KRT3 ORF clone pFN21AE6743

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6743

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Clone info.

Species

human

Clone Name

pFN21AE6743 Sequence (with vector) Restriction Map

Product ID

FHC30537

ORF Sequence

DNA sequence (1884 bp)
Protein sequence (628 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6743

Symbol

KRT3

Alias : CK3, K3, MECD2

Description

keratin 3, type II

Original Clone ID

oc96743

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1884 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 628 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44895.1 3e-105 100.0 KRT3 CCDS8838.1 2.1e-63 74.3 KRT76 CCDS8830.1 5.4e-56 64.6 KRT5 CCDS8835.1 2.3e-55 64.8 KRT2 CCDS8836.1 2.4e-55 64.8 KRT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003054 200 208 PR01276 Type II keratin IPR003054 227 239 PR01276 Type II keratin IPR003054 276 289 PR01276 Type II keratin IPR003054 290 309 PR01276 Type II keratin IPR003054 401 418 PR01276 Type II keratin IPR003054 452 467 PR01276 Type II keratin HMMPfam IPR016044 197 512 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_476429 628 122100,148043 100.0 100.0 NP_056932 638 616671 74.3 99.8 NP_000414 639 146800,600194 64.8 96.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6743 Download

>KIEE6743  1884 bp
ATGAGCAGACAAGCCAGCAAGACATCTGGTGGCGGGAGCCAGGGTTTCTCCGGCCGCTCT
GCTGTGGTCTCCGGCAGCAGCAGGATGAGCTGTGTGGCCCACTCTGGGGGAGCTGGCGGA
GGGGCCTATGGCTTCCGGAGCGGAGCAGGTGGCTTTGGCAGTCGCAGCCTCTACAACCTG
GGCGGCAACAAGAGCATCTCCATCAGCGTGGCAGCTGGCGGCTCCCGGGCTGGAGGCTTT
GGGGGAGGGCGGAGCAGCTGTGCCTTTGCAGGTGGCTATGGAGGTGGCTTTGGGAGCGGC
TATGGAGGTGGCTTTGGTGGTGGCTTTGGTGGTGGCAGAGGAATGGGAGGTGGCTTTGGT
GGAGCTGGTGGCTTTGGAGGGGCTGGTGGCTTTGGAGGGGCTGGTGGCTTTGGTGGTCCT
GGTGGCTTTGGTGGGTCTGGTGGCTTTGGTGGGCCTGGCAGCTTGGGCAGTCCTGGTGGC
TTTGGCCCTGGGGGCTTTCCTGGGGGAATTCAGGAAGTGACTATCAACCAGAGTCTCCTG
CAGCCCCTCAATGTGGAGATCGACCCCCAGATTGGGCAAGTAAAGGCCCAGGAGCGGGAA
CAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATTGACAAGGTGCGGTTCCTGGAG
CAACAGAACAAAGTCCTGGAGACCAAGTGGAACCTGCTCCAGCAGCAGGGCACAAGTTCC
ATCTCAGGCACAAACAACCTTGAGCCTCTTTTTGAGAATCACATCAACTACCTGCGGAGC
TACCTGGACAACATCCTCGGGGAGAGAGGGCGCCTGGACTCTGAGCTGAAGAACATGGAG
GACCTGGTGGAAGACTTCAAGAAGAAATATGAGGATGAAATCAATAAACGTACAGCTGCT
GAGAATGAATTTGTGACTCTGAAGAAGGATGTGGACAGTGCCTATATGAACAAGGTGGAG
CTTCAGGCCAAAGTGGATGCCTTGATAGATGAGATCGACTTCTTAAGGACCCTCTACGAC
GCTGAGCTATCTCAGATGCAGAGCCACATCAGTGACACATCTGTGGTGCTGTCCATGGAC
AATAATCGCTCCCTGGACCTGGACAGCATCATTGCTGAAGTTCGTGCACAGTATGAGGAT
ATCGCTCAGAGAAGCAAGGCCGAAGCTGAGGCCCTGTACCAGACCAAGTTGGGGGAGCTG
CAGACCACGGCTGGCAGGCATGGGGATGACCTAAGAAATACCAAGAGCGAGATCATAGAG
CTCAACAGAATGATCCAGAGGCTGCGGGCAGAGATCGAGGGTGTCAAGAAGCAGAATGCC
AACCTGCAGACGGCCATTGCCGAGGCCGAGCAGCATGGAGAGATGGCCCTCAAGGATGCC
AATGCCAAGCTCCAAGAGCTGCAGGCTGCTCTACAGCAGGCGAAGGATGACCTGGCGCGG
CTGCTACGTGACTACCAGGAGCTGATGAATGTCAAGCTGGCCCTGGACGTGGAGATCGCC
ACCTACCGCAAGCTGCTGGAGGGCGAGGAGTACAGGATGTCTGGAGAGTGTCCGAGTGCT
GTCAGCATCTCCGTGGTCAGCAGCAGCACGACTTCCGCCTCCGCAGGTGGCTATGGAGGA
GGTTACGGCGGAGGCATGGGCGGTGGTTTAGGAGGTGGCTTCAGTGCGGGCGGCGGCTCA
GGCAGTGGCTTTGGCCGGGGAGGCGGCGGTGGAATCGGCGGTGGATTTGGCGGCGGCAGC
AGCGGTTTCAGCGGTGGCAGCGGCTTTGGCTCCATCTCTGGCGCCCGCTATGGAGTCAGT
GGCGGGGGCTTCAGCTCGGCCAGCAACCGGGGCGGCAGCATCAAGTTCTCCCAGTCCTCC
CAGTCCTCCCAGCGCTACTCCAGA

ORF Protein

Cloned ORF protein sequence for pFN21AE6743 Download

>KIEE6743  628 aa
MSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNL
GGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFG
GAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLL
QPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSS
ISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAA
ENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMD
NNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIE
LNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLAR
LLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGG
GYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVS
GGGFSSASNRGGSIKFSQSSQSSQRYSR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6743 Download

>pFN21AE6743  6589 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCAGACAAGCCAGCAAGACATCTGGTGGCGG
GAGCCAGGGTTTCTCCGGCCGCTCTGCTGTGGTCTCCGGCAGCAGCAGGATGAGCTGTGT
GGCCCACTCTGGGGGAGCTGGCGGAGGGGCCTATGGCTTCCGGAGCGGAGCAGGTGGCTT
TGGCAGTCGCAGCCTCTACAACCTGGGCGGCAACAAGAGCATCTCCATCAGCGTGGCAGC
TGGCGGCTCCCGGGCTGGAGGCTTTGGGGGAGGGCGGAGCAGCTGTGCCTTTGCAGGTGG
CTATGGAGGTGGCTTTGGGAGCGGCTATGGAGGTGGCTTTGGTGGTGGCTTTGGTGGTGG
CAGAGGAATGGGAGGTGGCTTTGGTGGAGCTGGTGGCTTTGGAGGGGCTGGTGGCTTTGG
AGGGGCTGGTGGCTTTGGTGGTCCTGGTGGCTTTGGTGGGTCTGGTGGCTTTGGTGGGCC
TGGCAGCTTGGGCAGTCCTGGTGGCTTTGGCCCTGGGGGCTTTCCTGGGGGAATTCAGGA
AGTGACTATCAACCAGAGTCTCCTGCAGCCCCTCAATGTGGAGATCGACCCCCAGATTGG
GCAAGTAAAGGCCCAGGAGCGGGAACAGATCAAGACCCTCAACAACAAGTTTGCCTCCTT
CATTGACAAGGTGCGGTTCCTGGAGCAACAGAACAAAGTCCTGGAGACCAAGTGGAACCT
GCTCCAGCAGCAGGGCACAAGTTCCATCTCAGGCACAAACAACCTTGAGCCTCTTTTTGA
GAATCACATCAACTACCTGCGGAGCTACCTGGACAACATCCTCGGGGAGAGAGGGCGCCT
GGACTCTGAGCTGAAGAACATGGAGGACCTGGTGGAAGACTTCAAGAAGAAATATGAGGA
TGAAATCAATAAACGTACAGCTGCTGAGAATGAATTTGTGACTCTGAAGAAGGATGTGGA
CAGTGCCTATATGAACAAGGTGGAGCTTCAGGCCAAAGTGGATGCCTTGATAGATGAGAT
CGACTTCTTAAGGACCCTCTACGACGCTGAGCTATCTCAGATGCAGAGCCACATCAGTGA
CACATCTGTGGTGCTGTCCATGGACAATAATCGCTCCCTGGACCTGGACAGCATCATTGC
TGAAGTTCGTGCACAGTATGAGGATATCGCTCAGAGAAGCAAGGCCGAAGCTGAGGCCCT
GTACCAGACCAAGTTGGGGGAGCTGCAGACCACGGCTGGCAGGCATGGGGATGACCTAAG
AAATACCAAGAGCGAGATCATAGAGCTCAACAGAATGATCCAGAGGCTGCGGGCAGAGAT
CGAGGGTGTCAAGAAGCAGAATGCCAACCTGCAGACGGCCATTGCCGAGGCCGAGCAGCA
TGGAGAGATGGCCCTCAAGGATGCCAATGCCAAGCTCCAAGAGCTGCAGGCTGCTCTACA
GCAGGCGAAGGATGACCTGGCGCGGCTGCTACGTGACTACCAGGAGCTGATGAATGTCAA
GCTGGCCCTGGACGTGGAGATCGCCACCTACCGCAAGCTGCTGGAGGGCGAGGAGTACAG
GATGTCTGGAGAGTGTCCGAGTGCTGTCAGCATCTCCGTGGTCAGCAGCAGCACGACTTC
CGCCTCCGCAGGTGGCTATGGAGGAGGTTACGGCGGAGGCATGGGCGGTGGTTTAGGAGG
TGGCTTCAGTGCGGGCGGCGGCTCAGGCAGTGGCTTTGGCCGGGGAGGCGGCGGTGGAAT
CGGCGGTGGATTTGGCGGCGGCAGCAGCGGTTTCAGCGGTGGCAGCGGCTTTGGCTCCAT
CTCTGGCGCCCGCTATGGAGTCAGTGGCGGGGGCTTCAGCTCGGCCAGCAACCGGGGCGG
CAGCATCAAGTTCTCCCAGTCCTCCCAGTCCTCCCAGCGCTACTCCAGAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT