(Circular) (Six-base) MAPSORT of: pFN21AE6743.seq Check: 5835 from: 1 to: 6589 pFN21AE6743 6589 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2141 2264 3599 2141 Size: 123 1335 5131 Fragments arranged by size: 5131 1335 123 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2852 2945 2951 3141 3649 3799 4652 6036 Size: 1220 93 6 190 508 150 853 1384 Cuts at: 6036 6304 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1220 853 508 268 190 150 93 6 AfeI AGC'GCT Cuts at: 3878 3878 Size: 6589 AflII C'TTAA_G Cuts at: 820 1017 3007 820 Size: 197 1990 4402 Fragments arranged by size: 4402 1990 197 AflIII A'CryG_T Cuts at: 1203 5488 6465 1203 Size: 4285 977 1327 Fragments arranged by size: 4285 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 3054 3065 1166 Size: 1888 11 4690 Fragments arranged by size: 4690 1888 11 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AlwNI CAG_nnn'CTG Cuts at: 2903 3286 3466 4332 5904 2903 Size: 383 180 866 1572 3588 Fragments arranged by size: 3588 1572 866 383 180 ApaLI G'TGCA_C Cuts at: 3129 4646 5802 3129 Size: 1517 1156 3916 Fragments arranged by size: 3916 1517 1156 ApoI r'AATT_y Cuts at: 1493 2511 2912 3898 4124 4276 6385 1493 Size: 1018 401 986 226 152 2109 1697 Fragments arranged by size: 2109 1697 1018 986 401 226 152 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2229 2801 3914 1870 Size: 73 286 572 1113 4545 Fragments arranged by size: 4545 1113 572 286 73 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2814 3785 3910 5365 618 Size: 325 217 627 1027 971 125 1455 1842 Fragments arranged by size: 1842 1455 1027 971 627 325 217 125 BanII G_rGCy'C Cuts at: 1391 3267 3908 1391 Size: 1876 641 4072 Fragments arranged by size: 4072 1876 641 BbeI G_GCGC'C Cuts at: 2818 3789 2818 Size: 971 5618 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 928 Size: 971 963 4655 Fragments arranged by size: 4655 971 963 BbvCI CC'TCA_GC Cuts at: 3171 3171 Size: 6589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2496 3705 5200 Size: 107 122 71 631 605 824 1209 1495 Cuts at: 5200 136 Size: 1525 Fragments arranged by size: 1525 1495 1209 824 631 605 122 107 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 3027 4006 3027 Size: 979 5610 Bme1580I G_kGCm'C Cuts at: 1790 2717 3133 4650 5806 1790 Size: 927 416 1517 1156 2573 Fragments arranged by size: 2573 1517 1156 927 416 BmgBI CAC'GTC Cuts at: 1318 3493 1318 Size: 2175 4414 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3850 3859 4320 Size: 416 53 83 399 24 2433 9 461 Cuts at: 4320 5278 442 Size: 958 1753 Fragments arranged by size: 2433 1753 958 461 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2256 2681 2687 2702 3542 3567 3868 5249 Size: 1017 425 6 15 840 25 301 1381 Cuts at: 5249 1239 Size: 2579 Fragments arranged by size: 2579 1381 1017 840 425 301 25 15 6 Bpu10I CC'TnA_GC Cuts at: 3171 3682 3171 Size: 511 6078 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 3359 4714 5579 5877 2765 Size: 594 1355 865 298 3477 Fragments arranged by size: 3477 1355 865 594 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2077 2678 5252 882 Size: 617 578 601 2574 2219 Fragments arranged by size: 2574 2219 617 601 578 BsaAI yAC'GTr Cuts at: 493 1204 3452 4380 493 Size: 711 2248 928 2702 Fragments arranged by size: 2702 2248 928 711 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2815 3786 4396 4778 Size: 53 83 186 936 1282 971 610 382 Cuts at: 4778 5413 275 Size: 635 1451 Fragments arranged by size: 1451 1282 971 936 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 5022 5420 5694 5841 6252 2139 Size: 2883 398 274 147 411 2476 Fragments arranged by size: 2883 2476 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2032 2062 2248 2278 2675 Size: 30 165 30 629 30 186 30 397 Cuts at: 2675 2705 1178 Size: 30 5062 Fragments arranged by size: 5062 629 397 186 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3545 3636 1186 Size: 2359 91 4139 Fragments arranged by size: 4139 2359 91 BseYI C'CCAG_C Cuts at: 3873 5792 6288 3873 Size: 1919 496 4174 Fragments arranged by size: 4174 1919 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6016 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 4039 4800 5828 1540 Size: 275 186 58 1980 761 1028 2301 Fragments arranged by size: 2301 1980 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 3133 3267 4650 4735 5806 3133 Size: 134 1383 85 1071 3916 Fragments arranged by size: 3916 1383 1071 134 85 BsmI GAATG_Cn' Cuts at: 3325 4100 4193 3325 Size: 775 93 5721 Fragments arranged by size: 5721 775 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2717 3133 3267 3908 4650 4735 5806 Size: 399 927 416 134 641 742 85 1071 Cuts at: 5806 1391 Size: 2174 Fragments arranged by size: 2174 1071 927 742 641 416 399 134 85 BspEI T'CCGG_A Cuts at: 2139 6252 2139 Size: 4113 2476 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2141 2264 2706 2784 3336 3599 3944 844 Size: 1297 123 442 78 552 263 345 3489 Fragments arranged by size: 3489 1297 552 442 345 263 123 78 BsrBI CCG'CTC Cuts at: 1394 1823 2061 2145 2301 2599 4476 1394 Size: 429 238 84 156 298 1877 3507 Fragments arranged by size: 3507 1877 429 298 238 156 84 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 3339 5084 5258 65 Size: 1183 1866 225 1745 174 1396 Fragments arranged by size: 1866 1745 1396 1183 225 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6589 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstXI CCAn_nnnn'nTGG Cuts at: 2212 2212 Size: 6589 BstYI r'GATC_y Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584 Size: 1947 367 385 17 1452 86 12 346 Cuts at: 6584 1972 Size: 1977 Fragments arranged by size: 1977 1947 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3078 3210 3576 513 Size: 552 2013 132 366 3526 Fragments arranged by size: 3526 2013 552 366 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 3694 4105 4899 4919 3694 Size: 411 794 20 5364 Fragments arranged by size: 5364 794 411 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 3336 3697 Size: 54 1425 50 275 18 226 1280 361 Cuts at: 3697 3822 4036 4925 6554 8 Size: 125 214 889 1629 43 Fragments arranged by size: 1629 1425 1280 889 361 275 226 214 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2056 4036 1537 Size: 275 244 1980 4090 Fragments arranged by size: 4090 1980 275 244 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2136 2269 3589 3648 5176 5550 Size: 1064 138 547 133 1320 59 1528 374 Cuts at: 5550 5696 387 Size: 146 1280 Fragments arranged by size: 1528 1320 1280 1064 547 374 146 138 133 59 EcoICRI GAG'CTC Cuts at: 3265 3265 Size: 6589 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2681 2687 2702 2852 2945 2951 Size: 393 624 425 6 15 150 93 6 Cuts at: 2951 3141 3542 3567 3649 3799 3868 4652 5249 Size: 190 401 25 82 150 69 784 597 Cuts at: 5249 6036 6304 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 784 624 597 425 401 393 268 190 150 150 93 82 69 25 15 6 6 EcoO109I rG'GnC_Cy Cuts at: 2127 2845 3012 3175 2127 Size: 718 167 163 5541 Fragments arranged by size: 5541 718 167 163 EcoRI G'AATT_C Cuts at: 2511 3898 4276 2511 Size: 1387 378 4824 Fragments arranged by size: 4824 1387 378 EcoRV GAT'ATC Cuts at: 3145 3145 Size: 6589 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2818 3789 3880 5736 1678 Size: 1140 971 91 1856 2531 Fragments arranged by size: 2531 1856 1140 971 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5230 5262 5304 5336 1373 Size: 32 135 32 3658 32 42 32 2626 Fragments arranged by size: 3658 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 2814 3785 2814 Size: 971 5618 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5703 5887 1305 Size: 4398 184 2007 Fragments arranged by size: 4398 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4767 Fragments arranged by size: 4767 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 3054 3065 3228 3575 4547 4906 Size: 648 1044 844 11 163 347 972 359 Cuts at: 4906 5065 518 Size: 159 2042 Fragments arranged by size: 2042 1044 972 844 648 359 347 163 159 11 MspA1I CmG'CkG Cuts at: 1606 2219 2262 2900 3747 3756 3765 4005 4682 Size: 613 43 638 847 9 9 240 677 Cuts at: 4682 5830 6075 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 638 613 245 240 43 9 9 NarI GG'CG_CC Cuts at: 2815 3786 2815 Size: 971 5618 NcoI C'CATG_G Cuts at: 513 1065 3078 513 Size: 552 2013 4024 Fragments arranged by size: 4024 2013 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3947 5492 1132 Size: 97 2718 1545 2229 Fragments arranged by size: 2718 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2492 1348 Size: 1144 5445 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PfoI T'CCnGG_A Cuts at: 2658 2679 2658 Size: 21 6568 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2845 3012 2845 Size: 167 6422 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PstI C_TGCA'G Cuts at: 830 2547 3207 3333 3408 3941 830 Size: 1717 660 126 75 533 3478 Fragments arranged by size: 3478 1717 660 533 126 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2219 2262 2900 2219 Size: 43 638 5908 Fragments arranged by size: 5908 638 43 SacI G_AGCT'C Cuts at: 3267 3267 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SexAI A'CCwGG_T Cuts at: 2847 2847 Size: 6589 SfcI C'TryA_G Cuts at: 826 1045 2543 3203 3329 3404 3416 3937 5072 Size: 219 1498 660 126 75 12 521 1135 Cuts at: 5072 5753 5944 826 Size: 681 191 1471 Fragments arranged by size: 1498 1471 1135 681 660 521 219 191 126 75 12 SfoI GGC'GCC Cuts at: 2816 3787 2816 Size: 971 5618 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 2231 3916 1872 Size: 359 1685 4545 Fragments arranged by size: 4545 1685 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 3007 3374 4693 5594 5856 Size: 197 926 801 263 367 1319 901 262 Cuts at: 5856 820 Size: 1553 Fragments arranged by size: 1553 1319 926 901 801 367 263 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 3078 4028 513 Size: 552 2013 950 3074 Fragments arranged by size: 3074 2013 950 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4616 4633 4786 4971 5416 325 Size: 1255 3036 17 153 185 445 1498 Fragments arranged by size: 3036 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3534 4835 6547 Size: 274 80 33 51 1443 1557 1301 1712 Cuts at: 6547 96 Size: 138 Fragments arranged by size: 1712 1557 1443 1301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 2248 2280 2675 2707 6549 6581 2080 Size: 32 136 32 395 32 3842 32 2088 Fragments arranged by size: 3842 2088 395 136 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 2229 3914 1870 Size: 359 1685 4545 Fragments arranged by size: 4545 1685 359 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI