RAPGEF5 ORF clone pF1KSDA0277

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Flexi ORF Clone pF1KSDA0277

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Clone info.

Species

human

Clone Name

pF1KSDA0277 Sequence (with vector) Restriction Map

Product ID

FXC00490

HaloTag Clone PID

FHC00490

ORF Sequence

DNA sequence (1740 bp)
Protein sequence (580 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pF1KM Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383833]

Gene

KIAA0277

Symbol

RAPGEF5

Alias : GFR, MR-GEF, MRGEF, REPAC

Description

Rap guanine nucleotide exchange factor (GEF) 5

Original Clone ID

ha06833

Features of the cloned ORF sequence

Description

Length: 1740 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 580 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55093.1 3.5e-207 95.4 RAPGEF5 CCDS74604.1 3.9e-94 47.2 RAPGEF4 CCDS63061.1 4.1e-94 47.2 RAPGEF4 CCDS63060.1 4.2e-94 47.2 RAPGEF4 CCDS42776.1 4.2e-94 47.2 RAPGEF4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000651 72 173 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 344 525 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR000651 67 201 SM00229 Ras-like guanine nucleotide exchange factor IPR001895 341 580 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR000651 68 201 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 345 579 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011513954 554 609527 95.4 93.3 XP_005249973 730 609527 95.4 93.3 NP_036426 730 609527 95.4 93.3 XP_016868326 758 609527 95.4 93.3 XP_005249971 883 609527 95.4 93.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KSDA0277 Download

>KIAA0277  1740 bp
ATGGGCAGCTCCCGGCTGAGGGTCTTTGACCCTCATTTGGAGAGGAAAGATTCCGCCGCG
GCGCTCTCAGACCGAGAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATC
GACGAGGAGGATGAGGACGATGAATGGAGCAGCCGTTCGCAGTCTTCCACCGAGGATGAC
TCAGTGGACTCTCTGCTCTCTGACAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATT
TTGGAGCACCTTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACC
CTCCTGGATGACTTCCTTCTCACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAG
GCTCTGTTAAGGCACTATTCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCAGATGTT
CCGCGTAGGAAACGTAAAGTCTTGCATCTTGTTTCCCAGTGGATTGCTCTGTACAAAGAC
TGGTTACCTGAAGATGAACATTCAAAAATGTTTTTAAAGACCATATATAGGAATGTACTG
GATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAGATACTT
GGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAAGCCCTT
TTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACG
GAGGAAATTTTCTGCCACGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAA
GTTTCCAGTATAGCCCAAGAGATCCTAAAAGTCGTGGCAGAAAAGATCCAGTATGCAGAA
GAGGATCTGGCTCTGGTGGCCATCACATTCTCTGGGGAAAAGCATGAACTTCAGCCAAAT
GACTTAGTCATCTCCAAATCCCTCGAGGCATCTGGTCGAATATATGTCTACCGGAAAGAC
CTGGCGGACACTTTGAACCCATTTGCAGAAAATGAGGAATCACAGCAAAGGTCGATGAGG
ATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGGAGTCTA
TTCAATTCAATTCACGAGCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGAAGTGGG
GAACACACTGCAAATCTCAGCCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTTTGGGTG
GCCACGGAGATTCTGCTCTGCAGCCAGCTGGGCAAGCGAGTGCAGCTGGTGAAAAAATTC
ATCAAAATTGCGGCTCACTGCAAAGCCCAGAGAAACCTGAATTCTTTCTTTGCCATTGTG
ATGGGTCTCAACACTGCTTCTGTCAGTCGACTGTCGCAGACCTGGGAGAAAATCCCTGGG
AAGTTTAAGAAACTTTTCTCTGAACTTGAAAGTTTAACAGATCCTTCCCTAAATCACAAA
GCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATCCCTTTCATGCCCTTATTG
CTTAAAGATGTAACATTTATTCATGAAGGAAATAAAACTTTTTTGGATAATCTTGTCAAT
TTTGAAAAGCTGCATATGATCGCAGACACTGTCCGAACCCTGAGACACTGCAGGACTAAC
CAGTTTGGTGACCTGTCTCCAAAAGAGCATCAAGAGTTAAAGTCCTATGTTAATCACCTG
TATGTCATTGACAGCCAGCAGGCTCTGTTTGAGCTCTCACACAGGATCGAGCCTCGGGTG

ORF Protein

Cloned ORF protein sequence for pF1KSDA0277 Download

>KIAA0277  580 aa
MGSSRLRVFDPHLERKDSAAALSDRELPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDD
SVDSLLSDRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQ
ALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVL
DDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTET
EEIFCHVYITEHSYVSVKAKVSSIAQEILKVVAEKIQYAEEDLALVAITFSGEKHELQPN
DLVISKSLEASGRIYVYRKDLADTLNPFAENEESQQRSMRILGMNTWDLALELMNFDWSL
FNSIHEQELIYFTFSRQGSGEHTANLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKF
IKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHK
AYRDAFKKMKPPKIPFMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTN
QFGDLSPKEHQELKSYVNHLYVIDSQQALFELSHRIEPRV

ORF+Vector

Nucleotide Sequence (with vector) for pF1KSDA0277 Download

>pF1KSDA0277  4879 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCTTTCGAAGGAGAT
AGAACCATGGGCAGCTCCCGGCTGAGGGTCTTTGACCCTCATTTGGAGAGGAAAGATTCC
GCCGCGGCGCTCTCAGACCGAGAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAA
TACATCGACGAGGAGGATGAGGACGATGAATGGAGCAGCCGTTCGCAGTCTTCCACCGAG
GATGACTCAGTGGACTCTCTGCTCTCTGACAGATATGTGGTGGTGTCCGGGACCCCGGAG
AAGATTTTGGAGCACCTTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACA
GAGACCCTCCTGGATGACTTCCTTCTCACGTACACTGTCTTCATGACAACTGATGACTTG
TGCCAGGCTCTGTTAAGGCACTATTCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCA
GATGTTCCGCGTAGGAAACGTAAAGTCTTGCATCTTGTTTCCCAGTGGATTGCTCTGTAC
AAAGACTGGTTACCTGAAGATGAACATTCAAAAATGTTTTTAAAGACCATATATAGGAAT
GTACTGGATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAG
ATACTTGGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAA
GCCCTTTTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACT
GAAACGGAGGAAATTTTCTGCCACGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAG
GCAAAAGTTTCCAGTATAGCCCAAGAGATCCTAAAAGTCGTGGCAGAAAAGATCCAGTAT
GCAGAAGAGGATCTGGCTCTGGTGGCCATCACATTCTCTGGGGAAAAGCATGAACTTCAG
CCAAATGACTTAGTCATCTCCAAATCCCTCGAGGCATCTGGTCGAATATATGTCTACCGG
AAAGACCTGGCGGACACTTTGAACCCATTTGCAGAAAATGAGGAATCACAGCAAAGGTCG
ATGAGGATTTTGGGAATGAACACTTGGGATCTTGCTCTGGAATTAATGAATTTTGATTGG
AGTCTATTCAATTCAATTCACGAGCAAGAGCTGATCTACTTCACGTTCAGCAGACAGGGA
AGTGGGGAACACACTGCAAATCTCAGCCTTCTGCTCCAGAGATGCAATGAGGTCCAGCTT
TGGGTGGCCACGGAGATTCTGCTCTGCAGCCAGCTGGGCAAGCGAGTGCAGCTGGTGAAA
AAATTCATCAAAATTGCGGCTCACTGCAAAGCCCAGAGAAACCTGAATTCTTTCTTTGCC
ATTGTGATGGGTCTCAACACTGCTTCTGTCAGTCGACTGTCGCAGACCTGGGAGAAAATC
CCTGGGAAGTTTAAGAAACTTTTCTCTGAACTTGAAAGTTTAACAGATCCTTCCCTAAAT
CACAAAGCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATCCCTTTCATGCCC
TTATTGCTTAAAGATGTAACATTTATTCATGAAGGAAATAAAACTTTTTTGGATAATCTT
GTCAATTTTGAAAAGCTGCATATGATCGCAGACACTGTCCGAACCCTGAGACACTGCAGG
ACTAACCAGTTTGGTGACCTGTCTCCAAAAGAGCATCAAGAGTTAAAGTCCTATGTTAAT
CACCTGTATGTCATTGACAGCCAGCAGGCTCTGTTTGAGCTCTCACACAGGATCGAGCCT
CGGGTGTACGTAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACC
TGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC
TGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGG
TTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTC
TAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTC
CCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACT
GGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCG
TGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAA
GTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGAT
CAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCT
CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGC
TCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACC
GACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCC
ACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGG
CTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAG
AAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGC
CCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGT
CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTC
GCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCC
TGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGG
CTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAG
CTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCG
CAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCG
CGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGG
TTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG
GCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC
GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGA
TACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTT
ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGC
TGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCC
CCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTA
AGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT
GTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACA
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCT
TGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATT
ACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCT
CAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTC
ACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGG
ATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTA
CCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTT
TTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTT
AGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCAT
CGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTC
AGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGC
GGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGC
GCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAA
ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC
TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGG
AGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCAT
CCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATT
CAAATATGTATCCGCTCAT