################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AclI AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfaI BglII BlnI BsaBI BsgI BsiWI BsmBI BsmFI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 1436 CGCG CG^CG AccIII 134 TCCGGA T^CCGGA AflII 1002 CTTAAG C^TTAAG Alw44I 1124 GTGCAC G^TGCAC AlwI 1272 GATCC GGATC(4/5) Aor51HI 1871 AGCGCT AGC^GCT ApaLI 1124 GTGCAC G^TGCAC BanII 1258 GAGCTC G[GA]GC[CT]^C BbsI 850 GAAGAC GAAGAC(2/6) BbvCI 1165 GCTGAGG CCTCAGC(-5/-2) BfrI 1002 CTTAAG C^TTAAG BlpI 1021 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1021 GCTGAGC GC^T[ACGT]AGC BpuAI 850 GAAGAC GAAGAC(2/6) BsaAI 1445 TACGTG [CT]AC^GT[GA] BsaWI 134 TCCGGA [AT]^CCGG[AT] BseAI 134 TCCGGA T^CCGGA BsiEI 51 CGGCCG CG[GA][CT]^CG BsmI 1314 GAATGC GAATGC(1/-1) BspEI 134 TCCGGA T^CCGGA BstUI 1436 CGCG CG^CG BstXI 200 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1021 GCTGAGC GC^T[ACGT]AGC EagI 51 CGGCCG C^GGCCG EclXI 51 CGGCCG C^GGCCG Eco47III 1871 AGCGCT AGC^GCT Eco52I 51 CGGCCG C^GGCCG EcoRI 506 GAATTC G^AATTC EcoRV 1138 GATATC GAT^ATC EcoT14I 1073 CCATGG C^C[AT][AT]GG HgaI 1018 GACGC GACGC(5/10) MroI 134 TCCGGA T^CCGGA MseI 1003 TTAA T^TAA MvnI 1436 CGCG CG^CG NcoI 1073 CCATGG C^CATGG SacI 1258 GAGCTC GAGCT^C SexAI 842 ACCTGGT A^CC[AT]GGT SmaI 224 CCCGGG CCC^GGG StyI 1073 CCATGG C^C[AT][AT]GG Tru9I 1003 TTAA T^TAA XmaCI 224 CCCGGG C^CCGGG XmaI 224 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 506 GAATTC [GA]^AATT[CT] 907 GAATTT AcyI 809 GGCGCC G[GA]^CG[CT]C 1780 GGCGCC ApoI 506 GAATTC [GA]^AATT[CT] 907 GAATTT AspHI 1124 GTGCAC G[AT]GC[AT]^C 1258 GAGCTC AvaI 224 CCCGGG C^[CT]CG[GA]G 796 CTCGGG BanI 809 GGCGCC G^G[CT][GA]CC 1780 GGCGCC BbeI 809 GGCGCC GGCGC^C 1780 GGCGCC BglI 485 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1694 GCCGGGGAGGC BsaHI 809 GGCGCC G[GA]^CG[CT]C 1780 GGCGCC BsaI 679 GAGACC GGTCTC(1/5) 66 GGTCTC BseRI 1525 GAGGAG GAGGAG(10/8) 1616 GAGGAG BsiHKAI 1124 GTGCAC G[AT]GC[AT]^C 1258 GAGCTC BsoBI 224 CCCGGG C^[CT]CG[GA]G 796 CTCGGG BsrDI 1110 CATTGC GCAATG(2/0) 1335 CATTGC BsrI 1851 CCAGT ACTGG(1/-1) 1860 CCAGT Hin1I 809 GGCGCC G[GA]^CG[CT]C 1780 GGCGCC KasI 809 GGCGCC G^GCGCC 1780 GGCGCC NarI 809 GGCGCC GG^CGCC 1780 GGCGCC PpuMI 839 AGGACCT [GA]G^G[AT]CC[CT] 1006 AGGACCC SfoI 809 GGCGCC GGC^GCC 1780 GGCGCC TfiI 756 GAATC G^A[AT]TC 1712 GAATC Tsp509I 507 AATT ^AATT 908 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BspMI BsrBI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I TspRI TthHB8I