DOC2B ORF clone pFN21AE0591

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Halotag ORF Clone pFN21AE0591

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Clone info.

Species

human

Clone Name

pFN21AE0591 Sequence (with vector) Restriction Map

Product ID

FHC03997

ORF Sequence

DNA sequence (1236 bp)
Protein sequence (412 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590049]

Gene

KIEE0591

Symbol

DOC2B

Alias : DOC2BL

Description

double C2-like domains, beta

Original Clone ID

oc01291

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1236 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 412 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS73934.1 4.8e-91 99.8 DOC2B CCDS31904.1 3e-25 62.2 RPH3A CCDS44979.1 3.1e-25 62.2 RPH3A CCDS10666.1 3.2e-22 61.3 DOC2A CCDS11922.1 7.6e-14 32.4 SYT4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001565 270 285 PR00399 Synaptotagmin IPR001565 285 298 PR00399 Synaptotagmin IPR020477 298 310 PR00360 C2 region IPR020477 327 340 PR00360 C2 region IPR001565 342 357 PR00399 Synaptotagmin IPR020477 351 359 PR00360 C2 region IPR001565 362 372 PR00399 Synaptotagmin HMMPfam IPR000008 143 231 PF00168 C2 calcium-dependent membrane targeting IPR000008 283 370 PF00168 C2 calcium-dependent membrane targeting HMMSmart IPR000008 142 247 SM00239 C2 calcium-dependent membrane targeting IPR000008 282 396 SM00239 C2 calcium-dependent membrane targeting ProfileScan IPR018029 141 232 PS50004 C2 membrane targeting protein IPR018029 283 371 PS50004 C2 membrane targeting protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003576 412 604568 99.8 100.0 XP_011533665 387 604568 93.7 100.0 NP_055769 690 612159 62.2 92.5 NP_001137326 694 612159 62.2 92.5 XP_011536350 694 612159 62.2 92.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0591 Download

>KIEE0591  1236 bp
ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATC
GACGTGTGCCCCGGCCCCATCCGTCCCATCAAGCAGATCTCCGACTACTTCCCCCGCTTC
CCGCGGGGCCTGCCCCCGGACGCCGGGCCCCGAGCCGCTGCACCCCCGGACGCCCCCGCG
CGCCCGGCTGTGGCCGGTGCCGGCCGCCGCAGCCCCTCCGACGGCGCCCGCGAGGACGAC
GAGGATGTGGACCAGCTCTTCGGAGCCTACGGCTCCAGCCCGGGCCCCAGCCCGGGTCCC
AGCCCCGCGCGGCCGCCAGCCAAGCCGCCGGAGGACGAGCCGGACGCCGACGGCTACGAG
TCGGACGACTGCACTGCCCTGGGCACGCTGGACTTCAGCCTGCTGTATGACCAGGAGAAC
AACGCCCTCCACTGCACCATCACCAAGGCCAAGGGCCTGAAGCCAATGGACCACAATGGG
CTGGCAGACCCCTACGTCAAGCTGCACCTGCTGCCAGGAGCCAGTAAGGCAAATAAGCTC
AGAACAAAAACTCTCCGTAACACTCTGAACCCCACATGGAACGAGACCCTCACTTACTAC
GGGATCACAGATGAAGACATGATCCGCAAGACCCTGCGGATCTCTGTGTGTGACGAGGAC
AAATTCCGGCACAATGAGTTCATCGGGGAGACACGTGTGCCCCTGAAGAAGCTGAAACCC
AACCACACCAAGACCTTCAACATCTGCCTGGAGAAGCAGCTGCCGGTGGACAAGACTGAA
GACAAGTCCCTGGAGGAGCGGGGCCGCATCCTCATCTCCCTCAAGTACAGCTCACAGAAG
CAAGGCCTGCTGGTAGGCATCGTGCGGTGCGCCCACCTGGCCGCCATGGACGCCAACGGC
TACTCGGACCCCTACGTGAAAACATACCTGAGGCCAGATGTGGACAAGAAATCCAAACAT
AAGACAGCGGTGAAGAAAAAAACCCTGAACCCGGAGTTTAATGAGGAGTTCTGTTACGAG
ATCAAGCATGGGGACCTGGCCAAGAAGTCCCTGGAGGTCACCGTTTGGGATTACGACATT
GGAAAATCCAACGATTTCATTGGTGGTGTGGTTCTGGGCATCCACGCCAAGGGGGAGCGC
CTGAAGCACTGGTTTGACTGCCTGAAGAACAAGGACAAGCGCATCGAGCGCTGGCACACG
CTCACCAGCGAGCTCCCAGGGGCTGTGCTCAGCGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE0591 Download

>KIEE0591  412 aa
MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPDAGPRAAAPPDAPA
RPAVAGAGRRSPSDGAREDDEDVDQLFGAYGSSPGPSPGPSPARPPAKPPEDEPDADGYE
SDDCTALGTLDFSLLYDQENNALHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKL
RTKTLRNTLNPTWNETLTYYGITDEDMIRKTLRISVCDEDKFRHNEFIGETRVPLKKLKP
NHTKTFNICLEKQLPVDKTEDKSLEERGRILISLKYSSQKQGLLVGIVRCAHLAAMDANG
YSDPYVKTYLRPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKSLEVTVWDYDI
GKSNDFIGGVVLGIHAKGERLKHWFDCLKNKDKRIERWHTLTSELPGAVLSD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0591 Download

>pFN21AE0591  5941 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCAT
CAGCATCCAGGAGCATATGGCCATCGACGTGTGCCCCGGCCCCATCCGTCCCATCAAGCA
GATCTCCGACTACTTCCCCCGCTTCCCGCGGGGCCTGCCCCCGGACGCCGGGCCCCGAGC
CGCTGCACCCCCGGACGCCCCCGCGCGCCCGGCTGTGGCCGGTGCCGGCCGCCGCAGCCC
CTCCGACGGCGCCCGCGAGGACGACGAGGATGTGGACCAGCTCTTCGGAGCCTACGGCTC
CAGCCCGGGCCCCAGCCCGGGTCCCAGCCCCGCGCGGCCGCCAGCCAAGCCGCCGGAGGA
CGAGCCGGACGCCGACGGCTACGAGTCGGACGACTGCACTGCCCTGGGCACGCTGGACTT
CAGCCTGCTGTATGACCAGGAGAACAACGCCCTCCACTGCACCATCACCAAGGCCAAGGG
CCTGAAGCCAATGGACCACAATGGGCTGGCAGACCCCTACGTCAAGCTGCACCTGCTGCC
AGGAGCCAGTAAGGCAAATAAGCTCAGAACAAAAACTCTCCGTAACACTCTGAACCCCAC
ATGGAACGAGACCCTCACTTACTACGGGATCACAGATGAAGACATGATCCGCAAGACCCT
GCGGATCTCTGTGTGTGACGAGGACAAATTCCGGCACAATGAGTTCATCGGGGAGACACG
TGTGCCCCTGAAGAAGCTGAAACCCAACCACACCAAGACCTTCAACATCTGCCTGGAGAA
GCAGCTGCCGGTGGACAAGACTGAAGACAAGTCCCTGGAGGAGCGGGGCCGCATCCTCAT
CTCCCTCAAGTACAGCTCACAGAAGCAAGGCCTGCTGGTAGGCATCGTGCGGTGCGCCCA
CCTGGCCGCCATGGACGCCAACGGCTACTCGGACCCCTACGTGAAAACATACCTGAGGCC
AGATGTGGACAAGAAATCCAAACATAAGACAGCGGTGAAGAAAAAAACCCTGAACCCGGA
GTTTAATGAGGAGTTCTGTTACGAGATCAAGCATGGGGACCTGGCCAAGAAGTCCCTGGA
GGTCACCGTTTGGGATTACGACATTGGAAAATCCAACGATTTCATTGGTGGTGTGGTTCT
GGGCATCCACGCCAAGGGGGAGCGCCTGAAGCACTGGTTTGACTGCCTGAAGAACAAGGA
CAAGCGCATCGAGCGCTGGCACACGCTCACCAGCGAGCTCCCAGGGGCTGTGCTCAGCGA
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T