(Circular) (Six-base) MAPSORT of: pFN21AE0591.seq Check: 1094 from: 1 to: 5941 pFN21AE0591 5941 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2520 2520 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2383 2483 2729 2802 3167 3188 4004 5388 Size: 751 100 246 73 365 21 816 1384 Cuts at: 5388 5656 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 816 751 365 268 246 100 73 21 AfeI AGC'GCT Cuts at: 3194 3194 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2067 2696 2698 4840 5817 1203 Size: 864 629 2 2142 977 1327 Fragments arranged by size: 2142 1327 977 864 629 2 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2654 1184 Size: 1470 4471 AlwNI CAG_nnn'CTG Cuts at: 3227 3684 5256 3227 Size: 457 1572 3912 Fragments arranged by size: 3912 1572 457 ApaI G_GGCC'C Cuts at: 2154 2291 2154 Size: 137 5804 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2666 3250 3476 3628 5737 1493 Size: 1173 584 226 152 2109 1697 Fragments arranged by size: 2109 1697 1173 584 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2154 2284 2296 3266 1870 Size: 73 211 130 12 970 4545 Fragments arranged by size: 4545 970 211 130 73 12 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2228 3262 4717 618 Size: 325 217 627 414 27 1034 1455 1842 Fragments arranged by size: 1842 1455 1034 627 414 325 217 27 BanII G_rGCy'C Cuts at: 1391 2154 2291 3219 3260 1391 Size: 763 137 928 41 4072 Fragments arranged by size: 4072 928 763 137 41 BbeI G_GCGC'C Cuts at: 2232 2232 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2625 2790 928 Size: 971 726 165 4079 Fragments arranged by size: 4079 971 726 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2360 2394 4132 4166 1903 Size: 34 423 34 1738 34 3678 Fragments arranged by size: 3678 1738 423 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2531 2882 4552 Size: 107 122 71 631 605 859 351 1670 Cuts at: 4552 136 Size: 1525 Fragments arranged by size: 1670 1525 859 631 605 351 122 107 71 BglII A'GATC_T Cuts at: 2100 5936 2100 Size: 3836 2105 BlpI GC'TnA_GC Cuts at: 3233 3358 3233 Size: 125 5816 Bme1580I G_kGCm'C Cuts at: 1790 2075 2154 2291 2390 2706 4002 5158 1790 Size: 285 79 137 99 316 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 316 285 137 99 79 BmgBI CAC'GTC Cuts at: 1318 2068 1318 Size: 750 5191 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 2774 2816 3077 4601 1239 Size: 1024 511 42 261 1524 2579 Fragments arranged by size: 2579 1524 1024 511 261 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2810 4066 4931 5229 2810 Size: 1256 865 298 3522 Fragments arranged by size: 3522 1256 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2582 4604 882 Size: 617 1083 2022 2219 Fragments arranged by size: 2219 2022 1083 617 BsaAI yAC'GTr Cuts at: 493 1204 2699 2920 3732 493 Size: 711 1495 221 812 2702 Fragments arranged by size: 2702 1495 812 711 221 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2175 2229 2349 Size: 53 83 186 936 612 30 54 120 Cuts at: 2349 2895 3748 4130 4765 275 Size: 546 853 382 635 1451 Fragments arranged by size: 1451 936 853 635 612 546 382 186 120 83 54 53 30 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5716 Fragments arranged by size: 5716 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2813 3023 1186 Size: 1627 210 4104 Fragments arranged by size: 4104 1627 210 BseYI C'CCAG_C Cuts at: 2291 2303 5144 5640 2291 Size: 12 2841 496 2592 Fragments arranged by size: 2841 2592 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2148 2359 2422 2492 1163 Size: 573 412 211 63 70 4612 Fragments arranged by size: 4612 573 412 211 70 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2318 3391 4152 5180 1540 Size: 275 186 208 109 1073 761 1028 2301 Fragments arranged by size: 2301 1073 1028 761 275 208 186 109 BsiHKAI G_wGCw'C Cuts at: 3219 3234 4002 4087 5158 3219 Size: 15 768 85 1071 4002 Fragments arranged by size: 4002 1071 768 85 15 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2154 2291 2390 2706 3219 3234 Size: 399 285 79 137 99 316 513 15 Cuts at: 3234 3260 4002 4087 5158 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 316 285 137 99 85 79 26 15 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2520 3296 844 Size: 1676 776 3489 Fragments arranged by size: 3489 1676 776 BsrBI CCG'CTC Cuts at: 1394 1823 2803 3828 1394 Size: 429 980 1025 3507 Fragments arranged by size: 3507 1025 980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 2204 2767 4585 4772 1700 Size: 110 388 6 563 1818 187 2869 Fragments arranged by size: 2869 1818 563 388 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 2183 1925 Size: 258 5683 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 2851 794 Size: 1721 336 3884 Fragments arranged by size: 3884 1721 336 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 3061 1184 Size: 1877 4064 BstYI r'GATC_y Cuts at: 1972 2100 2643 3271 3638 4023 4040 5492 5578 Size: 128 543 628 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 628 543 385 367 346 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2933 2933 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2126 2889 513 Size: 552 1061 763 3565 Fragments arranged by size: 3565 1061 763 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2376 2434 3457 4251 4271 2376 Size: 58 1023 794 20 4046 Fragments arranged by size: 4046 1023 794 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2498 4948 809 Size: 507 1182 2450 1802 Fragments arranged by size: 2450 1802 1182 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2196 2206 Size: 54 1425 50 275 18 228 138 10 Cuts at: 2206 2315 2883 3042 3388 4277 5906 8 Size: 109 568 159 346 889 1629 43 Fragments arranged by size: 1629 1425 889 568 346 275 228 159 138 109 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2206 2315 3388 1537 Size: 275 394 109 1073 4090 Fragments arranged by size: 4090 1073 394 275 109 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 3871 1353 Size: 914 1604 3423 Fragments arranged by size: 3423 1604 914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3217 3217 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2263 2383 2483 2729 2774 2802 2816 Size: 393 631 120 100 246 45 28 14 Cuts at: 2816 3077 3167 3188 4004 4601 5388 5656 1239 Size: 261 90 21 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 631 597 393 268 261 246 120 100 90 45 28 21 14 EcoO109I rG'GnC_Cy Cuts at: 2131 2151 2288 2300 2458 3037 2131 Size: 20 137 12 158 579 5035 Fragments arranged by size: 5035 579 158 137 20 12 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2232 3145 3196 5088 1678 Size: 554 913 51 1892 2531 Fragments arranged by size: 2531 1892 913 554 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2939 2971 4582 4614 4656 Size: 32 135 32 1367 32 1611 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1611 1367 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2228 2228 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2247 2346 3117 5055 5239 1305 Size: 825 117 99 771 1938 184 2007 Fragments arranged by size: 2007 1938 825 771 184 117 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 3044 10 Size: 54 1425 343 228 984 2907 Fragments arranged by size: 2907 1425 984 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 3899 4258 4417 518 Size: 648 900 1833 359 159 2042 Fragments arranged by size: 2042 1833 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2162 2764 2972 3357 4034 5182 5427 Size: 522 34 602 208 385 677 1148 245 Cuts at: 5427 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 602 522 385 245 208 34 NaeI GCC'GGC Cuts at: 2206 2206 Size: 5941 NarI GG'CG_CC Cuts at: 2229 2229 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2889 513 Size: 552 1824 3565 Fragments arranged by size: 3565 1824 552 NdeI CA'TA_TG Cuts at: 387 2055 387 Size: 1668 4273 NgoMIV G'CCGG_C Cuts at: 2204 2204 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 2315 3388 2315 Size: 1073 4868 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2383 3221 1348 Size: 1035 838 4068 Fragments arranged by size: 4068 1035 838 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2699 2699 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 2571 4010 4042 5523 5555 2539 Size: 32 1439 32 1481 32 2925 Fragments arranged by size: 2925 1481 1439 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2300 3037 2300 Size: 737 5204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2150 2287 2150 Size: 137 5804 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2764 2764 Size: 5941 SacI G_AGCT'C Cuts at: 3219 3219 Size: 5941 SacII CC_GC'GG Cuts at: 2129 2129 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2300 2300 Size: 5941 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2230 2230 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2286 2298 3268 1872 Size: 414 12 970 4545 Fragments arranged by size: 4545 970 414 12 SmlI C'TyrA_G Cuts at: 820 1017 1943 2825 4045 4946 5208 820 Size: 197 926 882 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SrfI GCCC'GGGC Cuts at: 2286 2286 Size: 5941 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2850 1517 Size: 1333 4608 StyI C'CwwG_G Cuts at: 513 1065 2448 2454 2889 3132 3380 513 Size: 552 1383 6 435 243 248 3074 Fragments arranged by size: 3074 1383 552 435 248 243 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 4187 5899 Size: 274 80 33 51 1443 852 1358 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1712 1443 1358 852 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3052 3052 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2284 2296 3266 1870 Size: 414 12 970 4545 Fragments arranged by size: 4545 970 414 12 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SwaI