GJA4 ORF clone FXC02277

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Flexi ORF Clone FXC02277

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Clone info.

Species

human

Clone Name

pF1KB7139 Sequence (with vector) Restriction Map

Product ID

FXC02277

HaloTag Clone PID

FHC02277

ORF Sequence

DNA sequence (999 bp)
Protein sequence (333 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463665]

Gene

KIBB7139

Symbol

GJA4

Alias : CX37

Description

gap junction protein, alpha 4, 37kDa

Original Clone ID

cp01144

Features of the cloned ORF sequence

Description

Length: 999 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 333 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30669.1 3e-118 99.7 GJA4 CCDS5123.1 2.2e-48 49.1 GJA1 CCDS9289.1 5.3e-48 46.8 GJA3 CCDS929.1 4e-45 45.8 GJA5 CCDS432.1 2.2e-42 52.7 GJA9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000500 21 45 PR00206 Connexin IPR000500 52 74 PR00206 Connexin IPR000500 77 97 PR00206 Connexin IPR002263 117 125 PR01134 Gap junction alpha-4 protein (Cx37) IPR000500 151 177 PR00206 Connexin IPR002263 153 166 PR01134 Gap junction alpha-4 protein (Cx37) IPR000500 187 207 PR00206 Connexin IPR000500 208 231 PR00206 Connexin IPR002263 252 259 PR01134 Gap junction alpha-4 protein (Cx37) IPR002263 293 300 PR01134 Gap junction alpha-4 protein (Cx37) HMMPfam IPR013092 3 108 PF00029 Connexin IPR019570 165 231 PF10582 Gap junction protein HMMSmart IPR013092 43 76 SM00037 Connexin
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 20 VVGKIWLTVLFIFRILILGLAGE 42 SECONDARY 23 2 77 YWVLQFLFVSTPTLVYLGHVIYL 99 PRIMARY 23 3 205 EKTIFIIFMLVVGLISLVLNLLE 227 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002051 333 121012 99.7 100.0 XP_005270807 333 121012 99.7 100.0 XP_016856532 333 121012 99.7 100.0 NP_068773 435 121015,601885 46.8 98.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KB7139 Download

>KIBB7139  999 bp
ATGGGTGACTGGGGCTTCCTGGAGAAGTTGCTGGACCAGGTCCAGGAGCACTCGACCGTG
GTGGGTAAGATCTGGCTGACGGTGCTCTTCATCTTCCGCATCCTCATCCTGGGCCTGGCC
GGCGAGTCAGTGTGGGGTGACGAGCAGTCAGATTTCGAGTGTAACACGGCCCAGCCAGGC
TGCACCAACGTCTGCTATGACCAGGCCTTCCCCATCTCCCACATCCGCTACTGGGTGCTG
CAGTTCCTCTTCGTCAGCACACCCACCCTGGTCTACCTGGGCCATGTCATTTACCTGTCT
CGGCGAGAAGAGCGGCTGCGGCAGAAGGAGGGGGAGCTGCGGGCACTGCCGGCCAAGGAC
CCACAGGTGGAGCGGGCGCTGGCGGCCGTAGAGCGTCAGATGGCCAAGATCTCGGTGGCA
GAAGATGGTCGCCTGCGCATCCGCGGAGCACTGATGGGCACCTATGTCGCCAGTGTGCTC
TGCAAGAGTGTGCTAGAGGCAGGCTTCCTCTATGGCCAGTGGCGCCTGTACGGCTGGACC
ATGGAGCCCGTGTTTGTGTGCCAGCGAGCACCCTGCCCCTACCTCGTGGACTGCTTTGTC
TCTCGCCCCACGGAGAAGACCATCTTCATCATCTTCATGTTGGTGGTTGGACTCATCTCC
CTGGTGCTTAACCTGCTGGAGTTGGTGCACCTGCTGTGTCGCTGCCTCAGCCGGGGGATG
AGGGCACGGCAAGGCCAAGACGCACCCCCGACCCAGGGCACCTCCTCAGACCCTTACACG
GACCAGGTCTTCTTCTACCTCCCCGTGGGCCAGGGGCCCTCATCCCCACCATGCCCCACC
TACAATGGGCTCTCATCCAGTGAGCAGAACTGGGCCAACCTGACCACAGAGGAGAGGCTG
GCGTCTTCCAGGCCCCCTCTCTTCCTGGACCCACCCCCTCAGAATGGCCAAAAATCCCCA
AGTCGTCCCAGCAGCTCTGCTTCTAAGAAGCAGTATGTA

ORF Protein

Cloned ORF protein sequence for pF1KB7139 Download

>KIBB7139  333 aa
MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPG
CTNVCYDQAFPISHIRYWVLQFLFVSTPTLVYLGHVIYLSRREERLRQKEGELRALPAKD
PQVERALAAVERQMAKISVAEDGRLRIRGALMGTYVASVLCKSVLEAGFLYGQWRLYGWT
MEPVFVCQRAPCPYLVDCFVSRPTEKTIFIIFMLVVGLISLVLNLLELVHLLCRCLSRGM
RARQGQDAPPTQGTSSDPYTDQVFFYLPVGQGPSSPPCPTYNGLSSSEQNWANLTTEERL
ASSRPPLFLDPPPQNGQKSPSRPSSSASKKQYV

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB7139 Download

>pF1KB7139  4112 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGGTGACTGG
GGCTTCCTGGAGAAGTTGCTGGACCAGGTCCAGGAGCACTCGACCGTGGTGGGTAAGATC
TGGCTGACGGTGCTCTTCATCTTCCGCATCCTCATCCTGGGCCTGGCCGGCGAGTCAGTG
TGGGGTGACGAGCAGTCAGATTTCGAGTGTAACACGGCCCAGCCAGGCTGCACCAACGTC
TGCTATGACCAGGCCTTCCCCATCTCCCACATCCGCTACTGGGTGCTGCAGTTCCTCTTC
GTCAGCACACCCACCCTGGTCTACCTGGGCCATGTCATTTACCTGTCTCGGCGAGAAGAG
CGGCTGCGGCAGAAGGAGGGGGAGCTGCGGGCACTGCCGGCCAAGGACCCACAGGTGGAG
CGGGCGCTGGCGGCCGTAGAGCGTCAGATGGCCAAGATCTCGGTGGCAGAAGATGGTCGC
CTGCGCATCCGCGGAGCACTGATGGGCACCTATGTCGCCAGTGTGCTCTGCAAGAGTGTG
CTAGAGGCAGGCTTCCTCTATGGCCAGTGGCGCCTGTACGGCTGGACCATGGAGCCCGTG
TTTGTGTGCCAGCGAGCACCCTGCCCCTACCTCGTGGACTGCTTTGTCTCTCGCCCCACG
GAGAAGACCATCTTCATCATCTTCATGTTGGTGGTTGGACTCATCTCCCTGGTGCTTAAC
CTGCTGGAGTTGGTGCACCTGCTGTGTCGCTGCCTCAGCCGGGGGATGAGGGCACGGCAA
GGCCAAGACGCACCCCCGACCCAGGGCACCTCCTCAGACCCTTACACGGACCAGGTCTTC
TTCTACCTCCCCGTGGGCCAGGGGCCCTCATCCCCACCATGCCCCACCTACAATGGGCTC
TCATCCAGTGAGCAGAACTGGGCCAACCTGACCACAGAGGAGAGGCTGGCGTCTTCCAGG
CCCCCTCTCTTCCTGGACCCACCCCCTCAGAATGGCCAAAAATCCCCAAGTCGTCCCAGC
AGCTCTGCTTCTAAGAAGCAGTATGTAGTTTAAACGAATTCGAGCTCGGTACCCGGGGAT
CCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGA
AGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAA
ACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCA
TAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTT
TGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCA
CCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGA
GTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTT
GGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGG
GGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGA
TTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAA
CAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTT
CTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGG
CTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAA
GCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCAC
CTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTT
GATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACT
CGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCG
CCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTG
ACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTC
ATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGT
GATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATC
GCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCG
GGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAA
GGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA
AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT
CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGAC
AGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCC
GACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTC
TCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG
TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGA
GTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAG
CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA
CACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAG
AGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTG
CAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTAC
GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATC
AAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATG
GCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGT
AATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCG
ATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCG
CTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTG
CCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGA
TCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACA
GAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGT
GAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCA
GGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTT
TGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGA
AGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTA
AGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCAT