275 249 186 BsiHKAI G_wGCw'C Cuts at: 2211 2445 2565 2664 2814 4056 4141 5212 2211 Size: 234 120 99 150 1242 85 1071 2994 Fragments arranged by size: 2994 1242 1071 234 150 120 99 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2211 2445 2506 2565 2664 2750 Size: 399 229 192 234 61 59 99 86 Cuts at: 2750 2814 3170 3184 3314 4056 4141 5212 1391 Size: 64 356 14 130 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 234 229 192 130 99 86 85 64 61 59 14 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 3273 3350 844 Size: 1436 993 77 3489 Fragments arranged by size: 3489 1436 993 77 BsrBI CCG'CTC Cuts at: 1394 1823 2361 2592 2602 2860 3091 3882 1394 Size: 429 538 231 10 258 231 791 3507 Fragments arranged by size: 3507 791 538 429 258 231 231 10 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 4490 4664 65 Size: 1183 1164 2078 174 1396 Fragments arranged by size: 2078 1396 1183 1164 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 4639 4826 1700 Size: 110 407 2422 187 2869 Fragments arranged by size: 2869 2422 407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 2723 2801 2901 1925 Size: 798 78 100 5019 Fragments arranged by size: 5019 798 100 78 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 2289 2733 794 Size: 1495 444 4056 Fragments arranged by size: 4056 1495 444 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 2920 1184 Size: 1736 4259 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2419 2308 Size: 111 5884 BstYI r'GATC_y Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990 Size: 1353 367 385 17 1452 86 12 346 Cuts at: 5990 1972 Size: 1977 Fragments arranged by size: 1977 1452 1353 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3016 3229 513 Size: 552 939 1012 213 3279 Fragments arranged by size: 3279 1012 939 552 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2473 2704 2772 487 Size: 683 56 1247 231 68 3710 Fragments arranged by size: 3710 1247 683 231 68 56 BtsI GCAGTG_nn' Cuts at: 3181 3511 4305 4325 3181 Size: 330 794 20 4851 Fragments arranged by size: 4851 794 330 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2287 2322 529 Size: 35 1723 35 4202 Fragments arranged by size: 4202 1723 35 35 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DraIII CAC_nnn'GTG Cuts at: 2328 2328 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2409 2532 Size: 54 1425 50 275 18 303 276 123 Cuts at: 2532 3193 3442 4331 5960 8 Size: 661 249 889 1629 43 Fragments arranged by size: 1629 1425 889 661 303 276 275 249 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2532 3193 3442 1537 Size: 275 720 661 249 4090 Fragments arranged by size: 4090 720 661 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 3925 1353 Size: 1316 1256 3423 Fragments arranged by size: 3423 1316 1256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3002 4582 4956 5102 387 Size: 1064 138 1413 1580 374 146 1280 Fragments arranged by size: 1580 1413 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4058 4655 5442 5710 1239 Size: 393 2426 597 787 268 1524 Fragments arranged by size: 2426 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2503 2740 3167 3180 3181 3248 2503 Size: 237 427 13 1 67 5250 Fragments arranged by size: 5250 427 237 67 13 1 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 EcoRV GAT'ATC Cuts at: 3292 3292 Size: 5995 FspI TGC'GCA Cuts at: 2642 4501 2642 Size: 1859 4136 HaeII r_GCGC'y Cuts at: 1678 2480 2513 2687 2696 2834 2870 2976 5142 Size: 802 33 174 9 138 36 106 2166 Cuts at: 5142 1678 Size: 2531 Fragments arranged by size: 2531 2166 802 174 138 106 36 33 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KasI G'GCGC_C Cuts at: 2683 2683 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5109 5293 1305 Size: 3804 184 2007 Fragments arranged by size: 3804 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 3594 Fragments arranged by size: 3594 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2306 2381 2405 2417 2657 3953 Size: 648 1074 66 75 24 12 240 1296 Cuts at: 3953 4312 4471 518 Size: 359 159 2042 Fragments arranged by size: 2042 1296 1074 648 359 240 159 75 66 24 12 MspA1I CmG'CkG Cuts at: 1606 2433 2689 2911 3018 3205 3411 4088 5236 Size: 827 256 222 107 187 206 677 1148 Cuts at: 5236 5481 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 827 677 256 245 222 206 187 107 NarI GG'CG_CC Cuts at: 2684 2684 Size: 5995 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NsiI A_TGCA'T Cuts at: 2229 2229 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2227 2245 2296 2352 2380 2641 2818 Size: 97 998 18 51 56 28 261 177 Cuts at: 2818 3353 4898 1132 Size: 535 1545 2229 Fragments arranged by size: 2229 1545 998 535 261 177 97 56 51 28 18 PasI CC'CwG_GG Cuts at: 1348 3240 1348 Size: 1892 4103 PciI A'CATG_T Cuts at: 2241 4894 2241 Size: 2653 3342 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 1108 Size: 1464 4531 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 2502 2746 3166 3180 2502 Size: 244 420 14 5317 Fragments arranged by size: 5317 420 244 14 PstI C_TGCA'G Cuts at: 830 3347 830 Size: 2517 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2911 2911 Size: 5995 SacII CC_GC'GG Cuts at: 3019 3019 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SapI GCTCTTCn'nnn_ Cuts at: 2669 2669 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SexAI A'CCwGG_T Cuts at: 2916 2916 Size: 5995 SfcI C'TryA_G Cuts at: 826 1045 3343 4478 5159 5350 826 Size: 219 2298 1135 681 191 1471 Fragments arranged by size: 2298 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2685 2685 Size: 5995 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 2745 2758 3322 1872 Size: 873 13 564 4545 Fragments arranged by size: 4545 873 564 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 4099 5000 5262 820 Size: 197 926 267 1889 901 262 1553 Fragments arranged by size: 1889 1553 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 2296 2641 3353 1132 Size: 1164 345 712 3774 Fragments arranged by size: 3774 1164 712 345 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2621 3080 1517 Size: 1104 459 4432 Fragments arranged by size: 4432 1104 459 StyI C'CwwG_G Cuts at: 513 1065 2004 3434 513 Size: 552 939 1430 3074 Fragments arranged by size: 3074 1430 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 4022 4039 4192 4377 4822 325 Size: 1255 736 1706 17 153 185 445 1498 Fragments arranged by size: 1706 1498 1255 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2305 2143 Size: 162 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 2743 2756 3320 1870 Size: 873 13 564 4545 Fragments arranged by size: 4545 873 564 13 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI