Restriction map for pF1KSDA0277
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AviII       AvrII       BamHI       BanI        BbeI        
BcgI        BciVI       BclI        BfaI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PflMI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        StyI        SwaI        
Van91I      XbaI        XcmI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflII         676  CTTAAG               C^TTAAG
AflIII        737  ACGTGT               A^C[GA][CT]GT
AseI         1056  ATTAAT               AT^TAAT
AsnI         1056  ATTAAT               AT^TAAT
BanII        1711  GAGCTC               G[GA]GC[CT]^C
BbrPI         736  CACGTG               CAC^GTG
BbvCI          15  GCTGAGG              CCTCAGC(-5/-2)
BfrI          676  CTTAAG               C^TTAAG
BglI         1223  GCCAGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI         950  ACCGGA               [AT]^CCGG[AT]
BseRI         124  GAGGAG               GAGGAG(10/8)
BsgI         1240  GTGCAG               GTGCAG(16/14)
BsmFI         223  GGGAC                GGGAC(10/14)
Bsp1407I      470  TGTACA               T^GTACA
BsrDI        1178  GCAATG               GCAATG(2/0)
BsrGI         470  TGTACA               T^GTACA
BssSI        1093  CACGAG               CACGAG(-5/-1)
BstXI        1313  CCATTGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           61  GCGC                 GCG^C
DraI          513  TTTAAA               TTT^AAA
DraII         223  GGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          838  GAAGAG               CTCTTC(1/4)
EcoO109I      223  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1299  GAATTC               G^AATTC
EcoT22I      1451  ATGCAT               ATGCA^T
HaeII          60  GGCGCT               [GA]GCGC^[CT]
HhaI           61  GCGC                 GCG^C
HinP1I         61  GCGC                 G^CGC
HincII       1346  GTCGAC               GT[CT]^[GA]AC
HindII       1346  GTCGAC               GT[CT]^[GA]AC
Ksp632I       838  GAAGAG               CTCTTC(1/4)
KspI           56  CCGCGG               CCGC^GG
NdeI         1573  CATATG               CA^TATG
NsiI         1451  ATGCAT               ATGCA^T
PaeR7I        922  CTCGAG               C^TCGAG
PmaCI         736  CACGTG               CAC^GTG
PmlI          736  CACGTG               CAC^GTG
PpuMI         223  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        1056  ATTAAT               AT^TAAT
SacI         1711  GAGCTC               GAGCT^C
SacII          56  CCGCGG               CCGC^GG
SalI         1346  GTCGAC               G^TCGAC
SspBI         470  TGTACA               T^GTACA
SspI          629  AATATT               AAT^ATT
XhoI          922  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI          946  GTCTAC               GT^[AC][GT]AC
             1346  GTCGAC              
AccII          57  CGCG                 CG^CG
              422  CGCG                
AspI          901  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1585  GACACTGTC           
AvaI          922  CTCGAG               C^[CT]CG[GA]G
             1733  CTCGGG              
AvaII         224  GGACC                G^G[AT]CC
             1185  GGTCC               
BalI          857  TGGCCA               TGG^CCA
             1199  TGGCCA              
BbsI          162  GTCTTC               GAAGAC(2/6)
              331  GTCTTC              
BpmI          691  CTCCAG               CTGGAG(16/14)
             1168  CTCCAG              
BpuAI         162  GTCTTC               GAAGAC(2/6)
              331  GTCTTC              
BsaAI         321  CACGTA               [CT]AC^GT[GA]
              736  CACGTG              
BsaI          295  GAGACC               GGTCTC(1/5)
             1324  GGTCTC              
BsmI          602  GAATGC               GAATGC(1/-1)
             1453  GCATTC              
BsoBI         922  CTCGAG               C^[CT]CG[GA]G
             1733  CTCGGG              
BspHI         335  TCATGA               T^CATGA
             1521  TCATGA              
BstEII        482  GGTTACC              G^GT[ACGT]ACC
             1627  GGTGACC             
BstPI         482  GGTTACC              G^GT[ACGT]ACC
             1627  GGTGACC             
BstUI          57  CGCG                 CG^CG
              422  CGCG                
Cfr13I        224  GGACC                G^G[ACGT]CC
             1185  GGTCC               
DsaI           56  CCGCGG               C^C[GA][CT]GG
             1202  CCACGG              
EaeI          857  TGGCCA               [CT]^GGCC[GA]
             1199  TGGCCA              
EcoO65I       482  GGTTACC              G^GT[ACGT]ACC
             1627  GGTGACC             
HaeIII        858  GGCC                 GG^CC
             1200  GGCC                
MluNI         857  TGGCCA               TGG^CCA
             1199  TGGCCA              
MscI          857  TGGCCA               TGG^CCA
             1199  TGGCCA              
MvnI           57  CGCG                 CG^CG
              422  CGCG                
PflFI         901  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1585  GACACTGTC           
PstI         1218  CTGCAG               CTGCA^G
             1608  CTGCAG              
PvuII        1225  CAGCTG               CAG^CTG
             1243  CAGCTG              
RcaI          335  TCATGA               T^CATGA
             1521  TCATGA              
Sau96I        224  GGACC                G^G[ACGT]CC
             1185  GGTCC               
SmlI          676  CTTAAG               C^T[CT][GA]AG
              922  CTCGAG              
Tth111I       901  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1585  GACACTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       BbvI        
BcnI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HapII       
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI        
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