Restriction map for pF1KSDA0277
 (Linear) (Six-base) MAPSORT of: KIAA0277.seq  Check: 2983  from: 1  to: 1740



KIAA0277.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AccI GT'mk_AC

Cuts at:      0     947    1347    1740 
   Size:        947     400     393

  Fragments arranged by size: 

                 947     400     393
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509     874    1740 
   Size:        509     365     866

  Fragments arranged by size: 

                 866     509     365
 
AflII C'TTAA_G

Cuts at:      0     676    1740 
   Size:        676    1064
 
AflIII A'CryG_T

Cuts at:      0     737    1740 
   Size:        737    1003
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395    1255    1740 
   Size:        395     860     485

  Fragments arranged by size: 

                 860     485     395
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     138     170    1740 
   Size:        138      32    1570

  Fragments arranged by size: 

                1570     138      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1588    1704    1740 
   Size:        363    1225     116      36

  Fragments arranged by size: 

                1225     363     116      36
 
ApoI r'AATT_y

Cuts at:      0     583     725    1062    1255    1299    1740 
   Size:        583     142     337     193      44     441

  Fragments arranged by size: 

                 583     441     337     193     142      44
 
AseI AT'TA_AT

Cuts at:      0    1057    1740 
   Size:       1057     683
 
AvaI C'yCGr_G

Cuts at:      0     922    1733    1740 
   Size:        922     811       7

  Fragments arranged by size: 

                 922     811       7
 
BanII G_rGCy'C

Cuts at:      0    1715    1740 
   Size:       1715      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     155     324    1740 
   Size:        155     169    1416

  Fragments arranged by size: 

                1416     169     155
 
BbvCI CC'TCA_GC

Cuts at:      0      16    1740 
   Size:         16    1724
 
BfrBI ATG'CAT

Cuts at:      0    1453    1740 
   Size:       1453     287
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1229    1740 
   Size:       1229     511
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     450    1740 
   Size:        450    1290
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     195     426    1740 
   Size:        195     231    1314

  Fragments arranged by size: 

                1314     231     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1153    1740 
   Size:        676     477     587

  Fragments arranged by size: 

                 676     587     477
 
Bpu10I CC'TnA_GC

Cuts at:      0      16    1740 
   Size:         16    1724
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289    1330    1740 
   Size:        289    1041     410

  Fragments arranged by size: 

                1041     410     289
 
BsaAI yAC'GTr

Cuts at:      0     323     738    1740 
   Size:        323     415    1002

  Fragments arranged by size: 

                1002     415     323
 
BsaWI w'CCGG_w

Cuts at:      0     950    1740 
   Size:        950     790
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     138     168    1740 
   Size:        138      30    1572

  Fragments arranged by size: 

                1572     138      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139    1740 
   Size:        139    1601
 
BseYI C'CCAG_C

Cuts at:      0    1227    1740 
   Size:       1227     513
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1261    1740 
   Size:       1261     479
 
BsiHKAI G_wGCw'C

Cuts at:      0     248     755    1715    1740 
   Size:        248     507     960      25

  Fragments arranged by size: 

                 960     507     248      25
 
BsmI GAATG_Cn'

Cuts at:      0     608    1453    1740 
   Size:        608     845     287

  Fragments arranged by size: 

                 845     608     287
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     755    1715    1740 
   Size:        248     507     960      25

  Fragments arranged by size: 

                 960     507     248      25
 
BspHI T'CATG_A

Cuts at:      0     335    1521    1740 
   Size:        335    1186     219

  Fragments arranged by size: 

                1186     335     219
 
BsrDI GCAATG_nn'

Cuts at:      0    1185    1740 
   Size:       1185     555
 
BsrGI T'GTAC_A

Cuts at:      0     470    1740 
   Size:        470    1270
 
BssSI C'ACGA_G

Cuts at:      0    1093    1740 
   Size:       1093     647
 
BstEII G'GTnAC_C

Cuts at:      0     482    1627    1740 
   Size:        482    1145     113

  Fragments arranged by size: 

                1145     482     113
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1320    1740 
   Size:       1320     420
 
BstYI r'GATC_y

Cuts at:      0     800     824     843    1041    1419    1740 
   Size:        800      24      19     198     378     321

  Fragments arranged by size: 

                 800     378     321     198      24      19
 
BtgI C'CryG_G

Cuts at:      0      56    1202    1740 
   Size:         56    1146     538

  Fragments arranged by size: 

                1146     538      56
 
BtsI GCAGTG_nn'

Cuts at:      0    1145    1275    1331    1605    1740 
   Size:       1145     130      56     274     135

  Fragments arranged by size: 

                1145     274     135     130      56
 
DraI TTT'AAA

Cuts at:      0     515    1740 
   Size:        515    1225
 
EaeI y'GGCC_r

Cuts at:      0     857    1199    1740 
   Size:        857     342     541

  Fragments arranged by size: 

                 857     541     342
 
EarI CTCTTCn'nnn_

Cuts at:      0     833    1740 
   Size:        833     907
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42     979    1740 
   Size:         42     937     761

  Fragments arranged by size: 

                 937     761      42
 
EcoICRI GAG'CTC

Cuts at:      0    1713    1740 
   Size:       1713      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509     676     874    1153    1740 
   Size:        509     167     198     279     587

  Fragments arranged by size: 

                 587     509     279     198     167
 
EcoO109I rG'GnC_Cy

Cuts at:      0     224    1740 
   Size:        224    1516
 
EcoRI G'AATT_C

Cuts at:      0    1299    1740 
   Size:       1299     441
 
HaeII r_GCGC'y

Cuts at:      0      64    1740 
   Size:         64    1676
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1645    1677    1716    1740 
   Size:       1645      32      39      24

  Fragments arranged by size: 

                1645      39      32      24
 
HincII GTy'rAC

Cuts at:      0    1348    1740 
   Size:       1348     392
 
MscI TGG'CCA

Cuts at:      0     859    1201    1740 
   Size:        859     342     539

  Fragments arranged by size: 

                 859     539     342
 
MslI CAynn'nnrTG

Cuts at:      0     758    1318    1679    1740 
   Size:        758     560     361      61

  Fragments arranged by size: 

                 758     560     361      61
 
MspA1I CmG'CkG

Cuts at:      0      58    1227    1245    1740 
   Size:         58    1169      18     495

  Fragments arranged by size: 

                1169     495      58      18
 
NdeI CA'TA_TG

Cuts at:      0    1574    1740 
   Size:       1574     166
 
NsiI A_TGCA'T

Cuts at:      0    1455    1740 
   Size:       1455     285
 
PasI CC'CwG_GG

Cuts at:      0    1375    1740 
   Size:       1375     365
 
PfoI T'CCnGG_A

Cuts at:      0     220     278     302    1740 
   Size:        220      58      24    1438

  Fragments arranged by size: 

                1438     220      58      24
 
PmlI CAC'GTG

Cuts at:      0     738    1740 
   Size:        738    1002
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     138     170    1740 
   Size:        138      32    1570

  Fragments arranged by size: 

                1570     138      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     224    1740 
   Size:        224    1516
 
PspXI vC'TCGA_Gb

Cuts at:      0     922    1740 
   Size:        922     818
 
PstI C_TGCA'G

Cuts at:      0    1222    1612    1740 
   Size:       1222     390     128

  Fragments arranged by size: 

                1222     390     128
 
PvuII CAG'CTG

Cuts at:      0    1227    1245    1740 
   Size:       1227      18     495

  Fragments arranged by size: 

                1227     495      18
 
SacI G_AGCT'C

Cuts at:      0    1715    1740 
   Size:       1715      25
 
SacII CC_GC'GG

Cuts at:      0      59    1740 
   Size:         59    1681
 
SalI G'TCGA_C

Cuts at:      0    1346    1740 
   Size:       1346     394
 
SanDI GG'GwC_CC

Cuts at:      0     224    1740 
   Size:        224    1516
 
SfcI C'TryA_G

Cuts at:      0     620    1218    1443    1608    1740 
   Size:        620     598     225     165     132

  Fragments arranged by size: 

                 620     598     225     165     132
 
SmlI C'TyrA_G

Cuts at:      0     676     922    1740 
   Size:        676     246     818

  Fragments arranged by size: 

                 818     676     246
 
SspI AAT'ATT

Cuts at:      0     631    1740 
   Size:        631    1109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      86    1185    1740 
   Size:         86    1099     555

  Fragments arranged by size: 

                1099     555      86
 
TatI w'GTAC_w

Cuts at:      0     470     534    1740 
   Size:        470      64    1206

  Fragments arranged by size: 

                1206     470      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1463    1495    1740 
   Size:       1463      32     245

  Fragments arranged by size: 

                1463     245      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     904    1588    1740 
   Size:        904     684     152

  Fragments arranged by size: 

                 904     684     152
 
XhoI C'TCGA_G

Cuts at:      0     922    1740 
   Size:        922     818
 
XmnI GAAnn'nnTTC

Cuts at:      0    1255    1303    1393    1740 
   Size:       1255      48      90     347

  Fragments arranged by size: 

                1255     347      90      48
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI     ApoI
     AseI     AvaI    BanII     BbsI    BbvCI    BfrBI     BglI     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI
    BssSI   BstEII    BstXI    BstYI     BtgI     BtsI     DraI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     MscI     MslI   MspA1I     NdeI     NsiI     PasI     PfoI
     PmlI     PpiI    PpuMI    PspXI     PstI    PvuII     SacI    SacII
     SalI    SanDI     SfcI     SmlI     SspI    TaqII     TatI     TstI
  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmtI    BpuEI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PciI    PflMI     PmeI    PshAI
     PsiI   PspOMI     PsrI     PvuI    RsrII     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     XbaI     XcmI     XmaI
     ZraI
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