Gene/Protein Characteristic Table for KIAA0277
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Product ID ORK00490
Accession No D87467
Description Rap guanine nucleotide exchange factor (GEF) 5
Clone name ha06833
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5900 bp)
Predicted protein sequence (583 aa)
Flexi ORF Clone FXC00490
Source Human adult brain
Rouge ID mKIAA0277 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5900 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 4102 bp
Genome contig ID gi89161213r_22024447
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TGCTTTTCCAAATATGAGACTATGTTAAAGACACT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTCATGCCTCTTAGTTTCTTGCTTTCTCTGTGATGTGTGTGGATTAAAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 7 r 22124447 22199859 16 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 583 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q92565 0 100.0 Rap guanine nuc...
Homo sapiens
EAW93745 0 98.6 hCG38228, isofo...
Homo sapiens
XP_539466 0 97.2 similar to Rap ...
Canis lupus fam...
P83900 0 95.3 Rap guanine nuc...
Rattus norvegicus
AAH46627 0 94.8 Rapgef5 protein...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002311 3.2e-26 29.3 KIAA0313
AB002349 5.2e-12 27.1 KIAA0351
AB020653 1.5e-08 26.5 KIAA0846
AB023176 2.9e-05 27.9 KIAA0959
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000651 74 178 PF00618 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 345 529 PF00617 Guanine-nucleotide dissociation stimulator CDC25
HMMSmart IPR000651 70 204 SM00229 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 344 583 SM00147 Guanine-nucleotide dissociation stimulator CDC25
ProfileScan IPR000651 71 204 PS50212 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 348 582 PS50009 Guanine-nucleotide dissociation stimulator CDC25
ScanRegExp IPR001895 497 526 PS00720 Guanine-nucleotide dissociation stimulator CDC25
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 7
Experimental conditions
Panel name Genebridge 4
Primer_f AGCCCTGCGAGTCTGTTCTAG
Primer_r TGTTGGACCACTGCGGAAGAC
PCR product length 144 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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