(Linear) (Six-base) MAPSORT of: KIEE0591.seq Check: 6406 from: 1 to: 1236 KIEE0591.orf With 182 enzymes: SgfI * March 2, 2010 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 515 1236 Size: 515 721 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 378 478 724 797 1162 1183 1236 Size: 378 100 246 73 365 21 53 Fragments arranged by size: 378 365 246 100 73 53 21 AfeI AGC'GCT Cuts at: 0 1189 1236 Size: 1189 47 AflIII A'CryG_T Cuts at: 0 62 691 693 1236 Size: 62 629 2 543 Fragments arranged by size: 629 543 62 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 649 1236 Size: 649 587 AlwNI CAG_nnn'CTG Cuts at: 0 1222 1236 Size: 1222 14 ApaI G_GGCC'C Cuts at: 0 149 286 1236 Size: 149 137 950 Fragments arranged by size: 950 149 137 ApoI r'AATT_y Cuts at: 0 661 1236 Size: 661 575 AvaI C'yCGr_G Cuts at: 0 149 279 291 1236 Size: 149 130 12 945 Fragments arranged by size: 945 149 130 12 BanI G'GyrC_C Cuts at: 0 196 223 1236 Size: 196 27 1013 Fragments arranged by size: 1013 196 27 BanII G_rGCy'C Cuts at: 0 149 286 1214 1236 Size: 149 137 928 22 Fragments arranged by size: 928 149 137 22 BbeI G_GCGC'C Cuts at: 0 227 1236 Size: 227 1009 BbsI GAAGACnn'nnnn_ Cuts at: 0 620 785 1236 Size: 620 165 451 Fragments arranged by size: 620 451 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 355 389 1236 Size: 355 34 847 Fragments arranged by size: 847 355 34 BglI GCCn_nnn'nGGC Cuts at: 0 526 877 1236 Size: 526 351 359 Fragments arranged by size: 526 359 351 BglII A'GATC_T Cuts at: 0 95 1236 Size: 95 1141 BlpI GC'TnA_GC Cuts at: 0 1228 1236 Size: 1228 8 Bme1580I G_kGCm'C Cuts at: 0 70 149 286 385 701 1236 Size: 70 79 137 99 316 535 Fragments arranged by size: 535 316 137 99 79 70 BmgBI CAC'GTC Cuts at: 0 63 1236 Size: 63 1173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 769 811 1072 1236 Size: 258 511 42 261 164 Fragments arranged by size: 511 261 258 164 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 805 1236 Size: 805 431 BsaI GGTCTCn'nnnn_ Cuts at: 0 577 1236 Size: 577 659 BsaAI yAC'GTr Cuts at: 0 694 915 1236 Size: 694 221 321 Fragments arranged by size: 694 321 221 BsaHI Gr'CG_yC Cuts at: 0 140 170 224 344 890 1236 Size: 140 30 54 120 546 346 Fragments arranged by size: 546 346 140 120 54 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 808 1018 1236 Size: 808 210 218 Fragments arranged by size: 808 218 210 BseYI C'CCAG_C Cuts at: 0 286 298 1236 Size: 286 12 938 Fragments arranged by size: 938 286 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 143 354 417 487 1236 Size: 143 211 63 70 749 Fragments arranged by size: 749 211 143 70 63 BsiEI CG_ry'CG Cuts at: 0 204 313 1236 Size: 204 109 923 Fragments arranged by size: 923 204 109 BsiHKAI G_wGCw'C Cuts at: 0 1214 1229 1236 Size: 1214 15 7 Fragments arranged by size: 1214 15 7 Bsp1286I G_dGCh'C Cuts at: 0 70 149 286 385 701 1214 1229 1236 Size: 70 79 137 99 316 513 15 7 Fragments arranged by size: 513 316 137 99 79 70 15 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 515 1236 Size: 515 721 BsrBI CCG'CTC Cuts at: 0 798 1236 Size: 798 438 BsrFI r'CCGG_y Cuts at: 0 193 199 762 1236 Size: 193 6 563 474 Fragments arranged by size: 563 474 193 6 BssHII G'CGCG_C Cuts at: 0 178 1236 Size: 178 1058 BstAPI GCAn_nnn'nTGC Cuts at: 0 510 846 1236 Size: 510 336 390 Fragments arranged by size: 510 390 336 BstEII G'GTnAC_C Cuts at: 0 1056 1236 Size: 1056 180 BstYI r'GATC_y Cuts at: 0 95 638 1236 Size: 95 543 598 Fragments arranged by size: 598 543 95 Bsu36I CC'TnA_GG Cuts at: 0 928 1236 Size: 928 308 BtgI C'CryG_G Cuts at: 0 121 884 1236 Size: 121 763 352 Fragments arranged by size: 763 352 121 BtsI GCAGTG_nn' Cuts at: 0 371 429 1236 Size: 371 58 807 Fragments arranged by size: 807 371 58 DrdI GACnn_nn'nnGTC Cuts at: 0 493 1236 Size: 493 743 EaeI y'GGCC_r Cuts at: 0 53 191 201 310 878 1037 1236 Size: 53 138 10 109 568 159 199 Fragments arranged by size: 568 199 159 138 109 53 10 EagI C'GGCC_G Cuts at: 0 201 310 1236 Size: 201 109 926 Fragments arranged by size: 926 201 109 EarI CTCTTCn'nnn_ Cuts at: 0 262 1236 Size: 262 974 EcoICRI GAG'CTC Cuts at: 0 1212 1236 Size: 1212 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 378 478 724 769 797 811 1072 Size: 258 120 100 246 45 28 14 261 Cuts at: 1072 1162 1183 1236 Size: 90 21 53 Fragments arranged by size: 261 258 246 120 100 90 53 45 28 21 14 EcoO109I rG'GnC_Cy Cuts at: 0 126 146 283 295 453 1032 1236 Size: 126 20 137 12 158 579 204 Fragments arranged by size: 579 204 158 137 126 20 12 HaeII r_GCGC'y Cuts at: 0 227 1140 1191 1236 Size: 227 913 51 45 Fragments arranged by size: 913 227 51 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 934 966 1236 Size: 934 32 270 Fragments arranged by size: 934 270 32 KasI G'GCGC_C Cuts at: 0 223 1236 Size: 223 1013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 125 242 341 1112 1236 Size: 125 117 99 771 124 Fragments arranged by size: 771 125 124 117 99 MscI TGG'CCA Cuts at: 0 55 1039 1236 Size: 55 984 197 Fragments arranged by size: 984 197 55 MslI CAynn'nnrTG Cuts at: 0 61 1236 Size: 61 1175 MspA1I CmG'CkG Cuts at: 0 123 157 759 967 1236 Size: 123 34 602 208 269 Fragments arranged by size: 602 269 208 123 34 NaeI GCC'GGC Cuts at: 0 201 1236 Size: 201 1035 NarI GG'CG_CC Cuts at: 0 224 1236 Size: 224 1012 NcoI C'CATG_G Cuts at: 0 884 1236 Size: 884 352 NdeI CA'TA_TG Cuts at: 0 50 1236 Size: 50 1186 NgoMIV G'CCGG_C Cuts at: 0 199 1236 Size: 199 1037 NotI GC'GGCC_GC Cuts at: 0 310 1236 Size: 310 926 PasI CC'CwG_GG Cuts at: 0 378 1216 1236 Size: 378 838 20 Fragments arranged by size: 838 378 20 PfoI T'CCnGG_A Cuts at: 0 41 1236 Size: 41 1195 PmlI CAC'GTG Cuts at: 0 694 1236 Size: 694 542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 534 566 1236 Size: 534 32 670 Fragments arranged by size: 670 534 32 PpuMI rG'GwC_Cy Cuts at: 0 295 1032 1236 Size: 295 737 204 Fragments arranged by size: 737 295 204 PspOMI G'GGCC_C Cuts at: 0 145 282 1236 Size: 145 137 954 Fragments arranged by size: 954 145 137 PvuII CAG'CTG Cuts at: 0 759 1236 Size: 759 477 SacI G_AGCT'C Cuts at: 0 1214 1236 Size: 1214 22 SacII CC_GC'GG Cuts at: 0 124 1236 Size: 124 1112 SanDI GG'GwC_CC Cuts at: 0 295 1236 Size: 295 941 SapI GCTCTTCn'nnn_ Cuts at: 0 262 1236 Size: 262 974 SfoI GGC'GCC Cuts at: 0 225 1236 Size: 225 1011 SmaI CCC'GGG Cuts at: 0 281 293 1236 Size: 281 12 943 Fragments arranged by size: 943 281 12 SmlI C'TyrA_G Cuts at: 0 820 1236 Size: 820 416 SrfI GCCC'GGGC Cuts at: 0 281 1236 Size: 281 955 StuI AGG'CCT Cuts at: 0 845 1236 Size: 845 391 StyI C'CwwG_G Cuts at: 0 443 449 884 1127 1236 Size: 443 6 435 243 109 Fragments arranged by size: 443 435 243 109 6 TatI w'GTAC_w Cuts at: 0 824 1236 Size: 824 412 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1047 1236 Size: 1047 189 XmaI C'CCGG_G Cuts at: 0 279 291 1236 Size: 279 12 945 Fragments arranged by size: 945 279 12 Enzymes that do cut: AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI PasI PfoI PmlI PpiI PpuMI PspOMI PvuII SacI SacII SanDI SapI SfoI SmaI SmlI SrfI StuI StyI TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmrI BmtI BplI Bpu10I BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NheI NruI NsiI NspI PacI PciI PflMI PmeI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI